• 제목/요약/키워드: p23

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강원도(江原道) 춘천시(春川市) 지역(地域)에 있어서 5개(個) 수종(樹種) 수간류(樹幹流)의 pH 및 전기전도도(電氣傳導度) 변화(變化) (Variations of pH Values and EC of Stemflow of 5 Tree Species in Chunchon Districts, Kangwon-do)

  • 김민식;서문원;전근우
    • 한국산림과학회지
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    • 제90권4호
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    • pp.413-419
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    • 2001
  • 강원대학교 산림과학대학 부속연습림의 대표적 조림수종인 잣나무와 낙엽송, 천연생 활엽수인 신갈나무, 굴참나무와 침엽수인 소나무 등 5종류, 각 2본씩 합계 10본의 강우와 수간류의 pH값 및 EC값을 분석한 결과는 다음과 같다. 1. 수간류의 월평균 pH값은 강우의 pH값보다 낮게 나타났으며, 침엽수인 소나무, 잣나무 및 낙엽송 보다 활엽수인 굴참나무와 신갈나무의 pH값이 높게 나타났다. 수종별 pH값은 잣나무 3.23~5.03(평균 : 4.23), 소나무 3.57~5.16(평균 : 4.36), 낙엽송 3.41~6.69(평균 : 4.45), 굴참나무 3.86~6.11(평균 : 4.70), 신갈나무 4.39~6.13(평균 : 5.18)였으며, 강우의 pH값과는 직접적인 상관이 없는 것으로 나타났다. 2. 수간류의 월평균 EC값은 침엽수종이 활엽수종보다 약 1.5~2배이상 높게 나타나, 잣나무 $29.0{\sim}946.5{\mu}S/cm$(평균 : $258.5{\mu}S/cm$) > 소나무 $18.6{\sim}907.5{\mu}S/cm$(평균 : $220.3{\mu}S/cm$) > 낙엽송 $27.0{\sim}918.0{\mu}S/cm$(평균 : $216.2{\mu}S/cm$) > 굴참나무 $16.5{\sim}301.6{\mu}S/cm$(평균 : $125.4{\mu}S/cm$) > 신갈나무 $25.2{\sim}225.7{\mu}S/cm$(평균 : $101.5{\mu}S/cm$)순으로 나타났다. 3. 수간류의 평균 pH값과 EC값은 계절적인 차이가 나타났다. 즉 pH값은 봄에는 낮고, 여름과 가을에 높게 나타났으며, EC값은 봄에는 높고, 여름과 가음에는 낮게 나타났다. 4. 강우량에 따른 pH 변화는 뚜렷하게 나타나지는 않았으나, 침엽수와 활엽수 모두 적은 강우에서 낮은 pH값을 나타냈다. 그러나 EC값은 모든 수종에서 50mm 이하의 강우에서 $100{\mu}S/cm$ 이상으로 높게 나타나 강우량에 따른 차이가 확인되었다.

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다당류를 이용한 수용성 난황 단백질의 분리 (Separation of water-soluble egg yolk proteins using polysaccharide)

  • 문태화;이현종
    • Applied Biological Chemistry
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    • 제36권4호
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    • pp.296-303
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    • 1993
  • 식품첨가물로 허용되어 있는 알긴산나트륨, 알긴산프로필렌글리콜(PGA), 카르복시메틸셀룰로오스나트륨(CMC), 펙틴 등 4종의 다당류를 이용하여 달걀 노른자의 수용성 단백질을 분리하기 위한 조건을 반응표면 분석법으로 조사하였다. Rotatable hexagon design으로 실험을 계획하여 다당류의 사용농도 5개 수준, 반응 pH 3개 수준에서 상층액 중의 단백질과 지방질 함량을 각각 측정하여 2차 회귀모형을 구하였다. 통계분석 결과 반응 pH가 다당류의 농도보다 난황 단백질-다당류 상호작용에 더 영향을 미치는 요인으로 밝혀졌다. 각 반응도의 contour plot을 작성, 분석하여 수용성 난황 단백질 분리의 조건으로 알긴산나트륨; 농도 $0.23{\sim}0.25%$, $pH\;5.9{\sim}6.0$, PGA; 농도 $0.15{\sim}0.17%$, $pH\;4.3{\sim}4.5$, CMC; 농도$0.30{\sim}0.31%$, pH 3.0 펙틴; 농도 $0.09{\sim}0.10%$, $pH\;5.6{\sim}5.8$을 설정하였다.

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Aspergillus sp. (C-58)균주가 생산하는 Inulin 분해효소에 관한 연구 - Aspergillus sp. C-58균주가 생산하는 inulase P-I의 정제 - (Studies on the Inulin Hydrolyzing Enzyme from Aspergillu sp. (C-58) (III) - Purification of inulase (P-I) from Aspergillus sp. (C-58) -)

  • Kwon, Tae-Jong;Seu, Jung-Hwu
    • 한국미생물·생명공학회지
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    • 제11권1호
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    • pp.47-52
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    • 1983
  • Aspergillus sp.C-58 균주가 생산하는 extracellular inulase에 대하여 pH, charcoal처리 및 ammonium sulfate로 분별염석한 후 DEAE- cellulose를 이용한 column chromatography에 의하여 3개의 효소단백질(Peak I, II, III)로 분획되었으며 그 비율은 31. 1 : 1.7 : 1이였다. P- I, II의 I/S는 그 비율이 0.23 및 0.24로 거의 동일하였으나 P-III는 1.1로 P-I및 P-II와 상이하였다. Peak I 효소에 대하여 DEAE-Sephadex A-50을 이용한 ion exchange chromatography에 의하여 추출효소에 비교하여 약 408배 정제되었으며 다시 Sephadex G-75 및 Sephadex G-100에 2회 gel filtration하여 약 482배 정제되었다. 이상과 같이 정제한 Peak I의 효소액은 poly acrylamide를 이용한 disc gel electrophoresis 및 ultra centrifugation에 의하여 단일 단백질로 확인되었다.

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Effect of Anti-Aging Standard Forest Healing Program With Multiple Visits to a Forest Facility on Cognition in Older Age Patients

  • Jinseok Park;Sheng-Min Wang;Dong Woo Kang;Beom Lee;Hojin Choi
    • 대한치매학회지
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    • 제23권1호
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    • pp.44-53
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    • 2024
  • Background and purpose: The anti-aging standard forest healing program (ASFHP), which uses forest therapy, was reported to be effective in improving psychological, physical, and cognitive functions. However, there are several challenges to directly visiting the forest. This study aimed to investigate the impact of multi-session ASFHP with forest visit on the mental and physical health of the older people with visits to forest facilities and compared them with those of the same program conducted indoors. Methods: Individuals aged over 70 years with concerns about cognitive decline were recruited at dementia relief centers and divided into control and experimental groups. A total of 33 people were administered ASFHP under the supervision of a forest therapy instructor. The control group stayed indoors, while the experimental group visited a forest healing center and repeated the program 20 weeks. Results: The multiple-session ASFHP positively affected cognitive impairment screening test (CIST) total scores (p=0.002), memory (p=0.014), Korean version of the Repeatable Battery for the Assessment of Neuropsychological Status total scores (p<0.001), immediate recall (p=0.001), visuospatial/construction (p<0.001), language (p<0.001), forest healing standard questionnaire total scores (p=0.002), and cognitive function (p=0.019), regardless of location. The forest visits during the ASFHP showed positive effects on orientation (p=0.035), delayed recall (p=0.042), emotional stability (p=0.032), physical activity (p=0.005), and health (p=0.022). The CIST scores of the memory domain were the strongest indicator of the multiple-session ASFHP effects. Conclusions: The 20-week multi-session ASFHP with forest visit showed effects on cognitive improvement and physical and emotional stability compared to indoor education.

고등식물 형질전환용 유전자 운반체 pKCHI의 개발 (Development of a Plant Transformation Vector, pKCHI)

  • 정상호
    • Journal of Plant Biology
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    • 제32권1호
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    • pp.23-32
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    • 1989
  • We have developed a plasmid vector, pKCH1, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning sites, Bam HI and Xba I, between them. After inserting a foreing gene at the cloning sites, P35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind III.

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Association of Genetic Polymorphisms at 1q22 but not 10q23 with Gastric Cancer in a Southern Chinese Population

  • Yang, Xue-Xi;Li, Fen-Xia;Zhou, Cui-Ping;Hu, Ni-Ya;Wu, Ying-Shong;Li, Ming
    • Asian Pacific Journal of Cancer Prevention
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    • 제13권6호
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    • pp.2519-2522
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    • 2012
  • Objective: Data from a recent genome-wide association studiesy of gastric cancer (GC) and oesophageal squamous cell carcinoma in Chinese living in the Taihang Mountains of north-central China suggest that 1q22 and 10q23 are susceptibility-associated regions for GC. However, this has not been confirmed in southern Chinese populations. The aim of this study was to investigate whether these polymorphisms at 1q22 and 10q23 are associated with the risk of GC in a southern Chinese population. Methods: We selected seven top significant associated single nucleotide polymorphisms (SNPs) at 1q22 and 10q23 and conducted a population-based case-control study in a southern Chinese population. Genotypes were determined using MassARRAYTM system (Sequenome, San Diego, CA). Results: Two SNPs at 1q22, rs4072037 and rs4460629, were significantly associated with a reduced risk of GC, best fitting the dominant genetic model. Logistic regression models adjusted for age and sex showed that rs4072037 AG and GG (OR=0.64, P=0.017, compared with AA) and rs4460629 CT and TT (OR=0.54, P=0.0016, compared with TT) significantly reduced the risk of GC. However, no significant results for the five SNPs at 10q23 were obtained in this study. Conclusion: These outcomes indicate that 1q22 is associated with GC susceptibility in this southern Chinese population, while an association for the locus at 10q23 was not confirmed.