• Journal of Ginseng Research
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• 제27권3호
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• pp.93-97
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• 2003

Alternative or complementary medicine plays an important role in health care system. Ginseng, being one of the most popular oriental herbs, is believed to contain various steroid hormone activity. Ginseng has been demonstrated pharmacological effect in the cardiovascular, endocrine, central nervous, and immune system. Our objective was to study that total saponin might mediate some of their actions by binding to the steroid hormone receptor, as they share many of the actions of steroid hormone in various physiological system. Using total saponin from Panax Ginseng, we have studied the possibility of total saponin being a potential estrogen receptor, androgen receptor, and retinoic acid receptor ligand. Total saponin activated the transcription of both the estrogen and androgen responsive luciferase reporter plasmids at a concentration of 100$\mu\textrm{g}$/ml in COS cells transiently transfected with the corresponding receptor and hormone responsive receptor plasmids. And total saponin caused a concentration-dependent stimulation of estrogen receptor. Total saponin increased the expression of estrogen responsive c-fos proto-oncogene at the protein level in MCF7 cells at 24 h treatment as examined by Western analysis. The c-fos induction was used as a specific marker of estrogen responsiveness. This activation was inhibited by the specific estrogen receptor antagonist, ICI 182,780. However, total saponin failed to activate the retinoic acid receptor in COS cells transiently transfected with the corresponding receptor and retinoic acid responsive reporter plasmids. These results show that total saponin is capable of activating estrogen and androgen receptors.

• 대한한의학방제학회지
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• 제20권1호
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• pp.123-132
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• 2012

Objectives : The purpose of this study is to find out herb-pair prototype composed of Zanthoxyli Fructus, to examine compatibility regulation between them and to classify them into seven features of compatibility. Methods : To search usages of Zanthoxyli Fructus compatibility, we have investigated the classics of herb and prescription such as "五十二病方"(Fifty-two kinds of Diseases), "黃帝內經"(The Medical Classic of Yellow Emperor), "傷寒論"(Treatise on Febrile Caused by Cold), "金匱要略"(Synopsis of Prescriptions of The Golden), "附廣肘后方"(Handbook of Prescriptions for Emergencies), "本草綱目"(Compendium of Materia Medica), "得配本草"(Deukbae Materia Medica). Results : In these books Zanthoxyli Fructus was paired with below herbs; 桂(Cinnamomi Cortex), 薑(Zingiberis Rhizoma), 吳茱萸(Evodiae Fructus), 細辛(Asari Radix), 茴香(Foeniculi Fructus), 肉荳蔲(Cistanches Herba), 釜下土(Terba Flava Usta), 益智仁(Alpinae Oxyphyllae Fructus), 飴(Saccharum Granorum), 葱白(Allii Radix), 梨(Pyrus), 牙皂(Gleditsia Fructus), 杏仁(Armeniacae Amarum Semen), 蕎麥粉(Fagopyrum esculentum Moench), 胡粉(Minium), 白朮(Atractylodis Rhizoma White), 蒼朮(Atractylodis Rhizoma), 麻子仁(Cannabis Fructus), 大麥蘗(Hordei Fructus Germiniatus), 麴(Massa Medicata Fermentata), 生地黃(Rehmanniae Radix), 茯苓(Poria), 牡蛎(Ostreae Concha), 白礬(Alumen), 雄黃(Realgar), 烏頭(Aconiti Radix), 附子(Aconiti Lateralis Preparata Radix). Conclusions : Zanthoxyli Fructus herb-pair classified by seven features of compatibility are as follows; 1. Mutual reinforcement compatibility with : Cinnamomi Cortex, Zingiberis Rhizoma, Evodiae Fructus, Asari Radix, Foeniculi Fructus, Cistanches Herba, Terba Flava Usta, Alpinae Oxyphyllae Fructus, Saccharum Granorum, Allii Radix, Pyrus, Gleditsia Fructus. 2. Mutual assistance compatibility with : Armeniacae Amarum Semen, Fagopyrum esculentum Moench, Minium, Atractylodis Rhizoma White, Atractylodis Rhizoma, Cannabis Fructus, Hordei Fructus Germiniatus, Massa Medicata Fermentata, Rehmanniae Radix, Poria, Ostreae Concha, Alumen. 3. Mutual restraint compatibility with: Realgar, Aconiti Radix, Aconiti Lateralis Preparata Radix) 4. Mutual detoxication compatibility with: 桑汁(Juice of Mori Folium), 桑根汁(Juice of Morus Cortex), 大蒜(Allii Bulbus), 薺苨(Remotiflori Radix), 桂汁(Juice of Cinnamomi Cortex).

• 대한한의학원전학회지
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• 제29권4호
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• pp.37-52
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• 2016

Objectives : The paper seeks to delve into a study of smallpox, specifically as one of the most representative contagious diseases during the Chosun era and how it was treated during that time. The lack of traditional medical texts that deal with smallpox has led this paper to dive into Duchanggyeongheombang. Methods : Everything related to edition and copy, and the author was organized based on pre-existing studies. The copy that is assumed as the original copy was considered and studied. The book was compared to and reviewed with Donguibogam, and the differences between the book and Korean translation were given attention as well. Results : Park Jin-hee is assumed to have authored Duchanggyeongheombang, and it is like that was had access to texts published by the state, one of which includes Donguibogam. He utilized the periods with smallpox outbreaks as his frame of study and put together a series of treatment for different symptoms. To this, he attached various medical charts, and added Korean translation for higher usability. In his book, he placed emphasis on spleen and stomach, but when needed, he did not shy away from using cold and coolness herbs. Conclusions : Duchanggyeongheombang was influenced partially by Donguibogam, but the book was given new life through the author Park Jin-hee's edition. Medical charts were added to increase its usability, and Korean translation was provided for wider audience. Its scope of prescription is very wide, and it provided the reasonings behind its medical judgements based on actual clinical studies instead of being tied down to traditions or taboos.

• 대한한방소아과학회지
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• 제32권3호
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• pp.26-43
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• 2018

Objectives The purpose of this study was to analyze recent traditional Chinese medicine clinical studies for treatment of childhood obesity. Methods We analyzed 11 clinical studies from January, 2000 to March, 2018 about childhood obesity from the China Academic Journal (CAJ) and China National Knowledge Infrastructure (CNKI). All selected studies were Randomized Controlled Trials and analyzed in order of publication year, demographic information, treatment method, evaluation methods, results, adverse reactions and relapse after treatment discontinuation. Results The main Traditional Chinese Medicine (TCM) treatment was the herbal decoctions, granules and pills. In most studies, the total efficacy of the treatment group was reported to be higher than that of the control group. Adverse reactions were found in small number of studies. Frequently used medical herbs were Crataegi Fructus (山?), Atractylodis Rhizoma (蒼朮), Citri Pericarpium (陳皮), Poria (茯?), and Nelumbinis Folium (荷葉). Also, Water-draining and swelling-dispersing medicinal (利水退腫藥), Qi-regulating medicinal (理氣藥), Resolving dampness with aroma medicinal (芳香化濕藥), Blood-activating and stasis-dispelling medicinal (活血祛瘀藥), Qi-tonifying medicinal (補氣藥), and Digestant medicinal (消食藥) were commonly used. Conclusions This study showed that traditional Chinese medicine, especially herbal medicine treatment can be effective and safe option for treating childhood obesity. These research results can be utilized in other clinical studies as well as in treatment of childhood obesity. Additional well-designed randomized controlled trials and systematic reviews are need to confirm the conclusion.

• 한국식품영양학회지
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• 제30권2호
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• pp.345-355
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• 2017

The purpose of this study is to provide basic data for the development direction and strategy for medicines through analysis of differences in choice attributes according to customers awareness of medicines and experience. In order to achieve the purpose of this study, a questionnaire survey was conducted on dietary education among national network trainees and lecturers by the Ministry of Food, Agriculture, Forestry and Livestock in Daegu, Gyeongsan, and Ulsan, Seoul from December 5, 2016, respectively. First, factor analysis results showed that six factors were extracted with respect to the choice of the restaurant. Second, the difference in each factor according to the recognition and experience of medicinal herbs was the health related factor as sex, females, age 50~59 years, companion others, and publicity and advertising' factors were more important for publicity and advertisement when the gender was female, the customer's age was 40~49 years, the companion was other, and the time of use was during the weekend. Among the factors related to 'getting dressed', the more important gender was female, age 40~49 years, and the other partner, The factors related to 'time' were as follows: sex, age, age 20~29 years, no accompanying companions, no time of use, and time importance. The 'menu' factors were more important for men, gender, women, age 40~49 years, companion for guitar, and usage time for weekend dinner. The 'service' factors were more important in 'service' as sex, male age, age 20~29, companion family, usage time, and weekend dinner.

• 대한한의학방제학회지
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• 제11권2호
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• pp.19-28
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• 2003

Sokmyeungtang was the representative prescription for Apoplexy under the rule of Tang and Song dynasty of which the cultures were thriving in the history of China. However, the clinical use of Sokmyeungtang has been gradually reduced since Geumwon dynasty of China because it was misunderstood that the dryness heat drugs of pungent in flavor and warm in property such as Ephedra, Pubescent Angelica Root, Chinese Cassia Tree-Bark, Divaricate Saposhnikovia Root, Prepared Aconite Root, Fresh Ginger, and Wildginger Herb included in the presciption for Apoplexy supplemented heat as damaging Yin flood. In fact, the drugs pungent in flavor and warm in property activate exterior and interior circulation, circulate channels and collaterals, promote blood circulation, and remove blood stasis with the side effect of relieving exterior syndrome with drugs warming channels. When treating Apoplexy with Sokmyeungtang, the cold drugs such as Gypsum, Baikai Skullcap Root, and Pueraria Root are prescribed to suppress fire of pungent dryness and to control excessive heat of people with Apoplexy as reducing the effects of hot drugs causing impairment of Yin. For treatment of Apoplexy, the above drugs accelerate blood and Qi circulation in channels and collaterals and then in necrotic tissue of human body as removing blood stasis. Consequently, these drugs improve disorders of capillary tube circulation. If Sokmyeungtang, an old prescription, is properly understood, it will be substantially helpful to all kinds of treatments in clinical cases

• 대한한의학방제학회지
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• 제25권4호
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• pp.537-551
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• 2017

Objectives : Jinnoe-san (JNS) is a novel herbal formula consisting of five oriental medicinal herbs including Polygalae Radix, Prunellae Spica, Perillae Herba, Betulae Cortex, and Lonicerae Flos. In this study, we investigated the effects and molecular mechanism of JNS on Parkinson's disease in vitro model. Methods : The effects of JNS on 1-methyl-4-phenylpyridinium ($MPP^+$)-induced cell death in SH-SY5Y cells were evaluated with a cell viability assay, flow cytometry, and western blots analysis. The effects of JNS on lipopolysaccharide (LPS)-stimulated BV2 microglia were determined with a nitric oxide (NO) assay, enzyme linked immunosorbent assays, and western blots analysis. Result : $MPP^+$-induced cell death in SH-SY5Y cells was significantly reduced by JNS pre-treatment in a dose-dependent manner. JNS inhibited the production of reactive oxygen species, mitochondria dysfunction, and apoptosis induced by $MPP^+$ in SH-SY5Y cells. Furthermore, JNS significantly activated Akt and ERK in SH-SY5Y cells and the ability of JNS to prevent mitochondria dysfunction by $MPP^+$ was antagonized by pre-treatment of LY294002 and PD98059, an Akt and ERK inhibitor, respectively. In addition, JNS inhibited LPS-induced NO and $PGE_2$ production as well as iNOS expression and secretion of TNF-${\alpha}$, pro-inflammatory cytokines without affecting the cell viability. JNS also suppressed LPS-induced ERK activation. Conclusions : These results demonstrate that JNS has a protective effect on the dopaminergic neurons against $MPP^+$-induced neurotoxicity and anti-inflammatory effect on the LPS-stimulated microglia. These findings provide evidences for JNS to be considered as a new prescription for treating Parkinson's disease.

• 대한한의학방제학회지
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• 제25권4호
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• pp.471-481
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• 2017

Objectives : Noemyeong-san (NMS) is a novel herbal prescription composed of five oriental medicinal herbs including Prunellae Spica, Betulae Cortex, Foeniculi Fructus, Asiasari Radix, and Clematidis Radix for treating Alzheimer's disease. In the present study, we investigated the effects and molecular mechanisms of NMS on BV2 microglia to evaluate the potential action of this formula for preventing or treating neurodegenerative disease such as Alzheimer's disease. Methods : To determine the cytotoxicity of NMS on BV2 microglia, the MTT assay was performed. The effects of NMS on lipopolysaccharide (LPS)-stimulated BV2 microglia were determined with a nitric oxide (NO) assay and western blots for inflammatory mediator-related proteins, mitogen activated protein kinases (MAPKs), nuclear factor kappa B (NF-${\kappa}B$) pathway-related proteins, and heme oxygenase-1 (HO-1). Result : NMS inhibited induction of iNOS and COX-2 as well as NO production without affecting the cell viability in LPS-stimulated BV2 microglia. NMS also suppressed activation of ERK and p38 MAPK among main kinases of MAPKs as well as NF-${\kappa}B$ by LPS stimulation. Furthermore, NMS dose-dependently induced the expression of HO-1 and the inhibitory effect of NMS on the production of NO were blocked by pretreatment with an HO-1 inhibitor, Snpp. Conclusions : These results demonstrate that NMS has potent anti-neuroinflammatory effect on the LPS-stimulated microglia. These findings provide evidences for NMS to be considered as a new prescription for preventing or treating neurodegenerative disease such as Alzheimer's disease.

• 대한한방소아과학회지
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• 제26권1호
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• pp.16-35
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• 2012

Objectives The purpose of this study is to investigate various treatments in Japan for atopic dermatitis. The treatments on atopic dermatitis from diet modification and kampo medicine were studied for 11 years (from 2001 to 2011). Methods The search database includes PubMed. To narrow the search, the following key search terms were used: 'atopic dermatitis, diet, japan', 'atopic dermatitis, kampo, japan'. The search was limited to the publication date from 2001 to 2011. Results 1. There are 16 studies on atopic dermatitis treatment by using diets - 2 clinical trials and 12 animal experiments. All researches have revealed that diet modification is effective for atopic dermatitis. 2. There are 6 studies on atopic dermatitis treatment by using kampo medicine - 2 clinical trials and 4 animal experiments. All researches have revealed that kampo medicine is effective for atopic dermatitis. 3. There are 4 studies on atopic dermatitis treatment by using herbs - 4 animal experiments. All researches have revealed that herb is effective for atopic dermatitis. Conclusions Most of the animal experiments were using mice, so the studies on safety and effectiveness are needed to be confirmed to human as well. In the future, systematic guidelines and protocols are necessary for clinical trials and development of successful treatments on atopic dermatitis is needed.

• 생약학회지
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• 제12권1호
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• pp.31-43
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• 1981

In order to determine the effect of Oryeongsan reputed to have diuretic action since Han Dynasty and possible synergetic action of Dianthi Semen, Polygonum avicularis Herba, Kochiae Fructus and Akebiae Lignum, herbs with similar reputation, when added to the above prescription, their decoction powders were solved into distilled water and injected into rabbits through the ear vein. Upon the treatment, the excretion of sodium, potassium and chloride ion together with urine volume was kinetically determined. At the same time the clearance of plasma creatinine and sodium ion was determined and the following results were obtained. Every experimental group demonstrated diuretic action, though feable, of relatively long duration. The diuretic mechanism in the case of Oryeongsan made up by mixing the seperate extracts of individual components and Oryeongsan(A) plus Dianthi Semen was found to be inhibitory effect of renal tubular reabsorption in contrast to the case of Oryeongsan(A) plus Polygonum avicularis Herba, Kochiae Fructus or Akebiae Lignum in which case the diuretic mechanism was found to be glomerular vascular dilatation. The urinary excretion of potassium ion was increased in the case of Oryeongsan(A) plus Dianthi Semen, Kochiae Fructus of Akebiae Lignum whereas in the other cases no similar change was registered. The diuretic action was most remarkable in the case of Oryeongsan(A) plus Polygonum avicularis Herba followed by Oryeongsan(A) plus Kochiae Fructus, Oryeongsan(A) plus Dianthi Semen, extract mixture of individual component of Oryeongsan, Oryeongsan(A) and Oryeongsan(A) plus Akebiae Lignum decreasing order. The duration of diuretic action was found to be 90 minutes in the case of Oryeongsan and mixture of individual component of Oryeongsan, and 60 minutes in the case of Oryeongsan(A) plus Dianthi Semen, Kochiae Fructus or Akebiae Lignum in contrast to the case of Oryeongsan(A) plus Polygonum avicularis Herba which lasted up to 120 minutes.