• 제목/요약/키워드: ob/ob

검색결과 846건 처리시간 0.029초

Ob/Ob 마우스에서 계지복령환(桂枝茯苓丸)과 계지복령환(桂枝茯苓丸) 합강지환(合降脂丸)이 비만 및 지방대사에 미치는 영향 (Effect of Geijibokryung-hwan and Combination of Geijibokryung-hwan and Gangji-hwan on Obesity and Lipid Metabolism in Ob/Ob Mice)

  • 김민애;송정오;이인선
    • 대한한방부인과학회지
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    • 제31권1호
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    • pp.20-42
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    • 2018

  • Objectives: This study was designed to investigate anti-obesity effects the improvement effects of Gyejibongnyeong-hwan and Gyejibongnyeong-hwan-Gangji-hwan (CIPPDF) in a ob/ob mouse model. Methods: Seven-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as normal group and obese ob/ob mice were randomly divided into 4 groups. a normal group given a standard diet, an obese control group given a standard diet with CIPP (300 mg/kg), CIPPDF (1) (300+300 mg/kg), CIPPDF (2) (300+600 mg/kg) respectively. After 10 weeks of treatment, body weight gain, feeding efficiency ratio, blood lipid markers, mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and lipogenesis in in-vivo, were examined. Results: 1. Body weight gain and Feeding efficiency ratio were significantly decreased in CIPPDF (1) compared with control. Fat mass was significantly decreased in CIPPDF (2) in EAT compared with control. 2. Consistent their effects on body weight gain and fat mass, circulating concentrations of LDL-cholesterol were decreased in CIPPDF (1), CIPPDF (2) groups compared with control. 3. MCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the liver, epididymal adipose tissue compared with control. VLCAD mRNA levels of genes was increased in CIPPDF (1), CIPPDF (2) groups in the skeletal muscle compared with control. 4. $PPAR{\gamma}$ mRNA was decreased in CIPPDF (1) in the liver compared with control. SCD1 mRNA was decreased in CIPPDF (1), CIPPDF (2) groups in the epididymal adipose tissue compared with control. Conclusions: In conclusion, These results suggest that CIPPDF not only decrease feeding efficiency ratio, and LDL-cholesterol, but also reduce EAT fat mass contributing to the improvement of ovesity. CIPPDF also were increased in mRNA levels of genes involved in fatty acid ${\beta}-oxidation$ and decreased in mRNA levels of genes involved in lipogenesis.

  • https://doi.org/10.15204/jkobgy.2018.31.1.020 인용 PDF KSCI

역병에 대한 Enterobacter asburiae ObRS-5 처리의 유도저항성 발현 (Induction of systemic resistance against Phytophthora blight by Enterobacter asburiae ObRS-5 with enhancing defense-related genes expression)

  • 김다연;전용희;안재형;안시현;윤영건;박인철;박진우
    • 환경생물
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    • 제38권4호
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    • pp.724-732
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    • 2020

  • 본 연구에서는 기 선발한 Enterobacter asburiae ObRS-5 균주를 1×108 cfu mL-1 농도로 고추에 관주 처리했을 때 Phytophthora capsici에 의한 고추역병을 74.6% 방제하는 효과가 있었다. E. asburiae ObRS-5 균주에 의한 고추역병 방제 메커니즘을 확인하기 위해 고추의 PR1, PR4 및 PR10 유전자를 특이적으로 증폭하는 프라이머를 이용하여 quantitative PCR을 수행하였다. 그 결과 E. asburiae ObRS-5 균주를 처리한 고추에서 대조구와 비교하여 상기 세 가지 유전자의 발현이 모두 높은 수준을 유지하였다. 또한 E. asburiae ObRS-5 균주는 고추의 생육을 억제하지 않으면서 ISR 반응을 유도하는 것으로 나타났다. 이러한 결과를 통하여 P. capsici이 침입할 때 E. asburiae ObRS-5 균주가 매개하는 ISR 메커니즘을 통해 Phytophthora 역병의 제어가 가능한 것으로 사료된다.

  • https://doi.org/10.11626/KJEB.2020.38.4.724 인용 PDF KSCI

한방부인과 진단용 설문지의 신뢰도 연구 (Reliability Study of Oriental OB & GY Questionnaires)

  • 이인선;전란희;배경미;김미진;엄윤경;이용태;지규용;김종원;기규곤
    • 동의생리병리학회지
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    • 제18권3호
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    • pp.701-712
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    • 2004

  • This study investigated reliability of Oriental OB & GY Questionnaires, evaluated the items and correlation relation of differentiation of syndromes of Oriental OB&GY Questionnaires which was used by Dong-Eui OB&GY. We analyzed the results of 553 outpatients's Questionnaires from April 2002 to February 2004. The reliability of Oriental OB&GY Questionnaires above 95% was stagnated blood most, above 90% deficiency of blood, deficiency of Um, heat syndrome, dampness, kidney, liver, heart, spleen, with the exception of phlegm all that was above 80%. The frequency diagnosed of items of differentiation of syndromes dampness, hear, stagnation of Ki was most, the average of item of differentiation of syndromes stagnation of Ki, dampness, deficiency of Ki was most. Correlation coefficient of deficiency of Ki, deficiency of blood, deficiency of Um, cold syndrome, heat, stagnated blood, heart, spleen, kidney, dryness, stagnation of Ki, liver with more than pure question was above 0.8, phlegm was under 0.5. The frequency diagnosed of items of the differentiation of syndromes was not which is related to repeated question and physiology and pathology of oriental medicine.

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