• Asian-Australasian Journal of Animal Sciences
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• v.18 no.5
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• pp.601-605
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• 2005

Three lines of White Leghorn (WL) chickens (IWJ, IWG and IWC) maintained at Central Avian Research Institute, Izatnagar (UP), were used for chorioallantoic membrane (CAM) and liver tumour (LT) assay. Eleven-day-old embryos of each line were partitioned into three groups and inoculated with 0.2 ml of subgroup A, subgroup C and an equal mixture of subgroup A and C Rous sarcoma virus (RSV). Subgroup virus receptor on the cell surface membrane for subgroup A is coded for by tumour virus a (tva) locus and for subgroup C by tumour virus c (tvc) locus. The random association of the genes at the tva and tvc loci in IWJ and IWC line was assessed and the $x^2$-values for phenotypic classes were found to be significant, indicating the linkage between the tva and tvc loci. The linkage value was estimated to be 0.09 on pooled sex and pooled line basis. On the basis of four subclass tumour phenotypes a 4-allele model was proposed for tva locus having $a^{s1}$, $a^{s2}$, $a^{r1}$ and $a^{r2}$ alleles and the frequencies were calculated as 0.47, 0.13, 0.13 and 0.27 for IWJ line, 0.31, 0.33, 0.14 and 0.22 for IWG line and 0.44, 0.11, 0.21 and 0.24 for IWC line, respectively. Similarly, for tvc locus the frequencies of four alleles i.e. $c^{s1}$, $c^{s2}$, $c^{r1}$ and $c^{r2}$ were calculated as 0.42, 0.20, 0.21 and 0.17 for IWJ line, 0.42, 0.17, 0.27 and 0.14 for IWG line and 0.30, 0.21, 0.16 and 0.33 for IWC line, respectively. The $x^2$-values for all classes of observations were not significant (p>0.05), indicating a good fit to the 4-allele model for the occurrence of 4-subclass tumour phenotypes for tva and tvc loci. On the basis of the 2-allele model both tva and tvc locus carries three genotypes each. But, on the basis of the 4-allele model tva and tvc locus carries 10 genotypes each. The interaction between A-resistance and C-resistance (both CAM and LT death) was ascertained by taking the 10 genotypes of tva locus and 3 genotypes of tvc locus by pooling the lines and partitioning the observations into 3 classes. The $x^2$-values for the genotypic classes of CAM (-) LT (+) and CAM (-) LT (-) phenotypes to mixed virus (A+C) infection were found to be highly significant (p<0.01), indicating increased resistance, which indicates the joint segregation of $a^r$ and $c^r$ genes, suggesting the existence of close linkage between the tva and tvc loci. Therefore, an indirect selection approach using subgroup C viruses can be employed to generate stocks resistant to subgroup A LLV, obviating contamination with the most common agent causing LL in field condition.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.1032-1037
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• 2009

The present experiment was conducted to study the effects of partial replacement of spray-dried porcine protein (SDPP) or fish meal with soybean and shrimp protein hydrolysate (SSPH) on growth performance, nutrient digestibility and serum biochemical parameters in weaned pigs. Two hundred and forty 21${\pm}$2 d old pigs ((Pietrain${\times}$Duroc)${\times}$(Landrace${\times}$Large Yorkshire)) with initial weight of 6.9${\pm}$0.5 kg were randomly allocated to five dietary treatments with six replicates per treatment and eight piglets per replicate. The control diet (T1) contained 2% SDPP and 6% fishmeal, and SDPP for experimental diets T2 and T3 was replaced with 1% and 2% SSPH, respectively, on an iso-nitrogenous basis. The fishmeal for experimental diets T4 and T5 was replaced with 1% and 2% SSPH, respectively, also on an iso-nitrogenous basis. The experimental period was 21 days. The results showed that weaned piglets fed the diets containing 1% and 2% SSPH as a replacement for SDPP had similar average daily gain (ADG), average daily feed intake (ADFI), feed/gain (F/G), diarrhea rate and serum biochemical indices e.g. blood urea nitrogen (BUN), total serum protein (TP), albumin to globulin ratio (A/G), globulin (GLO), serum glucose (GLU), and immunoglobulin G (IgG) to those fed the control diet during 0-10 d and 0-21 d of the experiment. The substitution of 1% SSPH on an iso-nitrogenous basis for fish meal appeared to be beneficial for ADG (p = 0.59) and ADFI (p = 0.23) of piglets during the overall period. The digestibility of calcium was higher (p<0.01) in pigs fed diets containing SSPH than on the control diet. Addition of 1% SSPH on an iso-nitrogenous basis for fish meal could increase the digestibilities of dry matter and energy of the diet. Dietary replacement of fish meal with 1% and 2% SSPH had no effect on the concentrations of BUN, TP, A/G, GLO, GLU, and IgG. In conclusion, dietary SDPP or fish meal could partially replace SSPH without any adverse effect on growth performance, nutrient digestibility and serum biochemical parameters in weaned piglets.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1583-1591
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• 2015

The present study was conducted to determine the effect of lipid sources with different fatty acid profiles on nutrient digestion and ruminal fermentation. Ten rumen and duodenal fistulated Nellore steers (268 body weight${\pm}27kg$) were distributed in a duplicated $5{\times}5$ Latin square. Dietary treatments were as follows: without fat (WF), palm oil (PO), linseed oil (LO), protected fat (PF; Lactoplus), and whole soybeans (WS). The roughage feed was corn silage (600 g/kg on a dry matter [DM] basis) plus concentrate (400 g/kg on a DM basis). The higher intake of DM and organic matter (OM) (p<0.001) was found in animals on the diet with PF and WF (around 4.38 and 4.20 kg/d, respectively). Treatments with PO and LO decreased by around 10% the total digestibility of DM and OM (p<0.05). The addition of LO decreased by around 22.3% the neutral detergent fiber digestibility (p = 0.047) compared with other diets. The higher microbial protein synthesis was found in animals on the diet with LO and WS (33 g N/kg OM apparently digested in the rumen; p = 0.040). The highest C18:0 and linolenic acid intakes occurred in animals fed LO (p<0.001), and the highest intake of oleic (p = 0.002) and C16 acids (p = 0.022) occurred with the diets with LO and PF. Diet with PF decreased biohydrogenation extent (p = 0.05) of C18:1 n9,c, C18:2 n6,c, and total unsaturated fatty acids (UFA; around 20%, 7%, and 13%, respectively). The diet with PF and WF increased the concentration of $NH_3-N$ (p<0.001); however, the diet did not change volatile fatty acids (p>0.05), such as the molar percentage of acetate, propionate, butyrate and the acetate:propionate ratio. Treatments PO, LO and with WS decreased by around 50% the concentration of protozoa (p<0.001). Diets with some type of protection (PF and WS) decreased the effects of lipid on ruminal fermentation and presented similar outflow of benefit UFA as LO.

• Journal of Life Science
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• v.10 no.2
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• pp.210-217
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• 2000

A kinetic profile of the catalytic domain of stromelysin-1 (SCD) using the fluorescent peptide substrate has been determined by the stopped-flow technique. The pH profile has a pH optimum of about 5.5 with an extended shoulder above pH 7. Three pKa values, 5.0, 5.7, and 9.8 are found for the free enzyme state and two pH independent Kcat/Km values of 4.1$\times$104 M-1 s-1 and 1.4$\times$104 M-1 s-1 at low and high pH, respectively. The profile is quite different in shape with other MMP family which has been reported, having broad pH optimum with two pKa values. The substrate specificity of SCD towards fluorescent heptapeptide substrates has been also examined by thin layer chromatography. The cleavage sites of the substrates have been identified using reverse-phase HPLC method.SCD cleaves Dns-PLA↓L↓WAR and Dns-PLA↓L↓FAR at two positions. However, the Dns-PLA↓LRAR, Dns-PLE↓LFAR, adn Dns-PLSar↓LFAR are cleaved exclusively at one bond. The double cleavages of Dns-PLALWAR and Dns-PLALFAR by SCD are in marked contrast to the close structurally related matrilysin. A notable feature of SCD catalysis agrees with the structural data that the S1' pocket of SCD is deeper than that of matriysin. The differences observed between SCD and matrilysin may form the basis of understanding the structural relationships and substrate specificities of the MMP family in vivo.

• Child Health Nursing Research
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• v.7 no.4
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• pp.483-493
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• 2001

This study is attempted to define risk factor of youth runaway impulse and to structure forecast model through an extensive analysis of the factors influencing the runaway impulse of youth. The subjects were 610 high school students in Seoul and Kyunggido. The collected data was analysed by SAS. The differences between the runaway impulse group and the non-runaway impulse group were subject to chi-square and t-test. Also logistic regression analysis was conducted on the basis of purposeful selection method for constructing the forecast model. The findings are as follows : the major predicting factors of youth runaway impulse are sex(odds ratio=1.886, p=.009), existence of friends of the opposit sex(odds ratio=2.011, p=.007), anti-social personality(odds ratio= 4.953, p=.000), depressive trend(odds ratio= 2.695, p=.000), family structure(odds ratio= 5.381, p=.000), marital relationship(odds ratio =1.893, p=.009) and also between parents and youth(odds ratio=3.877, p=.000), emotional abuse(odds ratio=1.963, p=.003), authoritative controlled rearing(odds ratio=2.135, p=.005) and stress from school(odds ratio=1.924, p=.008). Therefore, the forecast model will be contribute to the nursing intervention for prevention of runaway youth.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.428-432
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• 2015

Sixty-seven isolates of bacterial spot pathogen (Xanthomonas spp.) collected from six provinces of Korea were tested for the identification of their pathotypes and determination of their distribution throughout Korea in an effort to genetically manage the disease. Near isogenic lines of Early Calwonder (Capsicum annuum) pepper plants carrying $Bs_1$, $Bs_2$ and $Bs_3$, and PI235047 (C. pubescens) were used as differential hosts. Race P1 was found to be predominant, followed by race P7, and races P3 and P8 were also observed. This is the first report of races P7 and P8 in Korea. The races P7 and P8 were differentiated from the former races P1 and P3, respectively, on the basis of their ability to elicit hypersensitive reactions to PI235047.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.24 no.1
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• pp.55-60
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• 2011

In the present work, isogeometric analysis in linear elasticity problem is conducted using the basis functions from NURBS. The objectives of isogeometric analysis introduced is to integrate both geometric modeling(CAD) and computational analysis(CAE), and this can be accomplished from direct usage of geometric modeling by NURBS as the computational mesh. The merit of the isogeometry analysis is that NURBS surface are able to represent exact geometry from the control points and knot vectors, and also subsequent refinement is relatively simple relatively. In order to verify the computer codes developed in this study, it has been applied to two structural models of which geometry are simple ; 1) circular cylinder subjected to the constant internal pressure loading, 2) square plate with circular hole at center subjected to uniform tension. The exact solutions of these two models are available. Convergence of the approximate solutions by the present code for the isogeometry analysis are investigated by mesh refinement with inserting knots (h-refinement) and by mesh refinement with order elevation of the basis functions (p-refinement).

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.7
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• pp.976-986
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• 2015

The present study was conducted to determine the effects of lipid sources with different fatty acids profile on meat fatty acids profile and beef quality traits of Nellore. A total of 45 Nellore animals with an average initial body weight of $419{\pm}11kg$ (at $15{\pm}2mo$) were distributed in a completely randomized design consisting of 5 treatments and 9 replicates. The roughage feed was maize silage (600 g/kg on a dry matter [DM] basis) plus concentrate (400 g/kg on a DM basis). The dietary treatments were as follows: without fat (WF), palm oil (PO), linseed oil (LO), protected fat (PF), and soybean grains (SG). No effects of lipid sources were observed (p>0.05) on beef color, pH, water-holding capacity, and sarcomere length. Beef from cattle fed PO had greater shear-force values (p<0.05) compared to beef from cattle fed WF. Deposition of main unsaturated fatty acids (oleic, linoleic, and linolenic) was greater in treatments WF, SG, and LO, respectively, while the values of conjugated linoleic acid (CLA) were greater when animals were fed LO. The inclusion of LO in the diet enhances the concentration of CLA in longissimus muscle and subcutaneous fat besides improving the atherogenicity index and elongase activity. As such, LO can be used with the aim to improve the quality of beef from confined Nellore cattle. Conversely, the use of PO is not recommended since it may increase the concentration of undesirable unsaturated fatty acids in muscle and subcutaneous fat, shear-force and the atherogenicity index.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.546-553
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• 2017

Objective: An experiment was conducted to determine digestible energy (DE) and metabolizable energy (ME) of different byproduct feed ingredients fed to growing pigs, and to generate prediction equations for the DE and ME in feed ingredients. Methods: Twelve barrows with an initial mean body weight of 31.8 kg were individually housed in metabolism crates that were equipped with a feeder and a nipple drinker. A $12{\times}10$ incomplete Latin square design was employed with 12 dietary treatments, 10 periods, and 12 animals. A basal diet was prepared to mainly contain the corn and soybean meal (SBM). Eleven additional diets were formulated to contain 30% of each test ingredient. All diets contained the same proportion of corn:SBM ratio at 4.14:1. The difference procedure was used to calculate the DE and ME in experimental ingredients. The in vitro dry matter disappearance for each test ingredient was determined. Results: The DE and ME values in the SBM sources were greater (p<0.05) than those in other ingredients except high-protein distillers dried grains. However, DE and ME values in tapioca distillers dried grains (TDDG) were the lowest (p<0.05). The most suitable regression equations for the DE and ME concentrations (kcal/kg on the dry matter [DM] basis) in the test ingredients were: $DE=5,528-(156{\times}ash)-(32.4{\times}neutral\;detergent\;fiber\;[NDF])$ with root mean square error = 232, $R^2=0.958$, and p<0.001; $ME=5,243-(153 ash)-(30.7{\times}NDF)$ with root mean square error = 277, $R^2=0.936$, and p<0.001. All independent variables are in % on the DM basis. Conclusion: The energy concentrations were greater in the SBM sources and were the least in the TDDG. The ash and NDF concentrations can be used to estimate the energy concentrations in the byproducts from oil-extraction and distillation processes.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.20
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• pp.9021-9025
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• 2014

Background: Breast cancer (BC) is the most common cancer among females in Turkey. Predictors affecting the breast self-examination (BSE) performance vary in developing countries. Objective: To determine the frequency of BSE performance and predictors of self-reported BSEs among women in the capital city of Turkey. Materials and Methods: This cross-sectional study was conducted on 376 Turkish women using a self-administered questionnaire covering socio-demographic variables and BSE-related features. Results: Of the participants, 78.7% (N=296) reported practicing BSE, whereas 9.5% (N=28) were implementing BSE regularly on a monthly basis, and only 5.7% (N=17) were performing BSE regularly within a week after each menstrual cycle. Multivariate logistic regression modeling revealed that BSE performance was more likely in younger age groups [20-39 years] (p=0.018, OR=3.215) and [40-49 years] (p=0.009, OR=3.162), women having a family history of breast disease (p=0.038, OR=2.028), and housewives (p=0.013, OR=0.353). Conclusions: Although it appears that the rates of BSE performers are high, the number of women conducting appropriate BSE on a regular time interval basis is lower than expected. Younger age groups, family history of breast diseases and not being employed were identified as significant predictors of practicing BSE appropriately. Older age and employment were risk factors for not performing BSE in this sample.