• Journal of Life Science
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• v.24 no.2
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• pp.186-190
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• 2014

Carbonic anhydrase isozymes (CAs) are widespread zinc-containing metalloenzyme family. The enzyme catalyzes the reversible interconversion of $CO_2$ and $HCO_3$. This reaction is the main role of CA enzymes in physiological conditions. CA III, one of the CA isozymes, has been identified in many tissues. It is distinguished from the other isozymes by several characteristics, particularly by a lower specific activity and by its resistance to acetazolamide. However, the physiological function of CA III in fish is unknown. In this study, we examined the detection of CAs in the Shaggy sea raven Hemitripterus villosus, using SDS-PAGE, isoelectric focusing (IEF), and western blot analysis. We detected a significant protein band with molecular weight about 30 kDa from the tissues of H. villosus by SDS-PAGE and western blotting. A specific band of CA III with pI 7.0 was detected by IEF and western blotting in gill and muscle. The immunoreaction of anti-CA III expressed in the gill of H. villosus was much stronger than other tissues. One explanation for this result is that the fish gill is the only organ that is exposed to the external environment and that plays an important role in acid-base relevant ion transfer, the transfer of $H^+$ and/or $HCO{_3}^-$, for the maintenance of systemic pH. This is the first report on the identification of a carbonic anhydrase III-like protein from H. villosus.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.37 no.1
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• pp.45-51
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• 2001

In order to investigate the optimal design weight of sinkers for preventing cage net from deforming in current, the model experiment on 2 types of square cage nets with different S sub(n)/S, the ratio of total area of netting projected to the perpendicular to the water flow S sub(n) to wall area of netting S, and 4 kinds of sinkers was carried out in circulation water channel. The model cage nets were made in 1/10 scale and the total weight in water of 4 sinkers attached to each corner of their bottom frames was 18, 54, 90, and 126g, respectively equivalent to 0.1, 0.3, 0.5, and 0.7 kg per unit area of prototype net. The results obtained can be summarizes as follows; Due to the deformation of each net where it was lifted towards the surface in severe conditions, its volume was reduced. This depended highly on the weight of sinkers placed in the bottom corner of cage nets, even if the variation of S sub(n)/S had a little effect on their deformation in current less than 0.4 m/s. In addition, it was observed that the total weight of sinkers for preventing the net from deforming to the extent of less than 50% inside its initial volume was 31 to 245 kg in the range of 0.3 to 0.6 m/s and the adequate design weight of sinker was approximately 0.5 kg per its unit area.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.36 no.3
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• pp.157-165
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• 2000

A small hydraulic winch system with an automatic tension control unit was designed to improve the work efficiency of coastal small vessels and the dynamic response characteristics of the winch system operated in the open loop condition was investigated. The inlet and the outlet pressures in hydraulic motor, the torque and the rotating speed of winch drum were measured as a function of time, and the behaviour in autotension mode for stepped load changes was analyzed. The results obtained are summarized as follows : 1. The developed winch system for coastal small vessels will result in better fishing with improved efficiency and lower manpower consumption by remote control of winch system. 2. The rotating delay times of winch drum for on/off operations of solenoid valve were 0.09 see at CW mode and 0.04 sec at CCW mode, respectively. After the solenoid valve was controlled, response characteristics were unstable slightly but showed good tracking behaviour over short time. 3. The driving torque of winch system in autotension mode was kept almost constant of 55.9 kgf·m, and 11.1 then the rotating speed of winch drum was kept almost constant of 5.1 rpm in the larger torque than 55.9 kgf·m and 11.1 rpm in the lower torque than that. 4. The 5% settling times in the transient response characteristics of autotension mode under rapid increasing and decreasing conditions of load were 0.12 sec and 0.2 sec, respectively, and then the rotating speeds were 11 rpm and 5.3 rpm, respectively. 5. The tracking behaviour of torque and rotating speed by remote control operation were stable within 0.23 sec at CW mode and 0.37 sec at CCW mode, respectively.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.31 no.4
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• pp.362-371
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• 1995

This study describes with the sinking behaviour for the purse seine. The experiment was carried out using three model purse seines of knotless netting. The nettings were woven in same leg length(10.5mm) of polypropylene 170 denier, polyamide 210 denier and polyester 250 denier two thread ply twine, each of the seines were named PP seine, PA seine and PES seine. Dimension of Purse seine models were 420cm for corkline and 85cm for seine depth, each models rigged 65.8g, 50.6g and 35.8g(weight in water) of lead for a leadline, seine models will have the same weight in water. Experiments carried out in the observation channel in a flume tank under still water conditions. Sinking motion was recorded by the one set of TV-camera for VTR, and reading coordinate carried out by the video digitization system. The results obtained were as follows: 1) Netting bundle for PP seine was existence on the surface of the water, spread out a netting by sinking force of sinkers. 2) Netting bundle for PES seine was existence in the middle of corkline and leadline. 3) Average sinking speed of seine margin was fastest for 10.7cm/s of PP seine, followed by 9.7cm/s of PA and 9.2cm/s of PES seines. 4) In all seines, the force of horizontal movement for the seine setting was values of ranged in disregard.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.4
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• pp.333-340
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• 1990

The authors carried out a visiualizational model test by the hydrogen bubble method to examine the pattern of the fluid flow besides the simple camber type and plane type otter board in circulation water channel. The experimental conditions are velocity of flow 0.05 and 0.1m/sec, angle of attack 0$^{\circ}$~45$^{\circ}$(5$^{\circ}$step). The results obtained are as follows: 1. In the case of the simple camber type otter board located angle of attack 25$^{\circ}$, vortex at the leading edge was geneated at 1/2 of chord length. 2. Size of the vortex generated in the trailing edge was about 2~3 times larger then that of the leading edge. 3. In the case of the simple camber type otter board located angle of attack 30$^{\circ}$, separation of stream-line at leading edge was generated at 1/3 of chord length. 4. Nearest stream-line in the back side of the simple camber type otter board was bent in the direction of otter board when the angle of attack was 25$^{\circ}$ and 30$^{\circ}$, and in the case of plane type otter board was expanded outside of the flow direction. 6. Area separated of the simple camber type otter board at the angle of attack 30$^{\circ}$ was smaller then that of plane type otter board. 7. Flow speed in the back side of the simple camber type otter board was about 1.4 times faster then that in the front side, and in the case of the plane otter board about 1.2 times faster.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.1
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• pp.79-92
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• 2011

In this study, wave pressure of short-period gravity waves and tsunami acting on the upright section of the horizontal-slit type caisson placed on the impermeable or permeable seabed, which is a well-known permeable breakwater with a good wave controlling ability, are investigated via numerical simulations. Further, the permeable seabed was modeled as the porous media with porosity of 0.4. Using the numerical results, the effects of the seabed conditions on the wave pressure on the front wall and inside wall of the chamber have been studied. In the numerical simulations, short-period gravity waves and tsunami(solitary wave or bore) with the same amplitude to the gravity wave are considered. A numerical wave tank is used, which is able to consider a gas-liquid two-phase flow in the same calculation zone. Numerical results show that the wave pressure of the tsunami was 3~5 times higher than the short-period gravity waves acting on the front wall and it was 2~4 times higher than the short-period gravity waves acting on the inner wall.

• Ocean and Polar Research
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• v.28 no.2
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• pp.129-144
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• 2006

Small-scale effects of sand mining on macrobenthic communities have been reported previously. However, little information is available as to how dredging affects the macrobenthic community structure. The objective of this study was to determine the impacts of large-scale exhibition dredging on the macrobenthic community of Gyeonggi Bay, Korea, where sand mining has continued for 20 years. Prior to dredging, the macrobenthic species composition was similar to that of areas near the dredging site, with several common dominant species found before dredging. After dredging, the number of species, density, and diversity (H') in the experimental area (sites 0 and 1) decreased significantly, but no difference was observed at other sites. Multivariate analysis (multidimensional scaling) revealed significant differences in community structure before and after dredging. The amphipod Urothoe grimaldii japonica, which was the most dominant species at sites 0,1, and 2, decreased rapidly at sites 0 and 1 after dredging, but no difference was observed at site 2. In addition, the index of multivariate dispersion (IMD) and the relative IMD (r. IMD) were large at sites 0 and 1, suggesting that the effects of dredging were direct at site 0 and 1, but indirect at site 2. The macrobenthic communities at sites 3 and 4 were not affected by dredging, but they were affected by physical conditions and biological interactions. We suggest that benthic biotope indices such as the IMD and r.IMD may constitute a valid tool for assessing the effects of dredging on the macrobenthic community; long-term monitoring is required to verify this.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.6
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• pp.675-681
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• 1994

[ $F_0$ ]-values of the canned tuna in cottonseed oil (CTCO) were investigated under different sterilizing conditions to optimize the energy consumption and microbiological safety. The $F_0$-values were measured using a microcomputer based technique. The exact cold point was not the volumetric center of the cans, and it was located in the center of meat mass in can which had ca. $6\%$ of head space. Location of the test cans in retort showed no remarkable influence on the $F_0$-values when the cans were jumble loaded. The process time before sterilization should be shortened as much as possible to prevent the contamination of microorganisms. Thermophilic spore forming bacteria found from raw and precooked tuna were Bacillus subtilis, Bacillus cereus and Bacillus pasteurii, and the most heat resistant was Bacillus subtilis. The rational $F_0$-value for the CTCO obtained from the preservation test was regarded as 6min.

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2002.05a
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• pp.23-28
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• 2002

This paper describes the machining characteristics of the $MoSi_2$ based composites by electric discharge drilling with various tubular electrodes, besides, Hardness characteristics and microstructures of $Nb/MoSi_2$ laminate composites were evaluated from the variation of fabricating conditions such as preparation temperature, applied pressure and pressure holding time. $MoSi_2$ -based composites has been developed in new materials for jet engine of supersonic-speed airplanes and gas turbine for high- temperature generator. Achieving this objective may require new hard materials with high strength and high temperature-resistance. However, With the exception of grinding, traditional machining methods are not applicable to these new materials. Electric discharge machining (EDM) is a thermal process that utilizes a spark discharge to melt a conductive material, the tool electrode being almost non-unloaded, because there is no direct contact between the tool electrode and the workpiece. By combining a nonconducting ceramics with more conducting ceramic it was possible to raise the electrical conductivity. From experimental results, it was found that the lamination from Nb sheet and $MoSi_2$ powder was an excellent strategy to improve hardness characteristics of monolithic $MoSi_2$. However, interfacial reaction products like (Nb, Mo)$SiO_2$ and $Nb_2Si_3$ formed at the interface of $Nb/MoSi_2$ and increased with fabricating temperature. $MoSi_2$ composites which a hole drilling was not possible by the conventional machining process, enhanced the capacity of ED-drilling by adding $NbSi_2$ relative to that of SiC or $ZrO_2$ reinforcements.

• Journal of Navigation and Port Research
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• v.39 no.3
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• pp.233-239
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• 2015

Recently, Due to variable marine accident occurred, problems relating to marine accidents have been raised. Of these accidents, designated anchorage of Korea coastal port, spcially open-sea anchorage, occurred dragging anchor and accidents are likely, due to failure to obtain a valid ship's holding power so that it is the situation unsatisfactory that ensuring the safety of the anchored ship and anchorage systematic safety management stadars for the efficent operation of the anchorage. also in case of open-sea anchorage vulnerable to external force of certain conditions due to geographical location and topographical characteristics of the port, accidents are likely dragging anchor by typoon or suddenly strong wind and secondary accidents are concerned. This paper shows standard of limit external force to ensure the safety anchorage each of ship's size through review marine accidents on Pohang port, also it is determined to be used as a basic reference for anchoring safety and efficient anchorage management.