• Title/Summary/Keyword: outgroup

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Molecular Phylogeny of Syngnathiformes Fishes (한국산 실고기목 어류의 분자계통)

  • Song, Choon-Bok
    • Korean Journal of Ichthyology
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    • v.21 no.sup1
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    • pp.75-75
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    • 2009
  • The previous morphology-based taxonomic frameworks within the family Sygnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study have beencarried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b and Rag2 nuclear DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Much of previous morphological analyses were supported by our molecular data, but some deep relationships were not clearly resolved with regard to members of the suborder Aulostomoidei.

Genetic Relationship in Chicken Breeds Using Molecular Co-ancestry Information

  • Ahlawat, S.P.S.;Vijh, R.K.;Mishra, Bina;Kumar, S.T. Bharani;Tantia, M.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.1
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    • pp.6-10
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    • 2008
  • Five chicken populations viz. Chittagong, Ghagus, Kalasthi, Kadaknath, Tellichery were genotyped using 25 highly polymorphic microsatellite loci. White leg horn was taken as an outgroup. To reveal the relationship and distinctiveness among five indigenous breeds various genetic distances based on molecular co-ancestry were estimated and multidimensional scaling was performed. The Ghagus and Kalasthi breeds were closely related and their separation was recent, whereas Chittagong had a remote ancestry with other indigenous chicken populations.

Systematic Relationship of the Anthozoan Orders Based on the Partial Nuclear 18S rDNA Sequences

  • Song, Jun-Im;Won, Jung-Hye
    • Animal cells and systems
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    • v.1 no.1
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    • pp.43-52
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    • 1997
  • Systematic relationship of 13 species representing seven orders of anthozoans, and one hydrozoan as an outgroup, were investigated. Distance and parsimony methods were used to analyze nucleotide sequence data obtained from the 18S rDNA. The inferred phylogeny indicates that two orders, Ceriantharia and Antipatharia within subclass Ceriantipatharia, are not closely related to each other. Instead, the former appears to be the most ancestral group among anthozoans but the latter is more closely related to order Actiniaria within subclass Zoantharia. The result also indicates that the actiniarian may not be a monophyletic group, because Epiactis Japonica is related more to order Scleractinia. Subclass Octocorallia appears to be a monophyletic group, within which order Pennatulacea diversed at first, and then orders Alcyonacea and Gorgonacea diversed later.

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External Morphology and Numerical Taxonomy among Habitat of Allium victorialis var. platyphyllum (산마늘의 자생지별 외부형태 및 수리분류학적 연구)

  • 유기억
    • Korean Journal of Plant Resources
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    • v.11 no.2
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    • pp.210-216
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    • 1998
  • Taxonomic studies in external morphology, principal component analysis and cluster analysis were conducted to understand the intraspecific relationship among three habitats (Jirisan, Odaesan and Ulleungdo) of allium voctorialis var. platyphyllum. External morphology such as bulb color, leaf blade length and width, petiole length, total leaf length, peduncle length, perianth lobe length and width, length of anther and filament were useful characters for identification of poplations in three habitats. The results obtained based on the principal component (Pc) analysis of treated 72 OTUs(included outgroup) were divided into three groups by the PC 1 ,2,3 and the sums of contributions for the total variance were 84.1%(PC1 51.0%, PC2 24.9% and PC3 8.2%, respectively). In cluster analysis by the UPGMA and Ward's methods , there was similarities in the compostion of clustered taxa, and only Ulleungdo population was distinctly identified from population of other two habitats.

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Molecular Phylogeny of Poecilostome Copepods Based on the 18S rDNA Sequences

  • Kim, Jihee;Kim, Won
    • Animal cells and systems
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    • v.4 no.3
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    • pp.257-261
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    • 2000
  • To elucidate phylogenetic relationships among poecilostome families 18S rDNA sequence data were generated for seven poecilostome and one cyclopoid copopods by PCR cloning and sequencing techmiques. Phylogenetic trees were constructed by maximum parsimony, neighbor joining, and maximum likelihood methods using cyclopoid sequence as an outgroup. The results from three different analyses showed that the seven poecilostome families were eiridel into two groups: Clausidiidae-Myicolidae-Synaptiphillidae-bomolochidae and Lichomologidae-Chondracanthidae-Ergasilidae. The molecular phylogenies were consistent with those from the morphological characters. Therefore, these analyses porvide further evidence for the utility of 18S rDNA sequences in addressing phylogenetic relationships among poecilostome families.

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Antecedents of Local Food Store Attitude Corresponding to the Power Concepts in Cultural Orientations (문화 지향성별 파워개념에 대응하는 로컬푸드점포에 대한 태도요인)

  • Choi, Nak-Hwan;Chong, Byoung-Hee;Lim, Ah-Young
    • Journal of Distribution Science
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    • v.14 no.8
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    • pp.129-138
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    • 2016
  • Purpose - The present study theoretically reviews the use of power norm according to the cultural orientation of consumers to categorize the shopping value that corresponds to the use of the power norm when consumers use local food stores. Research Design, Data, and Methodology - Based on the data collected from the survey to 130 undergraduates, salaried men and people at large, the group with cultural value orientation was divided further into the group of horizontal individualism and that of vertical collectivism by using cluster analysis of SPSS 18.0 program. And regression analysis of SPSS 18.0 was employed to verify the hypotheses. Results - The following conclusions were shown in the empirical study. First, in the group of vertical collectivism, contribution to regional profits and competitiveness, economic value and service superiority can positively affect the attitude to the local food stores while the social responsibility activity in the community can't. In the group of horizontal individualism, however, contribution to regional profits and competitiveness, social responsibility activity, economic value and service superiority were all found to positively affect the attitude to the stores. Second, the distinctive shopping value of the stores such as escapism, entertainment value and prestige does not affect the attitude to the stores. Third, the positive effects that the stores' level of responsibility for the outgroup community can exercise on the attitude to the stores turned out to be bigger in the group of horizontal individualism than in the group of vertical collectivism. Fourth, the impact that the degree of the stores' contribution to regional profits and competitiveness has on the attitude to the stores was found to be positive in both groups. However, no difference existed in the degree of positive effects between them. Conclusions - Marketing managers of local food stores should pay more attention to managing their contribution to practical shopping value and to interests as well as competitiveness of regional society, and to persuading consumers of the horizontal individualistic group by performing responsible activities for the outgroup of local society regardless of the types of consumers' cultural orientation.

Phylogenetic Relationships of Korean Viola (Violaceae) Based on matK and atpB-rbcL Sequence Data of Chloroplast DNA (엽록체 DNA의 matK와 aptB-rbcL 염기서열 분석에 의한 제비꽃속(Viola)의 계통유연관계)

  • Yoo, Ki-Oug;Jang, Su-Kil;Lee, Woo-Tchul
    • Korean Journal of Plant Taxonomy
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    • v.37 no.1
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    • pp.1-15
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    • 2007
  • Phylogenetic studies were conducted for 42 populations of Korean viola based on matK gene and atpB-rbcL intergenic spacer region of chloroplast DNA. In the matK tree, section Chamaemelanium and Dischidium were formed as a distinct group. Five subsections of section Nomimium were paraphyletic. In atpB-rbcL intergenic spacer region analysis, two species of sect. Chamaemelanium were monophyletic, and section Dischidium was placed sister to subsection patellares clade except for V. keiskei. Five subsections of section Nomimium were also paraphyletic as matK tree. the separate data analyses were incongruent in the relationships among 42 populations, especially for the position of section Dischidium and V. keiskei. The combined analyses of two chloroplast regions showed three major clades; section Chamaemelanium and Dischidium (x=6) formed a sister to subsections Hypocarpae and Trigonocarpae (x=10) clade; subsections Bilobatae and vaginatae (x=10 or 12) formed a clade with V. keiskei; and 19 populations of subsection patellares (x=12) except for V. keiskei were recognized as an independent clade within section Nomimium. Although combined data suggest three major clades of Korean viola, the origins of each clade from outgroup were discordance with previous ITS and trnL-F data.

Genetic Diversity of Pleurotus eringii Strains in Korea Based on Morphological Characteristics and PCR Polymorphism (형태적 특성과 PCR다형성 분석에 의한 국내 큰느타리버섯 계통의 유전적 다양성 분석)

  • Jeon, Sun-Jeong;Kim, Jong-Kun;Kim, Gum-Hee;Chi, Jeong-Hyun;Seo, Geon-Sik;Kang, Hee-Wan
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.19-27
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    • 2009
  • This study was conducted to investigate genetic characteristics of 25 Pleurotus eringii strains that have been released in Korea based on cultural, morphological features and PCR fingerprints. Strains PER-007 and PER-012 showed distinct cultural characteristics in growth rate, morphological characteristics of mycelial colony and fruiting bodies when compared to those of other strains. Strain PER-007 did not form primordium initiation in sawdust medium and PER-012 also showed different phenotypes on fruiting bodies. Eleven URP primers were used to detect PCR polymophic bands in P. eringii strains. Primers URP1F, URP2R, URP2F, URP4R, URP6R, URP9F and URP17R were selected as useful primers for amplifying PCR polymorphic bands in P. eringii strains. The genetic similarity index was calculated by using PCR polymorphic bands amplified by eight URP primers among the 25 strains. The P. eringii strains were grouped by four distinct clusters on the UPGMA analysis. The genetic similarity values ranged from 100% to 76% were observed in three major groups, suggesting close genetic relatedness of them. Exceptionally, PER-007 and PER-017 were involved in outgroup.

First Report of Asplenium yoshinagae (Aspleniaceae) from Korea and Its Phylogenetic Position Based on Morphology (미기록 가거꼬리고사리(꼬리고사리과)의 계통학적 위치)

  • Lee, Chang Shook;Kim, Jin Ohk;Lee, Nam Sook
    • Korean Journal of Plant Taxonomy
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    • v.38 no.2
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    • pp.79-91
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    • 2008
  • Asplenium yoshinagae (Aspleniaceae), previously known only from Japan, southwest China to Himalaya, was found in Gageo-do, Heuksan-myeon, Sinan-gun, Jeollanam-do. This species is similar to A. trichomonas, A. tripteropus, A. boreale, A. normale and A. oligophlebium by having gemmae and auricle of pinna, and distinguished from the latters by distinct stipe length, stalk of pinna, acute apex of pinna, length of indusium and shape of sorus. The Local name, Ga-geo-kko-ri-go-sa-ri, was newly given considering the locality. To reveal the interspecific relationships within the genus Asplenium in Korea, cladistic analysis was performed for 22 taxa of Asplenium as ingroup and 2 taxa of Diplazium as outgroup from Korea based on 20 morphological characters. As the results, the genus Asplenium seperated strongly from outgroup, and divided into 4 clades. Asplenium yoshinagae belong to the third clade. A. hondoense N. Murtakami & S. I. Hatanaka, which contained in the second clade, had treated as Hymenasplenium, but this results supported that this taxon may be contained in Asplenium, and also, Asplenium ruprechtii, not in Comptosorus. The morphological characters and illustrations of the species are provided together with photographs of habitat.

Phylogeny of Korean Viola based on ITS sequences (ITS 염기서열에 의한 한국산 제비꽃속(Viola)의 계통 유연관계)

  • Yoo, Ki-Oug;Jang, Su-Kil;Lee, Woo-Tchul
    • Korean Journal of Plant Taxonomy
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    • v.35 no.1
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    • pp.7-23
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    • 2005
  • Molecular phylogenetic studies were conducted to evaluate interspecific relationships in 40 populations of Viola including 35 Korean taxa, four Japanese populations and one outgroup using nuclear ribosomal ITS sequences. The phylogenetic analyses were conducted using parsimony and neighbor-joining methods. Subsection Trigonocarpae of section Nomimium appeared as the most basal clade within the Korean Viola. Section Dischidium and Chamaemelanium was monophyletidbootstrap 100%) and placed between subsect. Trigonocarpae and three other subsections of sect. Nomimium. Sect. Nomimium was paraphyletic. Although each subsectional grouping was in accordance with previous infrageneric classification based on morphological characters, yet discordance remained at the series level. Two evolutionary trends observed in the ITS tree were as follows. First, subsect. Trigonocarpae(x=10) was derived from the outgroup(x=6); Second, subsects. Bilobatae and Vaginatae(x=10 or 12), and subsect. Patellares(x=12) of sect. Nomimium were originated from sects. Dischidium and Chamaemelanium(x=6). Viola albida complex including three very closely related taxa was recognized as independent group within subsect. Patellares in parsimony tree. This result suggested that they should be treated as a taxa in series Pinnatae. Phylogenetic position of a putative hybrid species, Viola woosanensis was not supported with previous morphological hypothesis.