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Effect of Mutations of Five Conserved Histidine Residues in the Catalytic Subunit of the cbb3 Cytochrome c Oxidase on its Function

  • Oh Jeong-Il
    • Journal of Microbiology
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    • v.44 no.3
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    • pp.284-292
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    • 2006
  • The cbb3 cytochrome c oxidase has the dual function as a terminal oxidase and oxygen sensor in the photosynthetic bacterium, Rhodobacter sphaeroides. The cbb3 oxidase forms a signal transduction pathway together with the PrrBA two-component system that controls photosynthesis gene expression in response to changes in oxygen tension in the environment. Under aerobic conditions the cbb3 oxidase generates an inhibitory signal, which shifts the equilibrium of PrrB kinase/phosphatase activities towards the phosphatase mode. Photosynthesis genes are thereby turned off under aerobic conditions. The catalytic subunit (CcoN) of the R. sphaeroides cbb3 oxidase contains five histidine residues (H2l4, B233, H303, H320, and H444) that are conserved in all CcoN subunits of the cbb3 oxidase, but not in the catalytic subunits of other members of copper-heme superfamily oxidases. H214A mutation of CcoN affected neither catalytic activity nor sensory (signaling) function of the cbb3 oxidase, whereas H320A mutation led to almost complete loss of both catalytic activity and sensory function of the cbb3 oxidase. H233V and H444A mutations brought about the partial loss of catalytic activity and sensory function of the cbb3 oxidase. Interestingly, the H303A mutant form of the cbb3 oxidase retains the catalytic function as a cytochrome c oxidase as compared to the wild-type oxidase, while it is defective in signaling function as an oxygen sensor. H303 appears to be implicated in either signal sensing or generation of the inhibitory signal to the PrrBA two-component system.

TOXIC EFFECT OF ADHESIVE RESINS ON THE DOG'S PULP TISSUE (접착성(接着性) 레진이 가견치수조직(家犬齒髓組織)에 미치는 독성(毒性)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Son, Ho-Hyun
    • Restorative Dentistry and Endodontics
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    • v.10 no.1
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    • pp.31-42
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    • 1984
  • The toxic effect of adhesive resins on the dog's pulp tissue was studied with 70 teeth from 5 dogs. The experimental materials were Clearfil, a mixture of Clearfil with calcium hydroxide powder, Panavia-EX, and a mixture of Panavia-EX with calcium hydroxide powder. As a control group, calcium hydroxide powder was used. Each material was placed on the pulpotomized tissue surface. After 3 days, 1, 2,4, and 6 weeks, the teeth and apical tissue were processed routinly and stained with hematoxylin and eosin. Pathological tissue changes due to the toxicity of adhesive resins were observed by light microscope, and the pH of Panavia-EX and the Bonding agent of Clearfil were measured. Following were the results; 1. In the group of calcium hydroxide powder, slight inflammatory change was observed in the pulpotomized surface and adjacent pulp tissue on 3 day. 1 week case showed incomplete dentin bridge. The remaining pulp tissue was normalized according to the days elapsed. 2. In the group of Clearfil, early inflammatory change revealed in the superificial portion of the remaining pulp tissue on 3 day. The inflammation spreaded over the total pulp tissue and partial necrosis was observed in 1 week and 2 week cases. Total necrosis of pulp tissue and moderate inflammatory change at the apical tissue was noticed in 4 week and 6 week cases. 3. In the group of Panavia-EX, moderate inflammatory change appeared in the superficial pulp tissue on 3 day, and severe inflammatory change over all pulp tissue found in 1 week case. Pulp necrosis was obvious in 2 week case. 4 week and 6 week cases were totally necrotized up to the periapical tissue. 4. In the groups of mixtures with calcium hydroxide powder, the pulp tissue destruction was retarded, compared with the groups of Clearfil and Panavia-EX. 5. Panavia-EX was more destructive than Clearfil. 6. The acidity of freshly mixed Bonding agent of Cleafil was pH 4.0, and that of Panavia-EX was pH 2.0.

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SULFIDATION PROCESSING AND Cr ADDITION TO IMPROVE OXIDATION RESISTANCE OF Ti-Al INTERMETALLIC COMPOUNDS AT ELEVATED TEMPERATURES

  • Narita, Toshio;Izumi, Takeshi;Yatagai, Mamoru;Yoshioka, Takayuki
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 1999.05a
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    • pp.5-5
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    • 1999
  • A novel process is proposed to improve oxidation resistance of Ti-Al intermetallic compounds at elevated temperatures by both Cr addition and pre-sulfidation, where TiAl alloys withlor without Cr addition were sulfidized at 1173K for 86.4ks at a 1.3 Pa sulfur partial pressure in a $H_2-H_2S$ gas mixture. The pre-sulfidation treatment formed a thin Cr-Al alloy layer as well as 7~10 micrometer $TiAl_3$ and $TiAl_2$ layer, due to selective sulfidation of Ti. Oxidation resistance of the pre-sulfidation processed TiAl 4Cr alloy was examined under isothermal and heat cycle conditions between room temperature and 1173K in air. Changes in $TiAl_3$ into $TiAl_2$ and then TiAl phases as well as their effect on oxidation behavior were investigated and compared with the oxidation behavior of the TiAl-4Cr alloy as TiAl and pre-sulfidation processed TiAl aHoys. After oxidation for up to 2.7Ms a protective $Al_2O_3$ scale was formed, and the pre-formed $TiAl_3$ changed into $TiAl_2$ and the $Al_2Cr$ phase changed into a CrAlTi phase between the $Al_2O_3$ scale and $TiAl_2$ layer. The pre-sulfidation processed TiAl-4Cr alloy had very good oxidation resistance for longer times, up to 2.7 Ms, in contrast to those observed for the pre-sulfidation processed TiAl alloy where localized oxidation occurred after 81 Oks and both the TiAl and TiAl-4Cr alloys themselves corroded rapidly from the initial stage of oxidation

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Formative characteristics of 3D printing fashion from the perspective of mechanic aesthetic (기계 미학적 관점에서 살펴본 3D Printing 패션의 조형적 특성)

  • Kim, Young-Sam;Lee, Jin-Ah;Kim, Jang-Hyeon;Jun, Yuh-Sun
    • The Research Journal of the Costume Culture
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    • v.23 no.2
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    • pp.294-309
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    • 2015
  • This study aims to clarify the aesthetic values between emotion of human and expression of technology in contemporary fashion as it analyzes formative characteristics of related cases in fashion based on principles of 3D Printing technology and the viewpoint of mechanic aesthetics. The conclusions of this study are as follows. First, 3D Printing fashion is not only expressed diverse variations by its principles of formative methods, materials and properties, but also changes of silhouette by applying system of designers. Second, general characteristics of 3D Printing fashion is represented by various applications in SLS system, and it can be specifically explained application to a portion of clothing, decorative roles of clothing, complicated pattern making through crossing fabrics using 3D scanner and displaying a certain object changing fashion styles, and so forth. Third, the formative characteristics of 3D Printing fashion from the perspective of mechanic aesthetics is as follows. It can be analyzed as the integration of metaphysical values through compared symbolization of natural feature and technical evolution, partial dynamics and interactive velocity-based, formative combinations for abstract expression using architectural components, cosmos images and substantialized structures through images of organic space interacted human shapes. As the mention above, 3D Printing technology can creative a diverse area of fashion, and express images of new technological fashion through various works with continuous development of techniques.

Spalling Resistance of High Strength Concrete Using Non-Stripping Form (비탈형 거푸집에 의한 고강도 콘크리트의 폭렬방지)

  • You, Ji-Young;Han, Chang-Pyung;Jee, Suk-Won;Han, Min-Cheol;Yang, Seong-Hwan;Han, Cheon-Goo
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.865-868
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    • 2008
  • As a part of a series of study, this study reviewed the fire proof characteristics of high performance concrete RC column members using non-stripping form that accompanied metal lath lateral confinement to prevent spalling of high performance concrete which is increasingly used recently, and the results are as follows. Flow and air amount both satisfied target range, and compressive strength, over 80MPa at age 28 days, showed high strength range. As for spalling characteristics, in the case of plain in which no fiber is mixed, severe spalling occurred, and in the case of 0.05% nylon("NY" hereinafter)+polypropylene("PP" hereinafter) fiber mixture, only surface area experienced partial spalling. Regarding non-stripping form changes, both non-stripping 25-20 and non-stripping 50-20 experienced spalling at finish material area, and non-stripping 50-20 showed better spalling proof performance than non-stripping 25-20. In the case of non-stripping 50-40, spalling was prevented, and while mass reduction rate was less than 10%, its temperature hysteresis showed the most excellent fire proof performance with base metal surface area maximum temperature $376.1^{\circ}C$.

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A Discussion of the Explanations of Gentrification - Focusing on Ley일s and Smith일s Works in the 1980s (젠트리피케이션에 관한 일고찰: 레이와 스미쓰의 1980년대 연구를 중심으로)

  • Pillsung, Byun
    • Journal of the Economic Geographical Society of Korea
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    • v.6 no.2
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    • pp.471-486
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    • 2003
  • Gentrification, the revitalization of inner city areas, encompasses both the changes in social and demographic composition and the alterations in the physical and economic nature of built environment. This urban phenomenon has received the bulk of attention since the 1970s, and simultaneously provided one prominent stage for the theoretical or ideological debates in the field of urban geography. Given this context, this paper deals with the consumption-based and production-based approaches to gentrification, both of which are involved in such debates. Specifically, this paper critically reviews and evaluates David Ley's and Neil Smith's explanations representing the two approaches, respectively. On the basis of the two urban geographers' seminal works published in the 1980s, this paper discusses the limitations of each explanation as a partial explanation.

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A Contrastive Study on the Change of Rural Houses of the Han Chinese and Korean-Chinese in Yanbian Area of China (중국연변지역 조선족과 한족 농촌주택의 변화에 관한 비교연구)

  • Heo, Seong-Geol;Cho, Won-Seok
    • Journal of the Korean Institute of Rural Architecture
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    • v.18 no.2
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    • pp.29-37
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    • 2016
  • This study is aimed to suggesting a hypothesis about the process of improvement of houses of the Korean-Chinese, comparing how Han Chinese and Korean-Chinese houses changed in Yanbian Area of China. This draws the conclusion by analyzing the field survey about residence of the Han Chinese and the Korean-Chinese in Yanbian Area of China. First of all, the characteristic of residence style change and the plan of rural houses of the Han Chinese are in the following. The plan of houses has been changed from allocating rooms from side to side with the kitchen as the center to linking each functional spaces through the aisle. And the main bedroom of houses becomes the central space for family members instead of Kang (partial Ondol) by expanding the floor heating system under the whole floor in houses. Next, the feature of residence style changes of the Han Chinese and the Korean-Chinese is as follows. In 1970s barns disappeared from houses of the Korean Chinese, but houses of the Han Chinese were not much changed. From 1980s to 1990s, spaces linking each rooms with aisles had appeared for comfortableness of residence and respecting each individual's privacy. On the other hand, The houses of the Korean-Chinese make a whole big space by getting rid of walls between the rooms. And after 2 thousand years later, Han Chinese houses have a bedroom with floor heating system. Both houses of the Korean-Chinese and the Han Chinese are changed to have a residence style which taking sedentary style and standing style.

A study of decomposition of sulfur oxides(harmful gas) using calcium dihydroxide catalyst by plasma reactions (Ca(OH)2촉매를 이용한 플라즈마 반응에 의한 황산화물(유해가스)의 제거에 관한 연구)

  • Kim, Dayoung;Hwang, Myungwhan;Woo, Insung
    • Journal of the Korea Safety Management & Science
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    • v.16 no.2
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    • pp.237-246
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    • 2014
  • Researches on the elimination of sulfur and nitrogen oxides with catalysts and absorbents reported many problems related with elimination efficiency and complex devices. In this study, decomposition efficiency of harmful gases was investigated. It was found that the efficiency rate can be increased by moving the harmful gases together with SPCP reactor and the catalysis reactor. Calcium hydroxide($Ca(OH)_2$), CaO, and $TiO_2$ were used as catalysts. Harmful air polluting gases such as $SO_2$ were measured for the analysis of decomposition efficiency, power consumption, and voltage according to changes to the process variables including frequency, concentration, electrode material, thickness of electrode, number of electrode winding, and additives to obtain optimal process conditions and the highest decomposition efficiency. The standard sample was sulfur oxide($SO_2$). Harmful gases were eliminated by moving them through the plasma generated in the SPCP reactor and the $Ca(OH)_2$ catalysis reactor. The elimination rate and products were analyzed with the gas analyzer (Ecom-AC,Germany), FT-IR(Nicolet, Magna-IR560), and GC-(Shimazu). The results of the experiment conducted to decompose and eliminate the harmful gas $SO_2$ with the $Ca(OH)_2$ catalysis reactor and SPCP reactor show 96% decomposition efficiency at the frequency of 10 kHz. The conductivity of the standard gas increased at the frequencies higher than 20 kHz. There was a partial flow of current along the surface. As a result, the decomposition efficiency decreased. The decomposition efficiency of harmful gas $SO_2$ by the $Ca(OH)_2$ catalysis reactor and SPCP reactor was 96.0% under 300 ppm concentration, 10 kHz frequency, and decomposition power of 20 W. It was 4% higher than the application of the SPCP reactor alone. The highest decomposition efficiency, 98.0% was achieved at the concentration of 100 ppm.

Effects of Convergence Cadaver Practice on Nursing Professionalism and Major Satisfaction in Nursing Students (간호대학생의 융합카데바 실습이 간호전문직관, 전공만족도에 미치는 영향)

  • Yang, In-Suk
    • Journal of Convergence for Information Technology
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    • v.10 no.3
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    • pp.38-47
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    • 2020
  • This study is designed as a one group pretest-posttest design the practice to investigate the effects of convergence cadaver practice on nursing professionalism and major satisfaction of nursing students. The participants were 133 nursing students who were in the anatomy course of M college from October 1 to October 30, 2018. The nursing professionalism resulted in changes from 3.50±.87 before the convergence cadaver practice to 3.44±.98 (t=.98, p<.330) after the convergence cadaver practice, and major satisfaction changed from 3.83±.81 before the convergence cadaver practice to 3.91±.78 (t=-.95, p<.345) after the convergence cadaver practice. The correlation between nursing professionalism and major satisfaction showed a positive correlation (r=.54, p>.001). Partial results of sub-areas showed increase, however, future studies that aim to develop and investigate the effects of specific convergence education program which includes cadaver practice to enhance qualitative structure of specialized foundation knowledge are needed.

The Development of a Healthy Sexuality Education Program for Early Adolescent (초기청소년의 건강한 성을 위한 프로그램 개발)

  • 윤명선;김순옥
    • Journal of the Korean Home Economics Association
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    • v.39 no.5
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    • pp.107-121
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    • 2001
  • The purpose of this study was to develop a sexuality education program for early adolescents. The overall goals of the program were to not only increase knowledge and skirts, but also to improve the awareness of healthy sex in order to promote premarital abstinence, parent-teen communication and parental awareness of the importance of parental roles and responsibilities in prevention of sexual problems of the early adolescent. The program consisted of six sessions: puberty changes during adolescence, the meaning of sexuality, sexual drives in dating relationships, understanding of the risks associated with early sexual involvement and support for postponing sexual activities, managing and avoiding sexually risky situations, the importance of communications with parents about issues related to sexuality. The program evaluation had three steps: pre-test, immediate post-test and two month follow-up post test. The results showed that the program had a significantly positive effect on the improvement of adolescent's knowledge about sex, and attitudes and behaviors toward sex, particularly in the immediate post-test. At the two month follow-up assessment, the effect of the program still remained strong, though there was a sight and partial reduction which was not statistically significant. Most of the program participants expressed a high level of satisfaction with the program. Parental involvement appeared to be effective as well. Recommendations for further study include: to re-evaluate the curriculum for various other participants. In addition, further study which would compare post-test results of these participants with a comparison group of peers who did not receive the program would be valuable. The author believes that the program can be used in implementing specific subjects (sessions) in new settings, as well as implementing the entire package of the program.

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