• Journal of the korean academy of Pediatric Dentistry
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• 제43권2호
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• pp.137-144
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• 2016

The aim of this study was to evaluate the effect of a range of acidic pH values on the push-out bond strength and surface morphology of tricalcium silicate materials: Biodentine$^{(R)}$, Theracal$^{(R)}$ and ProRoot MTA$^{(R)}$. The standardized lumens of root slices prepared from extracted single-root human teeth were filled with Biodentine$^{(R)}$, Theracal$^{(R)}$ and ProRoot MTA$^{(R)}$ according to manufacturer's instructions. The specimens were randomly divided into 4 groups (n = 20) for each material and then incubated for 4 days at $37^{\circ}C$; 3 acidic groups (butyric acid buffered at pH 4.4, 5.4, 6.4) and 1 control group (phosphate buffered saline solution at pH 7.4). The push-out bond strengths were then measured by using a universal testing machine and the surface morphology of each experimental group was analyzed by a scanning electron microscope. Biodentine$^{(R)}$ and Theracal$^{(R)}$ showed higher push-out bond strength compared with ProRoot MTA$^{(R)}$ after exposure to acidic pH values. A substantial change in the surface morphology of each material occurred after exposure to different pH values. In conclusion, the push-out bond strengths of Biodentine$^{(R)}$ and Theracal$^{(R)}$ are higher than the ProRoot MTA$^{(R)}$. Further the acidic environment weakens the push-out bond strength and microstructure of tricalcium silicate materials.

• Journal of the Korean Society of Food Science and Nutrition
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• 제26권1호
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• pp.67-71
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• 1997

Different concentrations of lignin and hemicellulose were preincubated with 0.01$\mu\textrm{g}$ of 2-amino-3-methylimidazo［4, 5-f］quinoline(IQ) at simulated gastrointestinal pH condition s. The Ames Salmonella assay using Salmonella typhimurim TA98 was performed to detect any changes in the mutagenicity of IQ in the presence of lignin or hemicellulose. IQ revealed very weak or no mutagenicity when it was preincubated at pH 2.1. However, there was a dose-dependent inhibition of IQ mutagenicity in tile presence of lignin or hemicellulose at pH 5.4 and 6.6. The antimutagenic activities of fibers were not different from each other. Also, at lower concentrations of fibers, pH 5.4 was more effective in suppressing IQ mutagenicity, while 300$\mu\textrm{g}$ of lignin or hemicellulose significantly reduced the mutagenicity of IQ regardless of pH conditions. These results suggest that at gastrointestinal pH conditions, both soluble and insoluble fibers inhibit mutagenicity of IQ by adsorption. Therefore, a possible mechanism for Protective effect of fibers against cancer is due to their adsorption to mutagens in the gastrointestinal tract, and pH seems to be an important factor in the regulation of interactions between the fiber and mutagens.

• KSCE Journal of Civil and Environmental Engineering Research
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• 제37권1호
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• pp.73-82
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• 2017

In Korea, previous certification of water supply infrastructure was mainly focused on economical and physical aspects. Recently, hygienic safety of water supply service has become a sensitive and important issue to our people for evaluating the water quality with growth of economy and education system. According on water quality in 497 Korean water supply facilities, pH values in the supplied water have ranged between 5.8-8.5. However, little is known about metal leachability at the pH conditions observed in the real water supply systems because a fixed pH condition (pH 7.0) has been used in the current standard method, 'Hygienic Safety Testing Method', in water supply. In this work, we examined the effects on heavy metal leachability with pH differences in the water supply pipes which are typically used in Korea. As a result, the amounts of metal leachability were tended to increase when pH levels were decreased. Especially at pH 5.8, Cu leachability from Cu pipes was found to exceed the public health standard level even after applying a normalization factor (NF) given by the current Korea standard method. The Cr and Cu leached from stainless steel pipes, Cd, Pb, Cu, and Zn from Cu-based pipe fittings, and Zn from Zn-based pipe fittings were exceeded the Korean hygienic safety standards while, after applying the NF, concentrations of the leached metals were satisfied with the current Korean standard. The findings from this work provide implications on the needs of reforming the current hygienic safety standard methodology.

• Journal of the Korea Organic Resources Recycling Association
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• 제26권1호
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• pp.29-37
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• 2018

In this study, optimum extraction conditions for phosphorus recovery from sewage sludge ash(SSA) were investigated. For this purpose, an experiment was conducted to determine optimal recovery conditions for Ca-P type phosphorus by using calcium component in the recycled aggregate residue. The phosphorus content of sewage sludge ash was confirmed to be 5.0 %. When $H_2SO_4$ was used as an extract, concentration of 1 N $H_2SO_4$, L/S ratio of 10, and extraction time of 30 min were found to be the optimal extraction conditions. Phosphorus was extracted by using optimal extraction conditions, and then the heavy metals eluted with phosphorus were removed using 1~20 g of cation exchange resin. In 20 g of cation exchange resin, Fe 71.3%, Cu 82.4%, Zn 79.9%, and Cr 15% were removed. After that, the mixing ratio of the calcium extract obtained from the recycled aggregate residue (RAR) was changed to 1:1, 1:5, 1:10. The pH of the SSA to RAR mixture was adjusted to 2, 4, 8 and 12 by the addition of 5 N NaOH to the mixture of 1:5, and the phosphorus was recovered as Ca-P type precipitate. The optimum pH was 8. When recycled aggregate residues were used, the weight of calcium phosphate increased, but the amount of wastewater generated also increased. Therefore, it was concluded that the use of recycled aggregate residue was not economically feasible.

• The Korean Journal of Food And Nutrition
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• 제23권1호
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• pp.63-69
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• 2010

With the increase of the use of antibiotics and invasive procedures, infections caused by multidrug-resistant Acinetobacter baumannii(MRAB) are increasing. We screened the antibiotic producing strain B-51 for antibacterial activity against MRAB from the soils and studied the effects of culture medium on the antibiotic production of B-51. The medium conditions for maximum antibiotic productivity of B-51 was 2% glycerol, 0.5% soybean meal, 0.01% $CaCl_2$, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.01% $KH_2PO_4$ at an initial pH of 6.0, at $30^{\circ}C$ for 76 h.

• Journal of Life Science
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• 제9권2호
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• pp.169-175
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• 1999

Salmonella typhimurium can encounter a wide variety of environments during its life cycle. In nature, S. typhimurium can experience and survive dramatic acid stresses that occur in diverse ecological niches ranging from pond water to phagolysosomes. These survival mechanism is aquired by the Acid Tolerance Response(ATR) in Salmonella. The ATR of S. typhimurium is a complex inducible phenomenon in which exposures to slight or moderate low pH will produce a stress response capable of protecting the organism against more severe acid challenges. ATR in Salmonella has two different systems that are called RpoS dependent and independent. We found that ATR in anaerobic was showed RpoS independent because rpoS$\Omega$AP had ATR as S. typhimurium UK1. Using the P22 MudJ(Km, lacZ) operon fusion technique and a lethal selection procedure combining low pH(pH4.5) and sodium acetate(10mM, pH4.5), we isolated LF487 aatA::MudJ which showed acid sensitive in anaerobic condition. aatA locus was determined at 12 min on Salmonella Genetic Map. The survival rate of aatA mutant was showed significantly diminished at pH4.3 than virulent wild type Salmonella in anaerobic condition(5% $CO_2$, 5% H$_2$, 90% $N_2$). Therefore isolated gene was confirmed important gene for anaerobic ATR system.

• Korean Journal of Soil Science and Fertilizer
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• 제29권3호
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• pp.243-248
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• 1996

To find out the oxidation process of potential acid sulfate soil(PASS) along with time. the PASS were treated with lime and ammonia water to adjust soil pH in laboratory column condition. pH range of PASS showed 6.5 to 7.5. however, complete oxidized PASS by $H_2O_2$ showed 2.1 to 2.5. After pilling the PASS under the natural condition. oxidation occured slowly from surface of the pilled soil. The oxidation of PASS proceeded slowly when the soil was in submerged condition. but quickly in dried condition. The content of sulfide-sulfur in PASS sharply decreased after exposing to the air and the decreasing rate was greater in dried than in submerged condition. The content of sulfate-sulfur continuously decreased in submerged condition. but increased in dried condition. Contents of $Fe^{+{+}}$ and $Al^{+{+}}$ in PASS were generally increased with time and the increasing rate was greater in submerged than in dried condition. Liming to PASS was slowly acting to pH change and ammonia water caused fast pH change within a short period of time. The contents of sulfate-sulfur and exchangeable aluminum in drainage water decreased with time and the contents of sulfide-sulfur and ferrous iron were increased.

• Radiation Oncology Journal
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• 제17권1호
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• pp.70-77
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• 1999

Purpose : The expression of p53, P211WAF/CIP, Bcl-2, and Bax underlying the radiation-induced apoptosis in different pH environments using SCK mammary adenocarcinoma cell line was investigated. Materials and Methods Mammary adenocarcinoma cells of hi) mice (SCK cells) in exponential growth phase were irradiated with a linear accelerator at room temperature. The cells were irradiated with 12 Gy and one hour later, the media was replaced with fresh media at a different pHs. After Incubation at 37Microbioiogy, College of Medicine Dong A University for 0$\~$48 h, the extort of apoptosis was determined using agarose gel electrophoresis and flow cytometry. The progression of cells through the cell cycle after irradiation in different pHs was also determined with flow cytometry. Western blot analysis was used to monitor p53, p211WAFfCIP, Bcl-2, and Bu protein levels. Results : The induction of apoptosis by irradiation in pH 6.6 medium was markedly less than that in pH 7.5 medium. The radiation-induced G2IM arrest in pH 6.6 medium lasted markedly longer than that in pH 7.5 medium. Considerable amounts of p53 and p21 proteins already existed at pH 7.5 and increased the level of p53 and p21 significantly after 12 Gy X-irradiation. An incubation at pH 6.6 after 12 Gy X-irradiation did not change the level of p53 and p21 protein levels significantly. Bcl-2 proteins were not significantly affected by radiation and showed no correlation with cell susceptibility to radiation-induced apoptosis in different pHs. An exposure to 12 Gy of X-rays increased the level of Bax protein at pH 7.5 but at pH 6.6, it was slight. Conclusions : The molecular mechanism underlying radiation-induced apoptosis in dinerent pH environments using SCK mammary adenocarcinoma cell line was dependent of the expression p53 and P211YVAF/CIP proteins. We may propose following hypothesis that an acidic stress augments the radiation-induced G2iM arrest, which inhibiting the irradiated cells undergo post-mitotic apoptosis. The effects of environmental acidity on anti-apoptotic and pro-apoptotic function of Bcl-2 family was unclear in SCK mammary adenocarcinoma cell line.

• Korean Journal of Food Science and Technology
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• 제26권1호
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• pp.18-22
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• 1994

This study was designed to investigate the method of the simultaneous determination of water-soluble vitamins (thiamine, ascorbic acid, riboflavin, folic acid, cyanocobalamine, pyridoxin, nicotinic acid) by free soloution capillary electrophoresis. Factors affecting on the separation were pH of the buffer, applied voltage and temperature. On-column detection at 254 nm allowed accurate and reproducible determination of these compounds. All these compounds were separated within ca. 8 min with fused silica capillary at 20 kV.

• Journal of the Korean Society of Food Science and Nutrition
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• 제20권2호
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• pp.179-186
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• 1991

The nutritional requirement and cultural condition such as carbon and nitrogen sources, cultural temperature, initial pH, cultural time and aeration for the production of endocellular cytosine deaminase from Aspergillus fumigatus IFO 5840 were investigated. The cultural broth giving maximum cytosine deaminase yield was found to consist of 2% glucose as a carbon source and 1% yeast extract and 0.1% peptone as a nitrogen source. Optimal initial pH of the culture broth was pH 8.5 and the enzyme production in the cell usually reached a maximum after 28 hours of cultivation in the 500ml shaking flask containing 100ml broth at $30^{\circ}C$. The endoenzyme production of the used strain was inhibited by inorganic nitrogen, but activated by orgainc nitrogen, yeast extract.