• Title/Summary/Keyword: outside experiment

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Anti-Parasitic Activity of Lespedeza cuneata Extract on Causative Agent of Nosemosis Type C, Nosema ceranae

  • Song, Hyunchan;Kim, Hyekyung;Kim, Ki-Young
    • Journal of Apiculture
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    • v.34 no.2
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    • pp.137-140
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    • 2019
  • Although honeybees (Apis mellifera) are crucial for maintenance of the ecosystem, population of honeybee has been steadily decreasing due to diseases including nosemosis. Nosemosis is a disease caused by Nosema ceranae and is now considered as a major threat to honeybees. N. ceranae is a microsporidian that stays in form of spore even before the infection, which makes it harder to control than other pathogens. People are now aware of this parasite, however, cure and preventive candidates for nosemosis are hardly found until today. In this study, in vitro experiment of Lespedeza cuneata treatment to prevent nosemosis were done using Trichoplusia ni cell line, BTI-TN5B1-4. Normal T. ni cells exhibited round shape without abnormal size. On the other hand, when N. ceranae were treated, cells deteriorated and some cells abnormally enlarged due to N. ceranae infection. Interestingly, treatment of T. ni cells with L. cuneate extract protected abnormal cell shape induced by N. ceranae infection to normal shape. Some N. ceranae spores were observed outside of the cells. Effective concentration range for N. ceranae control were experimented. Lowest concentration which can control nosemosis were 50 ㎍/mL. When the concentration of L. cuneata extract was exceeded 200 ㎍/mL, cytotoxicity started to show up.

Investigation on the Non-linear Injection Characteristics of GDI injector using 1D Simulation (1D 시뮬레이션 기반 GDI 인젝터의 비선형적 분사 특성 해석에 대한 연구)

  • Jinwoo Lee;Seoksu Moon;Donghan Hur;Jinsuk Kang
    • Journal of ILASS-Korea
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    • v.28 no.4
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    • pp.169-175
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    • 2023
  • Multi-injection scheme is being applied to GDI combustion to reduce PM and PN emission to meet the EU7 regulation. However, very short injection duration encounters the ballistic injection region, which injection quantity does not increase linearly with injection duration when applying multi-injection. In this study, numerical studies were conducted to reveal the cause of ballistic injection and the effect of design parameters on ballistic region using 1-D simulation, AMESim. Injection rate and injection quantity were compared with experiment to validate the established model, which showed the accuracy with 10% error. The model revealed that the tendency of ballistic region coincides with the needle motion behavior, which means that parameters at the upper part of needle such as electro-magnetic force, needle spring force and needle friction force have dominant effect on ballistic injection. To figure out the effect of electro-magnetic and needle friction force on ballistic, those parameters were varied to plus and minus 10% with model. The result showed that those parameters clearly changed the ballistic region characteristics, however, the impact became insignificant for outside of ballistic region, which means that the ballistic injection is mainly influenced by initial motion of injector needle.

Heating Effect by Electric Radiator in Greenhouse of Chrysanthemum Cultivation (전기 방열기가 국화재배온실의 난방에 미치는 영향)

  • Suh, Won-Myung;Leem, Jae-Woon;Kim, Young-Ju;Min, Young-Bong;Kim, Hyeon-Tae;Huh, Moo-Ryong;Yoon, Yong-Cheol
    • Journal of agriculture & life science
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    • v.44 no.4
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    • pp.79-85
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    • 2010
  • An analysis in heating effects of an electric radiator located in a 1-2W type chrysanthemum (3 cultivars) cultivation greenhouse installed in Gyeongsang National University drew the following conclusions. During the experiment period, the highest, average, and the lowest outside temperatures were in the ranges of $-3.8{\sim}21.3^{\circ}C$, $-5.2{\sim}16.1^{\circ}C$ and $-12.5{\sim}14.4^{\circ}C$, respectively, and the average relative humidity inside and outside the greenhouses were in the ranges of 43.5~98.6% and 35.2~100%, respectively. From mid-December to early February, the lowest outside temperature was recorded as approximately $-5.0{\sim}-10.0^{\circ}C$, which showed that it tended to be relatively lower than the temperatures recorded at the Jinju Meteorological Observatory. During the night, the leaf temperature measured directly under the radiator tended to be higher by $2{\sim}3^{\circ}C$ than that those at the middle point of the radiator, or higher by a negligible amount. In the case of root zone temperature, it was found that there was almost no difference between temperatures of the part directly under and the middle point, and the time when the highest temperature of root zone and other highest temperatures took place showed that there was about a 2-hour delay phenomenon. The total electricity consumption, energy supply and total heating cost during the experiment period were 2,800 kWh, 2,408,000 kcal and 112,000 won, respectively. When diesel, a kind of fossil fuel, was used as heating oil, the total heating cost was around 224,500 won. It was estimated that the total heating cost could be reduced by around 50% if a radiator was used.

Analysis on Field Applicability of SWAN Nested Model (SWAN Nested model의 현장 적용성 분석)

  • Kim, Kang-Min;Dae, Nam-Ki;Lee, Joong-Woo
    • Journal of Navigation and Port Research
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    • v.35 no.1
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    • pp.45-49
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    • 2011
  • The recent trend for numerical experiment requires more higher resolution and accuracy. Generally, in the wave field calculation, it starts with a large region formulation first and follows by a separated detailed region formulation by more denser grids for the main interest area considering the geographical and bathymetrical variation. The wave fields resulted from the large region calculation is being introduced into the detail region calculation as the incident waves. In this process there exists a problem of continuity. In order to get over such problem, method of variable gridding system or spectrum sampling, etc., is being used. However, it seems not enough to examine and analyze the related numerical errors. Therefore, it is investigated in this study the field applicability of the most pervasive use of wave model, the nested SWAN model. For this purpose, we made model experiment for two coastal harbours with different tidal environment, and compared and analyzed the result. From the analysis, it was found that both the extracted values, near the boundaries of the large and detail region and the nested formulation of SWAN model, show almost the same and no different between those with different tidal environment conditions. However it is necessary for reducing the numerical errors to set the boundaries for the detailed region outside of the rapid bathymetric change and deeper region.

THE EFFECT OF THE CITRIC ACID ON THE REPAIR OF THE DENUDED ROOTS TRANSPLANTED IN PERIODONTALLY INVOLVED EXTRACTION SOCKETS IN DOGS (성견 치주질환 발치와에 이식된 구연산 처리 치근의 치유에 대한 연구)

  • Chi, Jun-Soon;Kim, Chong-Kwan
    • Journal of Periodontal and Implant Science
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    • v.23 no.2
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    • pp.261-281
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    • 1993
  • The author transplanted periodontally-diseased teeth which had been treated with citric acid into a clinically healthy extraction sockets and periodontally-affected extraction sockets, and compared with the healing processes within these tissues. Recipient sites were prepared by surgically removing a part of alveolar bone of premolars of adults dogs, placing elastic orthodontic ligatures for 8weeks, thereby inducing periodontal disease. The diseased roots were extracted and transplanted into healthy extraction sockets, and these were designated as control group 1. Diseased roots transplanted into diseased sockets were designated as control group 2. Diseased roots which had been root planed, treated with citric acid and transplanted into healthy sockets were designated as experimental group 1, while identically treated roots which had been transplanted into diseased sockets were designated as experimental group 2. Observations were made at weeks 2, 8 and 12, with following results. 1. At week 2, experimental group 2 showed some inflammatory cell infiltration in the connective tissue above the extraction sockets, while control groups showed less inflammatory or foreign body reactions throughout the experiment. 2. In both control groups, root surface resorption was observed throughout the experiment, while experimental groups showed a little resorption. 3. Control group 1 & 2 showed ankylosis by newly-formed bone ground the resorbed root surfaces, while experimental group 1 & 2 displayed collagen fibers which are not functionally-arranged, with random, loose arrangement or parallel orientation to root surfaces, and newly-formed bone outside of them. 4. In both control groups & experimental groups which had been transplanted into a clinically healthy extraction sockets & periodontally affected extraction sockets groups, histological differences were not significant. 5. Root resorption or ankylosis in control group 1 & 2 had increased quantitatively as experiment progressed. 6. New bone formation developed from the base and lateral wall of extraction sockets. In both control groups & experimental groups, root surfaces lying next to the upper portion of extraction sockets showed little alveolar bone formation and surrounded by connective tissue fiber at weeks 2 & 8, while at weeks 12, they did show alveolar bone formation. 7. At week 12, experimental group 2 showed numerous cells which appeared to be periodontal ligament cells, with functionally arranged connective tissue fibers between the roots and alveolar bone.

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IPTV and User Scenario-Based Interface in Home Network Service (홈 네트워크 환경에서 사용자 중심 시나리오를 활용한 IPTV 인터페이스 분석)

  • Lee, Jee-Hee;Kim, So-Hyun;Kim, Hyun-Suk
    • The Journal of the Korea Contents Association
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    • v.10 no.3
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    • pp.92-100
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    • 2010
  • Due to the development of digital appliance, role of TV causes both-way by introducing IPTV, and SNS service causes big change of watching environment and residence environment. There are good conditions on the role of integrated control because it is arranged in the living room which secures movement most effectively and because family members can easily use, and the degree of use is high. Therefore, we infer user's needs by analyzing user scenario and current role of TV in home network environment. Primarily, we collect surveys of development scenario and technology which companies suggest TV applied by home network service, and secondly, we comparatively study scenario which the companies mentioned above suggest through observing user scenario, and study the role of IPTV in the future through actual scenario-based experiment by ethnography. After analyzing user scenario through case study and experiment, there are integrated device studies mainly in company study because it can be made up inside home, security and entertainment. On the other hand, there are patterns of user behavior by scenario experiment mainly in auto-tainment, security, and it showed that it is insufficient for interaction between TV and home media peripheral. Through this paper, we analyze context of home user, and based on this, we could suggest effective use of service development. Also after analyzing user form, we could know it also should be considered of ratio between activity inside home and activity outside home.

Influence of Air Temperature during the Growing Period on Water Core Occurrence in 'Hongro' Apple Cultivar and the Mitigation Technique (사과 '홍로' 품종의 생육기 기온이 밀증상 발생에 미치는 영향과 경감기술 연구)

  • Park, Moo-Yong;Song, Yang-Yik;Han, Hyun-Hee;SaGong, Dong-Hoon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.11 no.3
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    • pp.100-110
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    • 2009
  • 'Hongro' is one of the important mid-season apple cultivars bred in Korea. The recent occurrence of water core in Hongro and the consequent problems motivated this study. The objectives of our study were to investigate the influence of air temperature during the growing period on water core occurrence in Hongro and to provide methods to mitigate its impact. Based on our field experiments for three years, the results indicated that the occurrence of water core disorder was due to the prolonged exposure to high temperature ($T_a$) of >$30^{\circ}C$ during the ripening period. The rates of occurrence of water core disorder were higher in the fruit whose weight was more than 300g or those located outside the tree canopy and thus exposed to stronger solar radiation. In terms of mitigating the water core occurrence, the application of spraying $CaCl_2$ four times from late July to August was effective. Furthermore, between 1 and 15 August when the rate of occurrence was high with $T_a$ (from 3:00 to 5:00 p.m.) >$30^{\circ}C$, the micro-water sprinkling for 30 minutes starting at 6:00 p.m. with a supplementary spray conducted two hours after the first application drastically reduced the water core occurrence.

Micro-Spot Atmospheric Pressure Plasma Production for the Biomedical Applications

  • Hirata, T.;Tsutsui, C.;Yokoi, Y.;Sakatani, Y.;Mori, A.;Horii, A.;Yamamoto, T.;Taguchi, A.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.02a
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    • pp.44-45
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    • 2010
  • We are currently conducting studies on culturing and biocompatibility assessment of various cells such as neural stem cells and induced pluripotent stem cells(IPS cells) on carbon nanotube (CNT), on nerve regeneration electrodes, and on silicon wafers with a focus on developing nerve integrated CNT based bio devices for interfacing with living organisms, in order to develop brain-machine interfaces (BMI). In addition, we are carried out the chemical modification of carbon nanotube (mainly SWCNTs)-based bio-nanosensors by the plasma ion irradiation (plasma activation) method, and provide a characteristic evaluation of a bio-nanosensor using bovine serum albumin (BSA)/anti-BSA binding and oligonucleotide hybridization. On the other hand, the researches in the case of "novel plasma" have been widely conducted in the fields of chemistry, solid physics, and nanomaterial science. From the above-mentioned background, we are conducting basic experiments on direct irradiation of body tissues and cells using a micro-spot atmospheric pressure plasma source. The device is a coaxial structure having a tungsten wire installed inside a glass capillary, and a grounded ring electrode wrapped on the outside. The conditions of plasma generation are as follows: applied voltage: 5-9 kV, frequency: 1-3 kHz, helium (He) gas flow: 1-1.5 L/min, and plasma irradiation time: 1-300 sec. The experiment was conducted by preparing a culture medium containing mouse fibroblasts (NIH3T3) on a culture dish. A culture dish irradiated with plasma was introduced into a $CO_2$-incubator. The small animals used in the experiment involving plasma irradiation into living tissue were rat, rabbit, and pick and are deeply anesthetized with the gas anesthesia. According to the dependency of cell numbers against the plasma irradiation time, when only He gas was flowed, the growth of cells was inhibited as the floatation of cells caused by gas agitation inside the culture was promoted. On the other hand, there was no floatation of cells and healthy growth was observed when plasma was irradiated. Furthermore, in an experiment testing the effects of plasma irradiation on rats that were artificially given burn wounds, no evidence of electric shock injuries was found in the irradiated areas. In fact, the observed evidence of healing and improvements of the burn wounds suggested the presence of healing effects due to the growth factors in the tissues. Therefore, it appears that the interaction due to ion/radicalcollisions causes a substantial effect on the proliferation of growth factors such as epidermal growth factor (EGF), nerve growth factor (NGF), and transforming growth factor (TGF) that are present in the cells.

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APPLICATION OF WIFI-BASED INDOOR LOCATION MONITORING SYSTEM FOR LABOR TRACKING IN CONSTRUCTION SITE - A CASE STUDY in Guangzhou MTR

  • Sunkyu Woo;Seongsu Jeong;Esmond Mok;Linyuan Xia;Muwook Pyeon;Joon Heo
    • International conference on construction engineering and project management
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    • 2009.05a
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    • pp.869-875
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    • 2009
  • Safety is a big issue in the construction sites. For safe and secure management, tracking locations of construction resources such as labors, materials, machineries, vehicles and so on is important. The materials, machineries and vehicles could be controlled by computer, whereas the movement of labors does not have fixed pattern. So, the location and movement of labors need to be monitored continuously for safety. In general, Global Positioning System(GPS) is an opt solution to obtain the location information in outside environments. But it cannot be used for indoor locations as it requires a clear Line-Of-Sight(LOS) to satellites Therefore, indoor location monitoring system could be a convenient alternative for environments such as tunnel and indoor building construction sites. This paper presents a case study to investigate feasibility of Wi-Fi based indoor location monitoring system in construction site. The system is developed by using fingerprint map of gathering Received Signal Strength Indication(RSSI) from each Access Point(AP). The signal information is gathered by Radio Frequency Identification (RFID) tags, which are attached on a helmet of labors to track their locations, and is sent to server computer. Experiments were conducted in a shield tunnel construction site at Guangzhou, China. This study consists of three phases as follows: First, we have a tracking test in entrance area of tunnel construction site. This experiment was performed to find the effective geometry of APs installation. The geometry of APs installation was changed for finding effective locations, and the experiment was performed using one and more tags. Second, APs were separated into two groups, and they were connected with LAN cable in tunnel construction site. The purpose of this experiment was to check the validity of group separating strategy. One group was installed around the entrance and the other one was installed inside the tunnel. Finally, we installed the system inner area of tunnel, boring machine area, and checked the performance with varying conditions (the presence of obstacles such as train, worker, and so on). Accuracy of this study was calculated from the data, which was collected at some known points. Experimental results showed that WiFi-based indoor location system has a level of accuracy of a few meters in tunnel construction site. From the results, it is inferred that the location tracking system can track the approximate location of labors in the construction site. It is able to alert the labors when they are closer to dangerous zones like poisonous region or cave-in..

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An Evaluation of the Environmental Effects of Marine Cage Fish Farms: I. Estimation of Impact Region and Organic Carbon Cycling in Sediment Using Sediment Oxygen Consumption Rates and Macrozoobenthos (해상 어류가두리양식장의 환경영향평가: I. 퇴적물 산소소모율 및 저서동물을 이용한 유기물 오염영향권 추정 및 유기탄소 순환)

  • 이재성;정래홍;김기현;권정노;이원찬;이필용;구준호;최우정
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.9 no.1
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    • pp.30-39
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    • 2004
  • In order to understand the environmental impact of marine cage fish farms, we measured the vertical fluxes of particulate to the sediment, the distribution of organic carbon in core samples, sediment oxygen consumption rate (SOD), and macrobenthos with increasing distance from a fish cage in Miruk island located in Tongyong. The experiment was performed in August 2003. Measured values gradually decreased with distance, indicating that the organic matter in the sediment derived from the fish farm. The dominant macrobenthos species were Tharyx mulifilis, Lumbrineris longifolia, Sigambra tentaculata, and Capitella capitata, occupying 88% of the total population. Capirella capirata, an opportunistic polychaete species, were especially abundant between 0 to 5 m radius range. The estimated impact regions of organic matter enrichment based on sediment consilmption rates and compositions of macrobenthos were in good agreement. Most organic matter derived from the fish farm was deposited within a 10 m radius and then dispersed horizontally to nearby (at least 50 m) surface sediment. The vertical organic carbon fluxes to the sediment at the fish farm were higher by a factor of two than those outside the area. The remineralization organic carbon in the upper sediment layer was estimated to be 50% (1.07 g C m$^{-2}$ day$^{-1}$ ) at the fish farm. In contrast, outside the area, 30% (0.30 g C m$^{-2}$ day$^{-1}$ ) of organic carbon was recycled and the remaining 70% was deposited to the deep sediment layer.