• The Korean Journal of Physiology
• /
• v.29 no.2
• /
• pp.269-277
• /
• 1995

Membrane vesicles were prepared by differential centrifugation from epithelial cells of porcine trachea. Total activity of microsomal ATPases was measured spectrophotometrically by a coupled enzyme assay. The steady-state activity of the enzyme was $329{\pm}10$ nmol/min mg protein. Thapsigargin, a specific antagonist of intracellular $Ca^{2+}-ATPase$, inhibited about 50% of the activity, leaving $178{\pm}18\;nmol/min .mg$ protein (n=6), indicating that the $Ca^{2+}-ATPase$ is one of the major microsomal ATPases. The microsomes used in this study appeared to be tight-sealed vesicles since they showed saturation in $^{45}Ca^{2+}$ uptake experiments. Inositol 1,4,5-trisphosphate $InsP_{3}, 4\;{\mu}M$, an agonist of $InsP_{3}$-sensitive $Ca^{2+}$ release channel ($InsP_{3}$, receptor), and Ca-ionophore A23187 $(10\;{\mu}M)$ induced $^{45}Ca^{2+}$ releases of 20% and 50% of stored $^{45}Ca^{2+}$, respectively. The addition of $(10\;{\mu}M\;InsP_{3}$ also increased the microsomal ATPase activity from $282{\pm}8$ nmol/min mg protein to $334{\pm}21$ nmol/min . mg protein in the intact vesicles. Similar increase in the activity was observed by making microsomes leaky (uncoupling) using the Ca-ionophore A23187. ;$InsP_{3}-induced$ effects were blocked by either thapsigargin or heparin suggesting that: 1) the $InsP_{3}-induced$ increase in ATPase activity is mediated by microsomal $Ca^{2+}-ATPase$, and 2) dissipation of $Ca^{2+}$ gradient across the microsomal membrane is responsible for the $InsP_{3}-induced$ effect. In order to test the dependence of the $Ca^{2+}-ATPase$ activity on the activity of $InsP_{3}-induced$ the activity of ATPases was monitored in various concentrations of free $Ca^{2+}$ using $EGTA-Ca^{2+}$ buffers. The $Ca^{2+}$-dependent biphasic change is the well-known character of $InsP_{3} receptor but not of microsomal $Ca^{2+}-ATPase$ in non-excitable cells; however, the activity of microsomal ATPase appeared biphasic and a maxim진 activity of $397{\pm}36nmol/min\;.mg$ protein was obtained in the solution containing 100 nM free $Ca^{2+}$. Below or above this concentration, the activity of ATPases was lower. These results strongly support a positive correlation of microsomal $Ca^{2+}-ATPase$ to the $InsP_{3}$ receptors in epithelial microsomes.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.31 no.1
• /
• pp.74-83
• /
• 1995

To estimate the parameters of a mathematical model of fishes' swimming behavior, the behavior in a experimental water tank was observed and analyzed using the video monitoring system. The tank was equipped with vertical circulation system, and measured $3,500L\;{\times}\;1,500B\;{\times}\;1,000H\;mm$ at flow channel and $1,200L\;{\times}\;900B\;{\times}\;500H\;mm$ at observational part. Rainbow trout, salmo gairdnerii were used as experimental fishes. Their swimming behavior in the tank was observed by the monitoring system, and the positions of every individual were checked at 0.5 second intervals by the image processing of recorded pictures for 5 minutes. The mean swimming speed calculated from the time series data of positions of every individual ranged from 2.5BL cm/sec to 2.9BL cm/sec at the stagnated flow. The mean swimming speed of 10 individuals in a school increased according to the flow speed. The mean swimming depth ranged from 17 cm to 38 cm even though it changed irregularly at the stagnated flow and gradually became stable according to the increase of flow speed. In the present study, the mean distance of individuals from wall of the tank varied from 17.6cm to 21.4cm. The mean distance between the nearest individual varied from 0.4BL cm to 0.7BL cm when 10 individuals in a school were observed. The mean dimension of fish schools became enlarged in all directions according to increase in the number of individuals, and as flow speed increased the horizontal dimension of fish schools expanded while their vertical dimension decreased.

• Journal of the Korean Society of Fisheries and Ocean Technology
• /
• v.42 no.3
• /
• pp.134-147
• /
• 2006

A model experiment using circulation water channel was carried out to investigate the dynamic characteristics of bottom trawl net which can be used in sea mount of North Pacific. Hydrodynamic resistance and shape variation according to the flow velocity and angle of hand rope transformation for net were measured, and experimental value was analyzed as the value of full-scale bottom trawl net. The results summarized are as follows; At the $30^{\circ}$ of angle of hand rope to net, hydrodynamic resistance varied from 0.5kgf to 2.68kgf as the flow velocity increased between 0.31m/s and 0.92m/s, and formula of hydrodynamic resistance for the model net was $F_m=3.04\;{\cdot}\;{\upsilon}^{1.53}$. At the fixed angle of hand rope, Net height was low and Net width was high according to the increase of flow velocity, and in addition, vertical opening was low and Net width was high by the increase of angle of hand rope at the fixed flow velocity. At the $30^{\circ}$ of angle of hand rope to net, net opening area was $0.214m^2$ as flow velocity was 0.61m/s, and formula of net opening area for the model net was $S_m=-0.22{\upsilon}+0.35$. At the $30^{\circ}$ of angle of hand rope to net, catch efficiency seemed to be highest as $0.319m^3/s$ of filtering volume at the 0.76m/s(51kt's) of flow velocity. Shape variation of net showed the gradual laminar transform for the variation of flow velocity but there needed some improvements due to the occurrence of shortening at the ahead of wing net.

• Journal of the Korean association of regional geographers
• /
• v.13 no.5
• /
• pp.509-525
• /
• 2007

The aim of this study is to evaluate the governance system involved in the policy of upgrading the Seongseo industrial complex in Daegu city. The governance system was evaluated by four indicators; social legitimacy, reliability, expertness, and transparency. The major findings of this study are summarized as follows. It is expected that the Seongseo industrial complex upgrading policy would accelerate to upgrade the industry structure and competitiveness of the cluster. However, the firms of the cluster have a Question of reliability to the actors of initiating the policy. In this context, to raise the trust level the policy needs to turn towards making the communication channel among the stake-holders of the cluster more democratically and horizontally. It is also problematic to reveal the low degree of expertness of the policy's decision makers, the fragmented system of policy operation and a lack of information. Consequently, the policy is required to improve the transparency on the policy-making process, and to activate the participation of professional groups and civil society.

• Analytical Science and Technology
• /
• v.26 no.4
• /
• pp.262-267
• /
• 2013

Gravitational field-flow fractionation (GrFFF) is a separation technique that utilizes earth's gravity as the external field. GrFFF is a convenient tool for the size and/or density-based separation of micron-sized particles of various origins. In this study, GrFFF was employed for size-based separation of oyster mushroom spores. Oyster mushroom spores have smooth surface and are of cylindrical to narrow kidney-shapes with 5 to 12 im in longer dimension and 3 to 4 im in shorter dimension, as was confirmed by optical microscope (OM). GrFFF conditions were optimized for separation and characterization of spores by varying the channel flow rate from 0.5 to 1 mL/min. During the GrFFF elution of the spores, 3 fractions were collected to confirm the growth of oyster mushroom spore. The collected fractions were incubated for 30 days in water to examine the influence of the size on the growth of the spores. Results suggested that the oyster mushroom spores collected at the middle part of the GrFFF fractogram grew faster than those collected at the beginning or at the end of the fractogram.

• The Korean Journal of Physiology and Pharmacology
• /
• v.15 no.1
• /
• pp.17-22
• /
• 2011

Quercetin mainly exists in the skin of colored fruits and vegetables as one of flavonoids. Recent studies show that quercetin, like other flavonoids, has diverse pharmacological actions. However, relatively little is known about quercetin effects in the regulations of ligand-gated ion channels. In the previous reports, we have shown that quercetin regulates subsets of homomeric ligand-gated ion channels such as glycine, 5-$HT_{3A}$ and ${\alpha}7$ nicotinic acetylcholine receptors. In the present study, we examined quercetin effects on heteromeric neuronal ${\alpha}3{\beta}4$ nicotinic acetylcholine receptor channel activity expressed in Xenopus oocytes after injection of cRNA encoding bovine neuronal ${\alpha}3$ and ${\beta}4$ subunits. Treatment with acetylcholine elicited an inward peak current ($I_{ACh}$) in oocytes expressing ${\alpha}3{\beta}4$ nicotinic acetylcholine receptor. Co-treatment with quercetin and acetylcholine inhibited $I_{ACh}$ in oocytes expressing ${\alpha}3{\beta}4$ nicotinic acetylcholine receptors. The inhibition of $I_{ACh}$ by quercetin was reversible and concentration-dependent. The half-inhibitory concentration ($IC_{50}$) of quercetin was $14.9{\pm}0.8\;{\mu}M$ in oocytes expressing ${\alpha}3{\beta}4$ nicotinic acetylcholine receptor. The inhibition of $I_{ACh}$ by quercetin was voltage-independent and non-competitive. These results indicate that quercetin might regulate ${\alpha}3{\beta}4$ nicotinic acetylcholine receptor and this regulation might be one of the pharmacological actions of quercetin in nervous systems.

• The Korean Journal of Physiology and Pharmacology
• /
• v.13 no.3
• /
• pp.229-239
• /
• 2009

The aim of the present study was to examine the effect of provinol, which is a mixture of polyphenolic compounds from red wine, on the secretion of catecholamines (CA) from isolated perfused rat adrenal medulla, and to elucidate its mechanism of action. Provinol (0.3 ${\sim}$ 3 ${\mu}g/ml$) perfused into an adrenal vein for 90 min dose- and time-dependently inhibited the CA secretory responses evoked by ACh (5.32 mM), high $K^+$ (a direct membrane-depolarizer, 56 mM), DMPP (a selective neuronal nicotinic $N_N$ receptor agonist, 100 ${\mu}M$) and McN-A-343 (a selective muscarinic $M_1$ receptor agonist, 100 ${\mu}M$). Provinol itself did not affect basal CA secretion. Also, in the presence of provinol (1 ${\mu}g/ml$), the secretory responses of CA evoked by Bay-K-8644 (a voltage-dependent L-type dihydropyridine $Ca^{2+}$ channel activator, 10 ${\mu}M$), cyclopiazonic acid (a cytoplasmic $Ca^{2+}$-ATPase inhibitor, 10 ${\mu}M$) and veratridine (an activator of voltage-dependent $Na^+$ channels, 10 ${\mu}M$) were significantly reduced. Interestingly, in the simultaneous presence of provinol (1 ${\mu}g/ml$) plus L-NAME (a selective inhibitor of NO synthase, 30 ${\mu}M$), the CA secretory responses evoked by ACh, high $K^+$, DMPP, McN-A-343, Bay-K-8644 and cyclpiazonic acid recovered to the considerable extent of the corresponding control secretion in comparison with the inhibition of provinol-treatment alone. Under the same condition, the level of NO released from adrenal medulla after the treatment of provinol (3 ${\mu}g/ml$) was greatly elevated in comparison to its basal release. Taken together, these data demonstrate that provinol inhibits the CA secretory responses evoked by stimulation of cholinergic (both muscarinic and nicotinic) receptors as well as by direct membrane-depolarization from the perfused rat adrenal medulla. This inhibitory effect of provinol seems to be exerted by inhibiting the influx of both calcium and sodium into the rat adrenal medullary cells along with the blockade of $Ca^{2+}$ release from the cytoplasmic calcium store at least partly through the increased NO production due to the activation of nitric oxide synthase.

• Journal of Astronomy and Space Sciences
• /
• v.24 no.1
• /
• pp.69-78
• /
• 2007

The international EARTHSHINE mission is to measure 1% anomaly of the Earth global albedo and total solar irradiance using Amon-Ra instrument around Lagrange point 1. We developed a new ray truing based integrated end-to-end simulation tool that overcomes the shortcomings of the existing end-to-end performance simulation techniques. We then studied the in-orbit radiometric performance of the breadboard Anon-Ra visible channel optical system. The TSI variation and the Earth albedo anomaly, reported elsewhere, were used as the key input variables in the simulation. The output flux at the instrument focal plane confirms that the integrated ray tracing based end-to-end science simulation delivers the correct level of incident power to the Amon-Ra instrument well within the required measurement error budget of better than ${\pm}0.28%$. Using the global angular distribution model (ADM), the incident flux is then used to estimate the Earth global albedo and the TSI variation, confirming the validity of the primary science cases at the L1 halo orbit. These results imply that the integrated end-to-end ray tracing technique, reported here, can serve as an effective and powerful building block of the on-line science analysis tool in support of the international EARTHSHINE mission currently being developed.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.12
• /
• pp.5843-5848
• /
• 2011

To improve the efficiency of the channel spectrum and to reduce the power consumption of the system in EVRC, the voice signal is compressed and transmitted only when the user speaks to. In addition to this, voice frames are divided into three rates 1, 1/2 and 1/8 and each frame is handled differently. For example, we assumed that the input is silence region if the 1/8 rate is used. In this paper, the sections are firstly separated into the voiced speech signal region, unvoiced speech signal region, and silence region by using distribution characteristics of LSP parameters. Then the paper suggested to encode 1 rate for the voiced speech signal, 1/2 rate for the unvoiced speech signal region, 1/8 rate for the silence region. In other words, traditional way of transmission is used when sending full rate in the EVRC. However, when sending half rate, the voice is firstly distinguished between voiced and unvoiced. If the voice is distinguished as voiced, voice is converted into full rate before the transmission. If it is distinguished as silence, EVRC's basic rate is applied. In the experimental results with SNR, ASDM, transmission bit rate measurement, we have demonstrated that voice quality was improved by using the proposed algorithm.

• Journal of the Korean association of regional geographers
• /
• v.15 no.2
• /
• pp.226-249
• /
• 2009

This study aims to establish the regional mix types and models of place marketing strategy. For this purpose, eighty seven cases of place marketing in Gwangju-Jeonnam region during the last two years are reviewed, Twenty seven types of regional mix are abstracted according to space, theme, subject, target, and factor. There are five spatial types(urban mix, zoning mix, zoning urban mix, package urban mix, zoning mix, and space package mix), eight thematic types(culture mix, history mix, tourism mix, industry mix, administration mix, environment mix, transportation mix, and PR mix), five subject types(central government led public mix, local government led public mix, enterprise led private mix, civil society led private mix, and private public partnership mix), four target types(resident mix, tourist mix, enterprise mix, and common mix), and five factor types (organization mix, image mix, point mix, target mix, and channel mix). In the basis of these types, the twenty two primary model of regional mix, and the one hundred twenty six secondary model of regional mix are established.