• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.155-160
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• 1999

Studies on detoxification of Paralytic Shellfish Poison (PSP)-infested oyster, Crassostrea gigas were carried out using available processing resources. Changes of paralytic shellfish toxin components and specific toxicity during canning process were also investigated with high performance liquid chromatography (HPLC). Toxic oysters collected at Hachong in Koje Bay were used for experimental samples. The toxicity of oysters with range of 185-778 ug/100g was reduced below the quarantine limit of 80 ug/100g or not detected level by the mouse bioassay after canning process. The mole $\%$ of toxin components in the shucked oyster was in the order of 25.1 mole $\%$ of gonyautoxin 1, 19.2 mole $\%$ of gonyautoxin 3, 17.2 mole $\%$ of gonyautoxin 4 and 14.6 mole $\%$ of gonyautoxin 2. This sample had tracing amounts of Cl, C2, saxitoxin and neosaxitoxin. In the case of specific toxicity, the major toxins were consisted of gonyautoxin 1-4. The sum of gonyautoxin 1, 2, 3 and 4 was 80% of total toxicity of oyster. Saxitoxin and decarbamoylsaxitoxin were the more thermostable than any other toxin components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.6
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• pp.893-899
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• 1996

The studies on the detoxification of paralytic shellfish poison (PSP)-infested blue mussels, Mytilus edulis and oyster, Crassostrea gigas were performed for using of available processing resource. Toxic blue mussel and oysters from Nampo in Masan Bay, Hachong in Koje Bay and Woepori in Koje were used for experimental samples. The toxicity of low toxic blue mussel $(A,\;84{\mu}g/100g;\;B,\;166{\mu}g/100g;\;C,\;295{\mu}g/l00g;\;D,\;557{\mu}g/100g)$ and oyster $(740{\mu}g/100g)$ were reduced below the regulation limit of PSP $(80{\mu}g/100g)$ or undetected level by mouse bioassay after boiling at $98^{\circ}C$ for 10 min and retorting at $115^{\circ}C$ for 70 min, while the toxicity of high toxic blue mussel $(E,\;8,760{\mu}g/100g)$ remained beyond the regulation limit after boiling and retorting at same condition. These results suggested that the regulation limit of PSP could be level up from $(80{\mu}g/100g)$ to about $160{\mu}g/100g$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.1
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• pp.158-160
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• 1997

Food poisoning accident occurred on May, 1996 at Oepo, Geoje County, Kyeongnam Province, Korea, and two persons were died within a few hours after ingestion of the soup prepared with wild mussel, Mytilus edulis, harvested on the sea rock. Paralytic shellfish poisons (PSP) were elucidated as the responsible toxins for the food poisons accident because the wild mussels caught after three days at the near place from the accident contained high toxicity of PSP ranged $650\~1000MU/g$ of edible meat by mouse bioassay. Gonyautoxin-1+4 $(42.7\%)$ and C1+C2 $(40.0\%)$ were detected as the major toxins in the mussels by fluorometric HPLC method. Although, the poison extracted out with drip during freezing and thawing, and the toxicity gradually decreased by boiling for 20 minutes, over 30 MU/g of toxins remained in the soup and meat, which indicated that they could be able to make food poisoning.

• KSBB Journal
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• v.14 no.1
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• pp.1-8
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• 1999

It confirmed the facilitated diffusion of $Na^+$ of frog bladder membrane which is a tissue membrane. The mechanism was explained in $Na^+$ channel model and its referred to the $Na^+$ channel obstruction ingredient which was contained in the reference to the $Na^+$ channel obstruction ingredient and son on, e.g., seaweed, shellfish, pufferfish, phytoplankton and chinese drug. Also, it introduces the result which studied from the barrier point of the application of the tissue biosensor to the trade friction on Korea or Japan pufferfish and the marine environment in the one with high dependance. It was possible for the poison quantity of small amount pufferfish toxin (TTX), paralytic shellfish poisoning (PSP) to be measured and also to measure poison quantity in the cultivation poisonous toxin phytoplankton individual. In future, as for this tissue biosensor, it expects that it is possible to contribute widely until environment watch and also monitoring to the scene.

• Journal of Preventive Medicine and Public Health
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• v.21 no.1 s.23
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• pp.163-171
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• 1988

The paralytic shellfish poisoning was occurred among 25 laborers who worked at breaking-up of ships in Pusan for 5 days from March 29 to April 2 of 1956. For the purpose of accurately defining the paralytic shellfish poison(PSP) , the authors carried out mouse bioassay and chemical analysis. The results were summarized as follows: 1. The mean amount of Paralytic shellfish toxin was $1,207.8{\mu}g$ Per 100gm meat, and the mean death time of mouse was 5 minutes 16 second. 2. The properties of the PSP were mainly gonyautoxin group by chemical analysis(TLC, IR, $^{1}H-NMR$).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.2
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• pp.147-153
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• 2013

To prevent paralytic shellfish poisoning (PSP) due to the consumption of shellfish contaminated with PSP toxins, the quantitative analysis of these toxins is very crucial. The AOAC International mouse bioassay (MBA) has been used widely for the routine monitoring of PSP toxins for more than 50 years. However, this method has low sensitivity and high limit of quantification (LOQ) and interferences from other components in the extract, and it cannot determine toxic profiles. Ethical problems also exist with the continued use of this live mouse assay. To establish an alternative method to the MBA used for PSP toxins analysis, we attempted to optimize the analysis conditions of a precolumn high-performance liquid chromatography (HPLC) oxidation method and succeeded in validating its accuracy and precision in quantifying PSP toxins. A clear peak and the isolation of PSP toxins were obtained by injecting the working standards of Certified Reference Materials using HPLC. The LOQ of the precolumn HPLC oxidation method for PSP toxins was about $0.1002{\mu}g/g$, which represented an approximately fourfold improvement in detection capability versus the AOAC MBA. The intra-accuracy and precision for PSP toxins in oysters were 77.0-103.3% and 2.0-5.7%, respectively, while the respective inter-accuracy and precision were 77.3-100.7% and 2.4-6.0%. The mean recoveries of PSP toxins from oysters were 75.2-112.1%. The results of a comparison study showed good correlation between the results of the precolumn HPLC oxidation method and those of MBA, with a correlation factor of 0.9291 for mussels. The precolumn HPLC oxidation method may be used as an alternative to, or supplementary method with, MBA to monitor the occurrence of PSP toxins and to analyze the profiles of these toxins in shellfish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.546-549
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• 2000

For the establishment of biodetoxification method which can be acceptable for live bivalves, paralytic shellfish poison (PSP) detoxification bacteria were isolated from sea water and bivalves, and PSP detoxification activity and optimal growth condition of the isolated strains were investigated. from the bivalve and sea water samples, 8 strains of PSP detoxification bacteria were isolated. Of the isolated strains, CW-6 isolated from sea water shown strong PSP detoxification activity and decomposed completely 18 nmole/g of GTX2 after 3 days incubation in artificial medium. The selected stain CW-6 shown typical characteristics of the Enterobacter sp. and identified as Enterobacter sp, CW-6. Optimal growth condition of the Enterobacter sp. CW-6 were $35^{\circ}C$, pH 7 and $NaCl 1{\%}$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.1
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• pp.51-60
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• 1990

Some species of dinoflagellates were considered as one of the causative organisms of PSP(Paralytic Shellfish Poison) or DSP(Diarrhetic Shellfish Poison). Fish and shellfish are intoxicated by feeding of toxic plankton, sometimes human is intoxicated by feeding on these intoxicated fish and shellfish. In past ten years, the physiological and ecological studies of the toxic plankton has been investigated for development of monitoring system and preventation and control measures of PSP. However, in our country still little is known on a research for the toxic dinoflagellates. This paper reviews the general biology, taxonomic problem, physioecology and culture method of the toxic planktons such as Protogony-aulax and Dinophysis.

• Journal of Food Hygiene and Safety
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• v.16 no.3
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• pp.247-250
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• 2001

In order to investigate the distribution of paralytic shellfish poison, we examined the toxicity during from February to October in 2000. Of 591 shellfish samples, 17(2.88%) samples were detected. Scapgarca broughtonii was highest collected 14.29%(2/14). In the monthly detection rate of PSP, April was hitest 13.3%(8/60), in the regional collecting rate, Cheon-nam coastal area was highest 3.82%(10/262), and in cases of imported area, China was 8.3%(1/12). Imported area as well as domestic area samples should be strengthen to examine enduringly.

• Journal of Food Hygiene and Safety
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• v.14 no.3
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• pp.265-269
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• 1999

Paralytic shellfish poison threatens public health most seriously from April to early May every year and gives adverse effects on export of these products. Major shellfish products exported were canned oyster, Crassostrea gigas and blue mussel, Mytilus edulis. Toxicities of canned shellfishes with toxin of low levels were mostly inactivated during the processing; in contrast, residual toxicity was of great concern with canned products from highly toxic shellfishes. This study was to provide basic data to establish food safety measure by evaluating the changes of toxicity during 2 year storage of canned products with toxic blue mussel and oyster. Any significant difference was not observed between two samples. Boiled can and smoked can showed inactivation of toxicity to some extent, whereas acidified can did not show reduction of toxicity even after 2 year storage. In case the initial toxicity of shellfish was high long term storage could not inactivate the toxicity of the canned product.