• Proceedings of the PSK Conference
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• 2003.04a
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• pp.290.3-291
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• 2003

To increase detection sensitivity for multi-DDT residues (o,p-/p,p-DDT, o,p-/p,p-DDE, o,o-/o,p-DDD) analysis, a highly selective sample clean-up method was introduced prior to GC/MS analysis using immunoaffinity column. The immunoaffinity matrix was prepared by coupling IgG fraction of DDT antiserum to cyanogens bromide activated Sepharose 4B. Three DDT antisera (DDA-1, DDHP-2, DDCP-3) were test for affinity column ligand that obtained by imunizing respective DDT immunogen to rabbits, and IgG was purified using protein A affinity purification. (omitted)

• Journal of Environmental Science International
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• v.14 no.2
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• pp.193-199
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• 2005

To obtain the residual organochlorine pesticides in the ginseng, the methods of multi-analysis for BHC's isomer, DDT's isomer and other organochlorine pesticides by GC-ECD are surveyed. The relative retention time for $\alpha-BHC,\;\beta-BHC,\;\delta-BHC\;and\;\gamma-BHC$ is 1.000, 1.025, 1.034 and 1.056, respectively. The relative retention time for o,p-DDE, p,p-DDE, o,o-DDD, o,p-DDT, o,p-DDD, and p,p-DDT is 1.199, 1.230, 1.242, 1.286, 1.329 and 1.333, respectively. The BHC isomers, DDT's isomer and other organochlorine pesticides are separated with multianalysis condition. The qualified defection concentration for $\alpha-BHC$, Quintozene, Aldrin, Captan, $\alpha-Endosulfan$, and Dieldrin is 0.95ng/g, 0.27ng/g, 1.04ng/g, 0.63ng/g, 0.55ng/g and 0.62ng/g, respectively. The qualified defection concentration for Fenhexamid, Endrin, $\beta-Endosulfan$, o,p-DDT, Endosulfan-sulfate is 5.71ng/g, 0.61ng/g, 0.48ng/g, 0.44ng/g and 0.51ng/g, respectively. BHC, Aldrin, Dieldrin, Endrin and DDT, which were Korea Food & Drug Administration advisory pesticides, are not detected in soil environment. Also it's residual organochlorine pesticides are not polluted in the ginseng on Sangju Korea.

• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.123-128
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• 2008

The MRLs (maximum residue limits) of DDT (DDD and DDE) in fresh ginseng, dried ginseng, and steamed red ginseng are set as low as 0.01 mg/kg, 0.05 mg/kg, and 0.05 mg/kg, respectively. Therefore, this study was undertaken to develop a simple and highly sensitive analysis method, as well as to reduce interfering ginseng matrix peaks, for the determination of DDT isomers (o,p'-DDE, p,p'-DDE, o,p'-DDD, p,p'-DDD, o,p'-DDT, and p,p'-DDT) in fresh ginseng, dried ginseng, and steamed red ginseng at the 0.01 mg/kg level. The method used acetonitrile extraction according to simultaneous analysis, followed by normal-phase Florisil solid-phase extraction column clean-up. The purification method entailed the following steps: (1) dissolve the concentrated sample extract in 7 mL hexane; (2) add 3 mL of $H_2SO_4$; (3) vigorously shake on avortex mixer; (4) cetrifuge at 2000 rpm for 5 min; (5) transfer 3.5 mL of the supernatant to the Florisil-SPE (500 mg/6 mL);and (6) elute the SPE column with 1.5 mL of hexane and 10 mL of ether/hexane (6:94). The determination of DDT isomers was carried out by a gas chromatography-electron capture detector (GC-${\mu}$ECD). The hexane and ether/hexane (6:94) eluate significantly removed chromatographic interferences, and the addition of 30% $H_2SO_4$ to the acetonitrile extract effectively reduced many interfering ginseng matrix peaks, to allow for the determination of the DDT isomers at the 0.01 mg/kg level. The recoveries of the 6 fortified (most at 0.01 mg/kg) DDT isomers from fresh ginseng, dried ginseng, and steamed red ginseng ranged from 87.9 to 99.6%. The MDLs (method detection limits) ranged from 0.003 to 0.009 mg/kg. Finally, the application of this method for the determination of DDT isomers is sensitive, rapid, simple, and inexpensive.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.05a
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• pp.75-75
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• 2002

• Korean Journal of Environmental Agriculture
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• v.5 no.2
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• pp.113-118
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• 1986

After selecting four places in the main shellfish habitat, 118 items of the sample were collected in the mud flat from August to October in 1983, in order to analyze organochlorine pesticide residues by a gas chromatograph equipped with an electron capture detector. The results obtained were summarized as follows; ${\alpha}-BHC,\;{\gamma}-BHC\;&\;PCNB,\;heptachlor,\;{\gamma}-endosulfan,\;{\beta}-endosulfan,$ p, p'-DDE, dieldrin, o, p'-DDT and p, p'-DDT were detected in the range from 2 to 98 percent and their levels of mean residues ranged from trace to 0.041ppm, in the order of ${\gamma}-BHC\;&\;PCNB,\;{\alpha}-BHC,\;heptachlor{\approx}{\alpha}-endosulfan{\approx}p, p`-DDE{\approx}dieldrin{\approx}{\beta}-endosulfan{\approx}$o, p'-DDT, and p, p'-DDT. The mean of total residues in regions was Kangjin(0.058ppm), Kwangyang(0.080ppm), Yoch'on (0.016ppm), and Yonggwang(0.75ppm). ${\gamma}-BHC\;&\;PCNB$ peak were separated by column packed with DC-200. PCNB was identified by making a PCA(pentachloroaniline), the reduced derivative of PCNB, and this confirmed that PCNB residues were detected in the soil sample.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11b
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• pp.381-383
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• 2003

유기염소계농약류 25종을 분석한 결과 HCHs(4종, endosulfan(2종), octachlorostyrene은 모든 시료에서 검출되지 않았으며 trans-nonachlor, heptachlor는 각각 한 지점의 붕어에서 검출되었고 나머지는 검출되지 않았다. Chlordane의 경우 두 이성질체가 한 지점의 붕어에서 검출되었고, DDT는 6개 이성질체 중 p,p’-DDT와 p,p’-DDD가 한 지점의 붕어에서 검출되었으며 o,p’-DDT는 한 지점의 황소개구리에서 검출되었다. 그 외의 이성질체는 모든 시료에서 검출되지 않았다.

• Korean Journal of Environmental Agriculture
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• v.6 no.2
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• pp.12-21
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• 1987

Residues of some organo-chlorine pesticides on 108 solis collected from 5 sites of Chungnam avea during March 20-April 6, 1986 were investigated. 12 different kinds of Chemical components were detected showing the highest detection frequency with P.P'-DDE(67.6%) and the lowest with Heptachlor(1.8%). 4 different chemicals, ${\alpha}-BHC$, ${\gamma}-chlordane$, P.P'-DDD and P.P'-DDD and P.P'-DDE showed their average contents in the soils of 0.001 ppm and 0.005ppm for ${\beta}-BHC$, respectively. On the other hand, ${\alpha}-BHC$, ${\gamma}-chlordane$, P.P'-DDT. O.P'-DDT and P.P'-DDD showed 0.002ppm for their average contents of residues, respectively.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2001.12a
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• pp.127-127
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• 2001

• Proceedings of the PSK Conference
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• 2002.04a
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• pp.135.2-136
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• 2002

• Korean Journal of Environmental Biology
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• v.21 no.2
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• pp.170-176
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• 2003

The effect of xenobiotics on cytochrome P450 (CYP) and 7-ethoxyresorufin-O-deethylase (EROD) in hepatopancreatic microsome of surf clam, Coelomactra anti-quata, were investigated. The microsome isolated from the digestive gland of the surf clam, collected from the east coast of Korea, was in vitro exposed to p, p -DDT (0.1,0.4 and 1.0 mM) for 30 min and 2,3,7,8-TCDD (0.01, 0.04 and 0.1 ppb) and PCB-153 (0.01, 0.04 and 0.1 ppb) for 7 hr. In the case of DDT exposure, the CYP content and EROD activity of 1.0 mM exposure group increased up to about 117% and 120% of the DMSO solvent control group after 10 min. exposure, respectively. After 2 hr exposure of TCDD, the CYP content and EROD activity were also induced to the range of 103∼110% and 121∼139%, respectively. The PCB-153 exposure group showed 107∼115% of CYP content and 129∼140% of EROD activity after 2 hr exposure. Three test chemicals apparently induced CYP and EROD activity in the microsome of surf clam. The inducing potentials depend en the test chemicals.