• Title/Summary/Keyword: objective of lab activity

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Analysis of Mental Health Level and Life Satisfaction According to the Amount of Physical Activity and Muscular Strength Level

  • Jaehyun Yoo
    • Physical Therapy Rehabilitation Science
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    • v.11 no.4
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    • pp.598-603
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    • 2022
  • Objective: About one-third of college students are exposed to stress to the point that their academic performance is negatively affected. To provide useful information for managing the mental health of young people by analyzing the perceived stress level, depression level, and life satisfaction according to the amount of physical activity and the level of muscular strength in male college students. Design: A cross-sectional study. Methods: Among 304 students who participated in the health exercise class at Sahmyook University in Seoul, the amount of physical activity and the level of muscular strength were divided into 3 quartiles, respectively, and the perceived stress level, depression level, and life satisfaction were analyzed through one-way ANOVA. The physical activity level and muscle strength level of the subjects were ranked, divided into 3 groups, and the difference in variables according to the group was verified. Results: There was no difference in perceived stress level, depression level, and life satisfaction according to the amount of physical activity, but statistically significant differences were shown in perceived stress scale(p=0.008) and life satisfaction(p=0.030) according to muscular strength. Conclusions: Policies to increase physical activity are important to improve and manage the mental health of young people, but it is judged to be more meaningful to provide an environment that can improve muscular strength.

Isolation, Characterization, and Comparative Genomics of the Novel Potential Probiotics from Canine Feces

  • Ngamlak Foongsawat;Sirinthorn Sunthornthummas;Kwannan Nantavisai;Komwit Surachat;Achariya Rangsiruji;Siriruk Sarawaneeyaruk;Kedvadee Insian;Sirapan Sukontasing;Nuttika Suwannasai;Onanong Pringsulaka
    • Food Science of Animal Resources
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    • v.43 no.4
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    • pp.685-702
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    • 2023
  • Lactic acid bacteria (LAB) are commonly used as probiotics; however, not all LAB strains have the same beneficial effects. To successfully use LAB as probiotics in canines, LAB species should originate from the canine intestinal tract as they display host specificity. The objective of this study was to investigate the phenotypic and genomic traits of potential probiotic LAB isolated from canine fecal samples. Twenty LAB samples were evaluated for their potential probiotic characteristics including resistance to low pH, bile salts, hydrophobicity, auto-aggregation, co-aggregation, adhesion to epithelia or mucosa, and production of inhibitory compounds. Additionally, we evaluated their safety and other beneficial effects on canine health, such as DPPH free radical scavenging, and β-galactosidase. Four strains demonstrated potential probiotic characteristics and were selected: Enterococcus hirae Pom4, Limosilactobacillus fermentum Pom5, Pediococcus pentosaceus Chi8, and Ligilactobacillus animalis FB2. Safety evaluations showed that all strains lacked hemolytic activity, could not produce biogenic amines, and did not carry any pathogenic genes. In addition, L. fermentum Pom5 and P. pentosaceus Chi8 displayed susceptibility to all antibiotics and concordant with the absence of antibiotic resistance genes. Based on their phenotypic and genomic characteristics, L. fermentum Pom5 and P. pentosaceus Chi8 were identified as potential probiotic candidates for canines.

Physicochemical components and antioxidant activity of Sparassis crispa mixture fermented by lactic acid bacteria (유산균 발효 꽃송이버섯 혼합물의 이화학적 성분과 항산화 활성)

  • Lee, Jae-Joon;Son, Hye-Young;Choi, Young-Min;Cho, Jae-Han;Min, Jung-Kee;Oh, Hee-Kyung
    • Food Science and Preservation
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    • v.23 no.3
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    • pp.361-368
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    • 2016
  • The objective of this study was to investigate the nutritional composition and antioxidant activity of a mixture of rice bran and bodies of Sparassis crispa fermented with lactic acid bacteria (LAB). LAB-fermented S. crispa mixture had higher water, crude lipid and crude ash content than that of S. crispa. Insoluble dietary fiber contents of the dried powder of S. crispa and LAB-fermented S. crispa mixture were 46.13% and 33.46%, respectively. ${\beta}$-glucan was higher in dried S. crispa (38.03%) than in LAB-fermented S. crispa mixture (5.44%). Dried S. crispa contained mainly fructose and glucose instead of containing sucrose in LAB-fermented S. crispa mixture. No significant differences in the total polyphenol contents were found in between dried S. crispa and LAB-fermented S. crispa mixture. Total flavonoid content was significantly higher in LAB-fermented S. crispa mixture than in dried S. crispa. No significant differences were found in the DPPH radical scavenging activity and in the antioxidant index between dried S. crispa and LAB-fermented S. crispa mixture. Finally, ABTS radical scavenging activity of LAB-fermented S. crispa mixture was significantly higher than that of dried S. crispa. These results may provide the basic data for future studies for a better understanding of the biological activities of LAB-fermented S. crispa mixture.

The Effect of Peer Review Activities on Qualitative Changes in Lab Reports (동료 검토 활동이 실험보고서의 질적 변화에 미치는 영향)

  • Park, Sung-Hye;Kang, Seong-Joo;Jang, Eun-Kyung
    • Journal of The Korean Association For Science Education
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    • v.30 no.8
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    • pp.988-1001
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    • 2010
  • The purpose of this research was to investigate how the peer review activity of lab report in the problem-solving experiment effected on the description ability and the quality improvement. The students who were taking the general chemistry experiment course were the subjects for this study. They finished lab reports and received peer review from their peers more than two times. The students who got feedback answered on peer review, revised their reports, and subsequently, completed final reports. The result showed that peer review affected the qualitative improvement of the lab report, specially in the process of 'designing experiment' and 'drawing conclusion and evaluation'. Students could organize their thoughts through writing lab reports. During this process, peer review activities provided the opportunity of self-examination and the way for viewing as objective standpoint. Moreover, the activities established communication fields for exchanging mutual opinions and learning.

Feasibility of sodium long chain polyphosphate as a potential growth promoter in broilers

  • Moon, Seung-Gyu;Kothari, Damini;Kim, Woong-Lae;Lee, Woo-Do;Kim, Kyung-Il;Kim, Jong-Il;Kim, Eun-Jib;Kim, Soo-Ki
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1286-1300
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    • 2021
  • The objective of this study was to evaluate in vitro antimicrobial and anti-biofilm activity of sodium long chain polyphosphate (SLCPP) and effect of dietary supplementation of SLCPP on growth performance, organ characteristics, blood metabolites, and intestinal microflora of broilers. Antimicrobial activities of SLCPP were observed against Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica ser. Pullorum, Shigella sonnei, Klebsiella pneumonia, Pseudomonas aeruginosa in agar well diffusion assay. In addition, SLCPP demonstrated good anti-biofilm activity against K. pneumonia and P. aeruginosa. Furthermore, to investigate the dietary effect of SLCPP, a total of 480 1-day-old male Ross 308 broiler chicks were randomly allotted to three dietary treatment groups (4 replicates per group, 40 birds in each replicate): an antibiotic-free corn-soybean meal basal diet (NC); basal diet + enramycin 0.01% (PC); and basal diet + 0.1% SLCPP (SPP). The experiment lasted for 35 days. Results showed that birds fed with SLCPP had higher body weight (BW) and average daily gain (ADG), and lower feed conversion ratio (FCR) during the grower phase (days 7 to 21) (p < 0.05). Except for blood urea nitrogen, all other blood biochemical parameters remained unaffected by the dietary supplementation of SLCPP. Compared to the control group, lengths of the duodenum and ileum in the SPP group were significantly shorter (p < 0.05). Moreover, counts of lactic acid bacteria (LAB), total aerobes, and Streptococcus spp. in jejunum as well as LAB in cecum were increased in the SPP group than in the PC group (p < 0.05). These results suggest that dietary supplementation of SLCPP might promote the growth of broilers in their early growth phase.

Assessment of lactic acid bacteria isolated from the chicken digestive tract for potential use as poultry probiotics

  • Merisa Sirisopapong;Takeshi Shimosato;Supattra Okrathok;Sutisa Khempaka
    • Animal Bioscience
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    • v.36 no.8
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    • pp.1209-1220
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    • 2023
  • Objective: The use of probiotics as an alternative to antibiotics in animal feed has received considerable attention in recent decades. Lactic acid bacteria (LAB) have remarkable functional properties promoting host health and are major microorganisms for probiotic purposes. The aim of this study was to characterize LAB strains of the chicken digestive tract and to determine their functional properties for further use as potential probiotics in poultry. Methods: A total of 2,000 colonies were isolated from the ileum and cecal contents of the chickens based on their phenotypic profiles and followed by a preliminary detection for acid and bile tolerance. The selected 200 LAB isolates with exhibited well-tolerance in acid and bile conditions were then identified by sequencing the 16S rDNA gene, followed by acid and bile tolerance, antimicrobial activity, adhesion to epithelial cells and additional characteristics on the removal of cholesterol. Then, the two probiotic strains (L. ingluviei and L. salivarious) which showed the greatest advantage in vitro testing were selected to assess their efficacy in broiler chickens. Results: It was found that 200 LAB isolates that complied with all measurement criteria belonged to five strains, including L. acidophilus (63 colonies), L. ingluviei (2 colonies), L. reuteri (58 colonies), L. salivarius (72 colonies), and L. saerimneri (5 colonies). We found that the L. ingluviei and L. salivarius can increase the population of LAB and Bifidobacterium spp. while reducing Enterobacteria spp. and Escherichia coli in the cecal content of chickens. Additionally, increased concentrations of valeric acid and short chain fatty acids were also observed. Conclusion: This study indicates that all five Lactobacillus strains isolated from gut contents of chickens are safe and possess probiotic properties, especially L. ingluviei and L. salivarius. Future studies should evaluate the potential for growth improvement in broilers.

Effects of Dietary L-carnitine Supplementation on Growth Performance, Organ Weight, Biochemical Parameters and Ascites Susceptibility in Broilers Reared Under Low-temperature Environment

  • Wang, Y.W.;Ning, D.;Peng, Y.Z.;Guo, Y.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.2
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    • pp.233-240
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    • 2013
  • The objective of this study was to investigate the effects of L-carnitine on growth performance, organ weight, biochemical parameters of blood, heart and liver, and ascites susceptibility of broilers at different ages reared under a low-temperature environment. A total of 420 1-d-old male Ross 308 broilers were randomly assigned to two dietary treatments with fifteen replicates of fourteen broilers each. Treatment diets consisted of L-carnitine supplementation at levels of 0 and 100 mg/kg. At 11-d of age, low temperature stress was used to increase ascites susceptibility. Blood, heart and liver samples were collected at different ages for analysis of boichemical parameters. The results showed that, there was no significant difference in growth performance with L-carnitine supplementation, but the mortality due to ascites was significantly decreased. Dietary L-carnitine supplementation significantly reduced heart index (HI) and ascites heart index (AHI) on d 21, lung index (LUI) on d 35 and liver index (LI) on d 42. The broilers fed diets containing L-carnitine had significantly lower red blood cell counts (RBC), hemoglobin (HGB) concentration and hematocrit (HCT) on d 42. Dietary L-carnitine supplementation significantly reduced malondialdehyde (MDA) content of heart tissue on d 21 and 35, and significantly increased total superoxide dismutase (T-SOD) and Glutathione peroxidase (GSH-Px) activity of the heart on d 21 and 42. L-carnitine supplementation significantly reduced serum triglyceride (TG) content on d 28 and 35 and serum glucose (GLU) on d 35 and 42, and significantly increased serum total protein (TP) and globulin (GLO) content on d 42. L-carnitine supplementation significantly enhanced liver succinodehydrogenase (SDH), malic dehydrogenase (MDH) and $Na^+$-$K^+$-ATPase activity on d 28, and tended to reduce the lactic acid (LD) level of liver on d 35 (p = 0.06). L-carnitine supplementation significantly reduced serum uric acid (UA) content on d 28, 35 and 42. Based on the current results, it can be concluded that dietary L-carnitine supplementation reduced organ index, red blood cell counts and hematocrit, enhanced antioxidative capacity of the heart, enhanced liver enzymes activity involved in tricarboxylic acid cycle, and reduced serum glucose and triglyceride. Therefore, it is suggested that L-carnitine can potentially reduce susceptibility and mortality due to ascites.

Effect of the mixed culture of heterofermentative lactic acid bacteria and acid-tolerant yeast on the shelf-life of sourdough (이상발효유산균과 내산성 효모와의 혼합배양이 사워도우의 저장성에 미치는 영향)

  • Lim, Eun-Seo
    • Korean Journal of Microbiology
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    • v.52 no.4
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    • pp.471-481
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    • 2016
  • The primary objective of this study was to investigate the effects of the bacteriocin-producing heterofermentative lactic acid bacteria (LAB) and acid-resistant yeast isolated from Mukeunji, a Korean ripened kimchi on shelf-life extension and quality improvement of sourdough. According to gene sequence analysis the heterofermentative LAB that showed the antimicrobial activity against bread-spoilage Bacillus strains were identified as Leuconostoc mesenteroides LAS112, Lactobacillus brevis LAS129, and L. mesenteroides subsp. dextranicum LAB137. In addition, the yeasts that were able to grow at acidic pH were identified as Pichia membranifaciens YS05, Pichia fermentans YS19, and Pichia anomala YS26. During sourdough fermentation the levels of acetic acid and bacteriocin produced by L. brevis LAS129 strain were higher than those of L. mesenteroides LAS112 and L. mesenteroides subsp. dextranicum LAS 137 strains, whereas LAS112 strain produced the highest levels of lactic acid. The maximum bacteriocin activity (640 AU/g) against Bacillus subtilis ATCC 35421 was obtained in sourdough fermented by mixed culture of L. brevis LAS129 and P. membranifaciens YS05 or P. anomala YS26. After 24 h of fermentation at $30^{\circ}C$, the viable cell counts of LAS129 ($10^9CFU/g$) in sourdough were higher than those of the YS05 or YS26 ($10^7CFU/g$). Meanwhile, the viable cells of bread-spoilage strain in sourdough fermented with these strains were significantly (P < 0.05) lower than the control group.

AE Characteristics of Fatigue Crack Opening and Closure in Structural Aluminum Alloy (구조용 알루미늄 합금에서의 피로균열 열림 및 닫힘 시 AE 발생특성 연구)

  • Jeong, Jung-Chae;Park, Phi-Lip;Kim, Ki-Bok;Lee, Seung-Seok;Yoon, Dong-Jin
    • Journal of the Korean Society for Nondestructive Testing
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    • v.22 no.2
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    • pp.155-169
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    • 2002
  • The objective of this study was to investigate the effect of crack opening and closure in the AE activities during fatigue test. Laboratory experiment using various materials and test conditions were carried out to identify AE characteristics of fatigue crack propagation. Compact tension specimens of 2024-T4 and 6061-T6 aluminum alloy were prepared for fatigue test. AE activities were analyzed based on the phase of the loading cycle. Generally, most of AE were generated when the crack begins' opening and the crack closes fully, whereas a few in the pull opening of the crack. Also AE activity in the peak loading of cycle was different with each specimen. However, in the same material, AE activity was not affected by the change of cyclic frequency (0.1, 0.2, 1.0Hz). It was found that AE activities during crack opening and closure depend on material properties such as micro-structure, tensile strength and yield strength.

Isolation of the Cholesterol-Assimilating Strain Pediococcus acidilactici LRCC5307 and Production of Low-Cholesterol Butter

  • Kim, Yunsik;Yoon, Seokmin;Shin, Hyejung;Jo, Miyoun;Lee, Sunmin;Kim, Sae-hun
    • Food Science of Animal Resources
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    • v.41 no.2
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    • pp.300-311
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    • 2021
  • The objective of the present study was to evaluate the cholesterol-assimilation ability of lactic acid bacteria (LAB), which were isolated from kimchi, a Korean traditional fermented cabbage. The isolated strain, using modified MRS medium, showed 30.5% cholesterol assimilation activity and was named Pediococcus acidilactici LRCC5307. Types and concentrations of bile were investigated for their effects on increasing the cholesterol assimilation ability of the LRCC5307 strain, a 74.5% decrease in cholesterol was observed when 0.2% bile salts were added. In addition, the manufacture of low-cholesterol butter using LRCC5307 was examined. After fermentation, LRCC5307 with butter showed 8.74 Log CFU/g viable cells, pH 5.43, and a 11% decrease in cholesterol. These results suggest that LRCC5307 could help in the production of healthier butter by decreasing cholesterol and including living LAB.