• 제목/요약/키워드: optimum extraction condition

검색결과 230건 처리시간 0.025초

Amylase를 이용한 인삼엑기스의 제조 (The Studies on the Production of Ginseng Extract by Amylase)

  • 임무현;조규성;김해중;주현규
    • Journal of Ginseng Research
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    • 제3권2호
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    • pp.134-143
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    • 1979
  • It order to extract the soluble organic substances of Korean ginseng effectively, the ginseng extract have been made by using amylase. The investigation on the optimum condition of enzyme reaction was carried out, and the amounts of gained extract and its saponin pattern were compared among the ethanol extract, water extract and enzyme extract. The results obtained are summerized as follows. 1. The gaining ratio or ginseng extract was the highest value when the raw ginseng and dried ginseng were extracted in the concentration of 7.5% and 5% with 0.3%∼0.6% enzyme for 25 hour. 2. The amounts of ethanol extract, water extract and enzyme extract were 9.14%, 17.23% and 23.73% in case of raw ginseng and 64.09%, 72.52% and 74.36% in case of dried ginseng, respectively. The amount of enzyme extract was increased as much as 6∼14% in case of raw ginseng, and 2∼10% in case of dried ginseng compared with that of ethanol and water extract. 3. The absolute content of saponin was nearly constant in spite of the different extraction method and all of the ginseng saponin pattern of thin-layer chromatograms were almost same.

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오배자의 염색성에 관한 연구 (A study on the mordanting and dyeing properties of Rhusjara ica Dye)

  • 주영주
    • 한국의류학회지
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    • 제22권8호
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    • pp.971-977
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    • 1998
  • For the purpose of standardization and practicability of nautral dyeing, the mordanting and dyeing properties of Rhusjara ica was studied. Appropriate extraction, dyeing and mordanting condition of Rhusjara ica were determined, and the effect of mordanting method on dye uptake and color fastness of dyed fabric was investigated. The most absorbance of Rhusjara ica solution was 299 nm. The color of Rhusjara ica solution was affected by pH 8~9. The optimum temperature to extract Rhusjara ica was 6$0^{\circ}C$ and dyeing solution for 1 hour. Effective dyeing time to silk was 60min. Effective mordanting temperature was 80~10$0^{\circ}C$, and its time was 30 min. K/S value of dyeing fabrics was recoginazed by mordanting treatment, specially Fe, Al, Cu. K/S value of pre-mordanting was higher than post-mordanting. In the case of Rhusjara ica fastness was increased by mordanting treatment.

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괴화의 염색성에 관한 연구 (The Study on the Mordanting and Dyeing Properties of Sophora Japonica L.)

  • 주영주;소황옥
    • 복식
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    • 제52권3호
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    • pp.19-27
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    • 2002
  • This paper surveys the mordanting and dyeing properties of Sophora japonica L. Appropriate extraction, dyeing and mordanting condition of Sophora japonica L. were determined, and the effect of mordanting method on dye uptake and color fastness of dyed fabric was investigated. The maximum absorbance of Sophora japonica L. solution was 367.6mn, rutin solution was 365.6mn. The color of Sophora japonica L. solution was affected at pH 2 and pH 8~9. The optimum temperature to extract Sophora japonica L. was during 1 hour in 8$0^{\circ}C$. The effective dyeing temperature and time of silk were 10$0^{\circ}C$, 60min. K/S value of dyeing fabrics was increased by pre-mordanting treatment, especially Fe, Sn, Cr. In the case of Sophora japonica L. light fastness was increased by Fe mordanting. Perspiration fastness was better in acidic solution than that in alkaline solution. Fastness to rubbing and dry-cleaning were good in general.

방선균 J-144K가 생산하는 Adenosine Deaminase Inhibitor에 관한 연구 (Studies on the Adenosine Deaminase Inhibitor Producing Actinomycetes J144-K)

  • 전홍기;김상웅;조영배;이인
    • 생명과학회지
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    • 제6권2호
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    • pp.120-128
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    • 1996
  • In the screening of actinomycetes culture filtrate for inhibitor of adenosine deaminase, a novel inhibitor was found in a cultured broth of strain J-144K. The optimum conditions for the adenosine deaminase inhibitor production from the isolated strain J-144K were evaluated. This strain showed the maximum yield of adenosine deaminase inhibitor when grown at pH 7.0 and 30$\circ$C for 60 hours in the medium of 1.0% dextrose, 0.5% yeast extract, 0.5% peptone and 0.1% KH$_{2}$PO$_{4}$ under the aerobic condition. Through the activated charcoal extraction, methanol fractionation, Dowex 50 H$^{+}$ X-8 ion exchange column chromatography, Dowex CI$^{-}$ X-8 ion exchange column chromatography, and Sephadex G-15 gel filtration procedures, this inhibitor was purified with three materials.

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MC-50 싸이클로트론의 설계 특징과 동작 특성 (Design Features and Operating Characteristics of the MC-50 Cyclotron)

  • Bak, Hae-Ill;Bak, Joo-Shik
    • Nuclear Engineering and Technology
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    • 제21권3호
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    • pp.216-222
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    • 1989
  • 원자력병원에 설치되어 있는 MC-50 싸이클로트론은 중성자 치료와 의학용 동위원소 생산을 위해 가동중에 있다. 본 논문에서는 MC-50의 설계 특징, 기계적 구조, 가동 특성에 대하여 기술하고 있다. 본 싸이클로트론의 최적 가동 조건은 반복적인 운전에 의해 결정되었으며, 내부 빔의 성능은 싸이클로트론 반경의 함수로서 내부 빔의 세기 및 공간 분포 측정을 통해 조사되어졌다. 일상적으로, 인출효율이 61%일 때 40$\mu$A세기의 50 MeV 양성자 빔을 얻었다.

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Streptomyces aburabiensis SMF30이 생산하는 Cathepsin B 저해물질의 발효생산 및 특성분석 (Production and Physico-chemical Properties of Cathepsin B Inhibitor from Streptomyces aburabiensis SMF 30)

  • 최영출;김인섭;박상진;윤성준;이계준
    • 약학회지
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    • 제39권3호
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    • pp.306-313
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    • 1995
  • The aim of the present study was to produce low molecular weight cathepsin B inhibitor. A strain of Streptomyces aburabiensis isolated from soil in Korea was selected and the optimum condition for the production of the inhibitor was evaluated. Glucose and soytone were selected as best carbon and nitrogen sources, respectively. From the kinetic analysis in batch fermentation, it was found that the specific cathepsin B inhibitor production rate (q$_{p}$) was linearly related to specific growh rate ($\mu$). The inhibitor in culture filtrate was purified by adsorption on activated charcoal, butanot extraction, silica gel chromatography, ion exchange chromatography using Dowex-1 (Cl form) and Amberlite IRC-50 (H$^{+}$ form), and preparative TLC. From the UV, IR, Mass spectroscopy and $^{1}$H-NMR, the inhibitor was thought to be a new inhibitor of which molecular weight was 199.

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택사(澤瀉)의 재배조건(栽培條件)이 수량(收量) 및 유효성분(有效成分)의 함량(含量)에 미치는 영향(影響) (Effects of Cultural Condition on the Yield and Contents of Effective Components in Alisma orientale(Samuels.) Juzepcz.)

  • 김금숙;김정곤;성재덕;박창기;서형수;곽용호
    • 한국약용작물학회지
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    • 제4권2호
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    • pp.139-144
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    • 1996
  • 택사(澤瀉)의 품질평가(品質評價) 기준의 하나로서 혈중콜레스테롤 강하작용(降下作用)을 하는 Alisol B-monoacetate의 적절한 추출조건(抽出條件)과 재배방법상(栽培方法相)의 정식깊이를 $0{\sim}1$, $2{\sim}4$, $5{\sim}7$, $8{\sim}10cm$ 등(等) 4수준(水準)으로 달리할 때의 수량(收量) 및 품질(品質)의 변이(變移)를 구명하고 적절한 수확시기(收穫時期) 및 구경(球莖) 크기별 일반성분(一般成分) 및 유효성분(有效成分) 함량변이(含量變移)를 구명 하였던 바 결과를 요약(要約)하면 다음과 같다. 1. Alisol B-monoacetate의 HPLC 분석(分析) 조건(條件)으로는 Nova-Pak C18 column를 $35^{\circ}C$ 일정한 온도로 유지해 줌으로써 UV 208nm에서 약(約) 20분대(分代)의 양호(良好)한 HPLC 크로마토그람 분리(分離) peak를 구하였다. 2. Alisol B-monoacetate는 $25^{\circ}C$ 진탕 추출시 0. 402%, $40^{\circ}C$, $60^{\circ}C$ 가온 환류추출시 각각(各各) 0. 425%, 0. 431% 용출(溶出)되었으며 이때 추출회수(抽出回數)는 매회(每回) 30분(分)씩 추출(抽出)하여$25^{\circ}C$ 진탕 추출시는 2회(回), $40^{\circ}C$, $60^{\circ}C$ 가온 환류추출시 는 3회(回) 추출(抽出)이 적합(適合)하였다. 3. Alisol B-monoacetate는 11월(月) 30일(日) 수확한 택사구경(澤瀉球莖)이 10월(月) 30일(日) 수확(收穫)한 것보다 함량(含量)이 0. 25% 즉 1.5배(培) 가량 크게 증가(增加)하였으나 다른 일반성분(一般成分)의 경우 조단백질(組蛋白質) 함량(含量)은 10월(月), 11월(月) 수확시 각각 평균(平均) 24. 8%, 27. 3%였고 개분함량(漑粉含量)은 10월(月), 11월(月) 각각 30. 8%, 27, 7%로서 수확시기별(收穫時期別) 함량(含量)에는 변이폭(變移幅)이 크지 않았다. 이를 종합하면 유효성분(有效成分)의 함량(含量)이 높은 11월(月) 30일(日) 수확(收穫)이 10월(月) 30일(日) 수확(收穫)보다 유리(有利)할 것으로 판단된다. 4. 구경(球莖) 크기별 약효성분(藥效成分)의 함량변이도 커서 10월(月), 30일(日) 경우 S가 0. 53%로 가장 높고 M, L 순(順)으로 구경(球莖)이 커지면서 함량(含量)이 감소(感少)하였다. 5. 정식(定植)깊이 $0{\sim}1cm$가 다른 정식(定植)깊이에 비교하여 수량(收量)이 206kg/10a로 가장 많았으며 유효성분(有效成分) 변이(變移)는 $0. 33{\sim}0. 39%$로서 정식(定植)깊이에 따라 크지않은 것을 고려하면 택사(澤瀉) 재배시(栽培時) 정식(定植)깊이는 $0{\sim}1cm$가 가장 유리(有利)할 것으로 판단된다.

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소형실험태양(小型實驗太陽)연못에서 열저장층(熱貯藏層)의 두께에 관(關)한 실험적(實驗的) 연구(硏究) (Experimental Study on Thicknesss of Heat Storage Zone in Small Solar Pond)

  • 박이동;서지원
    • 태양에너지
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    • 제7권2호
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    • pp.22-29
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    • 1987
  • This paper dealed with thickness variation of bottom heat sotrage zone due to salinity and flow rate of extration hot brine in small test solar pond (0.5m wide, 0.5m high, 1.0m long). Testing apparatus and situation were follows: 7.1 cm of height of suction diffuser and 1.8cm of height of discharge diffuser above the test pond respectively, 0.3cm of slot size of suction diffuser, 1.0cm of slot size of discharge diffuser, 47cm of length of the slot; heating of hot water ($75^{\circ}C$) through separated hot water tank, discharge of the brine into storage zone through discharge diffuser, the extration of the brine through suction diffuser, circulation of the extracted brine through a heat exchanger (cooler). Following results were obtained through the experiments. 1. In small test solar pond, the typical three zone which showed up in real solar pond were established. 2. Richardson Number was used more effectively to confirm hydrodynamic stability of the stratified flow. 3. The thickness of non convective layer had a great effect on the heat storage of the bottom convective layer, then the temperature of bottom convective layer had a relation to that of upper convective layer. 4. Optimum operating condition in the test pond was on 10%-15% of salt concentration and $0.05m^3/hr$ of flow rate of extraction hot brine. 5. Following thickness of 3 zones were available to obtain under optimum operation condition: o bottom storage zone: $30%{\pm}10%$ of total pond depth o non-convective zone: $40%{\pm}10%$ of total pond depth o Upper surface zone: $20%{\pm}10%$ of total pond depth.

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도토리껍질 추출액의 염색성에 관한 연구 (The Study on the Dyeing Properties of Acorn Shell Extract)

  • 주영주
    • 복식
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    • 제56권1호
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    • pp.143-149
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    • 2006
  • For the purpose of standardization and practicability of natural dyeing, the mordanting and dyeing properties of Acorn Shell was studied. Appropriate extraction, dyeing and mordanting condition of Acorn Shell were determined, and the effect of mordanting method on dye uptake and color fastness of dyed fabric was investigated. The maximum absorbance of Acorn Shell solution was at 287.5nm and 294nm. The pH effect was stable in the color difference changes. The optimum temperature to extract Acorn Shell was during 1 hour in $60^{\circ}C$. The effective dyeing temperature and time of silk were $80{\sim}100^{\circ}C$, 1hour. The silk fabrics dyed with Acorn Shell appeared yellow-brown. K/S value of dyeing fabrics was increased by mordant treatment. K/S value of post-mordanting was higher than pre-mordanting. Mordanting treatment attected color change of the dyed silk. In the case of Acorn Shell light fastness of appeared more than 3-5grades by post-mordanting. Abrasion fastness of appeared more than 3-5grades. Perspiration fastness of appered more than $3{\sim}5grades$ by mordant treatment but Cu mordant treatment was $2-5{\sim}4grades$. Dry-cleaning Fastness appered more than 4 grades except Cu mordant treatment. These fastness improvement were generally effective for mordant treatment, specially Al, Sn, Cr.

갈근추출액의 염색성에 관한 연구 (The Study on the Dyeing Properties of Puerariae Radix Extract)

  • 주영주
    • 복식
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    • 제55권8호
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    • pp.154-160
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    • 2005
  • For the purpose of standardization and practicability of natural dyeing, the mordanting and dyeing properties of Puerariae Radix was studied. Appropriate extraction, dyeing and mordanting condition of Puerariae Radix were determined, and the effect of mordanting method on dye uptake and color fastness of dyed fabric was investigated. The maximum absorbance of Puerariae Radix solution was at 290nm and 301nm. The pH effect was stable in the color difference changes. The optimum temperature to extract Puerariae Radix was during 1 hour in loot. The effective dyeing temperature and time of silk were 80$\~$ 100$^{circ}C$, 1hour. The silk fabrics dyed with Puerariae Radix appeared yellow-brown. US value of dyeing fabrics was increased by Fe, and Cu mordant treatment. Mordanting treatment method affected color change of the dyed silk. In the case of Puerariae Radix light fastness of appeared more than 2-5 grades by mordant treatment. Perspiration fastness of appered more than 4 grades by Al, Sn, Cr mordant treatment. Fastness of abrasion and dry-cleaning appered more than 4-5 grades. These fastness improvement were generally effective for mordant treatment, specially Al, Sn, Cr.