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Comparison of Sperm Morphology Evaluation Using Strict Criteria, Acrosome Reaction Following Ionophore Challenge and Zona-free Hamster Ova Sperm Penetration Assay as Prognostic Factors in Diagnosis of Male Infertility and In Vitro Fertilization (남성 불임의 진단 및 체외수정의 예후인자로서 정자 형태의 정밀 분석과 정자 첨체반응 및 햄스터 난자 침투 분석의 비교 연구)

  • Moon, Shin-Yong;Ryu, Buom-Yong;Pang, Myung-Geol;Oh, Sun-Kyung;Lee, Jae-Hoon;Suh, Chang-Suk;Kim, Seok-Hyun;Choi, Young-Min;Kim, Jung-Gu;Lee, Jin-Yong
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.1
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    • pp.57-66
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    • 2002
  • Objective : This study was designed to investigate the interrelationship and clinical usefulness of sperm morphology by strict criteria (SM), acrosome reaction following ionophore challenge test (ARIC) and sperm penetration assay (SPA) using zona-free hamster ova as prognostic factors in in vitro fertilization. Materials and Methods: Semen samples were provided by 83 patients undergoing IVF. We first evaluated the differences between normal fertilization group and poor fertilization group on three andrologic tests. Secondly, we analyzed the relationship between the three andrologic tests and in vitro fertilization on IVF settings. Finally, we evaluated the effectiveness of the three andrologic tests as the prognostic indicators for fertilizing ability. Results: The fertilization rate of all men in the poor fertilization group was less than 30%; but there was no evidence that this poor fertilization was due to oocyte defects. The results of three andrologic tests were significatly higher in normal fertilization group. Fertilization rate (%) in vitro was highly correlated (p<0.001) with % normal sperm by SM, ARIC value (%), and SPA result. By using Receiver-Operator-Characteristic curve (ROC), we evaluated the effectiveness of these three tests. The sensitivity and specificity of SM, ARIC test and SPA in predicting fertilization potential in IVF setting were 76% and 75%, 84% and 90%, and 76% and 95%, respectively. Conclusion: Our data suggest that the three andrologic tests can be reliable tools as prognostic factors of sperm fertilizing ability. Among these test, ARIC test and SPA gave more accurate information on fertilizing capacity. ARIC test was shown to have a predictive value for fertilizing ability comparable to that of SPA that appears to be a simple and cost-effective addition to current andrology laboratory. Combined application of these three tests may give more information on predicting sperm fertilizing capacity.

Studies on Weed Control with Herbicides in Soybean Field (콩밭 잡초방제에 관한 연구)

  • Ryang Whan Seung
    • Korean journal of applied entomology
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    • v.10 no.1
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    • pp.31-38
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    • 1971
  • Tolerance test in plastic vat, pot and fold tests were carried out to investigate the selective herbicides for soybean culture in sandy loam. The soybean plants showed great tolerance against herbicides such as Tri-allate (Avadex-BW), Alachlor (Lasso), Butachlor (Machete), Propachlor (Ramrod), Nitrofen (TOK), MO, HE-314, Nitrofen/Dinoseb(TOK/DNBP), and Chlo.oxu.on (Tenoran), and the growth was normal even when each was treated with the herbicides up to 2ft3 times of the recommended concentrations. Soybean plants showed a slight tolerance against Prometryne (Gesagard), Propazine (Gegamil), Diuron (Karmex), Metabromuron (Patoran), Linuron (Lorox) and Swep when each was treated with herbicides up to 1-2 times of the recommended concentrations. Great injury or withering was noticed due to the high sensitivity of soybean to Simazine (CAT) and to Floumetron (Cotoran). In pot and field experiments with herbicides such as Butachlor (Machete), Alachlor (Lasso), Nitrofen (TOK), Kerb, Nitrofen/Dinoseb (TOK/DNBP), Swep, Linuron (Lorox), Simazine (CAT) and PCP, the following results were obtained: Great injuries were noticed with Simazine (CAT). Also, Linuron (Lorox) and Kerb showed a slight injury at early growth stage of soybean, Nitrofen (TOK) , Nitrofen/Dinoseb (TOK/DNEP), Alachlo. (Lasso), Butachlo. (Machete) and Swep had high selectivities for soybean and no injury was noticed. With respect to herbicidal effects there was a greatly significant difference between treated plots and non-treated plots with the exception of Simaaine (CAT) plot in field test. E. crusgalli and C. sanguinalis were tolerant against Simazine(CAT) and Linuron(Lorox). Cyperus and E. annuus were tolerant against Kerb. Great herbicidal effects on grasses were observed in Alachlor (Lasso) and Butachlor (Machete) plots. Among broad-leaf weeds, P. hydropiper and C. album were tolerant against Butachlor (Machete) and Alachlor (Lasso). When soybean was treated with the herbicides such as Alachlor (Lasso) (ai. 150g/10a), Butachlor (Machete) (ai. 300g/10a), Nitrofen (TOK) (ai. 250g/10a), Linuron (Lorox) (ai. 75g/10a) once after seeding, no additional wording was required till harvest.

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Effect of light touch on body sway during a stable posture with blocked visual information

  • Kim, Jong-Gun;Kim, Jin-Hong;Do, Kwang-Sun;Yim, Jongeun
    • Physical Therapy Rehabilitation Science
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    • v.5 no.3
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    • pp.138-142
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    • 2016
  • Objective: The purpose of this study was to investigate how light touch with a finger affects balance ability when a posture is maintained in the condition of visual information blockage and to provide a fundamental material for developing balance ability in the process of rehabilitation treatment. Design: Cross-sectional study. Methods: The study subjects were 17 healthy men and women in their twenties and thirties who were studying at S University in Seoul. The system was comprised of an equilateral triangular force platform. Subjects were asked to step on the foot position of the force platform (Good Balance, Finland) barefooted for 30 seconds, with eyes closed, hands hanging down loosely, and feet comfortably apart. It was connected to a laptop by using Bluetooth technology. An experiment was conducted in the following three circumstances: 1) no-touch trial, 2) light touch to the back (T7 area), and 3) light touch to the middle finger of the left hand. Each subject was given a 10-minute break between consecutive measurements. The experimental circumstances were performed randomly. Anteroposterior sway (APSV), mediolateral sway velocity (MLSV), and velocity moment (VM) were measured. Results: The APSVs (mm/s) were $9.32{\pm}3.37$ and $5.45{\pm}2.98$; the MLSVs (mm/s), $6.39{\pm}3.35$ and $3.31{\pm}2.48$; and VM ($mm^2/s$), $17.13{\pm}11.75$ and $6.76{\pm}8.31$ in the first and second experimental circumstances, respectively. APSV, MLSV, and VM values were significantly improved with the 1) no-touch trial and 2) light touch to the back trail conditions compared with the 3) light touch to the middle finger of the left hand condition (p<0.05). Conclusions: This study revealed that the balance ability for maintaining a body posture was influenced more by light touch to the back (T7) than by light touch with the sensitive fingertip and body sway diminished after visual information was blocked.

Quantification of Thermal Insulation by Clothing Items and Analysis of Influencing Factors (단일의복의 보온력 정량화와 영향 요인)

  • Baek, Yoon Jeong;Hwang, Soo Kyung;Lee, Hyo Hyun;Park, Joonhee;Kim, Do-Hee;Lee, Joo-Young
    • Journal of the Korean Society of Clothing and Textiles
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    • v.42 no.1
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    • pp.172-182
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    • 2018
  • The purpose of the present study was to quantify the thermal insulation of garments by item and examine factors influencing clothing insulation. A total of 769 garments in clo unit were collected and classified into 12 categories: blouses/shirts (95 items, BS), T-shirts/sweaters (62 items, TS), vest (23 items, VT), cardigans (23 items, CD), jackets/coats (75 items, JC), sport outerwear (including padding jackets)(48 items, SO), trousers (23 items, TR), skirts (56 items, SK), dresses (28 items, DS), underwear (150 items, UW), sleepwear (50 items, SW), and personal protective clothing (59 items, PPC). The results showed that clothing insulation was $0.21{\pm}0.01clo$ for the BS, $0.22{\pm}0.01clo$ for TS, $0.12{\pm}0.00clo$ for VT, $0.23{\pm}0.02clo$ for CD, $0.40{\pm}0.02clo$ for JC, $0.49{\pm}0.03clo$ for SO, $0.21{\pm}0.01clo$ for TR, $0.18{\pm}0.01clo$ for SK, $0.34{\pm}0.03clo$ for DS, $0.09{\pm}0.01clo$ for UW, $0.42{\pm}0.03clo$ for SW, and $0.56{\pm}0.03clo$ for PPC (p<.001). The most influential factors among the seven factors for thermal insulation of garments were clothing weight and covering area; however, the explanatory powers of two factors differed according to clothing categories. The covering area had more significant impact on clothing insulation in cardigans, jackets/coats, trousers, and dresses than clothing weight. Covering areas and clothing weight were the most influential factors in the following categories: blouses/shirt, T-shirts/sweaters, skirts, sleepwear and personal protective clothing. The garment weight was the most important factor for thermal insulation for the sport outerwear.

A study on the state of customized visiting oral health programs (맞춤형 방문구강보건사업 현황조사)

  • Jung, Jae-Yeon
    • Journal of Korean society of Dental Hygiene
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    • v.9 no.4
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    • pp.606-619
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    • 2009
  • Objectives : This study was to examine the state of customized visiting oral health programs in a bid to help facilitate the unified operation of the programs and the development of required guidelines. Methods : The subjects in this study were 49 dental hygienists who were professionals responsible for customized visiting health care programs across the nation. Results : 1. Regarding the form of employment of the dental hygienists were investigated many contract and daily workers. 2. As to the possession of equipment necessary for visiting oral health programs, denture cleaners(12.2%) were most widely possessed in some regions, followed by mobile scalers(10.2%) and mobile suctions(8.2%). In terms of expendable devices and materials, dental mirrors, pincettes and explorers were the most widely possessed dental checkup devices, and the most widely possessed oral hygiene supplies were toothbrushes, interdental brushes and denture cleaners. Those devices and materials were in more possession than the other types of devices and materials. The most widely possessed equipment for educational purpose was laptop computers, followed by beam projectors and screens. The most widely possessed teaching materials were dentiform, followed by CD-ROMs. 3. Those whom they visited the most for oral health care service were elderly people, followed by the disabled and patients with chronic diseases. The dental hygienists who went out to visit those people outnumbered the others who stayed at public health centers. Concerning the types of visiting oral health care service, the most prevalent service provided to the elderly included denture cleaning/management, oral massage and preventive treatment against dental caries. The most dominant service provided to the disabled involved education of the oral health care act, preventive treatment against dental caries and toothbrushing by professionals. The most common service offered to patients with chronic diseases was education of the oral health care act and oral health education. The dental hygienists paid a visit to a mean of 5.8 households a day. The average weekly number of households cared by the dental hygienists was 27.3. It took a mean of 37.1 minutes for them to take care of each household. 4. As for satisfaction level with the implementation of the visiting oral health programs, they expressed the greatest satisfaction at teamwork with professionals($3.56{\pm}0.94$), followed by the professionalism of their work($3.21{\pm}0.94$) and workload($3.08{\pm}0.94$). Their satisfaction level with the work conditions required for creative job performance($2.75{\pm}0.98$) and partnership with other institutions($2.64{\pm}1.03$) was below 3.0. In regard to the impact of their characteristics, marital status made a statistically significant difference to satisfaction level with workload. The unmarried dental hygienists were more pleased with their workload than the married ones(p<0.05). 5. As to needs for education for professionalism improvement, they asked for education about visiting oral health care skills the most, followed by education about oral health care for patients with chronic diseases, education of planning/evaluation and education of oral health care for the disabled. Conclusions : The top priority for the vitalization of the programs was the procurement of budget, followed by the procurement of equipment and educational media and the procurement of human resources.

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Effects of Continual Pre-plant Application of Pig Slurry on Soil Mineral Nutrients and Yield of Chinese Cabbage (액비의 밑거름 연용이 배추의 수량 및 토양 양분 함량에 미치는 영향)

  • Lim, Tae-Jun;Lee, In-Bog;Kang, Seok-Beom;Park, Jin-Myeon;Hong, Soon-Dal
    • Korean Journal of Environmental Agriculture
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    • v.28 no.3
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    • pp.227-232
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    • 2009
  • Land application of animal manure, such as pig slurry(PS), has been considered as valuable organic source to supply necessary nutrients for crop growth. On the other hand, little information is available on the effect of consecutive application of PS on agricultural soils. This study was conducted to evaluate the influence on change in soil chemical properties and yield of Chinese cabbage in soils amended with slurry composting and bio-filtration pig slurry (SCB) for four consecutive crop cultivations (2007-2008). Among total application rates required, a basal fertilizer (35%) was applied with SCB or chemical fertilizer (CF). The CF was applied as a side dressing was applied in all treatments. There was no significant effect of SCB and CF on Chinese cabbage yield during four cropping seasons. In addition, soluble sugar, vitamin C contents and yields of Chinese cabbage between SCB and CF did not show statistically significant difference. During the experiment, soil chemical properties between SCB and CF treatment showed similar pattern, however, the contents of exchangeable sodium (Na) in soil has been increased (P<0.05) since the second cropping season because of higher Na contents in SCB. Therefore, the use of SCB as a substitute of CF is available for growth and yield of chinese cabbage while its long-term application might leads to an accumulation of Na in soil.

The Effects of Adipose Derived Stem Cells on Neurogenic Differentiation and Induction of Nerve Regeneration (인체 지방조직에서 유래한 줄기세포의 신경세포 분화능 및 신경재생 유도효과)

  • Jun, Young Joon;Rhie, Jong Won;Choi, Yun Seok;Kim, Young Jin;Kim, Sung Eun;Lee, Jong In;Han, Ki Taik
    • Archives of Plastic Surgery
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    • v.33 no.2
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    • pp.205-212
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    • 2006
  • Using adipose derived stem cells(ASCs), neurogenic differentiation was induced in a mono layered culture medium containing neuronal induction agents. Cells differentiated to the neuronal cells were observed with a inverted microscope and immunofluorecent study. We made a 15 mm long defect in the sciatic nerve of 14 rats and connected a silicone tube to the defect. Then, we mixed neuronal progenitor cells differentiated from ASCs with collagen gel and grafted them to a group of rats(experimental group) and grafted only collagen gel into another group(control group). In 4 and 8 weeks after the graft, histological observation was made. According to the result, the number and diameter of myelinated axons were significantly increased in the experimental group. In addition, the nerve conduction velocity was improved more in the experimental group and neovascularity also increased. Moreover, reaction with S100 and p75 was observed in regenerated nerves in the experimental group, suggesting that the grafted cells were differentiated into supportive cells such as Schwann's cells. In conclusion, this research proved that ASCs can multiply and differentiate into neuronal cells. If they are grafted into nerve defects, the grafted cells are differ entiated into supportive cells such as Schwann's cells and thus contribute to nerve regeneration. Accordingly, the use of adipose tissue obtained easily without the limitation of donor site can be greatly helpful in treating peripheral nerve defects.

Effects of Neurofeedback Training and Computer-Assisted Cognitive Rehabilitation on Cognition and Upper Extremity Function in PostStroke. (신경되먹임 뇌파 훈련과 컴퓨터보조 인지 재활훈련이 뇌졸중 환자의 인지와 상지기능에 미치는 영향)

  • Jung, Min-Woo;Shim, Sun-Hwa
    • Therapeutic Science for Rehabilitation
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    • v.1 no.1
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    • pp.57-70
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    • 2012
  • Objective : This study was to evaluate the effects of a Neurofeedback(NFB) and Computer Assisted Cognitive Rehabilitation(CACR) training to improve on cognition and affected arm function in stroke subjects. Methods : Participants were randomly allocated to three groups: NFB(n=14), CACR(n=14) and control(n=16). All groups received occupational therapy and physical therapy for 5 session 30 minutes per week during 6 weeks. Also NFB and CACR group practiced additional NeuroComp training and RehaCom training for 30 sessions 30 minutes during 6 weeks. Results were evaluated by cognition, affected arm function. Results : There were significantly increased by CACR training that outcomes of MMSE-K(p<.05). And there weren't significantly difference by NFB and CACR training that outcomes of the affected arm function. And a difference between three groups wasn't found. Conclusion : The NFB and CACR training improves cognitive function. These results suggest that NFB and CACR training is feasible and suitable for individuals with stroke.

Comparison of Three Methods Assessing the Ergonomic Risks of Manual Lifting Tasks at Ship Engine Manufacturing Facilities (선박용 엔진 제조업 들기작업의 인간공학적 위험 평가를 위한 세 가지 방법 비교)

  • Kim, Sun Ja;Shin, Yong Chul;Kim, Boo Wook;Kim, Hyun Dong;Woo, Ji Hoon;Kang, Dongmug;Lee, Hyun Seok
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.15 no.2
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    • pp.104-113
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    • 2005
  • A variety of ergonomic assessment methods of lifting tasks known as a major cause of work-related lower back pain have been used. But there is a limited information in choosing the most appropriate assessment method for a particular job and in finding out strengths and weakness of the methods. The purpose of this study was to assess and compare the ergonomic risks of lifting tasks in a marine diesel engine production industry by three lifting ergonomic assessment tools widely used: the National Institute for Occupational Safety and Health(NIOSH) Revised Lifting Equation(NLE), the Washington Administrative Code 296-62-0517(WAC), and the Snook Tables. Lifting index(weight of load/Recommended Weight Limit) of NLE($LI_{NLE}$) was above 1 at 34 tasks(75.6%) of a total number of 45 lifting tasks. LI of WAC($LI_{WAC}$) was above 1 at 11 tasks(24.4 %). LI of Snook Table($LI_{Snook}$) was above 1 at 29 tasks(64.4%). Thus, LI was high in orders of $LI_{NLE}$ > $LI_{Snook}$ > $LI_{WAC}$. There were significantly high correlations among three Lls(p<0.01). The correlation coefficients between $LI_{NLE}$and the other three Lls($LI_{WAC}$ and $LI_{Snook}$) were r=0.93 and r=0.88, respectively. The linear regression equations were y = 0.444x + 0.11(r=0.93) between $LI_{NLE}$ and $LI_{WAC}$, y = 0.93x + 0.008(r=0.88) between LI(NLE) and $LI_{Snook}$. The LI values by WAC was significantly lower than those by the other tools. The compared features, strength and limitation among these tools were described in this paper.

Use of Complementary and Alternative Medicine among Cancer Patients in Korea (우리나라 소화기암 환자들의 대체의료이용에 관한 연구)

  • Jung, Eun-Young;Han, Dong-Woon;Choi, Byung-Hee;Kim, You-Kyum;Park, Yeon-Hee
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.6
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    • pp.1590-1596
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    • 2007
  • Complementary and alternative medicine (CAM) has gained in popularity among cancer patients in recent years. The use of CAM in cancer patients is common with about one third of patients using some form of CAM in Western countries. The purpose of this study was to analyze the use of CAM and determine what factors affect to use CAM among cancer patients to provide CAM therapy information and assist therapy selection among various CAM therapies to cancer patients. The design of the study was descriptive cross-sectional, and data were collected using a 16-item questionnaire. This study was conducted in subjects with confirmed diagnosis of stomach, colon, liver, or pancreas cancer, in both out-patients clinics and inpatients setting in a tertiary hospital in Seoul Korea. As a result, among the participants, past or current CAM use was reported by 75%, which shows a statistically significant difference in income groups(P<0.05), but no difference in age and religion groups. The most common therapies use by cancer patients included traditional Korean medicine (32.1%), folk remedies (26.6%), exercise (14%), dietary supplements (11.6%), physical therapy (9.9%), diet therapy (5%), and meditation (4%). 77.8% of patients show satisfaction and 64.4% shows perceived effectiveness of CAM. Male patients with higher income, and previous treatment were more likely to use CAM. The main benefits from CAM reported by cancer patients were psychological improvement and symptom improvement. Of the cancer patients used CAM, 30.9% were dissatisfied, 25.8% did not have benefits from the use, and 7.6% experience side effects. Cancer patients who prefer CAM (more than 3 kinds) used it to cure cancer, on the contrary, the one who do not prefer CAM used to improve symptoms and psychological stability. The main sources of information about CAM were family and friends(54.4%), and media(24.5%), doctor and nurse(18.3%), and religion group(2.6%). Findings suggest that due to the relatively high use of CAM among cancer patients in Korea, this topic should be taken into account in the development of a holistic approach to cancer patients and efficient cancer patients management system and proactive and consistent management of CAM is necessary in the health care system in Korea.