• The Journal of the Convergence on Culture Technology
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• v.10 no.6
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• pp.315-319
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• 2024

Magnolia obovata is a naturalized tree that was introduced from Japan for landscaping purposes and is spreading naturally. Its value as a landscape tree is high and its usefulness as a medicine is recognized, but mass propagation methods have not been developed due to the low success rate of cuttings. In this study, field cuttings were attempted using branches pruned in the fall as cuttings. The cuttings were cut to 30 cm in length and planted so that more than half were buried in the ground. A sunshade was put up with black vinyl, and a double vinyl tunnel was made with transparent vinyl to protect the cuttings. The double vinyl tunnel was removed at the end of March. Watering of cuttings mainly depended on natural rainfall, and cuttings were judged successful when new stems were 10 cm or longer along with leaves grown from cuttings in early June and early August. All of the thin cuttings with a diameter less than 8 mm failed to produce leaves, 4 out of 30 cuttings with a diameter of 8-10 mm, and 5 out of 30 cuttings with a diameter greater than 10 mm produced new stems of 10 cm or more along with leaves. The success rate of cuttings propagation was 11.3% of all cuttings and 15% of cuttings with a diameter greater than 8 mm. The results of this study show the possibility that cuttings using Magnolia obovata branches cut by pruning can be a viable propagation method.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.220-227
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• 2007

Sweetpotato fields in Korea are highly infected with virus and virus like diseases that greatly diminish both yield and quality as indicated by field observations and laboratory tests. In order to solve this problem, there is an urgent need to produce and mass propagate virus-free planting materials for distribution to the farmers. These experiments were conducted, firstly, to determine the most appropriate culture media, nutrient solution, and cutting intervals to maintain growth and vigor of tissue cultured plantleta as mother plants for propagation in insect-proof greenhouse. And as a labor saving method, the production efficiency of plug trays for rapid propagation of stem cuttings as a source of planting materials was likewise evaluated. Results showed that plants grown in medium B supplied with 0.5 and 1.0 strength of MS nutrients had high growth rate, and 20-day cutting interval was the best. 72-plug tray was better than 128-plug. Secondly, it was to develop a technique for the production of first-generation seed roots using hydroponics cultivation system. The yield of virus-free plants propagated in the non-insect proof and open-field cultivation was 2,402 kg/10a, 6% higher than those in the insect-proof cultivation, and the rate of virus re-infection was 18% higher compared to 3.3% with insect-proof cultivation. Lastly, it was to investigate the growth performance of virus free plants in farmers' field. Differences were existed in the yield depending on the variety used, but virus free plants showed an increase of $6{\sim}24%$ over virus infected plants.