• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1566-1573
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• 2009

Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-$\gamma{2}$ and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore, trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.

• 한국수산과학회지
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• 제16권4호
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• pp.395-400
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• 1983

1982년 11월 18일 부터 25일 사이에 해운대 동백섬에 위치한 부산수산대학 해양과학연구소 앞 연안에서 주낚으로 채집된 성숙한 노래미를 이용하여 실험실내에서 인공수정에 의한 난발생과정과 부화자어에 관하여 관찰한 결과를 요약하면 다음과 같다. 난은 구형이며 침성점착난으로 난경은 $1.92{\sim}2.14mm$로 짙은 황색의 유구가 무수히 존재한다. 실내수온 $11.8{\sim}17.9^{\circ}C$(평균 $14.2^{\circ}C$)에서 수정후 463시간만에 부화하였다. 부화직후의 자어는 전장 $6.55{\sim}8.2mm$이며 근절수 12+36=48. 난황의 전단에 큰 유구가 하나 있다. 부화후 3일째의 전장 7.8mm의 자어는 꼬리부분의 $13{\sim}19$번째의 근절부분에 $4{\sim}5$개의 흑색소포 나타난다.

• 공업화학
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• 제3권2호
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• pp.349-359
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• 1992

염료속에 함유된 무기염(NaCl, $Na_2SO_4$)의 선택적 제거를 위해 역확산과 역삼투를 결합한 방법과 nanofiltration(NF)을 각각 이용하였다. 역확산에 의한 염의 제거율은 염의 종류에 따라 1회(one pass)에 1~30%를 나타냈으며 염과 염료의 분리비는 10~500의 매우 큰 값을 갖는 반면 염료의 손실율은 0.3% 이하였다. 염료용액을 순환시킬수록 음이온인 $Cl^-$ 이온의 제거율이 증가하고 양이온인 $Na^+$ 이온의 제거율이 감소하는 도난투석현상이 관찰되었다. 또한 공급용액의 유속이 염의 제거에 미치는 영향을 살펴보았다. 역확산에 의해 염이 제거된 염료를 평판형 역삼투막을 사용하여 농축하였으며 $Cl^-$ 이온의 배제율을 solution-diffusion 모델식에 적용하였다 2회의 Diafiltration(DF)을 수행한 NF에서도 역확산에서와 마찬가지로 도난투석에 의해 $Cl^-$ 이온의 배제율 감소와 음배제율을 관찰하였다. 특히 두번째 DF에서 도난투석의 효과는 더욱 크게 나타났다.

• 대한미생물학회지
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• 제13권1호
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• pp.25-30
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• 1978

Serratia marcescens의 동정(同定)을 분리배지(分離培地)를 이용(利用)하여 간편(簡便)한 방법(方法)으로 시도하였던 바 prodigiosin 산생(産生)은 proline을 위시해서 각종(各種) amino acid, thiamine 및 nicotinic acid에 의해서 항진되지 않았으나 혈액평판배지(血液平板培地)에서 본(本) 균종(菌腫)은 시간(時間)의 경과(經過)에 따라 흑색조(黑色調)를 띤 집락(集落)을 형성(形成)하며 이는 일차적(一次的)인 분리기준(分離基準)으로 이용(利用)될 수 있을 것 같다. Tween 80 및 대두유(大豆油) 분해성(分解性은) 본(本) 균(菌)에서 양성(陽性)이며 동정(同定)에 이용(利用)될 수 있으나 특이반응(特異反應)이 아니다. 그러므로 마지막으로 oxidase 반응(反應), lactose 및 sucrose 발효시험이 본(本) 균종(菌腫)의 확정(確定)에 수반되어야 한다.

• 식물병연구
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• 제11권2호
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• pp.167-172
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• 2005

오이 흰가루병균의 생물적 방제제로 선발한 중복기생균 Ampelomyces quisqualis 94013의 오이 흰가루병 균에 대한 기생력을 온도와 습도에 대하여, 그리고 mineral oil 등 10종의 전착제와 혼용하였을 때 기생력 증진효과를 검토하였다. 또한 A. quisqualis 94013균주의 기주범위에 대하여 오이, 호박, 참외, 멜론, 수박, 박, 딸기, 가지, 토마토, 콩, 팥, 장미에 기생하는 흰가루병균 Sphaerotheca속 6종, Erysiphe속 1종에 대하여 조사하였다. 오이 흰가루병균에 대하여 A. quisqualis 94013의 기생이 가능한 온도 범위는 $12\~30^{\circ}C$이고, 기생 최적온도는 $20\~28^{\circ}C$이였다. A. quisqualis 94013균주는 $20\~35\%$의 저습도에서 오이흰가루병균에 대하여 기생력이 저조하였다. 또한, A. quisqualis 94013의 기주범위조사에서 오이, 멜론, 수박, 참외, 박, 딸기, 토마토, 가지, 장미 등 12종의 작물을 침해하는 흰가루병균 Sphaerotheca속 6종, Erysiphe속 1종에 대하여 기생성이 있어 기주특이성이 없는 것으로 나타났다.

• 공업화학
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• 제25권6호
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• pp.553-558
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• 2014

미세조류는 통상적인 에너지 작물에 비하여 빠른 성장속도와 높은 오일함량으로 바이오디젤 생산의 원료로 관심을 받고 있다. 미세조류의 지질은 주로 트리글리세라이드인 중성지방으로 에스테르교환반응을 통하여 바이오디젤인 지방산 메틸에스테르로 전환할 수 있다. 본 논문에서는 영양분의 제한, 염도 및 금속 성분의 변화와 같이 미세조류의 지질 생산을 촉진할 수 있는 배양환경 스트레스 조건의 영향들을 비교 고찰하였다. 사용하는 미세조류 종에 따라 스트레스에 대응하여 지질의 양이 변하거나 구성하는 지방산의 조성이 변화될 수 있다. 비록 질소원 제한 조건이 가장 많이 사용되는 지질생산 촉진조건이긴 하지만, 미세조류로부터 바이오디젤 생산성의 향상을 위해서는 그 외에도 영양분 과잉 조건, 염도의 변화, pH, 온도, 금속 성분 농도 변화 등의 다른 조건들도 고려되어야 한다.

• 대한한의학회지
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• 제18권1호
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• pp.187-197
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• 1997

Major separation for the active ingredients and characterization of chemical properties in conjunction with screening test on animal were performed in order to analyze and standardize Ligustici Rhizoma or Angelicae Tenuissimae Radix as an important oriental herbal medicine for antiphlogistic or an important oriental herbal medicine for antiphlogistic or an anodyne. Furthermore the structure, composition and contents of ingredients for essential oil in Angelicae Tenuissimae Radix(Suckpo, Korea) were determined by means of Ge/MS followed by screening test on Z-ligustilide(82%) known as major ingredient as well as butylidenephthalide collected by HPLC with normal phase semiprep-column. The total active ingredient in Ligustici Rhizoma from China or Angelicae Tenuissimae harvested at Choonyang(Kyungnam, Korea), Jungsun(Kangwon, Korea), Suckpo(Kyungnam Korea), Youngchun(Kyungnam, Korea) have been determined showing higher abundant for three times on the product in Korea compared to that in China. In addition, the major component in Ahgelicae Tebyussunae Radux extract was found to be Z-ligustilide(70-80%) which is very different from that in Ligustici Rhizoma senkyunolide(39%) as major species. For screening test of Ligustici Rhizoma or Angelicae Tenuissimae Radix extracts toward the target animal, the efficiency has been shown the similarity on both extracts. Taking into account the level of ingredient, the total efficiency may be three times higher on Angelicae Tenuissimae Radix in Korea compared to Ligustici Rhizoma in China. As a result of present study, it is preferable to distinguish between Ligustici Rhizoma and Angelicae Tenuissimae Radix for better usage of oriental herbal medicine because of very different composition and abundant in spite of their similar screening effect.

• Animal cells and systems
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• 제15권3호
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• pp.211-218
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• 2011

Fragmentation in human pre-implantation embryos has been suggested as the process of apoptosis. We have previously demonstrated a direct relationship between the increased reactive oxygen species (ROS) and apoptosis in human pre-implantation embryos. ROS is known to suppress the function of mitochondria in which steroidogenic acute regulatory protein (StAR) and peripheral-type benzodiazepine receptor (PBR) are presented. Therefore, the purpose of this study was to examine the expression of StAR and PBR in human pre-implantation embryos and to evaluate whether reduction of these proteins is associated with apoptosis. Apoptosis was detected by annexin V-fluorescein isothiocyanate (FITC) and mitochondrial membrane potential was measured by 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazolyl-carbocyanine iodide (JC-1). Immunofluorescence staining and Western blotting were applied to examine the expression of StAR and PBR in the embryos. Lipid droplets in the embryos were stained with Oil Red O. The fragmented pre-implantation embryos were stained with annexin V-FITC, but not the normal ones. The mitochondria with active membrane potential were present less in the fragmented embryos compared with the non-fragmented embryos. We also confirmed that both StAR and PBR were expressed in the embryos and their expression levels were lower in the fragmented ones. In addition, the number and size of lipid droplets were increased in the fragmented embryos. The present study provides evidence that reduction of StAR and PBR in human pre-implantation embryos is associated with an increase in the lipid droplets leading to apoptosis.

• 대한화장품학회지
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• 제22권2호
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• pp.41-51
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• 1996

Phaseolus aureus (mung bean), Leguminosae, has been used as an antidote from the ancient time. Especially, it has been widely used for cleaning face and skin in oriental countries. Although several constituents such as fatty acids, phytoallexin and phaseol derivatives were reported in P. aureus and related species including seedlings, there has been a few report to describe its biological activity. Therefore, in this investigation, the ethanol extract from P. aureus was obtained and its biological activities including the antioxidative and anti-inflammatory activities were studied. The 70% ethanol extract from P. aureus showed dose-dependent antioxidative activity (52.3% inhibition at 4 mg/ml) against lipid peroxidation assay, while the extract did not show the inhibitory activity of superoxide radical formation. The extract also showed the topical anti-inflammatory activity against croton-oil and arachidonic acid induced mouse ear edema test (18-19% inhibition at 7.5 mg/ear) as well as mild inhibitory activity against picryl chloride induced delayed hypersensitivity in mouse. For investigating active principles, vitexin and isovitexin (apigenin C-glycoside) as flavonoids, and adenosine were isolated from the extract using silica gel chromatography. The actual contents of vitexin and isovitexin were found to be 3.7 and 2.4 mg/g extract, respectively. Vitexin and isovitexin showed the antioxidative activity. They showed the topical anti-inflammatory activity, although the activities were not potent compared to the reference compounds. These results suggested that vitexin and isovitexin may be, at least in part, the compounds contributing the antioxidative activity in vitro and the topical anti-inflammatory activity of P. aureus in vivo. All results of present study might be one of the scientific rationale in using mung bean for skin care from the ancient time.

• Bulletin of the Korean Chemical Society
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• 제31권11호
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• pp.3327-3332
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• 2010

We investigated the effects of newly synthesized 4-[(butylsulfinyl)methyl]-1,2-benzenediol (SMBD) on the prevention of atherosclerosis in hypercholesterolemic rabbits. SMBD exhibited stronger inhibition of $Cu^{2+}$-induced low-density lipoprotein oxidation than that of ascorbic acid or simvastatin. Three-month-old rabbits were fed an atherogenic diet containing 0.5% cholesterol and 10% coconut oil, while other two groups were given an atherogenic diet with intravenous injection of either simvastatin or SMBD (0.33 mg/kg/day) for 4 weeks. The concentrations of plasma cholesterol and thiobarbituric acid reactive substances were significantly decreased in SMBD groups, compared to the control group. Also, aortic lipid level in the SMBD group significantly lower than that in the control group. Furthermore, compared with the control group, the SMBD group significantly inhibited the increase of aortic intimal thickness by 36% via reducing of aortic reactive oxygen species and cyclooxygenase-2 protein levels. We conclude that raised antioxidant effect of SMBD results in significant prevention against hypercholesterolemia.