• 한국어류학회지
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• 제19권2호
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• pp.88-92
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• 2007

Marine birnavirus (MABV)에 무증상적으로 감염된 넙치 (Paralichthys olivaceus) 치어에 면역억제제의 일종인 Dexamathasone을 투여하였을 때 MABV의 감염강도에 영향을 미치는가를 조사하였다. Real time PCR 분석결과 dexamethasone을 투여한 그룹이 생리식염수를 주사한 그룹 및 no handling 그룹에 비해 유의적으로 낮은 Ct 값을 나타냈으며, 또한 semi-quantitative RT-PCR 분석결과에 있어서도 dexamethasone을 주사한 그룹이 대조구 그룹들에 비해 MABV 유전자가 유의적으로 높게 증폭되는 것으로 나타났다. 이러한 결과로부터 dexamethasone 투여가 넙치 치어에 감염된 MABV의 복제를 증가시킴을 확인하였다.

• 한국수산과학회지
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• 제53권5호
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• pp.740-751
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• 2020

In the aquaculture industry, few studies are analyzing big data for intrinsic meaning. Fishcare Laboratory (www.fishcare.kr) diagnostic data from 2016-2018 was analyzed for scuticociliatosis (caused by Miamiensis avidus) outbreak patterns in cultured olive flounder Paralichthys olivaceus in Jeju, Korea. The scuticociliatosis monthly occurrence ratio is reported in the summary table after preparing and filtering the basic dataset model. Nonparametric test results suggest differences in the water temperature, body length, and weight between groups with and without scuticociliatosis. Data distribution visualization revealed that shorter body length and lighter weight increased the occurrence of scuticociliatosis. The association rule mining technique was applied to determine the primary clinical signs of mixed scuticociliatosis and bacterial infections. Venn diagrams were used to report clinical signs and suggest commonalities. These results may help diagnose and treat fish and provide a decision-making reference.

• 한국수산과학회지
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• 제36권2호
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• pp.107-112
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• 2003

Effect of PCBs on the skin and gill development of olive flounder, Paralichthys olivaceus were investigated by histological methodology and morphometric data. The olive flounder were exposed to limit concentration of effulent of PCBs (3.0 ${\mu}g/L$) for 60 days. Skin development can be classified into four stages: SSEL (simple squamous epithelial layer), MCA (mucous cell appearance) & CCA (club cell appearance) DLA (dermal layer appearance), and SEL (startification of epidermal layer) stages. Gill development had five stages: GFA (gill filament appearance), IGFE (identification of early gill filament epithelial cell) MCA (mucous cell appearance), PCA (pillar cell appearance), and FGL (formation of gill lamella) stages. The periods of structural completion of skin and gill were 22-30 days and 23-30 days after hatching in the exposure group respectively. The process of development of skin and gill of the exposure group was very similar to that of the control group. Therefore, PCBs (3 ${\mu}g/L$) have no influence on the development of skin and gill in the larval stage of olive flounder.

• 한국수산과학회지
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• 제51권1호
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• pp.23-30
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• 2018

Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.

• 한국어병학회지
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• 제15권1호
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• pp.37-42
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• 2002

Effect of mercury (Hg) toxicity on survival, growth, feed efficiency and oxygen consumption were examined in the juvenile olive flounder. Paralichthys olivaceus. Fishes were exposed to sublethal concentrations of Hg ranging from 0 to 0.13mg/L for 6 weeks. Hg reduced survival rate in a concentration and exposure period-dependent way and suddenly reduction occurred at Hg concentrations greater than 0.05mg/L after 6 weeks. Growth rate and feed efficiency also significantly decreased at greater than 0.028 and 0.05 mg/L respectively. Oxygen consumption rate was significantly decreased to 25 and 32% than that of the control at the Hg concentration of 0.05 and 0.13 mg/L respectively. These results suggest that Hg toxicity inhibit physiological function including growth, feed efficiency and oxygen consumption in the juvenile olive flounder, resulting in survival failure at high concentration.

• 한국수산과학회지
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• 제36권6호
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• pp.614-618
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• 2003

This study was conducted to evaluate the effects of dietary medicinal herb residues (MHR) on the growth and body composition of olive flounder (Paralichthys olivaceus). Two replicate groups of olive flounder with initial mean body weight of $18.7\pm0.3\;g$ were fed 8:2 (raw fish and commercial compound meal ratio)MP diet containing $0\%,\;50\%,\;10\%,\;15\%\;and\;20\%$ MHR for 7 weeks. Weight gain and feed efficiency increased in the $5\%\;and\;10\%$ group as compared to the control group, whereas they decreased in the $15\%\;and\;20\%$ groups. Moisture, crude protein, and crude ash content of the whole body were not significantly different between experimental groups, whereas crude lipid content of the $5\%\;and\;10\%$ groups were significantly higher than that of the $10\%\;and\;20\%$ groups. The results indicate that the addition of $5\%\;and\;10\%$ MHR in 8:2 MP diet can improve growth and feed efficiency, but addition of more than $15\%$ were not effective.

• 한국수산과학회지
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• 제50권6호
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• pp.750-755
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• 2017

Flatfish Paralichthys olivaceus raised in biofloc system (mean length $27.6{\pm}3.1cm$, mean weight $280.4{\pm}26.5g$) were exposed for to different concentrations of ammonia (0, 8, 16, 32, and $64mg\;{NH_4}^+/L$) for 7 days. Following ammonia exposure, hematological parameters such as hemoglobin and hematocrit were significantly reduced. Plasma components such as magnesium, glucose, aspartate aminotransferase (AST), and alanine aminotransaminase (ALT) were significantly altered by ammonia exposure, whereas there were no significant changes in calcium, cholesterol, or total protein. Antioxidant responses, such as superoxide dismutase (SOD) and catalase (CAT) levels were significantly elevated following ammonia exposure. The results of this study indicate that ammonia exposure induces significant changes in hematological parameters and antioxidant responses in biofloc-reared Paralichthys olivaceus as a toxic response.

• 한국포장학회지
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• 제26권3호
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• pp.165-171
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• 2020

유통과정에서 균질한 저온을 유지하는 것은 식품의 유통기한을 결정하는 가장 중요한 요소 중 하나인 미생물 증식을 억제하는 핵심 기술이다. 신선수산물의 전과정에서 정확하고 적절한 온도관리가 보장되었을 때 높은 품질의 신선 제품을 소비자에게 공급할 수 있다. 이 논문은 슈퍼칠링 신선수산식품의 상업성을 확인하기 위하여 포장된 신선 광어(Paralichthys Olivaceus) 의 유통기한을 연구한 것이다. 유통기한 측정을 위하여 중량, 대장균 등 세균수, pH, 관능검사, 색상, 휘발성 염기질소 등이 조사되었다. 연구결과, 휘발성염기질소를 기준으로 슈퍼칠링 온도(0±1℃)에서의 유통기한이 6일인데 비하여 일반 냉장온도 (8±2℃)의 경우 1일에 불과하여 슈퍼칠링 유통의 가능성을 보여주었다. 상업화 추진을 위해서는 소비자 신뢰도를 높이고 경제성을 향상시킬 수 있도록 슈퍼칠링 기법과 포장시스템에 대한 추가적인 포장방법 연구가 필요한 것으로 사료된다.

• 한국해양생명과학회지
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• 제2권1호
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• pp.12-19
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• 2017

급격한 수온의 변화는 어류의 생리학적인 측면에서 스트레스를 유발한다. 본 연구에서는 넙치(Paralichthys olivaceus)로부터 각 수온별(9, 12, 15, 18 및 21℃) 조건에 따라 24 및 48시간 동안 노출시킨 후에, 혈액생리학적 분석, 스트레스 단백질로 알려진 Hsp70 mRNA 발현 및 산소 소비량을 조사하였다. 혈액학적 분석에서 hematocrit (Ht) 및 hemoglobin (Hb), 혈장 코티졸 및 글루코스의 변화, aspartate aminotransferase (AST) 및 alanine aminotransferase (ALT), NH₃, 삼투질농도(osmolality) 및 총단백질(total protein, TP)은 9℃ 및 12℃에서 다른 수온별 실험구에 비해 대부분의 항목에서 유의적인 차이를 보였다. Hsp70 mRNA 발현은 9℃ 및 12℃에서 다른 실험구에 비해 높은 발현량을 확인하였고, 산소소비량은 9℃ 및 12℃에서 21℃에 비해 낮았다. 이러한 결과는 넙치 종자의 장거리 수송을 위한 수온자료로 활용할 수 있다.

• Fisheries and Aquatic Sciences
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• 제10권1호
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• pp.8-15
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• 2007

Rhodopsin, a dim-light receptor, is a model system for the study of G protein-coupled receptors that transduce extracellular signals into cells. To study the molecular mechanisms of visual systems in fish, the rod opsin gene of olive flounder Paralichthys olivaceus was characterized. The full-length P. olivaceus opsin gene was obtained by PCR amplification of genomic DNA, as well as cDNA synthesis. A comparison of clones obtained from both methods indicated that the olive flounder rod opsin gene lacks introns. Sequence analysis of the opsin gene indicated that it contains a 1,056-bp open reading frame encoding 352 amino acids. The deduced amino acid sequence contains features of typical rod opsins, such as sites for Schiff's base formation (K296) and its counterion (E113), disulfide formation (C110 and C187), and palmitoylation (C322 and C323). An opsin sequence alignment showed the highest similarity between P. olivaceus and Solea solea (95.1%), followed by Hippoglossus hippoglossus (94.5%). An opsin phylogenetic tree revealed a close relationship between olive flounder and teleost rod opsins.