• Title/Summary/Keyword: pH study

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A Comparison Study on Functional Properties of Peanut Protein and Chemically Modified Peanut Protein (분리 땅콩 단백질과 화학적으로 수식화한 단백질간의 식품학적 특성 비교)

  • Sohn, Kyung-Hee;Min, Sung-Hee;Park, Hyun-Kyung;Park, Jin
    • Korean journal of food and cookery science
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    • v.7 no.2
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    • pp.97-104
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    • 1991
  • This study was carried out in order to study the protein functionality such as foaming and emulsifying properties by succinylation of peanut protein isolates. Succinylated and unsuccinylated peanut protein isolate was tested for to find out the effect of pH, heat treatment and sodium chloride concentration on the solubility, foam expansion, foam stability, emulsion capacity and emulsion stability. The results are summarized as follows; 1. Succinylation enhanced the solubility of peanut protein isotate (PPI). The solubility of succinylated PPI markedly increased at pH 4.5. When the protein solutions was heated, the solubility of succinylated PPI greatly increased than PPI at pH 3. With addition of NaCl, solubility of succinylated PPI increased at pH 7 and pH 9. 2. The foam expansion of PPI and succinylated PPI on pH was no difference between both proteins. Addition of NaCl and heat treatment caused steeply increased in foam expansion at pH 3. 3. The foam stability of PPI and succinylated PPI showed the lowest value at pH 4.5. When PPI and succinylated PPI was heated, foam stability of two proteins incensed at pH 3 and showed similar aspects between PPI and succinylated PPI. However, at pH 9 stability of succinylated PPI decreased by heat treatment over $60^{\circ}C$. 4. Emulsion capacity of succinylated PPI on pH was markedly increased and showed the highest value at pH 11. At pH 4.5 which is isoelectric point of PPI, emulsion capacity of PPI by succinylation improved than that of PPI. When succinylated PPI was heated, emulsion capacity was greatly increased at pH 2 and pH 7. With NaCl was added, emulsion capacity of succinylated PPI increased than that of PPI. 5. Emulsion stability of PPI and succinylated PPI was affected by pH and showed its highest value at pH 11. At pH 4.5, emulsion stability of succinylated PPI increased than that of PPI. Addition of NaCl and heat treatment caused slightly increased in emulsion stability of succinylated PPI.

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Regulation of Intracellular pH by SHC1 in Saccharomyces cerevisiae (효모에서 SHC1 유전자의 이온 농도 조절에 의한 세포내 pH 항상성 유지)

  • 하승길;전준철;최의열
    • Korean Journal of Microbiology
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    • v.38 no.3
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    • pp.168-172
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    • 2002
  • Budding yeasts maintain an effective system to regulate intracellular pH in response to environmental pH fluctuation. In a previous study we reported that SHC1 plays a role in cell growth at alkaline condition, not at acid pH. We constructed a null mutant deleted an entire open reading frame for SHC1. To test whether the retardation in cell growth was caused by the absence of intracellular pH buffering capacity, we measured intracellular pH with a pH-sensitive fluorescent dye, C.SNARE. The intracellular pH of the mutant cell was much higher than that of wild-type cells, indicating that the mutant cells lack some types of buffering capacity. We also investigated the level of $Na^+ and K^+$ content with atomic mass spectroscopy after alkali shock. Wild-type cell showed a higher level of intracellular K^+$ content, whereas there was no difference in $Na^+$ level. The result suggested that K^+$ is more important in the regulation of intracellular pH in yeasts.

A Study of Sex Difference by Chemical Determination of Dentin in Human Teeth (치아경조직의 화학적 판정에 의한 성차 연구)

  • 장세활;김종열
    • Journal of Oral Medicine and Pain
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    • v.8 no.1
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    • pp.127-142
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    • 1983
  • This thesis was to investigate sex defferencd by employing the methodes of Bernadskij's and it's modification by means of the evaluation of pH of dentin in 162 extracted teeth. The obtained were as follows: 1. In 72% of all experinments, pH of human dentin substance was higher in female than in male. Mean pH was 13.175±0.031 in male and 13.245±0.030 in female. 2. After comparative studies on each pH in anatomical region of tooth arrangement, the sex difference of pH was most obviously showed in molars. 3. In pH change according to ages, the 30's was the highest in male and the 20's in female, also pH showed decresing tendency by the increasing of age in both male and female. 4. There was no pH difference in anatomical regions of tooth arrangment. 5. Mean pH was 13.282±0.013 in under 50 years of age and 13.282±0.022 over 50. It proved that pH was higher in under 50 years of age than in over 50.

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Micro pH Sensor Using Patterned Hydrogel with pH Indicators

  • Jang, Ji-Sung;Kwon, Sung-Hoon
    • Journal of Sensor Science and Technology
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    • v.20 no.4
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    • pp.234-237
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    • 2011
  • This paper presents a study into pH Indicator-Embedded hydrogel micro-particles which are encoded various shapes according to the captured indicator. We incorporate various pH indicators into a photo-curable hydrogel, PEG-DA(Poly(ethylene glycol) diacrylate). Using the latest fluidic lithography techniques, we can easily fabricate variously patterned hydrogel particles based on in-situ photopolymerization of the PEG-DA in a micro-fluidic channel. The shape of the particle is related to the pH indicator inside the particle. We demonstrate that the micro pH sensors change their colors according to the pH levels. The micro pH sensors have various characteristics that are related to the curing time, particle size, etc. By changing these conditions, we can adjust the long term stability and reaction time of the hybrid micro pH sensors.

The Study on Bleaching of Kenaf Fibers (Part II) -Effect of Strength and Elongation- (케냐프 섬유의 표백에 대한 연구 (제2보) -강도와 신도의 변화를 중심으로-)

  • Lee, Hye-Ja;Yoo, Hye-Ja;Han, Young-Sook
    • Journal of the Korean Society of Clothing and Textiles
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    • v.29 no.11
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    • pp.1454-1464
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    • 2005
  • The study was based on a three-stage, non-repetitive factorial experiment in which chemical-rotted kenaf fibers were treated separately with hydrogen peroxide concentrations of $0.5\%,\;1\%\;and\;2\%$, with pH solutions of 7, 9 and 11, and treatment times of 30, 60 and 90 minutes. Under optimal conditions, the study was conducted to determine the strength and elongation of kenaf fibers by the addition of chelators, penetrants and surfactants. The hydrogen peroxide concentration, solution pH and treatment time directly affected the strength of kenaf fibers. The hydrogen peroxide concentration, solution pH affected the elongation of kenaf fibers. It was found, however, that the interaction between pH and treatment time, concentration and treatment time, concentration and treatment time and pH affected the strength of kenaf fibers. Also, It was found that the interaction between pH and concentration, concentration and treatment time, concentration and treatment time and pH affected the elongation of kenaf fibers. Under the hydrogen peroxide conditions of $2\%$ concentration, pH 11 and a treatment time of 60 minutes, there were no effects on the strength and elongation of kenaf fibers with the addition of chelator SP, CA.

The Effect of pH on the Formation of Furfural Compounds in the Glucose and Fructose with Amino Acid Enantiomers in Maillard Reaction

  • Kim, Ji-Sang;Lee, Young-Soon
    • Preventive Nutrition and Food Science
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    • v.13 no.1
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    • pp.54-59
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    • 2008
  • This study was conducted to investigate the effect of pH on the formation of furfural compounds from glucose and fructose reacting with amino acid enantiomers in the Maillard reaction. Hydroxymethylfurfural (HMF) content was highest at pH 4.0, and decreased with increasing pH. HMF was significantly higher in glucose-based systems than fructose-based systems. Furfuryl alcohol (FFA) and 5-methyl-2-furaldehyde (MF) were not increased with increasing pH, and only small amounts were formed. In addition, 2-furaldehyde (F) was found to increase in the systems, as pH increased. However, the content was small and variable. 2,5-Dimethyl-4-hydroxy-3(2H)-furanone (DMHF) was only found in Glc/D-Asn, Glc/L-Lys and Fru/D-Lys system, but the content was not increased with increasing pH. 2-acetylfuran (AF) was higher in Glc (or Fru)/L-Lys and Glc (or Fru)/D-Lys systems at pH 7.0. However, at pH 4.0, the content of AF was higher in the Glc (or Fru)/Gly and Glc (or Fru)/L-Asn systems. Therefore, this study aimed to observe the effect of pH, sugars and amino acid enantiomers on the production of furfural and related compounds by the Maillard reaction. A clear tendency was observed for some classes of compounds to be more easily formed at higher or lower pH. HMF was more readily formed at lower pH, while FFA, F, DMHF and MF were inhibited by acidic conditions. Particularly, compounds like FFA, F and MF were not affected by pH changes. In addition, DMHF and MF were only formed in L-Lys and D-Lys system.

THE REMINERALIZING EFFECTS OF EARLY ENAMEL CAR10US LESION BY SUPERSATURATED BUFFER SOLUTION UNDER PH CYCLING MODEL (pH 순환 모델에서 과포화 용액의 초기 우식 법랑질에 대한 재광화 효과)

  • 김소라;홍석진;노병덕;이찬영;금기연
    • Restorative Dentistry and Endodontics
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    • v.26 no.4
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    • pp.341-349
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    • 2001
  • Dental caries is the most common oral disease. There are many factors contributing to its development, but complete understanding and prevention are not fully known. However, it is possible to remineralize the early enamel curious lesion by fluoride containing remineralization solution. Recently the pH-cycling model has been used to examine the effect of fluoride solution on remineralization of artificial caries in vitro as it can closely simulate the conditions encountered in vivo within a carefully controlled environment. The aim of this study was to evaluate the remineralizing effects of supersaturated buffer solutions under pH-cycling model. The specimen with 3mm-diameter was made using mature bovine incisors which has no caries and has sound enamel surface. Early curious lesions were produced by suspending each specimens into demineralization solution at pH 5.0 for 33 hours and the specimen whose surface hardness value ranged from 25 to 45 VHN were used. The pH cycling treatment regimen consisted of 5 min soaks of three treatment solutions four times per days for 15 days and the continuous cycling of demineralization and remineralization were carried out for 15 days. Following the pH-cycling treatment regimen, the specimens' surface microhardness were measured by the Vickers hardness test (VHN) and analyzed by ANOVA and Duncan's multiple-range test. 1. The surface microhardness value of supersaturated solution, Senstime, and Gagline groups were increased after pH cycling, and that of supersaturated solution was significantly Increased compared to saline group(P<0.05). 2. The surface remineralization effect of fluoride containing solutions was accelerated by saliva under pH-cycling mode 3. The pH cycling model was considered appropriate to mimic the intra-oral pH changes when evaluating demineralization and remineralization in vitro. Under the results of above study, salivary remineralization effect can be improved by fluoride containing remineralization solution. The pH-cycling model was considered appropriate to mimic the intra-oral pH changes when evaluating demineralization and remineralization in vitro.

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Experimental Study on pH Reduction by Neutralization Treatment and Curing Methods of Porous Concrete for Planting (식생을 위한 다공성 콘크리트의 중성화 처리 및 양생방법에 따른 pH 저감에 관한 실험적 연구)

  • 성찬용;김영익
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.44 no.2
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    • pp.99-106
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    • 2002
  • This study is performed to examine pH reduction by neutralization treatment and curing condition of porous concrete using rice straw ash for planting. Test results show that pH of porous concrete without neutralization treatment in the dry and water curing is 10.34 ∼ 10.57 and 9.42 ∼ 9.72, respectively. pH of porous concrete by neutralization treatment in the dry and water curing is 9.72 ∼ 10.03 and 9.00 ∼ 9.37, respectively. Accordingly, the best method for pH reduction of porous concrete for planting is to use water curing and neutralization treatment.

A Study on Artificiality Salivary pH and Sugar Fermentation Test of Caries Potentiality Foods (우식유발식품에 의한 인공타액내 pH 변화와 당 분해 효소 활성에 관한 연구)

  • Ji, Yun-Jeong;Park, Su-Kyung
    • Journal of dental hygiene science
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    • v.12 no.4
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    • pp.303-309
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    • 2012
  • This study was designed to provide basic data for developing a standardized caries potentiality index to help to choose snack foods with a low score on the index by investigating adolescents' snack intakes and measuring them in total saccharinity, pH, and acidogenic potential and glucosidase activity of Streptococcus mutans (S. mutans). Total 28 snack foods were selected and measured for total saccharinity (Pocket refractometer PAL-1, ATAGO) and pH with a pH meter (Mentor, Seoul, Korea). Artificial saliva was added to each sample. The experiment group was administered with S. mutans (ATCC 3692), cultured in a constant temperature incubator at $37^{\circ}C$, and measured for pH changes over five times including after 10 minutes, 30 minutes, 1 hour, and 24 hours. Each sample of 0.3 ml was added to tubes containing sucrose, galactose, or glucose to measure the glucosidase activity of S. mutans. They were then observed for glucosidase activity with colorimetry after 24 hours of culture in a constant temperature incubator at $37^{\circ}C$. The mean pH by the acidogenic potential of S. mutans was pH 5.33. The experiment group dropped in pH more than the control group due to the increasing acidogenic potential of S. mutans by glucosidase activity, recording pH 5.27 after 10 minutes, pH 5.21 after 30 minutes, pH 5.15 after 1 hour, and pH 4.80 after 24 hours. The observation results of glucosidase activity of S. mutans with colorimetry show that most of the samples were positive in orange and yellow with glucose, sucrose, and glactose recording activity of 78.58%, 75%, and 71.42%, respectively.

A study on the dyeing-rate in single and mixture dyeing of silk with levelling and milling acid dyes (견섬유에 대한 산성 Levelling 및 Milling 염료의 단일 및 혼합염색에 있어서의 염색속도에 관한 연구)

  • 배도규
    • Journal of Sericultural and Entomological Science
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    • v.33 no.1
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    • pp.9-12
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    • 1991
  • A study on dyeing-rate for single and mixture dyeing of silk with acid dyes was carried out. Single and mixture dyeings were performed with levelling and milling dyes at 7$0^{\circ}C$ and at pH 3, pH 4 and pH 5, respectively. The results of this study were summerized as follows : 1. C. I. Acid Orange 7. A) At pH 3, dyeing-rate was more fast in the single dyeing than in mixture dyeing at the beginning of dyeing process. B) At pH 4 and 5, dyeing-rates tend to get very similar in single and mixture dyeing. 2. C. I. Acid Blue 138 A) At all pH value, dyeing-rates were more fast in the mixture dyeing than single dyeing at the beginning of dyeing. B) As the time increased, the difference of dyeing-rate and adsorption amounts between single and mixture dyeing was not shown.

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