• Title/Summary/Keyword: paper disc test

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A Study on the Cosmetic Preservative Effects of Cornus officinalis seed Extracts (산수유 종자 추출물의 화장품 방부효능에 관한 연구)

  • Yang, Jae-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.33 no.2
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    • pp.333-341
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    • 2016
  • To evaluate the possibility as a natural preservative of Cornus officinalis seed extracts, we investigated the anti-microbial activity and preservation effect of cosmetics. Cornus officinalis seed extracted with 1,3-Butylene Glycol(1,3-BG) and Ethanol(EtOH). As a result, Cornus officinalis seed extracts showed anti-microbial activity at Staphylococcus aureus, Staphylococcus epidermidis, Propionibacterium acnes and Cornus officinalis seed 1,3-BG extracts(COS-A) showed $26.7{\pm}4.0mm$ clear zone at the $1000.0{\mu}l/ml$. Minimum inhibitory concentration of S. epidermidis was observed at Cornus officinalis seed EtOH extracts(COS-B) $25.0{\mu}l/ml$. The result of challenge test for preservation effect on cosmetics, S. aureus and S. epidermidis was 100% eliminated from emulsion containing COS-A and COS-B after 7 days. These results suggest that Cornus officinalis seed extracts may have possibility as the natural preservative instead of synthetic perservative on cosmetics.

A Study on the Evaluation of Antimicrobial Effect of Orostachys Japonicus A. Berger Ethyl Acetate Fraction (와송 에틸아세테이트 분획물의 항균효능 평가에 관한 연구)

  • Im, Eun Kyung;Yang, Jae Chan
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.2
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    • pp.488-496
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    • 2021
  • In this study, a study was conducted to utilize Orostachys japonica A. Berger EtOAc fraction extract as an antibacterial activity and cosmetic ingredient. As a result of measuring the antimicrobial activity of Orostachys japonica A. Berger EtOAc, the growth of S. aureus, S. epidermidis, and P. aeruginosa was inhibited. Among them, S. aureus was an extract of 18.35 ± 1.5 mm Orostachys japonica A. Berger EtOAc fraction at a concentration of 0.5 g / mL, showing superior antibacterial activity than methyl paraben (16.83 ± 1.0 mm), and was shown as a positive control. As a result of evaluating the MIC of the Orostachys japonica A. Berger EtOAc fraction extract through MIC measurement, the remaining strains excluding Candida. A showed a MIC of 17.5 mg/mL. As a result of evaluating the cosmetic preservation effect through the challenge test applied to the cosmetic emulsion formulation, the growth inhibitory effect of S. aureus in the emulsion containing 0.3% Orostachys japonica A. Berger EtOAc fraction extract 7 days after microbial inoculation was 100%.

Estimation of Friction Characteristics of Wet-type Multiple Disc Brakes for Axle of 14 Ton Class Wheel Excavator (14톤급 휠 굴삭기 차축용 습식 다판 디스크 브레이크의 마찰특성 평가)

  • Cho, Yon-Snag;Park, Heung-Sik;Hong, Sung-Jin;Choi, Byeung-Woon;Bae, Myung-Ho
    • Tribology and Lubricants
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    • v.23 no.6
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    • pp.312-317
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    • 2007
  • In general, a brake system of axle for heavy duty machine as a wheel excavator makes use of wet-type multiple disk brakes. These disk bakes are very important parts of heavy duty machine because they are advanced in durability and braking power, and can be designed compactly. Thus, we designed and made wet-type multiple disk brakes of axle for the 14ton class wheel excavator to be localization of these imported all. These disk brakes were made a comparative test with the existing disk brakes by the SAE No.2 dynamometer, and the friction characteristics as dynamic and static friction coefficient and wear depth of friction paper were measured.

Growth-Inhibiting Effects of Herb Plants on Human Intestinal Bacteria

  • Kim, Moo-Key;Park, Byeoung-Soo;Kim, Byung-Su;Lee, Hoi-Seon
    • Journal of Applied Biological Chemistry
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    • v.44 no.4
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    • pp.185-189
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    • 2001
  • Essential oils of 21 herb plant samples, using spectrophotometric and paper disc agar diffusion methods under anaerobic conditions, were tested in vitro for their growth-inhibiting activities against Bifidobacterium bifidum, B. longum, Lactobacillus casei, Clostridium perfringens, and Escherichia coli. The responses varied with bacterial strains and plant oils. At 10 mg/disk, all essential oils did not inhibit beneficial intestinal bacteria, except for the oil of Alpinia officinarum and Melaleuca alternifolia against L. casei. Due to their strong growth-inhibitory activities against C. perfringens, E. coli, and L. casei, the activites of nine oils were evaluated at low concentrations. In test with C. perfringens at 1 mg/disk, the oils of Amyris balsamifera, Curcuma longa, M. alternifolia, and Trachyspermum ammi showed moderate activities. Moderate activities against E. coli were observed with the oils of M. alternifolia and T. ammi. These results may be indications of at least one of the pharmacological actions of the four herb plants.

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Analysis on the Squeal Noise of Wheel Brake System for Tilting Train (틸팅차량용 휠 제동장치의 스퀼 소음 해석)

  • Cha, Jung-Kwon;Park, Yeong-Il
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.20 no.1
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    • pp.98-105
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    • 2010
  • Squeal, a kind of self-excited vibration, is generated by the friction between the disc and the friction materials. It occurs at the ending stage of the braking process, and radiates and audible frequency range of 1 kHz to 10 kHz. Squeal is generated from unstability because of the coupling between the translation and rotation of the system. This instability is caused by the follower force and follower force is normal component of the friction force. In this paper modal analysis of wheel brake system was performed in order to predict the squeal phenomenon. It was shown that the prediction of system instability is possible by FEM. A finite element model of that brake system was made. Some parts of a real brake was selected and modeled. Modal analysis method performs analyses of each brake system component. Experimental modal analysis was performed for each brake components and experimental results were compared with analytical results from FEM. To predict the dynamic unstability of a whole system, the complex eigenvalue analysis for assembly modeling of components confirmed by modal analysis is performed. The finite element models of the disk brake assembly have been constructed, and the squeal noise problems have been solved by complex eigenvalue analysis. The complex eigenvalue analysis results compared with real train test.

Antimicrobial Activity and Bactericidal Activity of Caesalpinia sappan L. Extract (소목 추출물의 항균력 및 살균소독력)

  • Lee, Jin-Young;Min, Kyung-Jin
    • Journal of Environmental Health Sciences
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    • v.37 no.2
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    • pp.133-140
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    • 2011
  • Antimicrobial activity and bactericidal activity of Caesalpinia sappan L. extracts were investigated against five food-borne pathogens, E. coli, S. aureus, S. typhimurium, B. cereus and L. monocytogenes. Methanol extract of Caesalpinia sappan L. revealed antimicrobial activities against five pathogens. In particular, by paper disc diffusion the highest activity was shown against L. monocytogenes. Antimicrobial activities of methanol extracts showed the most potent activities, but hexane fraction had no activity. Fractions of ethyl acetate and butanol turned out to have higher antimicrobial activities against Gram(+) bacteria than Gram(-) bacteria. The minimum inhibitory concentration against five food-borne pathogens was 1.563 mg/ml on Gram(+) bacteria and 3.125 mg/ml on Gram(-) bacteria. The result of antimicrobial activity in a shaking flask method showed that bacterial growth rate fell by more than 99.999% at 3.125 mg/ml of methanol extract. The highest rate of viable reduction (99.998%) was shown at 0.781 mg/ml of methanol extract against L. monocytogenes. After five minutes of reaction between test strains and methanol extracts, the growth rates of five kinds of bacteria were reduced by more than 99.999% at a concentration of 100 mg/ml. Therefore, it is suggested that methanol extracts of Caesalpinia sappan L. can be developed as a natural sanitizer or disinfectant.

Friction and Wear Characteristics of Silica/Epoxy Composites for various Particle Size (입자지름의 변화에 따른 실리카 복합재료의 마찰 및 마모 특성)

  • Koh, Sung-Wi;Kim, Hyung-Jin;Kim, Kae-Dong;Kim, Chang-Soo
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2006.11a
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    • pp.141-144
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    • 2006
  • In this study, the friction and wear characteristics of pure epoxy and silica-filled epoxy resin composites with average silica particle diameter of $6-33{\mu}m$ were investigated at ambient temperature by pin-on-disc friction test. The cumulative wear volume, friction coefficient and wear rate of these materials against SiC abrasive paper were determined experimentally. The cumulative wear volume tended to increase nonlinearly with increase of sliding distance and depended on diameter of the silica particle for all these composites. The sliding wear tests of the materials demonstrated that the friction coefficient and the wear rate of silica filled epoxy composites were lower than those of the pure epoxy. silica filled epoxy.

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Fungal-Sporulation Suppressing Substances Produced by Pseudomonas aeruginosa KMCS-1

  • Min, Bu-Yong;Shim, Jae-Young;Kim, Kun-Woo;Lee, Jong-Kyu;Choi, Hyung-Tae;Yoon, Kwon-Sang
    • Journal of Microbiology
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    • v.34 no.3
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    • pp.284-288
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    • 1996
  • Among the bacteria isolated from compost piles of cattle excretion in a pasture located at the suburbs of Chunchon city, Pseudomonas aeruginosa KMCS-1 was selected for the test of antifungal substances produced. Six fractions were separated by silica gel column chromatography, and then the antifungal activity of each fraction was assayed against Escherichia coli, Bacillus subtilis, Candida albicans, Rhizopus sp., Aspergillus nidulans, Coprinus cinereus, and Pyricularia oryzae by paper disc method. Two fractions showed significant suppressive activities against A. nidulans, C. cinereus, and P. oryzae however, their mycelial growth was not affected by neither of these fractions. Inhibitory activities of these fractions to sporulation was assayed at the concentration of 50. 25, 12. 5, and 6.25 $\mu$g/ml and the average inhibition rates against sporulation of A. nidulans, C. cinereus, and P. oryzae were 94.0, 98.3, and 77.9%, respectively. Further purification and analysis of active substances are now being conducted.

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Wear Properties of Epoxy Matrix Nanocomposites (에폭시 기지 나노복합재료의 마모 특성)

  • Kim, J.D.;Kim, H.J.;Koh, S.W.;Kim, Y.S.
    • Journal of Power System Engineering
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    • v.14 no.6
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    • pp.83-88
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    • 2010
  • The wear behavior of epoxy matrix composites filled with nano sized silica particles is discussed in this paper. Especially, the variation of the coefficient of friction and the wear resistance according to the change of apply load and sliding velocity were investigated for these materials. Wear tests of pin-on-disc mode were carried out and the wear test results exhibited as following ; The epoxy matrix composites showed lower coefficient of friction compared to the neat epoxy through the whole sliding distance. As increasing the sliding velocity the epoxy matrix composites indicated lower coefficient of friction, whereas the neat epoxy showed higher coefficient of friction as increasing the sliding velocity. The specific friction work of both materials were increased with apply load. In case of the epoxy matrix composites, the running in periods of friction were reduced as increase in apply load. The epoxy matrix composites were improved the wear resistance by adding the nano silica particles remarkably. It is expected that the load carrying capacity of the epoxy matrix composites will be improved by increase of Pv factor.

Antibacterial Activity against the Streptococcus mutans and Anti-inflammatory Effect of Lophatheri Herba (담죽엽의 충치균에 대한 항균활성 및 항염효과)

  • Jeon, Hoon;Park, Yeong-Seo;Kang, In-Tak;Cui, Xun;Lee, Tae-Kyoo;Kim, Hoon;Lim, Jong-Pil
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.20 no.6
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    • pp.1567-1571
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    • 2006
  • Lophatheri Herba of Lophatherum gracile Bronghiart(Gramineae) has long been used for treatment of inflammation, fever and edema in Korea. In order to investigate antibacterial activity of the Lophatheri Herba against Streptococcus mutans ATCC27351, paper disc test and pH check were carried out with 80% ethanol extract of Lophatheri Herba(LEX). The LEX showed significant antibacterial activity. And at the dose of 50 mg/kg, LEX showed signifiant inhibition on the paw edema, vascular permeability and myeloperoxidase activity in rat's paw tissue. These results indicate that LEX has antibacterial activity against the Streptococcus mutans and anti-inflammatory effect.