The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Screening and Optimal Culture Conditions of Antibiotic-Producing Actinomycetes B-51 for Multidrug Resistant Acinetobacter baumannii

다제내성 Acinetobacter baumannii에 유효한 방선균 B-51의 탐색 및 이 균주가 생산하는 항생물질 발효 최적 배양 조건

  • Received : 2010.02.18
  • Accepted : 2010.03.05
  • Published : 2010.03.30
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Abstract

With the increase of the use of antibiotics and invasive procedures, infections caused by multidrug-resistant Acinetobacter baumannii(MRAB) are increasing. We screened the antibiotic producing strain B-51 for antibacterial activity against MRAB from the soils and studied the effects of culture medium on the antibiotic production of B-51. The medium conditions for maximum antibiotic productivity of B-51 was 2% glycerol, 0.5% soybean meal, 0.01% $CaCl_2$, 0.01% $MgSO_4{\cdot}7H_2O$ and 0.01% $KH_2PO_4$ at an initial pH of 6.0, at $30^{\circ}C$ for 76 h.

다제내성균 A. baumannii에 유용한 항생물질을 생산하는 방선균을 선별하기 위하여 토양에서 분리한 방선균 300여 균주를 대상으로 항균 활성을 실험하여 그 활성이 가장 뛰어난 B-51 균주를 최종적으로 선별하였다. 이 균으로부터 항생물질 생산을 위한 최적 조건을 조사하기 위하여 발효 배지를 기본배지로 하여 동일조건 하에서 탄소원, 질소원, 무기염 및 인삼염만을 다르게 하여 항균 활성을 실험한 결과 glycerol 2%, soybean meal 0.5%, $CaCl_2$ 0.01%, $MgSO_4{\cdot}7H_2O$ 0.01%, $KH_2PO_4$ 0.01%에서 항생물질 생산이 가장 높았다. 또한 이렇게 결정된 배지 조성에서 초기 pH 6.0, 배양온도 $30^{\circ}C$, 배양시간 76시간 조건 하에서 항생물질 생산이 가장 많았다. 항생물질을 생산하는 대부분의 방선균이 항생물질 생산 시 배양액의 final pH가 중성 혹은 약 알칼리인 점을 비교해 볼 때 본 균주인 B-51의 경우 항생물질 생산능이 높은 경우 final pH 4.0 부근인 산성 pH를 보여 그간의 논문보고와는 상반되는 결과를 보였다.

Keywords

References

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