• Applied Biological Chemistry
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• 제36권5호
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• pp.332-338
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• 1993

리보플라빈을 생산하기 위하여 Ashbya gosspii NRRL Y-1056의 균주개량을 시도하였으며 선발한 변이주 Ashbua gossypii JAG-13에 의한 리보플라빈의 생산시의 최적 배지조성 및 배양조건을 결정하였다. 최적 배지조성은 9%의 옥수수 기름, 3%의 젤라톤, 4%의 CSL, 0.3%의 글리신, 0.2%의 자당 지방산 에스테르 S770 였다. 배양배지의 초기 pH와 최적 배양온도는 각각 pH 6.5 와 $28^{\circ}C$였다. 산소의 공급은 리보플라빈의 생산에 필수적이었으나 과도한 산소의 공급은 오히려 리보플라빈의 생산을 저해하였다. Ashbya gosspii JAG-13을 위와같은 조건에서 생물배양기를 이용하여 12일간 배양하여 6.9 mg/ml의 리보플라빈을 생산하였다.

• 한국양식학회지
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• 제18권4호
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• pp.267-271
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• 2005

A feeding trial was carried out to investigate the effect of various fish meals as dietary protein source on growth, feed utilization and body composition of Juvenile flounder. Ten experimental diets were prepared to contain different fish meals: 4 kinds of white fish meal (WM-1, 2, 3, 4), 3 kinds of herring meal (HM-1, 2, 3), mackerel meal (MM), WM mixture and HM+MM mixture. Dietary energy and protein levels were designed to be isocaloric ($3.8 kcal g^{-1}diet$) and isonitrogenous ($46\%$) by adjusting the levels of fish meal, wheat flour and squid liver oil. Three replicate groups of fish (initial mean weight: 11.1 g) were hand-fed to visual satiety two times daily for 7 weeks. Survival was not significantly different among all groups. Weight gain of fish fed the HM-1 and HM-3 diets was the highest, but not significantly different from that of fish fed the either WM-2 or HM+MM diets. The lowest weight gain was found in fish fed the W-1 and WM-3 diets. Feed efficiency and protein efficiency ratio of fish fed the WM-2, HM-1, HH-3, MM and HM+MM diets were significantly higher than those of fish fed other diets. Daily feed intake of fish fed the WM-3 diet was highest, but not significantly different from that oi fish fed the either WM-4 or WMM diet. Significant differences were found in contents of moisture, crude protein, crude lipid and ash of whole body of fish. The results of this study indicated that growth of flounder can be affected by dietary fish meal source and quality, and WM-2, HM-1, HM-3 and HM+MM mixture are considered as useful dietary fish meal sources under these experimental conditions.

• 한국수산과학회지
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• 제15권1호
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• pp.94-98
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• 1982

낙동강 하류산 재첩 (Corbicula elatior)에 함유되어 있는 지질의 이화학적 성질, 조성 그리고 이 지질의 지방산과 sterol 조성을 밝히기 위하여 실험한 결과는 다음과 같다. (1) 조지질과 불연화물 함양은 각각 $2.17\%,\;16.6\%$였으며 요-드가는 119였다. (2) 조지질의 조성은 린지질이 약 $43\%$로 가장 많았고 triglyceride (약$40\%$), 유리 sterol ($11\%$)의 순이었다. (3) 지방기조성은 $C_{16:0}$가 $48.4\%$로 가장 많았고, C_{14:0}\;(10.0\%, C_{14:1}\;5.4\%,\;C_18:1 (5.3\%)의 순이였으며 $C_{19:0}$으로 생각되는 지방산이 $6.4\%$였다. (4) 6종의 sterol 이 검출되었는데 24-nlethylene-cholesterol이 $41\%$로 가장 많았고 $\beta-sitosterol(22.5\%)$, fucosterol이 $14.9\%$였으며 isofucosterol과 과 $\Delta^7-avenasterol (?)(5.2\%)$로 생각되는 것이 각각 $14.0\%$, $5.2\%$ 였고 cholesterol은 $2.3\%$로 가장 적었다.

• Applied Biological Chemistry
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• 제23권1호
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• pp.14-22
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• 1980

흰참깨에 20.5%, 검정참깨에 19.20%, 참박(粕)깨에 44.7%의 조단백질이 함유되어 있다. n-hexane을 용매로하여 지방을 추출한 것이 수율이 가장 높았다. 조단백질의 분리에 있어서 pH가 알칼리성으로 갈수록 수율이 좋았다. 참깨의 globulin은 70.9%이었고 prolamin는 1.6%였다. 전(全)참깨의 아미노산으로는 glutamic acid가 17.1%로 가장 많았고 globulin의 아미노산으로는 glutamic-acid 14.6%이었고 필수 아미노산도 다량 고루 함유되어 있었다. 흰참깨와 검정참깨의 albumin 및 globulin의 단백질은 Disc gel 전기영동상에서 각각 $12{\sim}13$개 그리고 2개의 band로 나타났다. 참깨박(粕) 단백질의 주단백질인 globulin의 Sephadex G-100 및 G-200 column 분리하고 이를 disc gel 전기영동으로 검색하였다.

• 한국안광학회지
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• 제8권2호
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• pp.25-29
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• 2003

자외선과 전자파를 동시에 차단하는 선글라스렌즈를 제작하기 위하여 유리가판 위에 자성재료인 페라이트에 Ni를 여러 가지 조성비로 첨가한 니켈페라이트 박막 $Ni_xFe_{3-x}O_4$을 페라이트 도금법으로 제작하였다. 이들은 모두 스피넬 구조의 단일상임을 확인할 수 있었으며 육안으로 관찰할 때 거울면과 같은 광택을 가졌으며, 손톱으로 긁었을 때 흠집이 생기지 않는 강도를 보였다. 니켈페라이트는 400nm 부근에서부터 급격히 투과율이 떨어져 자외선을 차단하는데 효과적인 양상을 보이고 있으며 그 중에서 x = 0.09의 Ni 조성비가 자외선 차단효과가 가장 좋은 깃으로 나타났다. 이는 타사제품과 비교하여 자외선 차단효과는 크게 떨어지지 않으므로, 전자파 차단효과가 있는 $Ni_xFe_{3-x}O_4$ 페라이트를 코팅한 선글라스를 착용함으로서 유해전자파와 자외선을 동시에 차단하는 효과를 얻을 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권6호
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• pp.873-879
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• 2003

The study was conducted to improve the nutritive value of defatted rice polishing (DRP). DRP was treated with various concentrations of HCl, NaOH, $H_2O_2$ and Kemzyme-H $F^{(R)} and the effect on its chemical composition and nutritive value in broiler chicks was observed. The treatments levels of 0.4 N HCl, 0.2 N NaOH and 6% $H_2O_2$ were selected from many concentrations of HCl, NaOH and $H_2O_2$ tried earlier on DRP. The selection was made on the basis of release of nutrients from DRP. The Kemzyme-H $F^{(R)} was used at rate of 0.1% of DRP. The selected concentrations of HCl, NaOH, and $H_2O_2$ were then used for treatment of DRP that was used in biological experiments. Two hundred and forty, day-old Hubbard male broiler chicks (38-40 g) were randomly divided into 48 experimental units with five chicks each. Each chemically treated DRP was incorporated into broiler diets at 10, 20 or 30% levels replacing yellow corn from the control feed and thus sixteen experimental feeds were prepared. These feeds were randomly assigned to 48 experimental units such that there were three replicates of chicks on each diet. The results of the study suggest that DRP can be effectively used in broiler diets at 20% level. The best weight gain and feed conversion ratio were observed with diet containing 20% level of DRP treated with 6% $H_2O_2$. The diets containing 30% levels of treated DRP were uneconomical, as excess use of oil was required to compensate the energy needs of the birds.

• Asian-Australasian Journal of Animal Sciences
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• 제26권3호
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• pp.378-385
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• 2013

The aim of this study was to examine the effect of variety and growing conditions of wheat on broiler performance and nutrient digestibility. One hundred and sixty-four wheat samples, collected from a wide range of different sources, locations, varieties and years, were analyzed for a range of chemical and physical parameters. Chemical and physical parameters measured included specific weight, thousand grain weight (TG), in vitro viscosity, gross energy, N, NDF, starch, total and soluble non-starch polysaccharides (NSP), lysine, threonine, amylose, hardness, rate of starch digestion and protein profiles. Ninety-four of the wheat samples were selected for inclusion in four bird trials. Birds were housed in individual wire metabolizm cages from 7 to 28 d and offered water and feed ad libitum. Dry matter intake (DMI), live weight gain (LWG) and gain:feed were determined weekly. A balance collection was carried out from 14 to 21 d for determination of apparent metabolizable energy (AME), ME:gain, DM retention, oil and NDF digestibility. At 28 d the birds were sacrificed, the contents of the jejunum removed for determination of in vivo viscosity and the contents of the ileum removed for determination of ileal DM, starch and protein digestibility. The wheat samples used in the study had wide-ranging chemical and physical parameters, leading to bird DMI, LWG, gain:feed, ME:GE, AME content and ileal starch and protein digestibility being significantly (p<0.05) affected by wheat sample. A high level of N fertilizer application to the English and NI wheat samples tended to benefit bird performance, with increases of up to 3.4, 7.2 and 3.8% in DMI, LWG and gain:feed, respectively. Fungicide application also appeared to have a positive effect on bird performance, with fungicide treated (+F) wheat increasing bird DMI, LWG and gain:feed by 6.6, 9.3 and 2.7%, over the non-fungicide treated (-F) wheats. An increase (p<0.1) of 9.3% in gain:feed was also observed at the low seed rate of 40 compared to 640 seeds/$m^2$. It was concluded that the type of wheat sample and environmental growing conditions significantly affects bird performance when fed wheat-based diets.

• 한국미생물·생명공학회지
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• 제41권1호
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• pp.52-59
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• 2013

The nutritional requirements for the maximum production of lipopeptides by Bacillus subtilis N7 (B. subtilis N7) were investigated and optimized using response surface methodology (RSM) under shake flask fermentation. A one-factor-at-a-time experimental setup was used to screen carbon and nitrogen sources. A Plackett-Burman design (PBD) was employed to screen the most critical variables for lipopeptides production amongst ten nutritional elements. The central composite experimental design (CCD) was finally adopted to elucidate the composition of the fermentation medium. Statistical analyses (analysis of variance, ANOVA) of the results showed that KCl, $MnSO_4$ and $FeSO_4{\cdot}6H_2O$ were important components and that their interactions were strong. Lipopeptide production was predicted to reach 709.87 mg/L after a 60 h incubation using an optimum fermentation medium composed of glucose 7.5 g/L, peanut oil 1.25 g/L, $MgSO_4$ 0.37 g/L, $KH_2PO_4$ 0.75 g/L, monosodium glutamate 6.75 g/L, yeast extract and $NH_4Cl$ (5:3 w/w) 10 g/L, KCl 0.16 g/L, $FeSO_4{\cdot}6H_2O$ 0.24 mg/L, $MnSO_4$ 0.76 mg/L, and an initial pH of 7.0. Lipopeptide production ($706.57{\pm}3.70$ mg/L) in the optimized medium confirmed the validity of the predicted model.

• 한국축산식품학회지
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• 제18권4호
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• pp.339-347
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• 1998

The effects of conjugated linoleic acid (CLA) in egg yolk on fatty acid composition and lipid oxidation during chilled storage (4$^{\circ}C$) were investigated. CLA was synthesized according to the method of alkali isomerization using safflower seed oil (SSO). A total of 250 hens (200 days of age) were fed control diet (commercial formula feed for han) or CLA-supplemented diet (1%, 2.5% and 5% CLA) or 5% SSO supplemented diet for 6 weeks, and eggs were collected for analysis of CLA, fatty acid compositons and lipid oxidation. Eggs from CLA-supplemented diets groups showed significantly (p＜0.05) higher CLA content compared to those of control group. The contents of linoleic, palmitic, and myristic acid were increased as well as CLA content by feeding a CLA-supplemented diet. However, the contents of oleic and arachidonic acids in egg yolks were decreased by dietary CLA supplementation. The pH of egg yolk increased by the levels of CLA during storage. The contents of CLA were not significantly (p＜0.05) changed during chilled storage for 28 days, whereas TBARS were significantly (p＜0.05) increased. It is suggested that lipid oxidation of egg yolk might be affected by the levels of CLA in egg yolk due to changes in fatty acid compositions.

• 대한한의학회지
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• 제18권1호
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• pp.187-197
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• 1997

Major separation for the active ingredients and characterization of chemical properties in conjunction with screening test on animal were performed in order to analyze and standardize Ligustici Rhizoma or Angelicae Tenuissimae Radix as an important oriental herbal medicine for antiphlogistic or an important oriental herbal medicine for antiphlogistic or an anodyne. Furthermore the structure, composition and contents of ingredients for essential oil in Angelicae Tenuissimae Radix(Suckpo, Korea) were determined by means of Ge/MS followed by screening test on Z-ligustilide(82%) known as major ingredient as well as butylidenephthalide collected by HPLC with normal phase semiprep-column. The total active ingredient in Ligustici Rhizoma from China or Angelicae Tenuissimae harvested at Choonyang(Kyungnam, Korea), Jungsun(Kangwon, Korea), Suckpo(Kyungnam Korea), Youngchun(Kyungnam, Korea) have been determined showing higher abundant for three times on the product in Korea compared to that in China. In addition, the major component in Ahgelicae Tebyussunae Radux extract was found to be Z-ligustilide(70-80%) which is very different from that in Ligustici Rhizoma senkyunolide(39%) as major species. For screening test of Ligustici Rhizoma or Angelicae Tenuissimae Radix extracts toward the target animal, the efficiency has been shown the similarity on both extracts. Taking into account the level of ingredient, the total efficiency may be three times higher on Angelicae Tenuissimae Radix in Korea compared to Ligustici Rhizoma in China. As a result of present study, it is preferable to distinguish between Ligustici Rhizoma and Angelicae Tenuissimae Radix for better usage of oriental herbal medicine because of very different composition and abundant in spite of their similar screening effect.