• Journal of Pharmaceutical Investigation
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• v.35 no.6
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• pp.423-429
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• 2005

A selective and sensitive reversed-phase HPLC method for the determination of fenoprofen in human serum was developed, validated, and applied to the pharmacokinetic study of fenoprofen calcium. Fenoprofen and internal standard, ketoprofen, were extracted from human serum by liquid-liquid extraction with diethyl ether and analyzed on a Luna C18(2) column with the mobile phase of acetonitrile-3 mM potassium dihydrogen phosphate (32:68, v/v, adjusted to pH 6.6 with phosphoric acid). Detection wavelength of 272 nm and flow rate of 0.25 mL/min were fixed for the study. The assay robustness for the changes of mobile phase pH, organic solvent content, and flow rate was confirmed by $3^{3}$ factorial design using a fixed fenoprofen concentration $(2\;{\mu}g/mL)$ with respect to its peak area and retention time. And also, the ruggedness of this method was investigated at three different laboratories using same quality control (QC) samples. This method showed linear response over the concentration range of $0.05-100\;{\mu}g/mL$ with correlation coefficients greater than 0.999. The lower limit of quantification using 1 mL of serum was $0.05\;{\mu}g/mL$, which was sensitive enough for pharmacokinetic studies. The overall accuracy of the quality control samples ranged from 92.27 to 109.20% for fenoprofen with overall precision (% C.V.) being 5.51-11.71 %. The relative mean recovery of fenoprofen for human serum was 81.7%. Stability (freeze-thaw, short and long-term) studies showed that fenoprofen was not stable during storage. But, extracted serum sample and stock solution were allowed to stand at ambient temperature for 12 hr prior to injection without affecting the quantification. The peak area and retention time of fenoprofen were not significantly affected by the changes of mobile phase pH, organic solvent content, and flow rate under the conditions studied. This method showed good ruggedness (within 15% C.V.) and was successfully used for the analysis of fenoprofen in human serum samples for the pharmacokinetic studies of orally administered Fenopron tablet (600 mg as fenoprofen) at three different laboratories, demonstrating the suitability of the method.

• Applied Biological Chemistry
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• v.36 no.4
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• pp.277-283
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• 1993

In order to brew foxtail millet wine, a folk wine of Cheju, properties of raw materials, optimum brewing conditions were inveatigated. Carbohydrate and crude fat content of glutinuous foxtail millet are 71.27% and 3.47%, respectively. Since the ratio of water to steamed millet and ethanol concentration of wine showed negative correlation, less than 250% water had to added to steamed millet to maintain ethanol concentration in wine above 13%, Sugar consumption and ethanol production increased rapidly for the first 2 days, and main fermentation was done in 4 days. Ethanol concentrations were $13.0{\sim}13.4%$ when foxtail millet was used, and they were $14.0{\sim}14.3%$ for the mixture substrates of 90% millet and 10% rice or barley. Organic acids in millet wine were lactic acid, malic acid and succinic acid. The residual carbohydrates after fermentation were mainly xylose and oligosaccharides. A trace of methanol was detected in millet wine. The content of fusel oil was low, while the concnetration of organic acids was high. Optimum conditions for millet wine-making were as follows. Glutinuous foxtail millet with 10% rice as fermentation source need to be soaked in water and steamed for enough time. Water was added to steamed millet with the ratio of 2 : 1. The resulting mixture was stmnultaneously saccharified and fermented by Aspergillus orzae and Saccharomyces cerevisiae IAM 4274 at $20^{\circ}C$ for a week. Millet wine was prepared after filtering fermented broth while pressing for a week.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.2
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• pp.150-158
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• 1993

In order to investigate the content and seasonal variation of the extractive components including taste compounds, free amino acids, nucleotides and related compounds, quanternary ammonium bases, and guanidino compounds of ascidian collected from the south coast of Korea were determined bimonthy from April to October in 1990. The extractive nitrogen was composed of $60{\sim}62\%$ as free amino acids, $12{\sim}16\%$ as betaines, $5{\sim}9\%$ as nucleotides, and others as trimethylamine oxide(TMAO) and total creatinine. The muscle of ascidian was rich in such free amino acids as taurine, proline, glutamic acid, glycine and glycinebetaine. Most of nitrogenous compounds in the extractives showed a marked seasonal variation with a maximum in summer or autumn. AMP content was relatively high among nucleotides. Succinic acid, malic acid, lactic acid and pyroglutaric acid were the major organic acids in ascidian. The results of omission test suggested that the taste of ascidian is attributed to mainly free amino acid, betaines, nucleotides and nonvolatile organic acid in order.

• Korean Journal of Soil Science and Fertilizer
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• v.6 no.1
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• pp.9-16
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• 1973

In order to observe the effect of a continuous application of herbicides on the chemical properties of upland soil, samples were collected and analysed from a field experiment continuously applied with herbicides and from two pot experiments, one with a heavy dose and the other with a normal dose of herbicides, applied continuously. The soil samples were collected after three harvests of radish. The results of these analysis were summarized as follows; 1. Of herbicides, M.O., Lorox, 2, 4-D, Lasso, and P.C.P., the Lorox decreased soil pH and organic carbon content in soil and increased exchange and hydrolytic acids, and exchangeable potassium. The herbicide also showed an indication to decrease the exchangeable calcium, magnessium and sodium in soil. It seemed that such changes are mainly due to the indirect effect of Lorox or its metabolites, such as stimulating soil microorganisms, rather than direct chemical nature of them. 2. The other herbicides investigated in this study seemed to increase the organic carbon content in soil. 3. In addition, the continuous application of herbicides may result in an alteration of weed species in the field. All such facts might affect the properties of soil, which may call for a further detailed investigation having a set up of a long term experiment.

• Food Science and Preservation
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• v.17 no.2
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• pp.169-173
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• 2010

Although fruit grown under sustainable farming conditions is believed to be healthier for humans than is fruit grown by conventional cultivation, little scientific information on the characteristics of fruit produced using these two farming systems is available in Korea. Therefore, weinvestigated fruit quality, total polyphenolic contents, and anti-oxidant activities in 'Niitaka' pears grown under sustainable and conventional farming management systems. Treatmentsincluded use of a chitin compost admixed with liquid chitin fertilizer (plot A), and use of a chitin compost admixed with liquid chitin fertilizer treated by infrared radiation (plot B). Plots C and D used conventional management systems. Fruit qualities at harvest differed between both sustainable plots A and B and the conventional plots C and D. The average values of firmness and total polyphenolic content in fruit harvested from sustainable plots were not significantly greaterthan those of fruit grownin conventional plots, after 60 days of storage. Fruit grown in all plots had low polyphenol oxidase (PPO) activity at harvest but this increased during storage. Fruit from sustainable plot B showed an increased electron donating ability compared with fruit grown using the other systems.

• Journal of the Korea Organic Resources Recycling Association
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• v.3 no.2
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• pp.69-78
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• 1995

When the food garbage of general hospital was dried by the rapid steam drying process, the water content was changed to 1.3% from 77.8~82.8%. In this experiment, weight reduction rate was 80%, and electricity consumption was 2.4Kwh. Dried compost from this rapid steam drying process was brown pellets, which was consist of 27.77% crude protein and 3.19% crude fiber. Even though these pellets were slightly short of crude fat and crude ash content, these were analysed as a possible supplementary feed for pig. On the condition of drying food garbage mixed with 5% pulverized chaff, the necessary drying time was shortened by 1 hour, weight reduction rate was 76%, and reduction rate of electricity consumption was 42%. But contents of crude fiber and crude ash were increased to about 2 times. In case of adding new food garbage continuously to the composted food garbage mixed with 3.4% pulverized chaff, weight reduction rate and contents of crude fiber and crude ash were decreased gradually, but contents of crude protein and crude fat were increased. In case of composting food garbage from buffet, both drying time and electricity consumption were reduced, and ingredients of compost were higher than that of assorted feed for pig in the market.

• Korean Journal of Agricultural Science
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• v.5 no.2
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• pp.115-119
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• 1978

To improve the feed value of rice straw, Tongil type rice straw and Persicaria Thunbergii Gross var. coreana Gross ex Nakai were mixed in the ratios of 10 : 90 (T1), 20 : 80 (T2), 30 : 70 (T3) and 40 : 60 (T4) in fresh weight and ensiled for 6 weeks. The chemical composition of fresh materials and silage, the organic acid contents and pH in silage were determined. And the results obtained were summarized as follow: 1. The crude protein content in rice straw was 8.18% of DM basis and that in Persicaria Thunbergii was 14.47%. And the nitrogen-free extract content in rice straw was 44.66% and.. that in Persicaria Thunbergii was 51.13%. 2. The optimum moisture contents were appeared in T2 as 72.12% and T3 as 66.43%. Considerably high crude protein contents were determined from all of treatments. 3. The pH of silage was varied from pH 3.8 of T1 to pH 4.5 of T2. And Total organic acid contents were increased from T1 to T4, but the percentage of lactic acid contents to total acid contents were decreased. 4. It was concluded that good quality silage could be made from rice straw with Persicaria Thunbergii and the best ratios of rice straw: Persicaria Thunbergii were between 20 : 80 and 30 : 70 in fresh weight.

• Journal of Radiation Protection and Research
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• v.27 no.2
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• pp.101-110
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• 2002

The concentrations and activity ratios of $^{137}Cs,\;^{90}Sr\;^{238}Pu$ and $^{239-240}Pu$ in sediment($0sim}20\;$cm) at 15 coastal stations of Korea were determined. The mean concentrations of $^{137}Cs,\;^{90}Sr\;^{238}Pu$ and $^{239-240}Pu$ are $2.24{\pm0.79\;Bq{\cdot}kg^{-1}-dry,\;0.20{\pm}0.04 Bq{\cdot}kg^{-1}-dry,\;0.009{\pm}0.005\;Bq{\cdot}kg^{-1}-dry}$ and $0.27{\pm}0.17\;Bq{\cdot}kg^{-1}-dry$, respectively. The mean activity ratios of $^{137}Cs,\;^{90}Sr$, $^{239-240}Pu/^{137}Cs$ and $^{238}Pu/^{229-240}Pu$ and atomic ratio of $^{240}Pu/^{239}Pu$ are $11.2{\pm}2.9,\;0.123{\pm}0.053$ and $0.033{\pm}0.017$ and $0.218{\pm}0.036$, respectively. The concentrations and activity ratios in sediment samples are similar to those reported from neighbouring country in the northern hemisphere. The correlation coefficient of $^{137}Cs$ and $^{239-240}Pu$ is 0.80. The correlation coefficient of $^{137}Cs$ and soil organic matter(SOM), and $^{239-240}Pu$ and clay content are 0.69 and 0.67, respectively.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.8
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• pp.1037-1043
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• 2008

In this experiment, citrus juice was fermented by Gluconacetobacter hansenii TF-2, an isolate from tea fungus to develop a new type of acidic beverage. The juice broth is made by fermenting of $11{\sim}17%$ (v/v) juice and sweetened with sucrose (initial sucrose $10^{\circ}Brix$). The fermentation by G. hansenii TF-2 was initiated by adding 5% (w/v) of seed gel (pellicle, tea fungus) which was previously cultured in the same medium (fresh juice broth) and the fermentation was carried out in a dark incubator at $28{\sim}30^{\circ}C$ for about 15 days. During the fermentation a pellicle grew on the surface of the fermenting fluid and acids were produced. Fermented fluid (beverage) was centrifuged at 7,000 rpm for 15 min for further analyses. The highest amount of the other metabolites including organic acids were observed in 5 to 10 days. Major acids were acetic acid (fermented citrus beverage, CB). After 15 days of fermentation, organic acid content such as acetic acid in fermented beverage was measured to be $183.5{\sim}186.6\;ppm$. Free sugars content in CB were 56.8%, 35.1%, 40.7% and 63.2% of unfermented sucrose, glucose, fructose and sorbitol, respectively. When the growth rate of inhibitory effect of the fermented beverage was measured by using several species of food-related bacteria, the beverage fermented with CB exhibited the strongest inhibition against gram-negative (E. coli and Sal. Typhimurium). Its inhibition rate was between $71.9{\sim}94.0%$ at CB. Fermented beverage has shown effectiveness for antimicrobial activity against some species of food-related bacteria.

• Journal of the Korean earth science society
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• v.18 no.6
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• pp.540-552
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• 1997

For the scientific and systematic study of sediment deposits along Yeongsan river, total 13 core samples were taken from Yeongsan river. The thickness of sediment deposits is 22.0m at YS 1, 20.0m YS 2, 18.5m YS 3, 12.0m YS 4, 3.0m YS 5, 5.5m YS 7, 3.0m YS 8, 5.9m YS 9, 5.0m YS 10 on main stream of Yeongsan river. The thickness of deposit is increasing from upstream to downstream. The composition of sediments dominates sandy gravel on the upstream and the lower part on the downstream, and mud deposits of $11m{\sim}16m$ thick on the upper part of on the downstream(YS 1, YS 2, and YS 3). Contents of organic carbon and calcium carbonates occur being contrary to each other toward upstream. The content of organic carbon increases upstream, that of calcium carbonates decreases. According to size analysis of sediment, the content of gravel increases and mud decreases from downstream to upstream. Sediment deposits can be divided into two types whether gravel is or not. But this boundary is clear in the downstream(YS 1$\sim$YS 4) but is vague in the upstream. The result of this study could be provided engineering, environment, and architecture with geological background as the essential basis.