• 제목/요약/키워드: organic medium

검색결과 759건 처리시간 0.029초

Calcium Alginate에 포괄된 Yeast Invertase의 고정화 효소에 관한 연구 (I. 효소 생산의 최적 조건) (Calcium Alginate-entrapped Yeast Whole-cell Invertase I Optimum Conditions of Invertase Production)

  • 방병호;이상건;양철영
    • 한국식품영양학회지
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    • 제2권2호
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    • pp.8-13
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    • 1989
  • A strain of Saccharomyces cerevisiae BY-366 was found to produce a strong sucrose-hydrolyzing enzyme Using this strain, the optimal culture conditions for the production of invertase were investigated. The results are as follows : 1. For enzyme production, optimal temperature, initial pH and critical concentrations of sucrose and raffinose were 3$0^{\circ}C$, 5.0 and 3.0%, respectively. 2. Enzyme production was reached maximum by organic nitrogen source, 0.3% yeast extract plus 0.5% bactopeptone. 3. It was appeared the presence of 0.1 M Mn2+ and Fe2+ ion was essential factors, on the other hand, 0.1 M Ag+ and Hg2+ ion almost block in yeast growth and enzyme production. 4. Invertase productivity was reached maximum within 3 days on stationary culture with medium-composed of sucrose 3%, bactopeptone 0.5%, yeast extract 0.3%, KEHPO. 0,1%, MgSO4.7H2O 0.05%.

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톱밥을 이용한 미생물사료에 관한 연구 -(I) 리그닌 및 단백질 함량의 변화- (Microbial Treatment of Saw Dust for Animal Food -(I) Changes of Lignin and Protein Contents-)

  • 이배함
    • 한국균학회지
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    • 제3권2호
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    • pp.25-29
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    • 1975
  • 우리나라 담자균류 12균주 중 톱밥의 lignin을 잘 분해하는 균주 Pleurotus ostreatus 1주를 선발할 수 있었다. 이 균주는 톱밥에 20%의 미강을 첨가하고 보통배지에 첨가되는 무기염을 첨가하면 $30^{\circ}C$에서 30일이면 lignin의 49%를 분해할 수 있었고 다시 무기 N을 단백질로 전환시킬 수도 있으며 여기서 단백질 증가율은 26%였다.

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Zoogloea ramigera에 의한 생물고분자 생산에 관한 연구 (The Production of Biopolymer by Zoogloea ramigera)

  • 안대희;권해수정윤철
    • KSBB Journal
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    • 제7권3호
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    • pp.166-171
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    • 1992
  • 생물응집체 개발을 목적으로 Zoogloea ramigera115를 이용하여 균주배양 및 생물고분다 생성실험을 시시하였다. 다당류 최고 생성률을 얻을 수 있는 최적기질로 탄소원으로는 glucose, 질소원으로는 $NaNO_3$ 유기영양원으로는 yeast extract가 선택되었다. 초기 pH가 6일 때 가장 높은 세포성장과 점성을 나타내었다. 생성돈 생물고분자는 ultrasonication과 고속 원심분리를 거친 후 propanol 첨가에 의해 효율적으로 추출되었다. 생물고분자는 jae test에 의한 응집실험에서 높은 침강성을 보여 응집제 사용가능성을 확인하였다.

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물/유기용매 불혼합 이성분계에서의 반응염색(Ⅱ) - C. I. Reactive Blue 203을 이용한 견섬유의 염색 - (Reactive Dyeing in Immiscible Two-phase System of Water/Organic Solvent(Ⅱ) - The Dyeing of Silk with C. I. Reactive Blue 203 -)

  • 김태경;김미경;임용진;조광호
    • 한국염색가공학회지
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    • 제13권2호
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    • pp.42-42
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    • 2001
  • Following the prior studs regarding that 1.0g of cotton fabric cound be dyed uniformly with a reactive dye in the solvent mixture of 2㎖ of water and 23㎖ of dichloromethane, silk fabric was dyed with C. I. Reactive Blue 203 in the water/dichloromethane two-phase immiscible solvent media. In order to minimize dye loss due to its hydrolysis, the reactive dyeing was carried out in dichloromethane containing a small amount of water. With only 3㎖ of water in 22㎖ of dichloromethane, 1.0g of silk fabric could be dyed perfectly. The uptake ratio was increased greatly, compared with that of normal reactive dyeing in a water medium. It would seem that the one of hydrophobic solvents, dichloromethane, can assist the even dyeing as it disperses a small amount of dye-dissolved water phase and conveys this water phase to the fabric entirely and uniformly.

물/유기용매 불혼합 이성분계에서의 반응염색(I) (Reactive Dyeing in Immixcible Two-phase System of Water/organic Solvent (I))

  • 김태경;윤석한;임용진;조광호
    • 한국염색가공학회지
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    • 제13권1호
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    • pp.55-60
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    • 2001
  • Cotton fabric was deed with a reactive dye in water/dichloromethane two-phase immiscible solvent media. In order to minimize dye loss due to its hydrolysis, the reactive dyeing was carried out in dichloromethane containing a small amount of water. With only 2ml of water in 23m1 of dichloromethane, 1.0g of cotton fabric could be dyed perfectly. The uptake ratio was increased greatly, compared with that of normal reactive dyeing in a waters medium. It would seem that the one of hydrophobic solvents, dichloromethane, can assist the eden dyeing as it disperses a small amount of dye-dissolved water phase and conveys this water phase to the fabric entirely and uniformly.

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물/유기용매 불혼합 이성분계에서의 반응염색(II) - C. I. Reactive Blue 203을 이용한 견섬유의 염색 - (Reactive Dyeing in Immiscible Two-phase System of Water/Organic Solvent (II) - The Dyeing of Silk with C. I. Reactive Blue 203 -)

  • 김태경;김미경;임용진;조광호
    • 한국염색가공학회지
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    • 제13권2호
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    • pp.128-134
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    • 2001
  • Following the prior studs regarding that 1.0g of cotton fabric round be dyed uniformly with a reactive dye in the solvent mixture of $2m\ell$ of water and $23m\ell$ of dichloromethane, silk fabric was dyed with C. I. Reactive Blue 203 in the water/dichloromethane two-phase immiscible solvent media. In order to minimize dye loss due to its hydrolysis, the reactive dyeing was carried out in dichloromethane containing a small amount of water. With only $3m\ell$ of water in $22m\ell$ of dichloromethane, 1.0g of silk fabric could be dyed perfectly. The uptake ratio was increased greatly, compared wish that of normal reactive dyeing in a water medium. It would seem that the one of hydrophobic solvents, dichloromethane, can assist the Even dyeing as it disperses a small amount of dye-dissolved phase and conveys this water phase to the fabric entirely and uniformly.

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세균 Pseudomonas sp. YK-32 균주에 의한 Formaldehyde 분해 최적조건 (Optimum Conditions of Formaldehyde Degradation by the Bacterium Pseudomonas sp. YK-32)

  • 김영목;이윤경;김경란;이은우;이명숙
    • 한국수산과학회지
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    • 제41권2호
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    • pp.102-106
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    • 2008
  • Formaldehyde, an indoor volatile organic compound, is considered toxic due to its carcinogenic risk. Recently, we isolated a formaldehyde-degrading bacterium Pseudomonas sp. YK-32. A crude enzyme prepared from YK-32 also degraded formaldehyde, suggesting that YK-32 cells have formaldehyde hydrogenase activity which is one of the important factors in formaldehyde degradation. The formaldehyde hydrogenase activity was increased 1.25 fold by adding 0.1 % glucose and formaldehyde to the culture medium. In addition, treatment with 1 mM EDTA as a permeabilizer promoted the degradation of formaldehyde and increased the enzymatic activity.

Electrochemical Investigation of Inhibitory of New Synthesized 3-(4-Iodophenyl)-2-Imino-2,3-Dihydrobenzo[d]Oxazol-5-yl 4-Methylbenzenesulfonate on Corrosion of Stainless Steel in Acidic Medium

  • Ehsani, Ali;Moshrefi, Reza;Ahmadi, Maliheh
    • Journal of Electrochemical Science and Technology
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    • 제6권1호
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    • pp.7-15
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    • 2015
  • 3-(4-Iodophenyl)-2-imino-2,3-dihydrobenzo[d]oxazol-5-yl 4-methylbenzenesulfonate (4-IPhOXTs) was synthesized and its inhibiting action on the corrosion of stainless steel 316L (SS) in sulfuric acid was investigated by means of potentiodynamic polarization and electrochemical impedance spectroscopy (EIS). The results of the investigation show that this compound has excellent inhibiting properties for SS corrosion in sulfuric acid. Inhibition efficiency increases with increase in the concentration of the inhibitor. The adsorption of 4-IPhOXTs onto the SS surface followed the Langmuir adsorption model with the free energy of adsorption ΔG0ads of −8.45 kJ mol−1 . Quantum chemical calculations were employed to give further insight into the mechanism of inhibition action of 4-IPhOXTs.

두 종모드 레이저 빛 사이의 맥놀이 신호를 이용한 간섭계에 의한 유효 광학 두께 측정 (Measurement of the effective optical thickness of optical media using intermode beat interferometer scheme)

  • 윤신영;조규만;이용산
    • 한국광학회지
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    • 제8권1호
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    • pp.26-30
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    • 1997
  • 서로 수직으로 편광되고 두개의 종모우드로 발진하는 주파수가 안정화된 He-Ne 레이저를 이용하여 넓은 측정 범위를 갖는 헤테로다인 간섭계를 구성하였다. 두 모우드 사이의 맥놀이 주파수인 684MHz에 대한 라디오 주파수 간섭을 이용하여 약 .+-.1.74.mu.m의 분해능으로 광학매질의 광학두께를 측정할 수 있는 장치를 구성하였다. 이와 같은 측정장치를 이용하여 Nd:YAG 레이저의 수동형 Q-스위칭에 포화흡수체로 사용되고 있는 유기염료 박막의 두께와 국부적인 광학두께의 변화에 대한 map을 구할 수 있었다.

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Selected Strains of Ginseng with Ge-organic Compounds and ik Effects.

  • Larisa, Slepyan
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 1998년도 Advances in Ginseng Research - Proceedings of the 7th International Symposium on Ginseng -
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    • pp.71-82
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    • 1998
  • The first original inducer of interferon had been synthesized in Russia in 1965-1967. It was 2-car-boxiethyl-germanysesquioxan (Lx-13) and other-1 hydroxigermanytrans-monohydral (Lx-5), These compounds have very high induct$\circledcirc$on of interferon. The strain of Panax ginseng C. A. Meyer was recultivated on medium with different concentiation of (Lx-13) and (Lx-5) many times. The strain was stabilized when biomass of cells contain of the Ge form 10${\times}$10-3mg% to 2.3x10-3mg% to ashes, Biomass are hard physical work (swimming test) and also had stress-protective, immunomodulating action and anticarcinogenic effect hampered the sarcoma-180 by 33-80% and suppressed quantity of metanes on 43-46%.

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