• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.574-581
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• 2011

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.939-945
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• 2003

Seventeen Chinese indigenous pig breeds and three introduced pig breeds had been carried out by means of vertical polyacrylamide gel electrophoresis (PAGE). According to the results, eight serum protein loci were highly polymorphic except Pi-2 and Cp. The polymorphism information content (PIC) of Hpx was the highest (0.5268), while that of Cp was the lowest (0.0257). The population genetic variation index showed that about 84% genetic variation existed in the population, and the rest of 16% distributed between the populations. The genetic variation of Yimeng black pig and Duroc were the highest and the lowest, respectively. The genetic variation of Chinese indigenous pig breeds was much more than that of exotic groups. Genetic distance results showed that Chinese indigenous pig breeds were classified into four groups with the three introduced pig breeds clustered into another group. The results also supported the geographic distribution of Chinese indigenous pig breeds in certain extent.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.351-359
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• 2014

For identifying the plasmid DNA coding cry gene of Bacillus thuringiensis subsp. aizawai KB098 with high insecticidal activity against Spodoptera exigua, mutant isolates with no crystal protein were produced by $42^{\circ}C$ incubation condition and then mutant plasmid DNA band patterns were compared with those of KB098. KB098 isolates had 4 cry genes, cry1Aa, cry1Ab, cry1C, cry1D, and also had been found seven plasmid DNA. Though the SDS-PAGE experiment, it was confirmed that mutant didn't produce 130~145kDa protein band involved in bipyramidal shape crystal. Also, five mutant isolates had no cry genes coding plasmid DNA in PCR. In result of comparison the plasmid DNA of KB098 and 5 mutant isolates, only 1 plasmid DNA band was left out in mutant plasmid DNA pattern, so that the missing band was extracted from the gel. The missing(disappeared) plasmid DNA was the largest molecular size among the 7 plasmid DNA of KB098 and it was also confirmed this plasmid DNA had all 4 cry genes through PCR.

• Journal of KIISE
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• v.44 no.2
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• pp.132-138
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• 2017

We design and implement a process-based fault recovery system to increase the reliability of new memory based computer systems. A rollback point is made at every context switch to which a process can rollback to upon a fault. In this study, a clone process of the original process, which we refer to as a P-process (Persistent-process), is created as a rollback point. Such a design minimizes losses when a fault does occur. Specifically, first, execution loss can be minimized as rollback points are created only at context switches, which bounds the lost execution. Second, as we make use of the COW (Copy-On-Write）mechanism, only those parts of the process memory state that are modified (in page units) are copied decreasing the overhead for creating the P-process. Our experimental results show that the overhead is approximately 5% in 8 out of 11 PARSEC benchmark workloads when P-process is created at every context switch time. Even for workloads that result in considerable overhead, we show that this overhead can be reduced by increasing the P-process generation interval.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.3 no.5
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• pp.548-574
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• 2009

Personal information (hereinafter referred to as "PI") infringement has recently emerged as a serious social problem in Korea. PI infringement in the public and private sector is common. There were 182,666 cases of PI in 2,624 public organizations during the last three years. Online infringement cases have increased. PI leakage causes moral and economic damage and is an impediment to public confidence in public organizations seeking to manage e-government and maintain open and aboveboard administration. Thus, it is an important matter. Most cases of PI leakage result from unsatisfactory management of security, errors in home page design and insufficient system protection management. Protection management, such as encryption or management of access logs should be reinforced urgently. However, it is difficult to comprehend the scope of practical technology management satisfied legislation and regulations. Substantial protective countermeasures, such as access control, certification, log management and encryption need to be established. It is hard to deal with the massive leakage of PI and its security management. Therefore, in this study, we analyzed the conditions for the technical protection measures during the processing phase of PI. In addition, we classified the standard control items of protective measures suited to public circumstances. Therefore, this study provides a standard and checklist by which staff in public organizations can protect PI via technical management activities appropriate to laws and ordinances. In addition, this can lead to more detailed and clearer instructions on how to carry out technical protection measures and to evaluate the current status.

• Archives of design research
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• v.13 no.3
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• pp.111-122
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• 2000

To achieve the advertising objectives, we must first draw out consumer's advertisingto advertising, and then realize the data processing course from comprehension to the retention of the advertising contents. In current media environment, however, the value of advertising as the method of attracting consumer's attention is declining due to the current situation of newspaper and magazine advertising as follows. As for newspapers, competitive messages are increasing on a continuous basis; the size of advertising section and the number of newspaper pages has been increased along with the popularization of color advertising. As for magazines, more pages are allocated for advertising rather than for the actual contents. In such communication environment, it is believed that only the advertising capable of uniquely appealing to consumers can achieve the purpose of advertising. Hence, this research attempts to discuss the effective ways of promoting attention in print media through the case studies covering the design principles relating to layout and the layout format, among the visualization processes related to the consumers'memory.

• Journal of Korean Library and Information Science Society
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• v.35 no.4
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• pp.361-375
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• 2004

This study was to predict through the scenarios, one of the forecast technique the future of library service. In order to do that literature reviews, analysis of library's home page and specialist interview were conducted. As a result, it appeared that based on the scenarios to predict the middle future(after 5-10 years) of library service. The results of this study are as follows. The first the future information environment is represented by mobile md ubiquitous. Through this, anytime, anywhere, any device, we can be taken any media The second, the unreliable factors come out in time lag, the supply speed of terminals, library services, copyrights and so on. The third, as the supply speed of technique and the speed of technological development, the speed of technological development and the solution speed of digital copyright problem these make various scenarios. The fourth library try to find unreliable factors in constant change of the information technique, variety of internal factors. And strategy needs to dispose of various scenarios properly.

• Journal of the Korean Society of Costume
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• v.51 no.3
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• pp.129-139
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• 2001

The purpose of this study is to suggest the standard size specification of the apparel item as finding out apparel products'sizing problem in the domestic and foreign interned shopping malls. Though that, to suggest basic data of web page offered sizing system given to consumer higher satisfaction and based on ISO (International Organization For Standardization), domestic industry standard and service related size. The summarized findings are as follow. 1. The result of size description research, the size description system between internet site were very different, in addition in the department shopping malls it was different by manufacturing industry. Also in domestic and foreign shopping malls, It was not correspond to ISO and domestic industry standard, used Numeric and Alphabetical size. In the foreign shopping mall it distinguish body type and age group. 2. The result of consumer's satisfaction, the superior fashion malls were Samsung, LG, Fashionpia in the domestic shopping mall, were Gap, JCPenny, Jcrew in the foreign shopping malls. In the total group, 1-5 grades were ranked in the foreign shopping malls. 3. Satisfaction of the foreign fashion mall was higher than that of the domestic fashion malls. Exactly, foreign fashion mall described size chart, size description, product size and body size measuring method but domestic fashion malls didn't describe that. Domestic fashion malls need sizing system development given to consumer higher satisfaction. In addition sizing system based on ISO and domestic industry standard and subdivided to body type and ale group must be studied. Aloe, fashion information, coordination information and product explanation must be reinforced.

• KSBB Journal
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• v.26 no.3
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• pp.199-205
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• 2011

Using tetrameric ${\beta}$-galactosidase as a model protein, anchoring motives were screened in Bacillus subtilis spore display system. Eleven spore coat proteins were selected considering their expression levels and the location in the spore coat layer. After chromosomal single-copy homologous integration in the amyE site of Bacillus subtilis chromosome, cotE and cotG were chosen as possible spore surface anchoring motives with their higher whole cell ${\beta}$-galactosidase activity. PAGE and Wester blot of extracted fraction of outer layer of purified spore, which express CotE-LacZ or CotG-LacZ fusion verified the existence of exact size of fusion protein and its location in outer coat layer of purified spore. ${\beta}$-galactosidase activity of spore with CotE-LacZ or CotG-LacZ fusion reached its highest value around 16~20 h of culture time in terms of whole cell and purified spore. After intensive spore purification with lysozyme treatment and renografin treatment, spore of BJH135, which expresses CotE-LacZ, retained only 1~2% of its whole cell ${\beta}$-galactosidase activity. Whereas spore of BJH136, which has cotG-lacZ cassette in the chromosome, retained 10~15% of its whole cell ${\beta}$-galactosidase activity, proving minor perturbation of CotG-LacZ, when incorporated in the spore coat layer of Bacillus subtilis compared to CotE-LacZ. Usage of Bacillus subtilis WB700, of which 7 proteases are knocked-out and thereby resulting in 99.7% decrease in protease activity of the host, did not prevent the proteolytic degradation of spore surface expressed CotG-LacZ fusion protein.

• BMB Reports
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• v.32 no.3
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• pp.239-246
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• 1999

A tripeptidase (PepT) was purified to homogeneity from Bacillus subtilis through four sequential chromatographies including DEAE-Sepharose ion exchange, hydroxylapatite, mono-Q FPLC ion exchange, and Superose-12 FPLC gel filtration. The apparent molecular mass of the enzyme was 49,200 Da and 51,400 Da as determined by sodium dodecylsulfatepolyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography, respectively, and the enzyme exists in a monomeric form. The physicochemical properties of the enzyme were as follows: optimum pH at 7.5, optimum temperature at $60^{\circ}C$, and pI at 4.9. The $K_m$ and $V_{max}$ values of the enzyme were 4.3 mM and 2.5 mmol/min/mg, respectively, with MetAla-Ser as substrate. The B. subtilis PepT requires $Co^{2+}$ ion(s) for activation, while it is inactivated by EOTA and 1,10-phenanthroline, suggesting that it is a metalloprotein. The enzyme was not inhibited by any of serine protease, aspartic protease, or leucine aminopeptidase inhibitors. The enzyme showed comparable activities towards four different substrates including Met-Ala-Ser, Leu-Gly-Gly, Leu-Ser-Phe, and Leu-Leu-Tyr. The amino terminal sequence of PepT determined by Edman degradation was found to be MKEEIIERFTTYVXV and turned out to be identical to that of PepT deduced from a cloned B. subtilis pepT.