• Journal of Microbiology and Biotechnology
• /
• v.17 no.8
• /
• pp.1330-1337
• /
• 2007

To better understand the gene expression of the cold-adapted polar diatom, we conducted a survey of the Chaetoceros neogracile transcriptome by cDNA sequencing and expression of interested cDNAs from the Antarctic diatom. A non-normalized cDNA library was constructed from the C. neogracile, and a total of 2,500 cDNAs were sequenced to generate 1,881 high-quality expressed sequence tags (ESTs) (accession numbers EL620615-EL622495). Based on their clustering, we identified 154 unique clusters comprising 342 ESTs. The remaining 1,540 ESTs did not cluster. The number of unique genes identified in the data set is thus estimated to be 1,694. Taking advantage of various tools and databases, putative functions were assigned to 939 (55.4%) of these genes. Of the remaining 540 (31.9%) unknown sequences, 215 (12.7%) appeared to be C. neogracile-specific since they lacked any significant sequence similarity to any sequence available in the public databases. C. neogracile consisted of a relatively high percentage of genes involved in metabolism, genetic information processing, cellular processes, defense or stress resistance, photosynthesis, structure, and signal transduction. From the ESTs, the expression of these putative C. neogracile genes was investigated: fucoxanthin chlorophyll (chl) a,c-binding protein (FCP), ascorbate peroxidase (ASP), and heat-shock protein 90 (HSP90). The abundance of ASP and HSP90 changed substantially in response to different culture conditions, indicating the possible regulation of these genes in C. neogracile.

• Animal cells and systems
• /
• v.5 no.4
• /
• pp.333-339
• /
• 2001

Common carp (Cyprinus carpio) and its aquaculture breed Israeli carp samples were obtained from two separate aquaculture facilities under the similar raising conditions during two years in the Kunsan National University, Korea. Genomic DNA was isolated from the common carp and Israeli carp for identification of genetic characteristics and genomic polymorphisms by polymerase chain reaction amplification of DNA using arbitrary primers. The arbitrary primer No.21 (ACTTCGCCAC) yielded the highest number of fragments with the average of 15.0 among the primers used in Israeli carp. A tota1 of 294 polymorphic products in common carp and 336 in Israeli carp were observed by random primers. The average number of polymorphic products generated by random RAPD primer No. 2 (GTAGAC-CCGT) showed 8.0 in Israeli carp. On average, each random RAPD primer produced 5.4 amplified polymorphic products in common carp and 6.2 in Israeli carp. An average genetic similarity (BS value) was 0.44$\pm$0.05 within the common carp and 0.32$\pm$0.04 within the Israeli carp. The degree of similarity frequency (BS) between two carps was 0.67 as generated by the primer No. 19 (GACGGATCAG). The average level of bandsharing was 0.57$\pm$0.03 between the two carps. Accordingly, the two carp populations were genetically a little distant. The electrophoretic analysis of PCR-RAPD products showed middle levels of variation between the two carp populations. This result implies that the genetic diversity among intra-population may be higher when compared with that between the two carps. The RAPD polymorphism generated by these random primers might be used as a genetic marker for populations or lines identification in important aquacultural carp.

• Molecules and Cells
• /
• v.20 no.1
• /
• pp.142-150
• /
• 2005

Members of the multifunctional Cyp family have been isolated from a wide range of organisms. However, few functional studies have been performed on the role of these proteins as chaperones in red alga. For studying the function of cDNA GjCyp-1 isolated from the red alga (Griffithsia japonica), we expressed and purified a recombinant GjCyp-1 containing a hexahistidine tag at the amino-terminus in Escherichia coli. An expressed fusion protein, $H_6GjCyp-1$ maintained the stability of E. coli proteins up to $50^{\circ}C$. For a functional bioassay for recombinant $H_6GjCyp-1$, the viability of E. coli cells overexpressing $H_6GjCyp-1$ was compared with that of cells not expressing $H_6GjCyp-1$ at $50^{\circ}C$. After high temperature treatment for 1 h, E. coli overexpressing $H_6GjCyp-1$ survived about three times longer than E. coli lacking $H_6GjCyp-1$. Measurement of the light scattering of luciferase (luc) showed that GjCyp-1 prevents the aggregation of luc during mild heat stress and that the thermoprotective activity of GjCyp-1 is blocked by cyclosporin A (CsA), an inhibitor of Cyps. Furthermore, the Cyp-CsA complex inhibited the growth of E. coli under normal conditions. The results of the GjCyp-1 bioassays as well as in vitro studies strongly suggest that Cyp confers thermotolerance to E. coli.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.38C no.3
• /
• pp.271-277
• /
• 2013

A novel approach to solve signal variation due to ship rocking in maritime wireless communication is introduced. We assume a ship-to-shore based communication scenario, where the transmitter is on shore and the receiver on the ship. Due to the ocean conditions, such as the presence of waves and wind etc. the ship is not stable and constantly experiences some form of rocking motion. This rocking motion causes the antenna on the ship to sway, creating instability in the signal reception. We envisage that the signal is offset at the receiver incurring high Bit Error Rate. This paper is to investigate and counter this problem by using Multiple-input Multiple-output (MIMO) technique. We propose to implement beamforming technique with multiple transmit antennas. The implementation of this proposed method crafts a robust maritime communication network.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.37 no.3
• /
• pp.293-300
• /
• 2013

This study used a water tank and wave maker to generate conditions similar to the real ocean environment. Given that the waves were properly generated in the water tank, a precise analysis indicated the quantitative value of the wave force acting on a body. A high-speed camera and wave-level gauge were used to measure the temporal wave motion and period. A series of artificial water waves were successfully generated using three different wave periods and amplitudes. Each of the waves captured by a high-speed camera was sinusoidal and did not maintain its shape properly without a wave absorber, but it was substantially improved and well shaped when the wave absorber was installed.

• Journal of Navigation and Port Research
• /
• v.37 no.1
• /
• pp.1-7
• /
• 2013

In this paper the developed prediction technique of wave-making resistance performance for a ship attached with a vertical blade had been verified. Numerical analysis program as a prediction technique had been developed using the Rankine source panel method and the vortex lattice method(VLM). The nonlinearity of the free surface conditions was fully taken into account using the iterative method and the trim and the sinkage of the ship were also considered in the numerical analysis program. Panel cutting method was applied to get hull surface panels. Numerical computations were carried out for a 4000TEU container carrier and the vertical blade was attached 6 different locations astern. To investigate the validity of the numerical analysis program the commercial viscous flow field analysis program FLUENT was used to obtain the viscous flow field around the ship and the model test was performed. The model test results were compared with the numerical analysis results.

• Ocean Systems Engineering
• /
• v.2 no.2
• /
• pp.99-113
• /
• 2012

Steel Catenary Risers (SCR) are the simplest and often the most economic solution compared to other riser types such as flexible pipe, riser towers, top tensioned risers, etc. The top of a SCR is connected to the host platform riser porch. The other end of the SCR connects to flowlines from subsea wells. The riser touchdown point (TDP), which is the location along the riser where contact with the sea floor first occurs, exhibits complex behaviors and often results in compression and fatigue related issues. Heave dynamic responses of semisubmersibles in extreme and operating sea states are crucial for feasibility of SCR application. Recent full field measurement results of a deep draft semisubmersible in Hurricane Gustav displayed the considerable discrepancies in heave responses characteristics between the measured and the simulated results. The adequacy and accuracy of the simulated results from recognized commercial software should be examined. This finding raised the awareness of shortcomings of current commercial software and potential risk in mega investment loss and environmental pollutions due to SCR failures. One main objective of this paper is to attempt to assess the importance and necessity of accounting for viscous effects during design and analysis by employing indicator of viscous parameter. Since viscous effects increase with nearly third power of significant wave height, thus newly increased metocean criteria per API in central Gulf of Mexico (GoM) and even more severe environmental conditions in Western Australia (WA) call for fundamental enhancements of the existing analysis tools to ensure reliable and robust design. Furthermore, another aim of this paper is to address the impacts of metocean criteria and design philosophy on semisubmersible hull sizing in WA and GoM.

• Asian Journal of Atmospheric Environment
• /
• v.5 no.4
• /
• pp.263-277
• /
• 2011

Global atmospheric $CO_2$ distributions were simulated with a chemical transport model (GEOS-Chem) and compared with space-borne observations of $CO_2$ column density by GOSAT from April 2009 to January 2010. The GEOS-Chem model simulated 3-D global atmospheric $CO_2$ at $2^{\circ}{\times}2.5^{\circ}$ horizontal resolution using global $CO_2$ surface sources/sinks as well as 3-D emissions from aviation and the atmospheric oxidation of other carbon species. The seasonal cycle and spatial distribution of GEOS-Chem $CO_2$ columns were generally comparable with GOSAT columns over each continent with a systematic positive bias of ~1.0%. Data from the World Data Center for Greenhouse Gases (WDCGG) from twelve ground stations spanning $90^{\circ}S-82^{\circ}N$ were also compared with the modeled data for the period of 2004-2009 inclusive. The ground-based data show high correlations with the GEOS-Chem simulation ($0.66{\leq}R^2{\leq}0.99$) but the model data have a negative bias of ~1.0%, which is primarily due to the model initial conditions. Together these two comparisons can be used to infer that GOSAT $CO_2$ retrievals underestimate $CO_2$ column concentration by ~2.0%, as demonstrated in recent validation work using other methods. We further estimated individual source/sink contributions to the global atmospheric $CO_2$ budget and trends through 7 tagged $CO_2$ tracers (fossil fuels, ocean exchanges, biomass burning, biofuel burning, net terrestrial exchange, shipping, aviation, and CO oxidation) over 2004-2009. The global $CO_2$ trend over this period (2.1 ppmv/year) has been mainly driven by fossil fuel combustion and cement production (3.2 ppmv/year), reinforcing the fact that rigorous $CO_2$ reductions from human activities are necessary in order to stabilize atmospheric $CO_2$ levels.

• Journal of Ecology and Environment
• /
• v.35 no.1
• /
• pp.15-25
• /
• 2012

Time series of ocean climate indices and catch records were used to identify the alternation patterns of pelagic fish populations in relation to climate regime shifts. During 1910-2008, an orderly alternation of dominant pelagic fish groups was observed in the Tsushima Warm Current (TWC; Yellow Sea-East China Sea-East Sea/Japan Sea) and Kuroshio-Oyashio Current (KOC; Northwestern Pacific) regions. After the collapse of herring fishery in the late 1920s, the sardine (A group) dominated in the 1930s, 3 other species (C group; Pacific saury, jack mackerel, and anchovy) dominated in the 1950s-1960s, chub mackerel (B group) dominated in the 1970s, and then sardine (A group) dominated again during cool regime in the 1980s. As sardine biomass decreased in association with the climate regime shift that occurred in the late 1980s, catches of C group immediately increased after the regime shift and remained at high levels during warm regime in the 1990s. Alternations of dominant fish groups occurred 6 times between 1910 and 2008. The dominant period of the 7 species lasted for 10-20 years. The catch of Pacific sardine in the TWC and KOC regions showed a negative correlation with the catch of the other 5 species (Pacific herring, anchovy, jack mackerel, Pacific saury, and common squid), suggesting that the abundance of the 5 species is strongly affected by the abundance of Pacific sardine in relation to the climate regime shifts. The total catch level of the 7 species in the KOC region was generally higher than that in the TWC region before 1991 but was lower after 1992, suggesting that the fish populations in the Pacific side are shifted to the TWC region by zonal oscillation of the oceanic conditions in relation to the climate regime shift in the late 1980s.

• Corrosion Science and Technology
• /
• v.13 no.5
• /
• pp.195-203
• /
• 2014

Modern industry has a wide variety of application areas such as ocean industry, construction and automobile industry. With the current circumstances, the need for anti-corrosion technology that can be used on materials to withstand in harsh environments, is increasing. In this study, we have sought to develop a metal coating technology with zinc and aluminum powders as a potential anti-corrosion material. To make a coating on metal products, a thermal diffusion coating method was used under the conditions of $350^{\circ}C$ for 30 minutes. Optical microscope, Field emission scanning electron microscope (FE-SEM&EDX) and X-ray diffraction analysis were used to analyze a coating layer. As a result, we have confirmed that the generated amount of rust on metal parts coated with thermal diffusion coating method decreased dramatically compared with non-coated metal parts. Furthermore, the anti-corrosion performance was evaluated according to the different ratio of zinc and aluminum. Finally, we confirmed the possibility of application and commercialization of our coating technique on metal parts used in harsh industrial based on the results of these performance.