• Parasites, Hosts and Diseases
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• v.30 no.2
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• pp.91-100
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• 1992

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf values, strips of gel containing each band protein was cut out. Each band protein was eluted by electrophoresis. Monospecific antibodies were prepared by immunizing rabbits with each band protein. When peroxidase-antiperoxidase (PAP) staining was done, antiserum to Band 1 reacted to content of eggs both in the worm and in the infected lung tissue. Antiserum to Band 2 reacted to parenchymal tissue of the worm. Antiserum to Band 4 showed the positive reaction at intestinal content while that to Band 5 reacted to the intestinal epithelial border. Antiserum to combined proteins of Bands 617 and that to Band 8 reacted to parenchymal tissue of the worm respectively. From the results, the origin of individual proteins in crude extract of adult P. westermani could be differentiated.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.12
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• pp.1516-1528
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• 1999

월드 와이드 웹(World Wide Web)에 존재하는 음란성 유해 정보는 많은 국가에서 사회적인 문제를 일으키고 있다. 그러나 현재 음란성 유해 정보로부터 미성년자를 보호하는 실효성 있는 방법은 유해 정보 접근 차단 프로그램을 사용하는 방법뿐이다. 유해 정보 접근 차단 프로그램은 기본적으로 음란성 유해 정보를 포함한 유해 정보 주소 목록을 기반으로 사용자의 유해 정보에 대한 접근을 차단하는 방식으로 동작한다.그런데 대규모 유해 정보 주소 목록의 확보를 위해서는 월드 와이드 웹으로부터 음란성 유해 정보를 자동 색출하는 인터넷 정보 검색 시스템의 일종인 음란성 유해 정보 색출 시스템이 필요하다. 그런데 음란성 유해 정보 색출 시스템은 그 대상이 사람이 아닌 유해 정보 접근 차단 프로그램이기 때문에 일반 인터넷 정보 검색 시스템과는 달리, 대단히 높은 검색 정확성을 유지해야 하고, 유해 정보 접근 차단 프로그램에서 관리가 용이한 검색 목록을 생성해야 하는 요구 사항을 가진다.본 논문에서는 기존 인터넷 정보 검색 모델이 "문헌"에 대한 잘못된 가정 때문에 위 요구사항을 만족시키지 못하고 있음을 지적하고, 월드 와이드 웹 상의 문헌에 대한 새로운 정의와 이를 기반으로 위의 요구사항을 만족하는 검색 모델인 페이지 그룹 검색 모델을 제안한다. 또한 다양한 실험과 분석을 통해 제안하는 모델이 기존 인터넷 정보 검색 모델보다 높은 정확성과 빠른 검색 속도, 그리고 유해 정보 접근 차단 프로그램에서의 관리가 용이한 검색 목록을 생성함을 보인다.Abstract Illegal and Harmful Content on the Internet, especially content for adults causes a social problem in many countries. To protect children from harmful content, A filtering software, which blocks user's access to harmful content based on a blocking list, and harmful content search system, which is a special purpose internet search system to generate the blocking list, are necessary. We found that current internet search models do not satisfy the requirements of the harmful content search system: high accuracy in document analysis, fast search time, and low overhead in the filtering software.In this paper we point out these problems are caused by a mistake in a document definition of the current internet models and propose a new internet search model, Page Group Search Model. This model considers a document as a set of pages that are made for one subject. We suggest a Group Construction algorithm and a Group Evaluation algorithm. And we perform experiments to prove that Page Group Search Model satisfies the requirements.uirements.

• Biomedical Science Letters
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• v.6 no.3
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• pp.201-208
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• 2000

The vancomycin, one of the family of glycopeptide antibiotics, inhibits the synthesis of bacterial cell wall peptidoglycan and has been widely used against gram-positive bacterial infections, especially for a treatment of methicillin resistant S. aureus infection. However, clinical isolate which was intermediately resistant to vancomycin (Mu50: MIC 8 $\mu\textrm{g}$/ml) was isolated in recent years. In this study we performed vancomycin susceptibility test with the increment method and population analysis with clinical isolates S. aureus. Also we did several kinds of tests with three selected isolates (s129: MIC 7 $\mu\textrm{g}$/ml, s134: MIC 7 $\mu\textrm{g}$/ml, s135: MIC 8 $\mu\textrm{g}$/ml) to find out possible mechanism of vancomycin resistance. As a result, the prevalence of vancomycin resistant S. aureus isolates among S. aureus strains resistant to methicillin was 23.3% (25/107). The vancomycin resistances of isolated strains of S. aureus were between those of Mu5O and Mu3 strains. By PCR analysis, none of the isolates with decreased vancomycin susceptibility contained known vancomycin resistant genes such as vanA, vanB, vanC1, vanC2, and vanH. Major bands of 81 kDa, 58 kDa, 33 kDa, 28 kDa were demonstrable in whole cell lysates by SDS-PAGE from all three isolates as well as reference strains. And especially,45 kDa protein was overproduced in Mu50 strains. Among them increased production of NAD$^{+}$-linked-$_{D}$-lactate dehydrogenase (dnLDH) were detected from one clinical strain (s135) and Mu5O strain. From these data, we suggest that the mechanism of vancomycin resistance in these isolates are distinct from that in enterococci.

• Journal of the Korean Association of Geographic Information Studies
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• v.5 no.4
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• pp.93-105
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• 2002

In this paper forest fire status information management system was developed under web environment using web GIS(geographic information system) technology. Though this system, general users can easily retrieval domestic forest fire status information and obtain that in visual way such as maps, graphs, and texts if they have only certain web browsers. Moreover, officials, who have system access authority, can easily control and manage all domestic forest fire status information through input interface, retrieval interface, and out interface of the system. This system can be considered as the first domestic system to manage forest fire status data and service them in visual through user friendly interfaces on web. In order to implement this system, IIS 5.0 of Microsoft is used as web server and Oracle 8i and ASP(active server page) are used for database construction and dynamic web page operation, respectively. Also, ArcGIS IMS(internet map server) of ESRI is used to serve maps by using Java and HTML as system development languages. Not only the domestic tendency of forest fire but also the forest fire status information of certain area and time such as the frequency and the loss can be presented through distribution maps, graphs and tables. Therefore, this system is supposed to play as a important role when the policy relate to domestic forest fire is established. In addition, the self consciousness of people against forest fire can be inspired and the foundation of scientific and systemic forest fire services can be obtained through this system in the future.

• Korean Journal of Agricultural Science
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• v.22 no.1
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• pp.82-95
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• 1995

These experiments were carried out to isolate SIgA from human and bovine milk. The immunochemical properties of SIgA from human and bovine milk were examined by Gel filtration, DEAE and SDS-PAGE. Double Immunodiffusion, and Immunoelectrophoresis. The results obtained are as follows: 1. Human SIgA was purified from colostrum of Korean women by repeated gel filtration on Sephadex G-200 and Sepharose 6B, but bovine SigA was not cleary purified from bovine colostrum of Holstein cows by anion exchange chromatography using DEAE-Sephadex A-50 and gel filtration on Sephadex G-200 and Sepharose 6B. 2. The immunochemical properties of fractions from gel filtration on the Sephadex G-200 and Sepharose 6B column as assessed by Immunoelectrophoresis and double Immunodiffusion to identify the presence of IgM in first peak fraction, and the presence of pure SIgA in second peak fraction. However, Bovine SigA rich fraction from bovine colostrum of Holstein cows contained a large amount of $IgG_1$-dimer in addition to SIgA. 3. The fragments of reduced bovine colostrum SIgA rich fraction were estimated to have molecular weights of secretory component, heavy chain and light chain (75,000-80,000, 50,000-60,000, 25,000-27,000 daltons) by SDS-PAGE, respectively. Those were similar to the molecular weight of reduced SIgA from human milk.

• Communications of Mathematical Education
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• v.31 no.1
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• pp.17-48
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• 2017

This case study examined mathematics anxiety of a public high school sophomore who was unable to perform well in mathematics but later overcame his fear of mathematics. In this study, he showed high levels of mathematics anxiety in the assessment tools that evaluate mathematical anxiety factors. Cognitive and behavior treatments were carried out to alleviate his anxiety. First, cognitive treatments that were implemented include: understanding his own problems, writing down his thoughts on a record sheet, and changing intermediate and core beliefs. This paper explored cognitive and affective changes and reactions during the treatment process. Second, behavioral treatments that were conducted include: the divided-page method and peer tutoring. The divided-page technique involves the test subject to write down and solve his problems on a note to see what kind of cognitive and affective changes occur during the process. This paper also explored how Su-chul, an overly competitive student, changed and reacted cognitively and affectively through peer tutoring. The results revealed that Su-chul's exam anxiety, as well as other factors, has decreased. Moreover, he regained his self-confidence by solving math problems that he had felt difficult. His competitive attitude also has turned into a cooperative and thoughtful one.

• Journal of the Korea Society of Computer and Information
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• v.12 no.6
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• pp.251-259
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• 2007

A Bluetooth ad hoc network can be formed by interconnecting piconets into scatternet. Many algorithms of scatternet formation have been proposed so far. However, these have a problem that takes a long time to reform scatternet, especially for the case of master moving out. In this paper we propose a Fast-Recovery algorithm. which aims at reducing the time of scatternet reformation owing to master moving out. In the algorithm, we select a slave with the weigh similar to its master as Sub-Master from a piconet device table created by the master When a master leaves its piconet is detected by its slaves, the Sub-Master becomes the new master of the piconet instead of the old and directly connect to other slaves and bridge(s) through piconet device table in page state.

• International Commerce and Information Review
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• v.6 no.2
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• pp.219-242
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• 2004

This study is conducted to find out whether companies which use electronic commerce for their business are collecting the least personal information. of consumers that are necessary for providing electronic commerce services to consumers. Investigators visited website of 799 electronic commerce companies, and checked out the personal informations of consumers that were asked when they join the site as a member. The collected data were analyzed with frequency, percentage, and cross-tab analysis using SPSS program. Generally most electronic commerces required more personal information of consumers than necessary for providing their services to consumers. These phenomena are partly due to the fact that regulations regarding consumer privacy in electronic commerce are advisory rather than mandatory at present. Therefore, it is suggested that mandatory regulation which makes companies poot their identification within a certain area of their web page has to enforced for the protection of consumer privacy in electronic commerce. And it would be more efficient if consumers are consistently educated about self protection guideline regarding personal information in using electronic commerce in addition to a mandatory regulation.

• Journal of the Korean Society of Food Culture
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• v.19 no.1
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• pp.83-93
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• 2004

A total of 321 salaried employees were surveyed by a questionnaire to assess consumers perceptions on factors influencing their food choices when they go to a restaurant. The questionnaire consisted of 5 pages and each page contained a same 26 popular food list from various restaurants in JinJu in a column and 5 attributes (e.g. taste, price, fullness, healthiness) in a row. So the respondents were asked to rate a total 25 attributes on the points which the columns and the rows met, using a 5-point scale. The data were analyzed using the SAS program for factor analysis. Five factors which influence consumer's restaurant choice were emerged. They were named as follow: Factor 1:preference and purpose, Factor 2:taste, Factor 3:snack, Factor 4:convenience, Factor 5:concern about obesity. Most of respondents were satisfied with the taste and the healthiness of foods of restaurants in Jin-Ju. The younger and the female groups had more positive attitudes on eating out andi willing to use it frequently. Fullnes, healthiness, and price were important criteria in choice and preference of a restaurant. However, they didn't much consider the food safety. Taking account of the character of Korean food about fullness, healthiness, and resonable price, consumers might prefer Korean food to fast food or foreign food.

• Korean Journal of Agricultural Science
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• v.25 no.1
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• pp.52-67
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• 1998

The investigative research on the mammalian milk purely consisted of the physiological quality of lactoferrin was conducted to reveal the antimicrobial ativity of specifically functional foods with antibiotic characteristics as a basic data in food manufacturing. Bovine lactoferrin were isolated from raw milk samples, and was digested with pepsin, trypsin and chymotrypsin. It was necessary then to separate and purify lactoferrin from bovine raw milk, and in order to analyze the antimicrobial activity of the enzyme-treated bovine lactoferrin in their required quantitative fraction. Afterwards Escherichia coli and Staphylococcus aureus was incubated in it. It was that investigated to enzyme-treated fractions molecular weight and the peptide fragment with antimicrobial effect. 1. The purity of enzyme-treated bovine lactoferrin(BLF) was tested by SDS-PAGE. As a results of 12% SDS-PAGE assay, pepsin-treated LF did not exhibited band until if reaches 14 KDa, while trypsin and chymotrypsin treated LF, known to contain the non-digestive lactoferrin exhibited band at a molecular weight of 33 KDa. 2. Bovine lactoferrin was sucessfully purified through the use of Sephadex G-50 Column. In order to assay LF through the Sephadex G-50 column chromatography, the digestive bovine lactoferrin (BLFs) was eluted with a linear gradient of 0.05% Tris-HCl. When the gel-filtration analysis, pepsin, trypsin and chymotrypsin treatments of BLF fragments was showed 2, 3, and 2 peak, respectively. The results of the HPLC analysis confirmed that had a non-digestive lactoferrin receptor, and trypsin and chymotrypsin treated BLFs has an antimicrobial effect. 3. To measure the strength of the antimicrobial effect of enzyme treated lactoferrin it was compared to the antimicrobial activity taking place at the incubated Escherichia coli and Staphylococcus aureus. This might explain the resistance of the microorganisms for peptide fragment. The pepsin-treated of bovine lactoferrin was markedly reduced by incubation of the cells. Trypsin-treated of BLF was similar to chymotrypsin-treated of BLF. However, trypsin and chymotrypsin treatments of BLFs were showed the antimicrobial effect until eight hours incubation for native bovine lactoferrin. Therefore the enzyme-treated lactoferrin have an antimicrobial effect even non-digestive lactoferrin. 4. The digestive bovine lactoferrin fragments assay was carried out by the use of Sephadex G-50 column chromatography and SDS-PAGE. The pepsin and chymotrypsin-treated fragments has a low molecular weight and trypsin-treated lactoferrin was only showed a band. It was described that characteristics of digestive protein. It appeared that there may be a relation between virulence and resistance to enzyme-treated BLF.