• Spatial Information Research
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• v.4 no.1
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• pp.93-105
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• 1996

The purpose of this study was to give some ideas to the improving direction and evaluation in order to solve physical' ecological problems appeared after urban park construction. This study selected Namsangol park as a site, because this park was constructed to the goal in order to rehabilitate the original landform as a part of "Namsan original landscape rehabilitation work". So this study was executed to investigate past and present landform change, soil condition, planting, water/climate etc., and then these were analyzed by GIS.- soil :pH, Ec, organic matter, the ability of moisture content, bulk density, porosity, etc.- planting:planting density, plant species- water/climate: the amount of rainfall, drainage and drainage-basin. And then this study overlayed soil+cut/fill, soil+pine tree, cut/fill + planting density, and cut/fill + plant species.t species.

• Proceedings of the Botanical Society of Korea Conference
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• 1998.07a
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• pp.10-18
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• 1998

For improvement of plants in disease resistance, it is most important to elucidate the mechanism to perceive and respond to the signal molecules of invaders. A model system with pea and its pathogen, Mycosphaerella pinodes, showed that the fungal elicitor induced defense responses in all plant species tested but that the suppressor of the fungus blocked or delayed the expression of defense responses and induced accessibility only in the host plant. In the world, many researchers believe that the pathogens` signals are recognized only on the receptors in the plasma membranes. Though we found that the ATPase and polyphosphoinositide metabolism in isolated plasma membranes responded to these fungal signals, we failed to detect specific actions of the suppressor in vitro on these plasma membrane functions. Recently, we found that ATPase (NTPases) and superoxide generating system in isolated cell wall were regulated by these fungal signals even in vitro, especially, by the suppressor in a strictly species-specific manner and also that the cell wall alone prepared an original defense system. The effects of both fungal signals on the isolated cell wall functions in vitro coincide perfectly with those on defense responses in vivo. In this treatise, we discuss the key role of the cell wall, which is plant-specific and the most exterior organelle, in determining host-parasite specificity and molecular target for improvement of plants.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.35-43
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• 2014

Objectives : Official Arisaematis Rhizoma is described only three species, Arisaema amurnse, Arisaema erubescens, and Arisaema heterophyllum, in national Pharmacopoeia. However, other Arisaema species, Arisaema ringens, Arisaema takesimense and Arisaema serratum, also have been distributed as an inauthentic Arisaematis Rhizoma in the herbal market. To develop a reliable molecular authentication method for Arisaematis Rhizoma in species level, we analyzed DNA barcode regions using six Arisaema species. Methods : Thirty-eight samples of six Arisaema plants species (A. amurense, A. amurense f. serratum, A. heterophyllum, A. takesimense, and A. serratum) were collected from different habitate and nucleotide sequences of DNA barcode regions (rDNA-ITS, matK, and rbcL gene) were analyzed after PCR amplification. The species-specific sequences and phylogenetic relations were estimated using entire sequences of three DNA barcodes based on the analysis of ClastalW and UPGMA, respectively. Results : The comparative analysis of DNA barcode sequences were revealed inter-species specific nucleotides to distinguish the medicinal plant of Arisaema Rhizoma in species levels excluding between A. amurense and its subspecies (A. amurense f. serratum) and A. takesimense and A. serratum, respectively. However, we obtained sequence differences enough to discriminate authentic and inauthentic Arisaematis Rhizoma. Therefore, we suggest that these SNP type molecular genetic markers were an reliable method avaliable to identify official herbal medicines. Conclusions : These marker nucleotides could be useful to identify the official herbal medicines by providing definitive information that can identify original medicinal plant and distinguish from inauthentic adulterants and substitutes.

• Journal of Species Research
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• v.2 no.2
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• pp.203-222
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• 2013

The district of Ganghwa-gun in the western coast (Seohae) of the Korea is consisted of several islands, Ganghwa-do, Seokmo-do, Gyodong-do, Jumun-do, Boreum-do, Seogeom-do and Donggeom-do. Since several islands of them were connected to mainland of Korea by bridges, the islands attract a lot of tourists from Seoul, Incheon and Gyeonggi-do areas. Construction of new roads, pensions, resort areas and restaurants provided economical benefits to local residents while natural vegetation of these islands has deteriorated continuously. Before biodiversity of vascular plants in these regions is damaged seriously by human impacts, it is needed to conserve original vegetation of the islands for restoration and monitoring in the future. Therefore, the primary purpose of the study is to clarify the flora of Donggeom-do, belonging to Ganghwa-gun. From the 15 times of field trips to the island, we have collected a number of vascular plants, 512 taxa in total, including 110 families, 329 genera, 449 species, six subspecies, 52 varieties, four forms and one hybrid. Of 512 taxa investigated, we have identified 12 taxa to be endemic to Korea. Six taxa were endangered or rare species which were designated by the Ministry of Environment and Korea Forest Service, respectively. 25 taxa were recognized as floristic regional indicator species designated specially by the Ministry of Environment. Seven species have shown their northern or southern distributional limit ranges, and 29 taxa were recognized as halophytes. The naturalized plants were identified as 55 taxa, and their naturalization ratio was 10.7%.

• Korean Journal of Plant Taxonomy
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• v.45 no.2
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• pp.136-144
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• 2015

We reviewed the taxonomic identity and morphological characteristics of Cardamine manshurica (Kom.) Nakai based on its original description and type specimens. Cardamine manshurica, with its smaller flowers and siliques and earlier flowering season, is distinguished from the closely related species C. flexuosa With. This species-level difference is additionally supported in a phylogenetic tree constructed using nrDNA ITS sequences. Taken together, we therefore propose that C. manshurica is a distinct species. The scientific name 'Cardamine manshurica (Kom.) Nakai' is accepted.

• The Korea Journal of Herbology
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• v.33 no.4
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• pp.43-51
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• 2018

Objectives : Pharbitidis Semen, the seeds of Ipomoea nil (L.) Roth or I. purpurea (L.) Roth, is well-known traditional herbal medicine in Korea. But it is often marketed as a different seed or mixtures of its closely related species. Thus, the present study aims to provide external and micromorphological characters and identification key by using stereoscope (ST) and scanning electron microscope (SEM) for discriminating authentic of Pharbitidis Semen. Methods : A discrimination on external morphological characteristics of sepals, fruits, seeds, and hilum, testa cell micromorphology in the original plants and its congeneric species was carried out using digital calipers, ST, and SEM. Results : Number of valves (degree of apex of each valve), number of seeds per locule, hairy in capsules and size, luster, density of hairy, hilum shape in seeds and shape of cell, anticlinal, periclinal wall in testa may have high discriminative value. The seeds of Ipomoea nil as an original plant of Pharbitidis Semen were distinguished from other species by the relative larger in size, ovoid-trigonous in shape, mostly flabellate or triangular to trapezoid in outline (c.s.), dull, and puberulent in surface and thicken anticlinal wall. Conclusions : On the basis of the results, an identification key of Pharbitidis Semen and closely related species is provided. Our observations suggest that the combination of morphological characters and other studied results could be helpful in the successfully identified authentic herbal medicines. Moreover, micromorphological characters using SEM could be useful for discriminating authentic medicines.

• Korean Journal of Plant Taxonomy
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• v.46 no.3
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• pp.295-300
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• 2016

Sisyrinchium micranthum Cav., a newly naturalized species, was found in Seogwipo, Jeju-do. This species is native to Central and South America and is reported as a widely invasive species around the world. Sisyrinchium micranthum is a sister species of S. rosulatum E. P. Bicknell (Deung-Sim-But-Kkot in Korean), reported as an invasive species earlier in Korea, and both species are members of the subgenus Echthronema. Sisyrinchium micranthum is distinguished from S. rosulatum by the urceolate shape of its flowers and by the large size of its fruits and seeds. Its local name is "Yeon-Deung-Sim-But-Kkot" based on the light blue corolla. We also found that Sisyrinchium angustifolium Mill., previously used for Deung-Sim-But-Kkot, was misapplied. It belongs to the subgenus Sisyrinchium and is morphologically very different from S. rosulatum. We corrected the scientific name of Deung-Sim-But-Kkot by changing it from S. angustifolium to S. rosulatum based on the type specimens, original descriptions, and recent studies.

• The Korea Journal of Herbology
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• v.34 no.5
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• pp.1-12
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• 2019

Objectives : The consumption of licorice is large in Korea, but domestic production is insufficient due to the lack of adaptability. This study aimed to provide a morphological basis for adding interspecific hybrid licorice with improved adaptability to pharmacopoeia. Methods : This study was to establish identification criteria for the original plants, external and internal morphology of the authentic herbal medicines (Glycyrrhiza uralensis, G. glabra and G. inflata), market products and artificially interspecific hybrid forms of licorice. For this purpose, previous studies were investigated and visual and histological observations were carried out. We focused on the internal morphology by microscopic observation for securing objectivity. Finally, we proposed the identification keys for precise classification of each part. Results : 1) Original plant : Licorice species in the compendium were distinguished by the number of leaflets, presence of hair on the fruit, curvature and swelling of the fruit. 2) External morphology : Licorice species were distinguished by degree of powderiness, tearing gap, radial structure in the cross section and existence of protrusion of outer epidermis. 3) Internal morphology : Licorice species were distinguished by the degree of development of phloem fiber bundle, development of obliterated sieve, whether the secondary medullary ray are branched. In the case of interspecific hybrids, the characteristics of both species used for hybridization were mixed in all observation methods. Conclusions : These results suggest that the interspecific crossbred licorice is suitable for the pharmacopoeial standard. Therefore, it can be applied as a herbal medicine through additional supplementary study.

• The Korea Journal of Herbology
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• v.25 no.4
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• pp.47-54
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• 2010

Objectives : The original plant species of Schisandrae Fructus (O-mi-ja) is prescribed as Schisandra chinensis $B_{AILL.}$, in Korea, but S. chinensis $B_{AILL.}$ and S. sphenanthera $R_{EHD.}$ et $W_{ILS.}$ in China. Moreover, fruit of several other species in genus Schisandra also have been used as the same herbal medicines. To develop a reliable method for correct identification of Schisandrae Fructus and to evaluate the phylogenetic relationship of S. chinensis and its related species, we analyzed internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA). Methods : Twenty-four plant samples of three Schisandra species and one Kadsura species, S. chinensis $B_{AILL.}$, S. spenanthera $R_{EHD.}$ et $W_{ILS.}$, S. nigra $M_{ax.}$ and Kadsura japonica $D_{UNAL}$ were collected from each different native habitate and farm in Korea and China. The nrDNA-ITS region of each samples were amplified using ITS1 and ITS4 primer and nucleotide sequences were determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW based on the entire nrDNA-ITS sequence. Results : In comparative analysis of the nrDNA-ITS sequences, we found specific nucleotide sequences including indels (insertions and deletions) and substitutions to distinguish C. chinensis, S. spenanthera, S. nigra, and K. japonica. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origin of O-mi-ja. Moreover, we evaluated the phylogenetic relationship of four plant species by the analysis of nrDNA-ITS sequences. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterants for O-mi-ja.

• Journal of Ecology and Environment
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• v.30 no.3
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• pp.231-236
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• 2007

This study examined the decomposition of blades and culms of aquatic emergent plant species, Zizania latifolia, Phragmites communis and Typha angustata, and changes in nutrient contents during decomposition. Z. latifolia, P. communis and T. angustata were the most frequently occurring species in Lake Paldang of Han River, Korea. Experiments were carried out from July 27 to December 14, 2005 in Lake Paldang using the litter bag method. The remaining masses of blade litter of each species at the end of experimental period were 21.2% of initial dry weight in Z. latifolia, 32.5% in P. communis, and 44.7% in T. angustata. In addition, the remaining mass of culm was 22.6% of initial dry mass in Z. latifolia, 56.4% in P. communis, and 38.1% in T. angustata. During the litter decomposition period, P, K, Na, and Mg concentration decreased rapidly within 10 days, but Ca and Mg concentration declined slowly. K contents remained below 10% of initial values in all litter samples retrieved during decomposition, whereas Ca and Mg concentration remained above 40% and 50% during decomposition in all three species. Na, P and Mn contents in litter varied among species and plant parts. P concentration in culms of P. communis remained at about 60% of initial concentration throughout the study, but the remaining P content in culms of Z. latifolia was only 10% of the original value at the end of the study period. The Mn concentration in blades of P. communis increased about 15-fold relative to the initial content by the end of experiment.