• 대한화학회지
• /
• 제53권4호
• /
• pp.407-414
• /
• 2009

o-phenylenediamine, 3-acetyl-6-methyl-(2H) pyran, 2,4 (3H)-dione (dehydroacetic acid or DHA) 및 살리실 알데히드로부터 유도한 4-hydroxy-3-(1-{2-(2-hydroxy-benzylidene)-amino phenylimino}-ethyl)-6- methy-pyran-2-one (H2L) 의 Cu(II), Ni(II), Co(II), Mn(II) 및 Fe(III) 고체 착물을 합성한 후, 원소분석, 전도 도법, 수자율, 자외선-가시선, 적외선, $^1H$-NMR 스펙트라, X-선 회절, 열분석을 통해 특성을 규명하고 항균 활성을 조사하였다. IR 스펙트럼 데이터로부터 이 리간드가 중심 금속이온에 대해 ONNO 주개원자 배열을 갖는 이염기성 네자리 리간드로 행동함을 제안하였다. 원소분석 데이터로부터 이들 착물의 화학량론이 1:1 (금속:리간드)임을 알았다. 물리-화학적 데이타로부터 Cu(II) 및 Ni(II) 착물이 사각평면 기 하구조, 그리고 Co(II), Mn(II) 및 Fe(III) 착물이 팔면체 기하구조임을 제안하였다. X-선 회절 데이터로 부터 Cu(II) 착물이 사방정계(orthorhombic) 결정계, Ni(II), Co(II) 및 Fe(III) 착물이 단사계(monoclinic) 결정계 그리고 Mn(II) 착물이 정방정계(tetragonal) 결정계임을 제안하였다. 착물의 열적 행동(TG/DTA) 을 연구하였으며 Coats-Redfern 방법으로 반응속도 파라메터를 결정하였다. 리간드와 이들 금속 착물을 이용하여 Staphylococcus aureus 및 Escherichia coli에 대한 향균 활성과 Aspergillus Niger 및 Trichoderma 에 대한 살균 활성을 조사하였다.

• Bulletin of the Korean Chemical Society
• /
• 제27권10호
• /
• pp.1593-1596
• /
• 2006

The polymeric membrane electrodes based on N,N'-bis-pyridin-2-ylmethylene-naphthalene-1,8-diamine as an ion carrier were prepared and tested for the copper-ion selective electrode. The membrane has a linear dynamic range between $10^{-6}$ and $10^{-2}$ M with a Nernstian slope of 29.6 mV per decade, and its detection limit was $10^{-5.62}$M. The potentiometric response is independent of the pH range of 3-5. The proposed electrode showed good selectivity and response for $Cu^{2+}$ over a wide variety of other metal ions in pH 4.0 buffer solutions.

• Bulletin of the Korean Chemical Society
• /
• 제31권4호
• /
• pp.881-886
• /
• 2010

A novel Schiff base N-(2,4-dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine has been synthesized and structurally characterized by X-ray single crystal diffraction, elemental analysis, IR spectra and UV-vis spectrum. The crystal belongs to monoclinic with space group P21/n. The molecules are connected via intermolecular O-$H{\cdots}O$ hydrogen bonds into 1D infinite chains. The crystal structure is consolidated by the intramolecular N-$H{\cdots}O$ hydrogen bonds. weak intermolecular C-$H{\cdots}O$ hydrogen bonds link the molecules into intriguing 3D framework. Furthermore, Density functional theory (DFT) calculations of the structure, stabilities, orbital energies, composition characteristics of some frontier molecular orbitals and Mulliken charge distributions of the title compound were performed by means of Gaussian 03W package and taking B3LYP/6-31G(d) basis set. The time-dependent DFT calculations have been employed to calculate the electronic spectrum of the title compound, and the UV-vis spectra has been discussed on this basis. The results show that DFT method at B3LYP/6-31G(d) level can well reproduce the structure of the title compound.

• 대한화학회지
• /
• 제54권5호
• /
• pp.515-522
• /
• 2010

o-phenylene diamines, dehydroacetic acid (DHA) 및 p-chloro benzaldehyde에서 유도된 세자리 Schiff 염기 리간드인 4-hydroxy-3(1-{2-(benzylideneamino)-phenylimino}-ethyl)-6-methyl-2H-pyran-2-one (HL)의 Cu(II), Ni(II), Co(II), Mn(II) 및 Fe(III) 착물의 형성상수와 항미생물 활성과의 관계를 연구하였다.리간드와 착물은 원소분석, 전도도, 자기수자율, 열분석, X-선 회절, IR, $^1H$-NMR, UV-vis 및 질량 스펙트럼으로 특성조사를 하였다. 분석데이터로부터 착물들의 화학량론비가 1:2 (금속:리간드)임을 알았다. 금속 착물들의 몰 전도도 값은 이들의 비전해질 성질을 의미한다. X-선 회절 데이터에서 Ni(II) 착물은 단사정계 그리고 Cu(II) 및 Co(II) 착물은 삼사정계 결정계임을 규명하였다. IR 스펙트럼 데이터로부터 리간드는 중심금속에 대해 ONN 주개원자 배열의 세자리 리간드로 행동함을 알았다. 열적 행동 (TG/DTA)과 Coats-Redfern 법에 의해 계산한 반응속도 파라메터는 착물형성 과정에서 좀 더 질서 있는 활성화 상태를 제안하고 있다. 착물의 양성자화 상수를 THF:물 (60:40) 용액, $25^{\circ}C$ 및 이온세기 ${\mu}=0.1\;M$ ($NaClO_4$)에서 전위차법으로 측정하였다. Staphylococcus aureu 및 Escherichia coli.에 대한 항박테리아 활성을 시험관에서 조사하였다. 또한 Aspergillus Niger 및 Trichoderma에 대한 항세균 활성도 조사하였다. 금속 이온 및 착물의 안정도가 항미생물학적 활성에 미치는 영향을 고찰하였다.

• Mycobiology
• /
• 제40권1호
• /
• pp.20-26
• /
• 2012

The complexes of tailor made ligands with life essential metal ions may be an emerging area to answer the problems of multi drug resistance. The coordination complexes of VO(II), Co(II), Ni(II) and Cu(II) with the Schiff bases derived from isatin with 3-chloro-4-floroaniline and 2-pyridinecarboxaldehyde with 4-aminoantipyrine have been synthesized by conventional as well as microwave methods. These compounds have been characterized by elemental analysis, molar conductance, electronic spectra, FT-IR, FAB mass and magnetic susceptibility measurements. FAB mass data show degradation of complexes. Both the ligands behave as bidentate and tridentate coordinating through O and N donor. The complexes exhibit coordination number 4, 5 or 6. The Schiff base and metal complexes show a good activity against the bacteria; $Staphylococcus$ $aureus$, $Escherichia$ $coli$ and $Streptococcus$ $fecalis$ and fungi $Aspergillus$ $niger$, $Trichoderma$ $polysporum$, $Candida$ $albicans$ and $Aspergillus$ $flavus$. The antimicrobial results also indicate that the metal complexes are better antimicrobial agents as compared to the Schiff bases. The minimum inhibitory concentrations of the metal complexes were found in the range 10-40 ${\mu}g/mL$.

• Journal of Microbiology and Biotechnology
• /
• 제28권8호
• /
• pp.1339-1345
• /
• 2018

2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase, which catalyzes aldol cleavage and condensation reactions, has two distinct substrate-binding sites. The substrate-binding mode at the catalytic site and Schiff-base formation have been well studied. However, structural information on the phosphate-binding loop (P-loop) is limited. Zymomonas mobilis KDPG aldolase is one of the aldolases with a wide substrate spectrum. Its structure in complex with the substrate-mimicking 3-phosphoglycerate (3PG) shows that the phosphate moiety of 3PG interacts with the P-loop and a nearby conserved serine residue. 3PG-binding to the P-loop replaces water molecules aligned from the P-loop to the catalytic site, as observed in the apostructure. The extra electron density near the P-loop and comparison with other aldolases suggest the diversity and flexibility of the serine-containing loop among KDPG aldolases. These structural data may help to understand the substrate-binding mode and the broad substrate specificity of the Zymomonas KDPG aldolase.

• 통합자연과학논문집
• /
• 제9권4호
• /
• pp.255-260
• /
• 2016

The crystal structure of the saliciline derivatives N,N'-bis(3-chloro-2-methylsalicylidene)-1,4-butanediamine ($C_{20}H_{22}Cl_2N_2O_2$) has been determined from single crystal X-ray diffraction data. In the title compound crystallizes in the triclinic space group $P{\bar{i}}$ with unit cell dimension $a=4.6085(3){\AA}$, $b=5.9747(3){\AA}$ and $c=5.9747(3){\AA}$ [${\alpha}=83.889(4)^{\circ}$, ${\beta}=86.744(5)^{\circ}$ and ${\gamma}=82.085(5)^{\circ}$]. The title compound is essentially planar conformation. The compound lies across a crystallographic inversion centre and adopts E configurations with respect to the C-N bonds. The crystal packing of the molecules of compound is stabilized through weak O-H...N intra molecular interactions

• 통합자연과학논문집
• /
• 제9권4호
• /
• pp.261-267
• /
• 2016

The crystal structure of the saliciline derivatives 4-chloro-2{[(2-hydroxy-5-methylphenyl)amino]methyl}5-methylphenol ($C_{15}H_{15}ClNO_2$) has been determined from single crystal X-ray diffraction data. In the title compound crystallizes in the monoclinic space group P21/c with unit cell dimension $a=11.5241(2){\AA}$, $b=8.733(2){\AA}$ and $c=13.649(2){\AA}$ [${\alpha}=90^{\circ}$, ${\beta}=130.876(2)^{\circ}$ and ${\gamma}=90^{\circ}$]. the title compound are essentially planar conformation. The compound lies across a crystallographic inversion centre and adopts E configurations with respect to the C-N bonds. The crystal packing of the molecules of compound is stabilized through weak O-H...O inter molecular interactions.

• 약학회지
• /
• 제37권3호
• /
• pp.247-253
• /
• 1993

Eight new derivatives of allylamine which are related to naftifine and expected to have antifungal activity were synthesized. Schiff base 2a-2d and 2'e-2'h were obtained by conventional methods using trans-cinnamylamine or trans-cinnamaldehyde. The reduction of azomethine linkage with NaBH$_{4}$ yielded secondary amine 3a-3h, which were then methylated by means of HCHO and excess NaBH$_{4}$ or HCOOH to give 4a-4h.

• Journal of Microbiology and Biotechnology
• /
• 제26권5호
• /
• pp.829-836
• /
• 2016

Penicillin G acylase (PGA) was immobilized on magnetic Fe₃O₄@chitosan nanoparticles through the Schiff base reaction. The immobilization conditions were optimized as follows: enzyme/support 8.8 mg/g, pH 6.0, time 40 min, and temperature 25 ℃. Under these conditions, a high immobilization efficiency of 75% and a protein loading of 6.2 mg/g-support were obtained. Broader working pH and higher thermostability were achieved by the immobilization. In addition, the immobilized PGA retained 75% initial activity after ten cycles. Kinetic parameters V_max and K_m of the free and immobilized PGAs were determined as 0.113 mmol/min/mg-protein and 0.059 mmol/min/mg-protein, and 0.68 mM and 1.19 mM, respectively. Synthesis of amoxicillin with the immobilized PGA was carried out in 40% ethylene glycol at 25 ℃ and a conversion of 72% was obtained. These results showed that the immobilization of PGA onto magnetic chitosan nanoparticles is an efficient and simple way for preparation of stable PGA.