• Korean Journal of Veterinary Research
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• v.15 no.2
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• pp.287-291
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• 1975

In order to observe the kinetic of ciliates inhabited in the stomach, investigation of the content juice in the stomach was carried out on the 30 Korean native cattle, selected as healthy ones and slaughtered at Jeonju abattoir in August, 1974. The following is a brief summary of the leading facts gained through the experiment. 1. The average pH of the content juice showed $6.567{\pm}0.056$(SE) in the rumen, $6.607{\pm}0.053$ in the reticulum, $6.183{\pm}0.056$ in the omasum and $2.627{\pm}0.061$ in the abomasum. 2. The presumed number of ciliates per ml was calculated $168,200{\pm}17,035$(SE) in the rumen, $173,200{\pm}19,728$ in the reticulum, $23.600{\pm}3.537$ in the omasum and $467{\pm}266$ in the abomasum. 3. In the content juice the number of ciliates was gradually slowed down, the pH decreased from rumen to abomasum and the changes were denoted by quadratic curves(parabolas). 4. In accordance with the increase of the number of ciliates the pall also becomes higher and in this situation the correlation coefficient was ${\gamma}_{xy}=0.508$. 5. Population density of ciliates was checked and Entodinium showed 60 per cent among them, but Polyplastron was figured only from 0 to 0.05 per cent. 6. Among 26 species of ciliates which were identified from the content juice, Entodinium simplex was showed the highest population density, raging from 40 to 50 per cent, Dasytricha ruminantium was neat to it, Eudiplodinium affine after the next, and Entodinium caudatum and Eudiplodinium maggi were also found abundantly in the abomasum.

• Korean Journal of Veterinary Service
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• v.22 no.4
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• pp.393-398
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• 1999

This case occurred following accidental consumptions of toxic levels of feedstuffs in the Korean indigenous cattle which were pregnant for 3-6 months. Grossly, poorly defined bluish coloration in the ventral sac of the rumen, reticulum and omasum was visible on the surface of the stomach. The severely affected ruminal walls were perforated and blood-tinged exudate was observed and death followed due to acute diffuse peritonitis. There were copious amount of porridgelike rumen contents, which has a distinct fermentative odor. The epithelia were easily wiped off and hyperemic in patch. Histopathologically, chemical rumenitis with neutrophilic infiltration and opportunistic fungal infection was evident in the mucosa and submucosa. Multifocal necrotic foci with fungal hyphae on the liver and severe congestion on the renal medulla were noted. There was no evidence of heavy metal and aflatoxin Bl in the suspected feedstuffs. This case was diagnosed as acute carbohydate engorgement in cattle.

• Korean Journal of Veterinary Research
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• v.33 no.4
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• pp.673-678
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• 1993

A male sika deer, 3 years old, which had the clinical history of chronic watery diarrhea with severe emaciation for 4 months was submitted and euthanized. On necropsy, there was diffuse mucosal hypertrophy of gastrointestinal tract with thickened intestinal walls and swollen mesenteric lymph nodes. In histologic lesions. diffuse proliferation of epithelioid cells are present in the laminar propria, submucosa muscle layer and subserosa of most gastroinlestinal organs except omasum. These epithelioid cells are also present in the cortex and medulla of meseriteric lymph nodes and in the portal triads of liver. Most these epithelioid cells contain acid-fast positive bacilli using Ziehl-Neelsen staining in the cytoplasm. Based on the clinical signs, gross findings and histological lesions, this deer was diagnosed as paratuberculosis. The wide range of target organs and the severity of the lesions observed in this case is quite different comparing to those of other ruminant.

• Journal of Animal Science and Technology
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• v.49 no.3
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• pp.341-350
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• 2007

The present study was conducted to measure diurnal patterns in the flow of soluble non-ammonia nitrogen (SNAN) fractions in the liquid phase of digesta entering the omasum of cows fed grass-red clover silage supplemented with barley and rapeseed meal. Four ruminally cannulated cows were fed, in a 4×4 Latin square design, grass-red clover silage alone (GS) or supplemented with (on a DM basis) 6.0kg/d of barley grain, 2.1kg/d of rapeseed meal or 6.0kg/d of barley and 2.1kg/d rapeseed meal. Omasal digesta was taken using an omasal sampling system at 1.5h intervals during a 12h feeding cycle, and SNAN fractions (free AA, peptide and soluble protein) in the omasal digesta were assessed using ninhydrin assay. Dietary supplementation numerically increased the mean flow of SNAN fractions relative to GS diet despite the lack of statistical significance. Diurnal patterns in the flow of peptide entering the omasum during a 12h feeding cycle appeared to be highest immediately after feeding, declined by 10.0h post-feeding and slightly increased thereafter. In SNAN fractions, the flow of peptide was higher for supplemented diets than for GS diet throughout the feeding cycle. Based on the microbial contribution to total SNAN using 15N, diurnal patterns in the flow of dietary SNAN for dietary supplemented diets appeared to be higher compared with GS diets. Present results may conclude that peptide flow is quantitatively the most important N in SNAN fractions and that dietary supplementation can increase peptide flow entering the omasal canal.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.1
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• pp.99-102
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• 1991

To determine effect of time after feeding on distribution of digesta in the gastro-intestinal tracts of sheep given orchardgrass hay once a day, a total of fifteen ewes (mean live weight $51{\pm}12kg$) were slaughtered at 2, 8, 16 and 24 hours after feeding. Contents in the reticulo-rumen, omasum, abomasums, small intestine, cecum, and colon and rectum were totally collected and weighed. Weights of digesta in the reticulo-rumen were about 6 kg which contributed about 75% of the total in the whole tracts. Digesta on dry-matter basis totaled about 1 kg. The dry-matter concentration of digesta in the whole digestive tract was about 107 h/kg of fresh digesta. Distribution of moisture in the digestive tract changed in parallel with that of fresh digesta. There was no significant correlation observed between time after feeding and weights of digesta in the gastro-intestinal tracts. While, feed intake significantly correlated with digesta in the reticulo-rumen, cecum and whole tracts (p<0.01). Thus, time after feeding was inferred to have no influence upon the content of digesta in the digestive tract, but feed intake influenced on the content of digesta in the digestive tract at a low level of feeding.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.8_spc
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• pp.1321-1330
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• 2019

Recent development of novel techniques in systems biology have been used to improve and manipulate the rumen microbial ecosystem and gain a deeper understanding of its physiological and microbiological interactions and relationships. This provided a deeper insight and understanding of the relationship and interactions between the rumen microbiome and the host animal. New high-throughput techniques have revealed that the dominance of Proteobacteria in the neonatal gut might be derived from the maternal placenta through fetal swallowing of amniotic fluid in utero, which gradually decreases in the reticulum, omasum, and abomasum with increasing age after birth. Multi "omics" technologies have also enhanced rumen fermentation and production efficiency of dairy goats using dietary interventions through greater knowledge of the links between nutrition, metabolism, and the rumen microbiome and their effect in the environment. For example, supplementation of dietary lipid, such as linseed, affects rumen fermentation by favoring the accumulation of ${\alpha}$-linolenic acid biohydrogenation with a high correlation to the relative abundance of Fibrobacteriaceae. This provides greater resolution of the interlinkages among nutritional strategies, rumen microbes, and metabolism of the host animal that can set the foundation for new advancements in ruminant nutrition using multi 'omics' technologies.

• Korean Journal of Veterinary Research
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• v.12 no.1
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• pp.127-132
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• 1972

Experimental studies on the water content of muscles and the histological changes of muscles, digestive tract and kidney, influenced by forced oral administration of excessive tap water, were performed by using 10 Korean bulls. Experimental animals were divided into two groups, five heads for test group and five for control group. All of the bulls used in this experiment were weighing between 250 kg and 300 kg and 3 and 4 years of age. In each test animal, a total of 140 litters of tap water was passed in the forestomach by means of catheterization within 12 to 18 hours. And each of them was slaughtered immediately after the animals showing symptoms of respiratory distress. In control group, the animals were allowed to drink tap water normally. From test and control animals after slaughter, each 10 gm of M. biceps femoris, M. satorius, M. adductor, M. gluteous supercialis, M. iliocostalis lumborum, and M. transversus costarum were taken from definite parts in order to measure water content. In the histopathological studies tissues of rumen, reticulum, omasum, abomasum, duodenum, colon and kidney were taken as wall as the above mentioned muscles. The results obtained were as follows; 1. Increase of water content in the muscles of test group were 4.6% in M. satorius, 4.24% in M. transversus costarum, 4.14% in M. gluteous supercialis, 4.02% in M. adductor, 3.88% in M. biceps femoris, and 2.46% in M. iliocostalis lumborum respectively. The highest increase was found in M. satorius and the lowest in M. iliocostalis lumborum. 2. In test animal, average increasing value of water content in muscles was 3.9% and shown highly significant (p<0.01). 3. On the microscopical findings of carcass, marked watery edema was observed all of the subcutaneous tissues and intermuscular connective tissues. 4. Microscopically, the epimysium, perimysium and endomysium were widened, and the muscle fibers were lacerated. The cells of stratum lucidum in the epithelium of rumen and reticulum were shown marked vacuolization. In the kidney, dilatation of Bowman's spaces and proximal tubles was observed.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1460-1468
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• 2003

An experiment was conducted to quantify the flow of soluble non-ammonia nitrogen (SNAN) in the liquid phase of ruminal (RD) and omasal digesta (OD), and to investigate diurnal pattern in SNAN flow in OD. Five ruminally cannulated Finnish-Ayrshire dairy cows in a $5{\times}5$ Latin square design consumed a basal diet of grass silage and barley grain, and that supplemented with four protein feeds (kg/d DM basis) as follows: skimmed milk powder (2.1), wet distiller' solubles (3.0), untreated rapeseed meal (2.1) and treated rapeseed meal (2.1). Ruminal digesta was sampled using a vacuum pump, whereas OD was collected using an omasal sampling system at 1.0 h interval during a 12 h feeding cycle. Both RD and OD were acidified, centrifuged to remove microbes and precipitated with trichloroacetic acid followed by centrifugation. The SNAN fractions (free amino acid (AA), peptide and soluble protein) in RD and OD were assessed using ninhydrin assay. Free AA, peptide and soluble protein averaged 60.0, 89.4 and 2.1 g/d, respectively, for RD, and 81.8, 121.5 and 2.5 g/d, respectively, for OD. Although free AA flow was relatively high, mean peptide flow was quantitatively the most important fraction of SNAN, indicating that degradation of peptide to AA rather than hydrolysis of soluble protein to peptide or deamination may be the most limiting step in rumen proteolysis. Diurnal pattern in flow of peptide including free AA in OD during a 12 h feeding cycle peaked 1 h post-feeding, decreased by 3 h post-feeding and was relatively constant thereafter. Protein supplementation showed higher flow of peptide including free AA immediately after feeding compared with no supplemented diet. There were no differences among protein supplements in diurnal pattern in flow of peptide including free AA in OD.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.695-703
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• 1994

The development of reticulum in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by light, scanning electron microscopy. The results were summarized as follows; 1. In the 60-day-old fetuses, the stomach was developed and differentiated into four compartments of rumen, reticulum, omasum, and abomasum. The reticular epithelial layers were differentiated into two zones; a small dark basal and a large light luminar zones. The wall of reticulum resembled that of the rumen except that the mucosa was in the cranio-dorsal region of the reticulum. 2. In the 90-day-old fetuses, the light luminar zone of the reticulum was about 10-16 times thicker than the dark zone. The outlines of the reticular ribs were visible. 3. In the 120-day-old fetuses, the wall of the reticlum had also increased in thickness. The reticular mucosa exhibited an irregular luminar surface and the invaginations had differentiated into large regularly arranged ones separated by 3-5 and small irregularly arranged ones. 4. In the neonate, the luminar surface of the reticular mucosa demonstrated clear furrows, at which the superficial cells of the light zone had undergone degenerative changes. 5. Scanning electron microscopic studies; In the 60-day-old fetuse, numerous microvilli were observed on the superficial epithelial layer of shape or dome like at 120 days. In the neonate, the reticular papillae liked the little finger.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.687-694
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• 1994

The development of rumen in fetuses between 60, 90, 120 days of gestation and neonates of Korean native goats was investigated by light, scanning electron microscopy. The results were summarized as follows; 1. In the 60-day-old fetuses, the stomach was developed and differentiated into four compartments of rumen, reticulum, omasum, and abomasum. The ruminal epithelial layers were differentiated into two zones; a small dark basal and a large light luminar zones. 2. In the 90-day-old fetuses, the light luminar zone of the rumen was 6-12 times thicker than the dark zone. 3. In the 120-day-old fetuses, the wall of the rumen had increased in thickness, and its the mucosa was still smooth. Several undulations of various depths involving the basal zone, basement membrane and lamina propria were observed at irregular within the light zone. 4. In the neonate, the wall of the rumen had increased in thickness and the luminar surface had become wavy due to the appearance of several shallow furrows in the luminar surface of the epithelium especially between the tips of the relatively more developed undulations. 5. Scanning electron microscopic studies: In the 90-day-old fetuses, numerous microvilli and microridges were observed on the superficial epithelial layer of the rumen. The ruminal papillae were already formed at 120 days of gestation. In the 120-day-old fetuses, the ruminal papillae formed short dome-shape. In the neonate, the ruminal papillae formed pebble-shape or short finger like.