• International conference on construction engineering and project management
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• 2017.10a
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• pp.192-200
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• 2017

With the acceleration of construction industrialization, the buildings that China has adopted the construction of industrialization technology are increasing day by day, and Precast Concrete (PC) Structure technology is one of the main technologies of construction industrialization. Compared with the traditional cast-in-place concrete structure, PC structure is more conducive to shorten the construction period, reduce the number of construction workers and the site construction waste. Nevertheless, PC structure improves the requirements of hoisting machinery in the construction site, and the lay-out and selection of hoisting machinery become an important factor influencing the construction cost. The paper regards the typical tower crane in China as the research object, and establishes the time optimization model for the lifting scheme. The influence of the different precast rate on the selection of the tower crane is analyzed. This paper obtains the time variation of the tower crane under different precast rate, provides a theoretical basis for the design of precast concrete structures under the influence of assembly construction, and lays the foundation for the selection of tower crane under the precast rate.

• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.398-406
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• 2022

The outer membrane of Gram-negative bacteria is the outermost layer of cellular environment in which numerous biophysical and biochemical processes are in action sustaining viability. Advances in cell engineering enable modification of bacterial genetic information that subsequently alters membrane physiology to adapt bacteria to specific purposes. Surface display of a functional molecule on the outer membranes is one of strategies that directs host cells to respond to a specific extracellular matter or stimulus. While intracellular expression of a functional peptide or protein fused to a membrane-anchoring motif is commonly practiced for surface display, the method is not readily applicable to exogenous or large proteins inexpressible in bacteria. Chemical conjugation at reactive groups naturally occurring on the membrane might be an alternative, but often compromises fitness due to non-specific modification of essential components. Herein, we demonstrated two distinct approaches that enable site-specific decoration of the outer membrane with a fluorescent agent in Escherichia coli. An unnatural amino acid genetically incorporated in a surface-exposed peptide could act as a chemoselective handle for bioorthogonal dye labeling. A surface-displayed α-helical domain originating from a part of a selected heterodimeric coiled-coil complex could recruit and anchor a green fluorescent protein tagged with a complementary α-helical domain to the membrane surface in a site- and hetero-specific manner. These methods hold a promise as on-demand tools to confer new functionalities on the bacterial membranes.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.36 no.3
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• pp.291-297
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• 2008

The launch of the COMS by using Ariane-5 launch vehicle is introduced. First, the COMS is introduced briefly, and then, the Ariane-5 launch vehicle is introduced including detail description of the improvement of Vulcain-1 engine of Ariane-5G to Vulcain-2 engine of Ariane-5ECA for 20% increase of thrust. Then, the launch process of the COMS is introduced. The COMS will be launched from the Guiana Space Center in Kourou, French Guiana. After the final check at PPF the COMS is transferred to HPF in the same building for fueling, and it is integrated to the launch vehicle adaptor at HPF, too. Then, this assembly is transferred to Final Assembly Building. After the satellites to be launched together are integrated to the launch vehicle on the launch table in the Final Assembly Building, the launch table loaded with the launch vehicle is moved to the launch pad for launch. The events during the launch vehicle flight is also introduced.

• BMB Reports
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• v.28 no.4
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• pp.336-341
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• 1995

Using a mutant M13 phage derivative lacking a great part of the complementary strand synthesis origin, we identified six single-strand initiation (ssi) signals for DNA replication in pACYC184, pLG214, pGKV21, and pDPT270 plasmids, and named them $ssiA_{YC}$, $ssiA_{LG}$, $ssiB_{LG}$, $ssiA_{KV}$, $ssiA_{PT}$, and $ssiB_{PT}$, respectively. Two of them were from pDPT270, one from downstream the on of pACYC184, two from pLG214, one from upstream the plus origin of pGKV21. Introduction of these ssi signals into the deleted $ori_c$ site of a mutant filamentous M13 phage ($M13{\Delta}lac182$) resulted in the restoration of growth activity of this phage. These ssi signals were classified into a number of groups on the basis of sequence similarity. $ssiA_{YC}$ and $ssiA_{LG}$ show extensive sequence homology to the n'-site (primosome assembly sites) of ColE1, whereas $ssiB_{PT}$ is homologous to the n'-site of ${\Phi}X174$. $ssiA_{PT}$ belongs to G4-type ssi signals which require only dnaG primase and SSB protein for the priming of replication. In addition, possible biological roles of these ssi signals are discussed.

• Journal of KSNVE
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• v.7 no.1
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• pp.33-41
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• 1997

To cover a range of possible site conditions where the Korean standard nuclear power plant may be constructed, a range of generic site conditions is selected for geologic and seismologic evaluation. To envelop other Asian countries as well as the Korean peninsula, there is an attempt to increase the seismic level to 0.3g ground motions for the safe shutdown earthquake. The dynamic analyses of the reactor vessel internals and fuel assemblies are performed for the increased motions and the effect of seismic level on the response is investigated. Also the nonlinear response characteristics are discussed by comparing the loads between operating basis earthquake and safe shutdown earthquake excitations. The design adequacy of the reactor vessel internals and fuel assemblies for the increased seismic level is addressed.

• Journal of Hydrogen and New Energy
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• v.20 no.5
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• pp.410-415
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• 2009

A hydrogen cooling method is used in a power generator for removing the unnecessary heat due to the windage loss of a rotor and the joule heat of a stator. A MEA (Membrane Electrolyte Assembly) hydrogen generator has been developed and applied as a hydrogen supplying system for the cooling of a 350MW power generator. As a field application result, the average potential of eleven cells and the voltage efficiency were measured 2.26V/cell and 65.4% (Higher Heating Value) respectively at the hydrogen pressure of 6 Bar, the hydrogen flow rate of 9.1L/min, and the current of 150A.

• Architectural research
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• v.20 no.3
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• pp.65-73
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• 2018

The restoration of foundations supporting the immense load of the stone pagoda at Mireuksa Temple Site prioritizes securing its structural stability. But so far, rammed earth construction is still not easy to determine the structural stability. This paper aims to emphasize that a scientific experimental study was conducted on a rammed earth construction, to identify its methodology and obtain objective data about structural stability of the foundation work. An experimental study fabricated specimens from the soil that had been removed during the excavation survey, determined the allowable bearing capacity through plate load tests, and compared the results with the predicted stress after reassembly of the stone pagoda to estimate the structural stability. Then, the repair method was selected based on the experimental study result. The evaluation method of the restoration of foundations consisted of an examination of the allowable bearing capacity and settlement. The allowable bearing of the reinforced foundation was more than twice the contact pressure under the stacked stones of the pagoda. The possibility of settlement of the rammed earth foundation soil layer during the pagoda assembly is expected to be very low because the settlement amount of the reformed soil layer is less than half of the settlement of the stabilized existing soil layer.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.301-301
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• 2011

We present theoretical investigations of the self-assembled growth of one-dimensional (1D) molecular lines directed across the dimer rows on the H-terminated Si(001) surface [1]. Based on density-functional theory calculations, a new growth mechanism of the 1D acetylacetone line is proposed [2], which involves the radical chain reaction initiated at two dangling-bond sites on one side of two adjacent Si dimers. It is also enabled that, if an H-free Si dimer were employed as the initial reaction site, a 1D acetylacetone line can grow along the dimer row. Our findings represent the first insight into the growth of 1D molecular lines not only across but also along the dimer rows on the H-terminated Si(001) surface.

• Steel and Composite Structures
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• v.21 no.2
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• pp.303-321
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• 2016

Tubular column members have been widely adopted in current construction due to its numerous advantages. However, the closed-section profile characteristics of tubular columns severely limit the connection possibilities. Welding type is acceptable but discouraged because of on-site issues. Blind-bolted connection is preferable because of its simplicity, economic benefit, and easy assembly. This paper presents a state-of-the-art review on bolted connections to tubular columns for bare steel tubes, including square and circular sections. Available studies on bolted connections at ambient and elevated temperatures are reviewed, but emphasis is given on the latter. Various methods of determining the connection performance through experimental, analytical, component based, and finite element approaches are examined. Future research areas are also identified.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2013.04a
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• pp.698-702
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• 2013

This paper considers the vertical pumps resonance and soundness. Normally large vertical pump's rotating speed is low, so the low natural mode can make a resonance on the motor and motor stand assembly. The pump resonance makes a very high vibration and trouble on the pump systems. Thus to avoid pump resonance when the pump is on the resonance region, we give the added mass method and evaluate the structure soundness by computer simulation and test on the site. Furthermore we evaluate the modal sensitivity and expect running conditions by the using ISO10814.