• Journal of Families and Better Life
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• v.27 no.4
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• pp.177-188
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• 2009

The purpose of this study was to examine the effects of family climate of fathers' family of origin, fathers' satisfaction with marriage and life, and parenting on father-son intimacy. A total of 297 adolescents(M=15 yrs. SD=1.4) and their fathers completed questionnaires. Data were analyzed by structural equation model using AMOS 7.0. Results indicated that 1) family climate of fathers' family of origin affected further-son intimacy positively through both fathers' high marital and life satisfaction, and thereby supportive parenting behavior. 2) Family climate of the family of origin affected farther-son intimacy negatively through low marital satisfaction and thereby high psychological controlling parenting. These results evinced that family climate of father's family of origin could be the source of father-son intimacy while fathers' marital and life satisfaction and parenting behaviors mediate the relationships.

• Journal of Families and Better Life
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• v.14 no.3
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• pp.151-166
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• 1996

This study examined the effects of social support on the perceived life satisfaction of middle-aged wives. To better understand the effects of social support on the perceived life satisfaction of middle-aged wives this study considered the following four different types of social support: emotional material informational and companionshp support. In addition each type of social support from the following five sources-the family of origin network the family of husband network friends neighbors and social organization-were examined separately in relation to the perceived life satisfaction of middle-aged wives. The data for his study were collected in the winter of 1995 from 417 housewives in Pusam wholse youngest child has graduated from elementary schools. Analyses of these data proved that (1) contrary to the classical "middle-age cristis" theory the average level of the perceived life satisfaction of middle-aged wives is relatively high; (2) there is a strong positive correla ion between the global level of social support and the overall life satisfaction of middle-aged wives; (3) the family of origin network is the most important provider of social support to middle-aged wives; and (4) the effects of social support on the perceived life satisfaction of middle-aged wives differ across types and sources of support. Findings showed that emotional informational and companionship support from the family of origin network have significantly positive effects on the perceived life satisfaction of middle-aged wives. On he other hand the material support from the family of origin network proved to have little effect.le effect.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.4
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• pp.650-657
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• 2009

This study was designed to determine the effects of meat country-of-origin on the perceived quality, perceived risk, perceived value, and purchase intension of restaurant menu items. A total of 237 university students in Seoul were used in the final statistical analyses, which included factor analysis, ANOVA and Duncan's test. The results were as follows: First, in the case of perceived quality, the participants gave higher evaluations of restaurant menu items better when the origin of the foodstuff was Korea. Second, the effects of origin on perceived value were not statistically significant. Third, the effect of origin on the purchase intension of fried chicken was statistically significant, but the effects of origin on the purchase intension of boned rib and fillet were not statistically significant. Lastly, the limitations of this study and suggestions for further research are presented.

• International Journal of Industrial Entomology and Biomaterials
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• v.11 no.2
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• pp.125-127
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• 2005

In order to improve the transformation efficiency of the Bacillus thuringiensis (Bt)-Escherichia coli (E. coli) shuttle vector, pHT3101, we intended to minimize replication origin of Bt in pHT3101. For this, two modified shuttle vectors, pHT1K and pHT261, in which 2.9 kb of replication origin of Bt were shortened to 1 kb and 261 bp, respectively as previously reported. Whereas the pHT1K could efficiently transform Bt into the antibiotic resistant, no transformants were obtained with pHT261. Furthermore, pHT1K showed higher transformation efficiency compared to that of parent vector, pHT3101. Therefore, pHT1K might be a very useful Bt-E. coli shuttle vector carrying minimal replication origin of Bt.

• Animal Bioscience
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• v.34 no.6
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• pp.941-948
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• 2021

Objective: In Japan, approximately 50 breeds of indigenous domestic chicken, called Japanese native chickens (JNCs), have been developed. JNCs gradually became established based on three major original groups, "Jidori", "Shoukoku", and "Shamo". Tosa-Jidori is a breed of Jidori, and archival records as well as its morphologically primitive characters suggest an ancient origin. Although Jidori is thought to have been introduced from East Asia, a previous study based on mitochondrial D-loop sequences demonstrated that Tosa-Jidori belongs to haplogroup D, which is abundant in Southeast Asia but rare in other regions, and a Southeast Asian origin for Tosa-Jidori was therefore suggested. The relatively small size of the D-loop region offers limited resolution in comparison with mitogenome phylogeny. This study was conducted to determine the phylogenetic position of the Tosa-Jidori breed based on complete mitochondrial D-loop and mitogenome sequences, and to clarify its evolutionary relationships, possible maternal origin and routes of introduction into Japan. Methods: Maximum likelihood and parsimony trees were based on 133 chickens and consisted of 86 mitogenome sequences as well as 47 D-loop sequences. Results: This is the first report of the complete mitogenome not only for the Tosa-Jidori breed, but also for a member of one of the three major original groups of JNCs. Our phylogenetic analysis based on D-loop and mitogenome sequences suggests that Tosa-Jidori individuals characterized in this study belong to the haplogroup D as well as the sub-haplogroup E1. Conclusion: The sub-haplogroup E1 is relatively common in East Asia, and so although the Southeast Asian origin hypothesis cannot be rejected, East Asia is another possible origin of Tosa-Jidori. This study highlights the complicated origin and breeding history of Tosa-Jidori and other JNC breeds.

• Journal of Microbiology and Biotechnology
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• v.17 no.11
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• pp.1841-1847
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• 2007

We previously identified the origin of replication of p703/5, a small cryptic plasmid from the KBL703 strain of Enterococcus faecalis. The origin of replication contains putative regulatory cis-elements required for replication and a replication initiator (RepA) gene. The replicon of p703/5 is similar in its structural organization to theta-type plasmids, and RepA is homologous to a family of Rep proteins identified in several plasmids from Gram-positive bacteria. Here, we report molecular interactions between RepA and the replication origin of p703/5. DNase I footprinting using recombinant RepA together with electrophoretic mobility shift assays confirmed the binding of RepA to the replication origin of p703/5 via iterons and an inverted repeat. We also demonstrated the formation of RepA dimers and the different binding of RepA to the iteron and the inverted repeat using gel filtration chromatographic analysis, a chemical crosslinking assay, and electrophoretic mobility shift assays in the presence of guanidine hydrochloride. Our results suggest that RepA plays a regulatory role in the replication of the enterococcal plasmid p703/5 via mechanisms similar to those of typical iteroncarrying theta-type plasmids.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.4
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• pp.795-802
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• 2007

The fire not to back to its origin(火不歸原) is said that source yang(元陽) of sea of qi(氣海) rises because fire(火) of lower energizer(下焦) can't return to its origin. Successive medical men regarded the cause of it as yang deficiency(陽虛) or yin deficiency(陰虛) generally, but Jangseoksun(張錫純) presented eight kinds of cause, they are syndrome of upcast yang(戴陽證), deficiency of qi(氣虛), yin deficiency, yin and yang deficiency(陰陽虛), thoroughfare qi ascending counterflow(衝氣上衝), heart fire(心火), yang deficiency with cold fluid retention(寒飮) in middle energizer(中焦寒飮), yang deficiency with sunken cold locked in(沈寒錮冷). The method of conducting fire back to its origin may be the treatment of fire not to back to its origin as an interpretation of the phrase in a broad sense, but it is limited to yang deficiency with sunken cold locked in besides syndrome of upcast yang as the treatment based on pathological conditions. By this standpoint Eunsuryong(殷壽龍) used conducting fire back to its origin to remove hidden cold(伏寒) and make rising false fire(假火) settle. The meaning of conducting fire back to its origin is not just raise yang qi(陽氣) but break sunken cold locked in by using the drugs like Buja(附子), Yukgye(肉桂). Jakyak(芍藥) can concentrate yang qi on the life gate(命門) by converging it, Sukjihwang(熟地黃) can supply yin essence(陰精) and check the intense nature of tonifing yang(補陽) drugs. So if we want to use the method of conducting fire back to its origin, we should confirm the symptoms of sunken cold locked in and yang deficiency not to misdiagnose yin deficiency.

• Journal of Families and Better Life
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• v.26 no.2
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• pp.129-141
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• 2008

The purpose of this study was to investigate the effect of family-of-origin experiences on marital satisfaction and psychological adjustment among married men and women. For this purpose, the data were collected from 123 married men and 188 married women using self-administered questionnaire method by AMOS 7.0 program. The results of the study were as follows : First, men and women's family-of-origin experience(family of origin health, parents' marital conflict, triangulation) had a direct effect on marital satisfaction. Secondly, men's family-of-origin experience had a direct effect on psychological adjustment(self-esteem, depression). But women's family-of-origin experience had a indirect effect on psychological adjustment. Finally, men and women's marital satisfaction had a direct effect on psychological adjustment.

• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1234-1240
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• 2015

Heat shock RNA 1 (HSR1) is described as a "eukaryotic heat-sensing noncoding RNA" that regulates heat shock response in human and other eukaryotic cells. Highly conserved HSR1 sequences have been identified from humans, hamsters, Drosophila, Caenorhabditis elegans, and Arabidopsis. In a previous study, however, it was suggested that HSR1 had originated from a bacterial genome. HSR1 showed no detectible nucleotide sequence similarity to any eukaryotic sequences but harbored a protein coding region that showed amino-acid sequence similarity to bacterial voltage-gated chloride channel proteins. The bacterial origin of HSR1 was not convincible because the nucleotide sequence similarity was marginal. In this study, we have found that a genomic contig sequence of Comamonas testosteroni strain JL14 contained a sequence virtually identical to that of HSR1, decisively confirming the bacterial origin of HSR1. Thus, HSR1 is an exogenous RNA, which can ectopically trigger heat shock response in eukaryotes. Therefore, it is no longer appropriate to cite HSR1 as a "eukaryotic functional noncoding RNA."

• Journal of agriculture & life science
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• v.43 no.5
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• pp.57-62
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• 2009

This study was conducted to analyze to the applicability of refined saps of Acer mono, A. mono for. rubripes, A. okamotoanum through sap refining system. 1 species of Bacillus genus, 3-4 species of yeast and 2 species of fungi were detected in the origin sap of three Acer spp. The pH in the origin sap were 6.5, and decreased in refining sap as 6.3-6.4. Sucrose in sugar components was detected in the origin and refining sap of Acer spp., but glucose and fructose were not detected. Compared the origin and refining sap, mineral components decreased slightly in refining sap. These results indicate that refining sap of Acer spp. are drinkable with long-term storage.