• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 1990년도 Proceedings of International Symposium on Korean Ginseng, 1990, Seoul, Korea
• /
• pp.102-110
• /
• 1990

Antitumor polyacetylenlc alcohol, panaxytriol (Ci7 H2603), was isolated and purified from a Powder of the root of Panax ginseng C.A. Meyer. Panaxytriol Possesses unusual Property of being soluble in both water and organic solvents. Panaxytriol inhibited the growth of various kinds of human cultured cell lines in dose-dependent fashion in vitro. The in vivo effects of panaxytriol were tested against C57BU6 mice transplanted with Bl6 melanomas. Panaxytriol (8 and 40 mg/kg) administered intramuscularly (im) produced significant tumor growth delays in mice. Although a detailed mechanism of growth inhibition by panaxytriol is unknown, preliminary results appear to implicate a surface membrane site of action. And its action seems to be more dose-dependent than time-dependent. Finally, panaxytriol pharmacokinetics was evaluated in mice given single 8 mgrkg doses intraperitoneally(ip) or im. Serum panaxytriol content was measured using both tumor growth inhibitory assay and a gas chromatographic method. The maximum serum panaxytriol content after ip and im administration was 35.0 and 1.61 ug/ml respectively. These results indicate that the compound may act as cytotoxic substance even in-patients. Keywords Panax ginseng, panaxytriol, tumor growth inhibition

• Journal of Ginseng Research
• /
• 제14권2호
• /
• pp.244-252
• /
• 1990

Antitumor polyacetylenic alcohol, panaxytriol (Cl7 H26O3), was isolated and purified from a powder of the root of Pnnnx tin.1.encl C.A. Meyer. Panaxytriol possesses unusual property of being soluble in both water and organic solvents. Panaxytriol inhibited the growth of various kinds of human cultured cell lines in dose-dependent fashion in vitro. The in vivo effects of panaxytriol were tested against C57BL/6 mice transplanted with Bl6 melanomas. Panaxytriol (8 and 40 mg/kg) administered intra-muscularly(im) produced significant tumor growth delays in mice. Although a detailed mechanism of growth inhibition by panaxytriol is unknown, preliminary results appear to implicates a surface membrane site of action. And its action seems to be more dose-dependent than time-dependent. Finally, panaxytriol pharmacokinetics was evaluated in mice given single 8 mg/kg doses intraperitoneally (ip) or im. Serum panaxytriol content was measured using both tumor growth inhibitory assay and a gas chromatographic method. The maximum serum panaxytriol content after ip and im administration was 35.0 and 1.6 $\mu$g/ml respectively. These results indicate that the compound may act as cytotoxic substance even in patients.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 1988년도 학술대회지
• /
• pp.33-35
• /
• 1988

우리는 경험적으로 인삼이 훌륭한 치료효과로서 항종양효과가 있다는 것을 알고 있다. 인삼으로부터 종양발육 억제효과가 있는 물질을 분리하는 과정에서 우리는 새로운 타입의 항종양 물질을 발견하였다. 이물질은 Panax ginseng C.A. Mayer의 분말로부터 분리하였는데 이 분말은 일본에서 홍삼분말이라는 이름으로 의약품으로서 여러가지 질병치료에 사용되고 있다. IR, $^{1}H,\;^{13}C-NMR$ 및 MS에 의한 분석결과로 이 물질은 panaxytriol로 동정 되었다. 고려홍삼에서 분리된 panaxytriol은 in vitro 시험에서 몇가지의 인체암세포와 악성백혈병 세포의 성장을 억제하였다. 비록 panaxytriol에 의한 세포성장억제에 대한 상세한 작용기전은 알려져있지 않으나 panaxytriol의 효과는 처리시간보다는 농도 의존성이 있는 것으로 밝혀졌다.

• Journal of Ginseng Research
• /
• 제13권2호
• /
• pp.183-188
• /
• 1989

인삼의 주요 polyacetylens 화합물인 panaxynol, panaxydol 및 panaxytriol의 정량을 위한 GC 분석조건을 확립하였다. 이 때, GC column과 검출기는 SPB-1 fused silica capillary(0.25 mm i, d. $\times$30m, Supelco)와 불꽃이온화검출기(FID)를 사용하였으며 column oven의 온도는 $250^{\circ}C$ 등온이었다. Panaxynol, panaxydol 및 panaxytriol peaks의 retention time은 TMS 유도체화 전의 경우에는 각각 4.2, 5.1 및 6.9분이었으며 TMS유도체화 후에는 각각 4.5, 5.4 및 7.4 분이었다. TMS 유도체화 전의 panaxynol, panaxydol 및 panaxytriol의 정량가능 최소농도는 각각 20, 50 및 100ppm 이었던 데에 비하여, TMS 유도체화 후에는 세 화합물의 정량가능 최소농도가 모두 5ppm의 낮은 수준을 나타내었다. 한편, 홍삼 중의 panaxynol, panaxydol 및 panaxytriol 함량 분석값은 각각 724, 721 과 71$\mu\textrm{g}$/g이었다.

• 약학회지
• /
• 제33권2호
• /
• pp.118-123
• /
• 1989

A new polyacetylene compound which has strong cytotoxic activity against L1210 cell, was isolated from Korean ginseng roots. The structure was determined to be heptadeca-1-ene-4,6-diyne-3,9-diol-10-acetate (10-acetyl panaxytriol, $ED_{50}\;=\;1.2\;{\mu}g/ml$). The cytotoxicities of this compound and acetyl panaxydol lower than their starting substances, panaxytriol and panaxydol. The presence of one for the decreases in the cytotoxicities.

• Archives of Pharmacal Research
• /
• 제24권4호
• /
• pp.297-299
• /
• 2001

This study investigated the effect that some polyacetylenes and protopanaxatriol, which were isolated from heated ginseng (family Araliaceae), have on inhibiting Helicobacter pylori (HP) growth. Among the compounds tested, panaxytriol was quite effective in inhibiting HP growth with an MIC of 50 ${\mu}g/ml$. Cinsenoside Rhl and protopanaxatriol weakly inhibited $H^{+}/K^{+}$-ATPase from a rat stomach.

• Toxicological Research
• /
• 제6권1호
• /
• pp.13-19
• /
• 1990

The effects of panaxytrion as known to be a cytotoxic substance isolated from Panax ginseng on the immune responses were examined. The i.p. administration of panaxytriol to normal mice for 6 consecutive days as doses of 5, 10 and 20 mg/kg suppressed the increase of body weight dose-dependently but did not affect the weight ratio of immunoorgans to body weight, No significant changes were observed in the humoral immune responses as measured by Arthus reaction and plaque forming cells and in the cellular immune response as measured by delayed hypersensitivity as well as phagocytic activity of reticuloendotherial system. These results suggested that panaxytriol, a cytotoxic substance to cancer cells, has no detrimental effects on the immune function in normal mice.

• Archives of Pharmacal Research
• /
• 제12권3호
• /
• pp.207-213
• /
• 1989

This study was performed to investigate the mechanism of in vitro cytotosic actions of polyacetylenes which are panaxydol, panaxynol and panaxytriol isolated from Panax ginseng C. A. Meyer. DNA synthesis of L1210 cells was significantly inhibited with dose dependent pattern when L1210 cells were treated for 1 hour with over 5 .mu.g/ml of polyacetylenes. Panaxydol which had the most potent cytotoxicity among three polyacetylenes showed also the strongest inhibitory effect on DNA synthesis. Intracellular cyclic AMP levels of L1210 cells treated with 2.5 $\mu$g/ml of panaxydol or panaxytriol were significantly elevated on the incubation duration. The elevation of cyclic AMP levels by panaxytriol was higher than that by panaxydol, but no significant increase in cyclic AMP by panaxynol was observed. All three polyacetylenes had no effect on glycolysis of L1210 cells. Electron microscopic observations revealed that polyacetylenes caused damage to plasma membranes of L1210 cells in proportion to their cytotoxicities at each $ED_{50}$ value (panaxydol > panaxynol> panaxytriol). These results suggest that cytotoxicities of polyacetylenes against L1210 cells might be mediated by elevated cyclic AMP level, even though the relationship among their cytotoxicities, inhibitory effect on DNA synthesis and ability to elevation of cyclic AMP level are not fully agreed, and might be also related to membrane damage.

• Journal of Ginseng Research
• /
• 제15권1호
• /
• pp.13-20
• /
• 1991

Ginseng components were examined for their ability to modify immunotoxicity of cyclophosphamide. Ginseng polysaccharide fraction (FO, 300 mg/kg) inhibited decreases in the ratio of spleen to body weight, white blood cells (WBC) count and the number of plaque forming cells (PFC) induced by cyclophosphamide (50 mg/kg, i.p.), and increased these variables in normal mice. Ginseng saponin fraction (50 mg/kg) showed to increase hemoglobin content as well as the number of PFC/spleen in normal mice. on the other hand, Panaxytriol (20 mg/kg) Prevented decrease in mc count by cyclophos phamide. Neither saponin fraction nor panaxytriol had any significant effect on the number of PFC and antibody titers in cyclophosphamide-treated mice. These results suggest that ginseng polysaccharine fraction may reduce the immunotoxicity of cyclophosphamide and may be effective in stimulating immune function in normal mice.

• Journal of Ginseng Research
• /
• 제20권1호
• /
• pp.30-35
• /
• 1996

Some polyacetylenes were Isolated from the rhizome of Korean red ginseng through re - pealed column chromatogrphy, and identified as 3-hydroxy-1, 9-heptadecadiene-4, 6-diyne (panaxynol), 9, 10-epoxy-3-hydroxy-1-heptadecene-4, 6-diane(panaxydol), 3, 9, 10-trihydroxy-1-heptadecene-4, 6-diyne(panaxytriol), 9, 10-epoxy-3-hydroxy-1, 16-heptadecadiene-4, 6-diyne(ginsenoyne A) and 10-ch1oro-3, 9-dihydroxy-1-heptadecene-4, 6-diane(panaxydol chlorohydrine) by several spectral and chemical methods.