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Performance, Meat Quality and Blood Composition of Cross Bred Chicks Fed Various Organic Dietary CP and ME (ME와 CP가 다른 유기사료 급여가 토종닭의 생산능력 계육품질 및 혈액성상에 미치는 영향)

  • Park, Jae-Hong;Jeong, Yong-Dae;Yoon, Myung-Ja;Ryu, Kyeong-Seon
    • Korean Journal of Organic Agriculture
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    • v.19 no.2
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    • pp.199-214
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    • 2011
  • This study was conducted to investigate the effect of organic dietary metabolizable energy (ME) and crude protein (CP) on productivity, meat quality and blood composition of crossbred chicks (hanhyub 3 ho) for different growring periods. Experiments were factorially designed with ME 3,000, 3,100kcal/kg and CP 21, 22, 23% for starter (0-4wks); ME 3,100, 3,150, 3,200kcal/kg and CP 18, 19, 20% for grower (5-8wks); ME 3,100, 3,150, 3,200kcal/kg and CP 15, 16, 17% for finisher (9-10wks). The total number of chicks and replicate of each treatment for starter, grower, finisher were 720, 4; 702, 6; 468, 4, respectively. The diets was mixed with more 90% organic feed ingredients. The productivity were not influence by dietary ME for starting period. Weight gain and feed intake were significantly increased in CP 23% treatment than CP 21, 22% treatment (P<0.05). FCR was improved as dietary CP increased (P<0.05). Weight gain was tended to be increased by decreaing ME content and increasing CP contents for growing period. Feed intake showed no difference among the ME and CP treatment groups. FCR improved significantly in CP 19, 20% compared with CP 18% (P<0.05). Weight gain was not significantly different between ME and CP treatments for finishing period. Feed intake was lower in ME 3,150, 3,200kcal/kg than the ME 3,100 kcal/kg treatment (P<0.05). FCR was higher in ME 3,100kcal/kg than ME 3,150, 3,200kcal/kg treatment (P<0.05). Interaction on productivity was not exited between ME and CP treatment groups for different feeding periods. There was no difference in the total protein, albumin, glucose and triglyceride amount in blood depending on dietary ME and CP contents. Total cholesterol was greatly decreased in ME 3,100kcal/kg than the ME 3,200kcal/kg (P<0.05), but not different between CP treatments. Cooking loss of breast meat was lower in ME 3,150kcal/kg than the ME 3,200kcal/kg (P<0.05). Moisture, shear force and pH were not statistically different among treatments. Protein solubility was increased by increasing ME and CP in diets. The collagen was tended to increase as dietary ME increased. Redness was remarkably higher in ME 3,150kcal/kg than the ME 3,100kcal/kg (P<0.05). Yellowness of meat fed ME 3,150kcal/kg showed significantly lower than other treatments (P<0.05). Therefore, the optium ME and CP to improve the productivity for each period were 3,000kcal/kg, 23%; 3,100kcal/kg, 19%; 3,150kcal/kg, CP 16%, repectively.

Purification and characterization of bromelain isolated from pineapple (파인애플 bromelain의 정제 및 특성)

  • Suh, Hyung-Joo;Lee, Ho;Cho, Hong-Yon;Yang, Han-Chul
    • Applied Biological Chemistry
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    • v.35 no.4
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    • pp.300-307
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    • 1992
  • Bromelains (EC 3.4.4.24) isolated from pineapple fruit and stem have been purified about 18 and 46-folds to homogenity in the same yield of 23%. Molecular weights of fruit and stem-bromelain were estimated to be 32.5 KDa and 37 KDa by Sephadex G-200, respectively. The enzymes were composed of one subunit. The fruit and stem-bromelain had their maximum activity at pH 8.0, $70^{\circ}C$ and at pH 7.0, $60^{\circ}C$. Especially the enzymes catalyzed hydrolysis of plant proteins such as ISP (Isolated soybean protein) and wheat gluten with high molecular activity compared to animal proteins. The enzymes were competitively inhibited by sulfhydryl reagent; $K_i$ values of fruit and stem-bromelain for pCMB(p-chloromercuribenzoate) were 0.18 mM and 0.10 mM. Activities of the enzymes inhibited by pCMB were reversibly restored with increasing concentration of cysteine.

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The Effect of Sexual Knowledge, Sexual Behavior and Self-efficacy on their Reproductive Health Promoting Behaviors in Female College Students (여대생의 성지식, 성행동, 자기효능감이 생식건강증진행위에 미치는 영향)

  • Kim, Bo-gyung;Sung, Mi-Hae
    • Journal of East-West Nursing Research
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    • v.23 no.1
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    • pp.18-25
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    • 2017
  • Purpose: The purpose of this study was to identify factors which effect to female college student' reproductive health promoting behavior in female college students. Methods: The design of this study was correlational study and subjects were 127 female college students from two colleges in B metropolitan city. The data collection was carried out from June 1, 2016 to June 30, 2016. Data were collected using questionaires of characteristics of the subjects, sexual knowledge, sexual behavior, and self-efficacy(KGSE). Results: The subjects' reproductive health promoting behavior differed according to the grade, major, smoking, and experience of weight loss of more than 10 kg within the last 3 months. The subjects' reproductive health promoting behavior had positive correlations with sexual knowledge (r=.39, p<.001) and self-efficacy (r=.34, p<.001), and negative correlation with sexual behavior (r=-.23, p=.011). Sexual knowledge and sexual behavior were influencing factors on reproductive health promoting behavior accounting for 23.0%. Conclusion: This study showed that sexual knowledge and sexual behavior were factors affecting female college students' reproductive health promoting behavior and the most influential factor was sexual knowledge.

MiR-323-5p acts as a Tumor Suppressor by Targeting the Insulin-like Growth Factor 1 Receptor in Human Glioma Cells

  • Lian, Hai-Wei;Zhou, Yun;Jian, Zhi-Hong;Liu, Ren-Zhong
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.23
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    • pp.10181-10185
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    • 2015
  • Background: MicroRNAs, small noncoding RNA molecules, can regulate mammalian cell growth, apoptosis and differentiation by controlling the expression of target genes. The aim of this study was to investigate the function of miR-323-5p in the glioma cell line, U251. Materials and Methods: After over-expression of miR-323-5p using miR-323-5p mimics, cell growth, apoptosis and migration were tested by MTT, flow cytometry and cell wound healing assay, respectively. We also assessed the influence of miR-323-5p on the mRNA expression of IGF-1R by quantitative real-time reverse transcriptase PCR (qRT-PCR), and on the protein levels by Western blot analysi. In addition, dual-luciferase reporter assays were performed to determine the target site of miR-323-5p to IGF-1R 3'UTR. Results: Our findings showed that over-expression of miR-323-5p could promote apoptosis of U251 and inhibit the proliferation and migration of the glioma cells. Conclusions: This study demonstrated that increased expression of miR-323-5p might be related to glioma progression, which indicates a potential role of miR-323-5p for clinical therapy.

In Vivo Evaluation of the Safety of Hydrolyzed GMP Powder containing Highly Concentrated Sialic Acid (23%) produced by Enzyme Separation and Solvent Enrichment Method using Micronucleus Test in Mice (효소분리 및 용매정제법으로 제조한 고농도 Sialic Acid(23%)가 함유된 GMP 가수분해분말의 마우스 골수세포의 소핵시험을 이용한 안전성 평가연구)

  • Kim, Hee-Kyong;Cho, Hyang-Hyun;Noh, Hye-Ji
    • Journal of Dairy Science and Biotechnology
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    • v.34 no.2
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    • pp.83-89
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    • 2016
  • This study was designed to determine the mutagenic potential of hydrolyzed glycomacropeptide (GMP) powder (hereafter referred to as 23%-GNANA; product name: HELICOBACTROL-23) in a micronucleus test using bone marrow in ICR mice. Three experimental groups were used: a 3-step concentration group, with a maximum concentration of 2,000 mg/kg, and other sequentially two-fold lower concentrations, a negative control group, and a positive control group. The test material was administered for 2 d to observe the frequency of micronucleus formation up to 48 h after the test material was absorbed by the body. When the polychromatic erythrocyte (PCE) content of erythrocytes was compared, no significant differences were noted between the negative control group and the test group (p<0.05). Similarly, when the average numbers of micronucleated PCE (MNPCE) in 2,000 PCE per animal were compared, no significant difference was observed between the negative control group and the test group (p<0.05). No dose-response relationship with regard to the concentration of the test material administered was noted. These results allow us to conclude that hydrolyzed whey protein powder does not cause formation of micronuclei in mouse bone marrow cells under the applied conditions. In this study, the average frequency of micronucleus formation in PCE was significantly higher in the positive control group compared with the negative control group; thus, the test conditions were appropriate for detecting the frequency of micronucleus formation induced by the test material. In conclusion, the safety of 23%-GNANA test substance was verified in an in vivo micronucleus test in mice, conducted before the registration of HELICOBACTROL-23 as a food additive.

Study on Rate-Limiting Factors with a Heavy Loaded Biofilter

  • Son, Hyun-Keun
    • Journal of Korean Society for Atmospheric Environment
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    • v.23 no.E2
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    • pp.74-81
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    • 2007
  • Feasibility test for a biofilter was performed to treat VOCs. The applied loading rate to the biofilter was calculated between 60 to $3,700\;kg\;COD/m^3$. Trimethyl-pentene and trimethyl-hexene were the two most dominant compounds and they occupy about 85 percent. During the acclimation period, it is desirable for a biofilter to receive relatively lower VOCs concentration and flow rate, until it can adjust to new substrate and operational environment. Temperature at various points inside the biofilter reactor was observed with more than 23 temperature sensors. With steam heating, temperatures of the top sections of the media were greater than those of bottom sections. Without steam heating, intermediate stages generally had higher temperature measurement than those of bottom and top stages. Because the pH values for different biofilter materials vary significantly, measurement of the pH for the mixture of different combinations of biofilter materials is necessary. Based on the types and brands of media, the measured pH ranged from 5.38 to 9.10. The range of measured pH of different mixtures with perlite, compost, saw dust, peat moss, limestone, vermiculite was 7.05 to 8.62.

Has Eco-friendly Management Influence on the Customer's Purchasing Intention at Franchise Korean-restaurant

  • Yoon, Tae-Hwan
    • Culinary science and hospitality research
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    • v.23 no.6
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    • pp.11-19
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    • 2017
  • The purpose of this article was to study the influence of eco-friendly management on customer's purchasing-intention at franchise Korean restaurants. In this research, factor analysis and multi regression analysis were used. Eco-friendly management was divided into 4 factors. Eco friendly service affected positively brand image (p<0.05). Energy saving positively affected brand image (p<0.005). Contribution to local society positively affected brand image (p<0.05). Menu composition affected the most positively brand image (p<0.001). At last, brand image had positive influence on purchasing intention (p<0.001). According to these results, we confirmed that the factors of eco friendly management influenced significantly on the customers' perception of brand image. As a result, food service corporations need to deal with the factors of eco friendly management efficiently. The findings of this research would help business management to build effective service marketing strategies and to satisfy the needs of customers at franchise Korean restaurants.

The Electrochemical Properties of Supercapacitor for Smart Grid Energy Storage System with Variation of MWCNT/Super P Content (스마트 그리드 에너지 저장시스템 슈퍼커패시터의 MWCNT/Super P 함량에 따른 전기 화학적 특성)

  • Lee, Du-Hee;Lee, Kyoung-Min;Yoon, Jung-Rag
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.23 no.11
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    • pp.843-847
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    • 2010
  • This paper in concerned with the electrochemical properties for supercapacitor of composition with variation of super P/MWCNTs(multi-walled carbon nanotubes) contents. Electrochemical properties of the super P/MWCNTs were measured by cyclic voltammetry at the scan rates of 0.5 mV/s is $TEABF_4$(tetra-ethyl-ammonium-tetra-fluoro-borate) as electrolytes. As a result, the composition for 6 wt% content of MWCNTs led to an increase of capacitance, but DC resistance were decreased. It was found that the content and dispersion appearance of MWCNTs was attributed to the increase in capacitance and lower DC resistance.

Relations among Suicidal Ideation, Parental Attachment, and Self-Identity of College Students (대학생의 자살생각, 부모에 대한 애착과 자아정체감과의 관계)

  • Kim, Hyo-Jeong
    • Journal of East-West Nursing Research
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    • v.17 no.2
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    • pp.132-138
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    • 2011
  • Purpose: The purpose of this study was to explore the relationship among suicidal ideation, parental attachment, and self-identity of college students. Methods: This study was designed as a descriptive correlational research. The participants were 314 college students in Gyeongbuk province. Data were collected from September 23 to Oct 5 in 2009 using a structured questionnaire. Data were analyzed by t-test, ANOVA, and Pearson correlation coefficient using SPSS WIN (Version 12.0). Results: Suicidal ideation showed negative correlations with maternal and paternal attachment (r=-.20, p<.001; r=-.24, p<.001, respectively) as well as self-identity (r=-.37, p<.001). Self-identity had positive correlations with maternal and attachment (r=.16, p=.006; r=.23, p<.001, respectively). Conclusion: The findings suggest that suicidal ideation may be decreased when college students have higher parental attachment and self-identity. Strategies to improve parental attachment and self-identity are needed in near future.