• Title/Summary/Keyword: paramagnetic

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Advanced techniques of solution nuclear magnetic resonance spectroscopy for structural investigation of protein-protein interaction

  • Sugiki, Toshihiko;Lee, Young-Ho
    • Journal of the Korean Magnetic Resonance Society
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    • v.22 no.4
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    • pp.76-81
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    • 2018
  • Investigation of the protein-protein interaction mode at atomic resolution is essential for understanding on the underlying functional mechanisms of proteins as well as for discovering druggable compounds blocking deleteriou interprotein interactions. Solution NMR spectroscopy provides accurate and precise information on intermolecular interactions even for weak and transient interactions, and it is also markedly useful for examining the change in the conformation and dynamics of target proteins upon binding events. In this mini-review, we comprehensively describe three unique and powerful methods of solution NMR spectroscopy, paramagnetic relaxation enhancement (PRE), pseudo-contact shift (PCS), and residual dipolar coupling (RDC), for the study on protein-protein interactions.

Preparation and Characterization of the Paramagnetic Rhenium Complex, $(CO)_4ReL_2$ [$L_2$= 2,3-Bis(diphenylphosphino)maleic anhydride]

  • Kim Dae-Soo;Chung Young-Baek;Kang Sang-Ook;Ko Jae-Jung
    • Bulletin of the Korean Chemical Society
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    • v.13 no.3
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    • pp.235-240
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    • 1992
  • The paramagnetic complex of $(CO)_4ReL_2[L_2=$ 2,3-Bis(diphenylphosphino)maleic anhydride] was prepared both by the photochemically induced homolytic splitting of $Re_2(CO)_10withL_2and$ by the chemical reduction of $[(CO)_4ReL_2]SO_3CF_3.$ The $(CO)_4ReL_2$ compound was characterized by ESR spectrum. The spectrum exhibits three group of sextets arising from one $^{185.187}Re$ nucleus (Ⅰ= 5/2) and two equivalent $^31P$ nuclei (Ⅰ= 1/2). Infrared, ESR, and cyclic voltammetric data are reported for all of the complexes prepared in this study.

Development of novel magnetic filter for paramagnetic particles in high gradient magnetic separation

  • Nishijima, Shigehiro;Nomura, Naoki
    • Progress in Superconductivity and Cryogenics
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    • v.24 no.3
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    • pp.7-11
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    • 2022
  • We are conducting research and development of magnetic filters for magnetic separation targeting paramagnetic materials. In order to develop a new magnetic filter with a large magnetic gradient, stainless fiber (SUS430, 120 mm × 3 mm) with a triangular cross section was sintered with a high void ratio (~ 70%) and the magnetic filter (20 mm × 2 mm) was created. When this magnetic filter was used to perform magnetic separation of hematite (particle size 50 ㎛) under a maximum magnetic flux density of 1.49 T, high separation rates were obtained.