• Journal of Embryo Transfer
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• v.15 no.1
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• pp.1-8
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• 2000

The ultrasound-guided oocytes cllection (ovum pick-up ; OPU) has become a substitution for superovlation in cattle. The objective of this study was to examine the effect of OPU frequency on the in vitro production of embryos in Hanwoo cattle. Six cycling Hanwoo cows were distributed into two groups for either once or twice weekly OPU sessions. Oocytes were collected by ultrasound-guided follicle aspiration(SA600) using a 6.5HMz transducer and attached with 18 gauge needle, with vacuum pressure of 40 mmHg. The cumulus-oocyte complexes (COCs) collected from each donor were matured in TCM 199 supplemented with 10% fetal bovine serum at 5% CO2 in air at 38.5$^{\circ}C$ for 22h and in vitro matured oocytes were co-incubated with sperm(separated by Percoll gradient) for 6h. The zygotes were co-cultured on cumulus cell monolayer in 10ul droplets in the same culture medium and conditions used for IVM for 7 days. On Day 7 of culture, development to blastocysts was examined. Although the number of oocytes collected was variable depending on individuals, overall embryo production in the twice per week OPU sessions was better that in the once per week sessions(6~21 vs 2~7 blastocysts produced, respectively). Two cows(E, A) were good oocyte donors and embryo production was superior in cow C ; however, cow F was a poor donor as compared to the others. In conclusion, these results suggest that for embryo production, twice weekly OPU sessions were better than once per week for producing embryos in vitro from Hanwoo cattle.

• Journal of Embryo Transfer
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• v.14 no.2
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• pp.121-129
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• 1999

The objective of this study was to produce calves by transfer of embryos derived from slaughter house(SHD) and ultrasound-guided ovum pick-up (OPU). At 60 hrs after injection of 400 mg FSH dissolved in 25% polyvinylpyrrolidone(PVP) by single dose, ultrasound-guided follicular oocyte aspiration was ferformed. Day-7 and day-8 blastocysts produced by in vitro maturation (IVM), fertilization (IVF) and culture(IVC) of the oocytes derived from SHD and OPU were nonsurgically transferred into recipients. The results obtained were as follows. The cleavage rate and the development rate to blastocysts were not significantly (P<0.05) different between the oocytes obtained by SHD (72.9% vs. 34.1%) and OPU (75.9% vs. 38.4%). The oocyte recovery rate from the number of follicles by ultrasound-guided aspiration were not significantly (P<0.05) different between Holstein (61.7%) and Hanwoo (60.1%), but the rate of oocytes useful for IVF was significantly (P<0.05) higher in Hanwoo (69.3%) than Holstein (59.6%). The cleavage rate and the development rate to blastocysts was not significantly (P<0.05) different between Holstein (74.9% vs. 39.2%) and recipients on day 8 of estrus cycle resulted in 13 pregnancies (34.2%). One of them was sacrificed during gestation period due to mastitis and another was aborted spontaneous. The resulting 14 calves were morphologically normal at birth. Seventy eight fresh OPU-IVF embryos were transferred into 21 recipients on day 8 of estrus cycles, resulting in pregnancy of 12 recipients (41.4%). Two of them were sacrificed during gestation period due to mastitis and the other two were aborted. Nevertheless, the 11 OPU-calves have been born normally.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.147-151
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• 2017

Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows. The aim of this present study was to compare the number of recovered oocytes and cultured In vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.1~62.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was $3.3{\pm}0.4$ cleaved embryo and $0.9{\pm}0.2$ blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P<0.05) higher than that in the other donors, However, blastocysts was similar (25.8~30.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium.

• Journal of Embryo Transfer
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• v.29 no.3
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• pp.265-271
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• 2014

Implementation of smart embryo technologies in cattle e.g. ovum pick-up followed by in vitro embryo production (OPU-IVP). Seasonal variation is important factor for follicular growth, oocytes quality, quantity and developmental competence. Therefore the aim of present study was carried out to investigated whether the seasons (hot and cool) effect on follicular development, oocyte recovery and subsequent embryo development. Follicular oocytes were aspirated from Korean native cows (Hanwoo) by the ovum pick-up (OPU) method, which was performed 24 times during two different seasons, the hot (July to September) and cool (October to December), from OPU donors. The recovered oocytes were classified according to morphological categories and used for in vitro embryo production (IVEP). The mean number of total follicles was significantly higher (p<0.05) during the hot season ($18.32{\pm}2.26$) compared to cool season ($15.41{\pm}3.34$). Furthermore, seasons did not significantly effect on the number of oocytes recovered (hot season: 41.16% vs. cool season: 46.14%). However, the average number of Grade A oocytes was significantly greater during hot ($1.75{\pm}1.86$) season compared to the cool season ($1.00{\pm}1.46$), but there was no significant difference of other grades oocytes. The cleavage rate (hot: 66.67% vs. cool: 63.3%) and embryo development (hot: 58.95% vs. cool: 56.97%) did not differ significantly between the seasons. In conclusion, the results of present study suggest that the season (hot and cool) does not have effects on the oocyte recovery and embryo developmental competence of in vitro cultured embryos.

• 한국경영정보학회:학술대회논문집
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• 2007.06a
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• pp.427-431
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• 2007

경제적 가치가 급증하고 있는 주파수자원은 유한하므로 이를 보다 효율적으로 배분하는 것이 전파관리의 중요한 과제가 되고 있다. 특히, 지나치게 높은 수준의 주파수 할당대가는 초기 투자비용을 증대시켜 경쟁력 있는 시장 형성과 원활한 서비스 제공을 저해하는 요소가 되며, 이에 따라 소요되는 비용은 소비자 요금으로 부당하게 부과될 수 있다. 따라서 본 연구에서는 주파수 이용대가를 국가별로 비교하여 현재 우리나라의 주파수 할당대가 수준을 분석하고자 한다. 세계적으로 주파수 할당대가의 산정방법은 IMT-2000의 사업자 선정을 계기로 크게 비교심사제도와 경매제도로 나누어 진행되어오고 있으며, 방법에 따라 주파수 이용대가의 결과는 다양하게 나타났다. 본 논문에서는 ITU, OVUM, OSIPTEL 자료를 바탕으로 약 30개 국의 IMT-2000 주파수 할당대가 산정 결과를 분석하고자 한다. 또한 주파수 할당대가 산정 시 영향을 미치는 요인을 관련 선행연구를 토대로 선정하여 회귀분석을 실시하고자 한다.

• Korean Journal of Veterinary Research
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• v.13 no.2
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• pp.111-118
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• 1973

The placentation in the golden hamster (Mesocricetus auratus) and the morphological changes occuring in its placenta from implantation to parturition have been observed by light microscope. The results obtained were summarized as follows. 1. The development of the placenta in the fertilized ovum implanted on the day 5 of gestation was described with increasing gestational age. 2. On the day 8 of gestation, the chorioallantoic placenta was formed by fusion of the allantois to the chorion of ectoplacental cone. 3. On the day 13 of gestation, Reichert's membrane with the parietal yolk sac disappeared in a circumscribed area antimesometrially.

• Electronics and Telecommunications Trends
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• v.28 no.5
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• pp.209-220
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• 2013

본 논문은 최근 중요성이 부각되고 있는 TD-LTE의 기술 특성과 주요 사업자의 네트워크 구축 및 서비스 추진동향에 대하여 분석 정리하였다. 기존에 발간된 문헌들이 주로 차이나 모바일 및 중국의 정책 동향에 초점을 맞추고 있는데 반해, 본 논문에서는 TD-LTE 사업자의 유형을 세분화하였고 각 유형별로 대표적인 사업자를 선정하여 네트워크 구축 및 서비스 추진동향을 고찰하였다. 본 논문에서 수행한, 정성적인 동향분석 결과에 따르면 TD-LTE가 전체 LTE시장에서 차지하는 비중은 2년전 Ovum, Pyramid사 등 전문기관들이 예측한 수치보다는 다소 낮을 것으로 판단된다. 또한, 국내 사업자의 경우 주로 트래픽 우회를 위한 네트워크로써 활용이 가능한데, 보다 유연한 정책적 접근이 요구된다고 사료된다.

• Korean Journal of Child Studies
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• v.7 no.1
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• pp.49-65
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• 1986

The purpose of this paper was the analysis of Buddhistic views of fertilization, abortion and birth referred to in the Code of Buddhism. The findings of my analysis of the allusions of the Code are as follows. 1. Modern science holds the view that in fertilization the spermatozoom and the ovum are the essential factors, while the Buddhistic view is that here must be the entry of a reincarnated soul ( 中有 ; jung-yu) into fertilization. 2. In Buddhism, fertilization is regarded as the giving of a new life and the receiving a life which consists of the sufferings, both physical and mental in nature, of the individual to be born. 3. Artificial abortion in any case is stictly prohibited because it is regarded as taking life. 4. Birth perse is assumed to consist of suffering for both the mother and the child itself. Therefore, these are analogous to the perinatoloty of modern science.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.6
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• pp.599-605
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• 1995

The ultrastructures of in vitro-derived bovine blastocysts have been compared with those of blastocysts obtained from a superovulated cow. In vivo blastocysts obtained from the uterus showed well-differentiated features, while in vitro-derived embryos, which were developed from in vitro fertilized ovum, showed insufficient cellular organizations. In vitro-derived embryos contained many undefined cellular organizations in the perivitelline spaces compared with in vivo-derived blastocysts. Other features of in vivo and in vitro blastocysts were characterized by differential development of microvilli projection into blastocoele from the surface of the trophoblast cells. The conceivable reason for the difference between in vivo and in vitro developments of bovine embryos is that it is likely that in vitro culture system adopted in the present experiment may not be sufficient for better embryonic development.

• Korean Journal of Veterinary Research
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• v.15 no.2
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• pp.233-240
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• 1975

Glycogen and alkaline phosphatase were studied histochemically in the placenta of the golden hamster (Mesocricetus auratus). The results were summarized as followings: 1. The histochemical distribution of glycogen and alkaline phosphatase was described in the various cells of both the placenta and uterus from implantation (5 days past coitum) parturition. 2. Glycogen appeared in the extraembryonic ectorm of the implanting ovum and increased gradually in the ectoplacental cone with the development of the placenta. 3. Glycogen granules began to accumulate in the labylinthine trophoblast of chorioallantoic placenta at 10.5 days and in the endodermal cells the visceral yolk sac sac at 10 days, respectively, and in these both cells glycogen increased gradually until the 13 days post coitum and after this time decreased progressively. 4. Alkaline phosphatase activity was high in the labyrinth, junctional zone and decidua, but was not observed in the yolk sac and trophospongium.