• Journal of Life Science
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• v.13 no.4
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• pp.541-550
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• 2003

E2 glycoprotein of hepatitis C virus (HCV) comprises a surface of viral particle together with E1 glycoprotein, and is thought to be involved in the attachment of HCV viral particle to receptor (s) on the permissible cells including hepatocytes, B cells, T cells, and monocytes. We constructed a phage library expressing cellular proteins of hepatocytes on the phage surface, which turned out to be 8.8${\times}$$10^5$ cfu of diversity and carried inserts in 95% of library. We screened both cDNA phage library and 12-mer peptide library to identify the cellular proteins binding to E2 protein. Some intracellular proteins including tensin and membrane band 4.1 which are involved in signal transduction of survival and cytoskeleton organization, were selected from cDNA phage library through several rounds of panning and screening. On the contrary, membrane proteins such as CCR7, CKR-L2, and insulin-like growth factor-1 receptor were identified through screening of peptide library. Phages expressing peptides corresponding to those membrane proteins were bound to E2 protein specifically as determined by neutralization of binding assay. Since it is well known that HCV can infect T cells as well as hepatocytes, we examined to see if E2 protein can bind to CCR7, a member of C-protein coupled receptor family expressed on T cells, using CCR7 transfected tells. Human CCR7 cDNA was cloned into pcDNA3.1(-) vector and transfected into human embryonic kidney cell, 293T, and expressed on the surface of the cell as shown by flow cytometer. Binding assay of E2 protein using CCR7 transfected cells indicated that E2 protein bound to CCR7 by dose-dependent mode, giving rise to the possibility that CCR7 might be a putative cellular receptor for HCV.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.362-373
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• 2021

This is an action research study of mixed methodology design to confirm the implementation process and effects of applying visual thinking and window paning on improving nursing ethics and professional courses. Based on the conceptual model for action research, a quantitative and qualitative approach was taken. The data was collected and analyzed in an integrated manner. The survey analysis was done using the SPSS WIN 23.0 program. The participants were interviewed after experiencing the techniques in class and content analysis was used on the answers. As a result of applying visual thinking and window paning, ethical decision-making confidence (t=6.748, p<.001) and nursing professional intuition (t=-3.52, p<.001) showed statistically significant changes. There was, however, no significant change in biomedical ethics consciousness (t=1.291, p=.199). Qualitative analysis found that they had fresh experience, an unfamiliar but comfortable feeling, feeling of being mine, insufficient time, systematic case study approach based on theory, were able to cultivate cooperation and coordination ability through discussion and experience in various professional fields, pride, ethical responsibility consciousness and were able to apply learning content in the field. Visual thinking and window paning foster diverse competencies in nursing education and help integrative learning. Therefore, based on the results it is proposed that visual thinking and window paning are applied to the improvement of instruction in other courses to develop core nursing competency.

• Journal of Microbiology
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• v.45 no.6
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• pp.528-533
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• 2007

In a previous study we generated an anti-Hepatitis B Virus (HBV) preS1 humanized antibody (HzKR127) that showed in vivo HBV-neutralizing activity in chimpanzees. However, the antigen-binding affinity of the humanized antibody may not be sufficient for clinical use and thus affinity maturation is required for better therapeutic efficacy. In this study, phage display technique was employed to increase the affinity of HzKR127. All six amino acid residues (Glu95-Tyr96-Asp97-Glu98-Ala99-Tyr100) in the heavy (H) chain complementary-determining region 3 (HCDR3) of HzKR127 were randomized and phage-displayed single chain Fv (scFv) library was constructed. After three rounds of panning, 12 different clones exhibiting higher antigen-binding activity than the wild type ScFv were selected and their antigen-binding specificity for the preS1 confirmed. Subsequently, five ScFv clones were converted to whole IgG and subjected to affinity determination. The results showed that two clones (B3 and A19) exhibited an approximately 6 fold higher affinities than that of HzKR127. The affinity-matured humanized antibodies may be useful in anti-HBV immunotherapy.

• Journal of KIISE:Software and Applications
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• v.27 no.5
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• pp.510-520
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• 2000

For content-based video indexing and retrieval, it is necessary to segment video data into video shots and then select key frames or representative frames for each shot. However, it is very difficult to select key frames automatically because the task of selecting meaningful frames is quite subjective. In this paper, we propose a new approach in selecting key frames based on visual contents such as region information and their temporal variations in the shot. First of all, we classify video shots into panning shots, zooming shots, tilting shots or no camera motion shots by detecting camera motion information in video shots. Then, in each category, we apply appropriate fuzzy rules to select key frames based on meaningful content in frame. Finally, we control the number of key frames in the selection process by adjusting the degree of detail in representing video shots.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.3
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• pp.358-379
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• 2010

Multiple object association is an important capability in visual surveillance system with multiple cameras. In this paper, we introduce locally initiating line-based object association with the parallel projection camera model, which can be applicable to the situation without the common (ground) plane. The parallel projection camera model supports the camera movement (i.e. panning, tilting and zooming) by using the simple table based compensation for non-ideal camera parameters. We propose the threshold distance based homographic line generation algorithm. This takes account of uncertain parameters such as transformation error, height uncertainty of objects and synchronization issue between cameras. Thus, the proposed algorithm associates multiple objects on demand in the surveillance system where the camera movement dynamically changes. We verify the proposed method with actual image frames. Finally, we discuss the strategy to improve the association performance by using the temporal and spatial redundancy.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.21 no.1
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• pp.3-8
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• 2011

This paper introduces a robot eye module, of which two degree-of-freedom motions, i.e. panning and tilting, are driven by a pair of wires. The main feature of the module is that each wire can generate push-pull motion without buckling. It is thanks to a Teflon tube which guides the path of the moving wire. End points of the tube and wire have pivot elements so that a smooth push-pull motion is produced even when the end point of wire is moved by eye rotation. This mechanism helps the eye module to be very compact. In this paper, the structure of the robot eye module is introduced in detail, and the required motor angles for a certain direction of eye line are investigated analytically and experimentally.

• The Journal of the Korea Contents Association
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• v.7 no.8
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• pp.1-12
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• 2007

This paper presents a new demixing method that separates each source from a stereo sound mixture. Under the W-Disjoint Orthogonal assumption in DUET(Degenerate Unmixing Estimation Technique) algorithm. The proposed method is mainly processed in time-frequency domain by using windowed-fourier transform. In this paper there are two main contributions: a weighted mask by panning index distances and a binary mask by comparing each channel value. The former has tender demixing characteristic, and the latter has stronger demixing characteristic. In experimental results, we will show that both masks produce more robust demixing than the existing demixing methods do.

• Journal of Broadcast Engineering
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• v.6 no.2
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• pp.160-168
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• 2001

In this paper, we propose a new practical motion model that can exploit the general properties of camera motion in constructing a panorama. accounting for panning. tilting, and evert the change in focal length of the camera. We also present an efficient algorithm to handle moving objects or noose in the scene based on outliers rejection. Spatial and temporal statistical properties of motion field are exploited to detect the outliers. The proposed algorithm removes moving objects or noise from the panoramic Image so that mode clear and complete view of the background Image can be obtained. This method does not require assumptions or a priors knowledge of the scene. The entire process is fully automatic as this method does not require any manual correction in the process of constructing a Panorama. The proposed algorithm is tested on the broadcasting images of soccer games. Oun simulation result shows that this method is superior to conventional image mosaicing algorithms.

• Journal of Ocean Engineering and Technology
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• v.23 no.5
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• pp.61-70
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• 2009

An AUV (Autonomous Underwater Vehicle) is an unmanned underwater vessel to investigate sea environments and deep sea resource. To be completely autonomous, AUV must have the ability to home and dock to the launcher. In this paper, we consider a class of homing trajectory planning problem for an AUV with kinematic and tactical constraints in horizontal plane. Since the AUV under consideration has underactuated characteristics, trajectory for this kind of AUV must be designed considering the underactuated characteristics. Otherwise, the AUV cannot follow the trajectory. Proposed homing trajectory panning method that called VGM (Virtual Goal Method) based on visibility graph takes the underactated characteristics into consideration. And it guarantees shortest collision free trajectory. For tracking control, we propose a PD controller by simple guidance law. Finally, we validate the trajectory planning algorithm and tracking controller by numerical simulation and ocean engineering basin experiment in KORDI.

• Korean Institute of Interior Design Journal
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• v.20 no.5
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• pp.51-58
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• 2011

As it gets nearer to the modern society, social and cultural situations are changing and the spatial form of public space is changing into the complex structure where it is interlinked with each other diversely. In particular, as the development of large-scale complex spreads nationwide, the form of complex commercial space assuming the nature of malls appears prominently. In such a large-scale complex commercial space, the diverse visual elements make the environment difficult to be perceived. Like this, the attention in the commercial space where a variety of visual stimuli are in the presence, is important element, so the plan of VP(visual presentation) is needed for effectively selecting or inducing the space. Thus, in this study, human visual and perception process will be reviewed and a method to evaluate the attention will be proposed. Also, the attention of each stores in the complex commercial spaces will be evaluated to propose the indicators for resonable spatial panning in the future.