• Title/Summary/Keyword: nutrient culture

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Automatic Measurement of Microbial Growth in Snake-Flask Culture using an Economic and Automated flow injection Analysis Apparatus (경제적인 자동화 FIA 장치를 이용한 진탕플라스크 배양액의 미생물 증식도의 자동측정)

  • 이형춘
    • KSBB Journal
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    • v.19 no.3
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    • pp.241-244
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    • 2004
  • An automated flow injection analysis apparatus manufactured economically was used in the automatic measurement of E. col growth in shake-flask culture of nutrient broth. The detailed measurement of whole growth was possible at intervals of 10 minutes by the automatic measurement system which adopted the sterilized nutrient broth as a carrier solution. Using distilled water as a carrier solution showed less accurate results than nutrient broth.

Effect of Nutrient Nitrogen on the Degradation of Pentachlorophenol by White Rot Fungus, Phanerochaete chrysosporium

  • Chung, Nam-Hyun;Kang, Gu-Young;Kim, Gyu-Hyeok;Lee, Il-Seok;Bang, Won-Gi
    • Journal of Microbiology and Biotechnology
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    • v.11 no.4
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    • pp.704-708
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    • 2001
  • The effect of nutrient nitrogen on the degradation of pentachlorophenol (PCP) by Phanerochaete chrysosporium in a liquid culture was investigated. PCP disappeared at almost the same rate in both nutrient nitrogen-sufficient (NS) and -limited (NL) sttionary cultures. However, more pentachloroanisole (PCA) was accumulated in the NS culture than in the NL culture. The effect of nitrogen on the degradation of PCA was also tested in both cultures. PCA disappeared faster in the NL culture than in the NS culture, indicating that the lower accumulation of PCA during the degradation of PCP in the NL culture was due to the faster degradation of PCA in the NL culture than in the NS culture. In another experiment, PCA was added to shaking cultures rather than stationary cultures to search for any other metabolite(s). While no other metabolite but PCA was found in the NS stationary culture, 2,4,5,6-tetrachloro-2,5-cyclohexadiene-1,4-dione(TCHD) was found as the only indentifiable product in the NL shaking culture. Thus, PCP would appear to be metabolized to TCHD via PCa or directly oxidized to TCHD by lignin peroxidase. Since all the above results indicate that no innocuous metabolite was formed during the degradation of PCP by the fungus, it is quite feasible to use the fungus in the biotreatment of PCP.

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Factors and Recovery of Herbicide Phytotoxicity on Direct-seeded Rice - 2. Phytotoxicity of Herbicide with Nutrient Condition (직파(直播) 벼의 제초제(除草劑) 약해(藥害) 요인(要因)과 회복(回復) 연구(硏究) - 2. 영양(營養) 조건(條件)에 따른 약해(藥害))

  • Im, Il-Bin;Usui, K.
    • Korean Journal of Weed Science
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    • v.17 no.1
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    • pp.24-30
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    • 1997
  • The influence of nutrients on the phytotoxicity of herbicides (bensulfuron-methyl, pyrazosulfuron-ethyl, imazosulfuron, dimepiperate, and molinate) was investigated in controlled-environment growth chamber with direct-seeded rice (Oryza sativa L. cv. Dongjin). The phytotoxicity of bensulfuron-methyl, pyrazosulfuron-ethyl, and imazosulfuron for rice was greater in nutrient culture than in no-nutrient condition. The root growth of rice applied with these herbicides was more inhibited than the shoot growth. The most severe inhibition was obtained with pyrazosulfuron-ethyl application. The growth inhibition of rice applied by dimepiperate was increased under no-nutrient culture condition. Dimepiperate suppressed more remarkably shoot growth than root. Especially the shoot elongation was much more inhibited than the others. The shoot growth inhibition in rice applied by molinate was severer than the root. The shoot growth was reduced under nutrient culture condition, while the root growth was reduced under no-nutrient culture.

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Effect of the Yea-Sacc yeast culture on growth performance, nutrient digestibility and fecal score in weanling pigs

  • Li, Yanjiao;Li, Tianshui;Kim, Inho
    • Korean Journal of Agricultural Science
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    • v.46 no.2
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    • pp.229-237
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    • 2019
  • The influence of dietary supplementation with a yeast culture on growth performance, fecal score and nutrient digestibility was evaluated in weaned pigs in a 6-week feeding trial. A total of 50 weaning pigs with an initial average body weight (BW) of $7.46{\pm}1.60kg$ were randomly allotted into 1 of 2 dietary treatments according to the initial BW. There were 5 replicate pens in each treatment with 5 pigs per pen. The dietary treatments were as follows: 1) control, basal diet (CON) and 2) 0.10% yeast culture, basal diet supplemented with 0.1% yeast culture (YC). The average daily feed intake was significantly improved with the dietary supplementation of the yeast culture compared with the control during phases 1 and 3. Overall (0 to 6 weeks), dietary supplementation with the 0.1% yeast culture had a significant effect on the feed conversion ratio (FCR). There was no significant difference in the fecal score between the CON and YC dietary treatments. In addition, no difference in the apparent total tract digestibility was observed between the CON and YC dietary treatments. Collectively, the results of this study indicate that dietary supplementation of 0.1% yeast only improved the feed intake of weaning pigs; however, yeast culture supplementation did not affect the average daily gain, feed efficiency, total tract digestibility of dry matter, and nitrogen and energy levels as well as the fecal scores.

Effect of Substrate on the Production of Korean Ginseng(Panax ginseng C.A. Meyer) in Nutrient Culture (한국인삼 양액재배시 배지의 영향)

  • Dong Sik Yang;Gung Pyo Lee;Park, Kuen Woo
    • Journal of Bio-Environment Control
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    • v.11 no.4
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    • pp.199-204
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    • 2002
  • To overcome a decrease of Korean ginseng production caused by successive cropping, we have tried to develop a nutrient culture system for Korean ginseng production. For determining the optimal substrate, mixture of sand and TKS-2 (S+T), peatmoss (P), reused rockwool (RR), and granular rockwool (GR) were investigated. The overall physico-chemical properties of RR fell into the reported optimal range for the ginseng cultivation. However, bulk density of S+T was a little higher than that of soil in Korean ginseng fields. The top fresh weight of the ginseng was high in RR and S+T substrates. The root fresh and dry weights in the RR were remarkably greater than that in the conventional soil (CS) of Korean ginseng fields. In terms of ginseng quality, the vitamin C content of ginseng root in nutrient culture was higher than that in CS. However, the contents of crude saponin and total ginsenosides in ginseng between in the nutrient culture and in the soil culture did not show any significant differences.

Reusing Techniques of Nutrient Solution for Recycling Hydroponic Culture of Lettuce (순환식 상추 양액재배시 양액재활용 기술)

  • 이성재;서명훈;이상우;심상연;이수연
    • Journal of Bio-Environment Control
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    • v.8 no.3
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    • pp.172-182
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    • 1999
  • Leaf lettuce(Lacaug sativa L.) was cultivated in deep flow culture to investigate growth and yield in relation to different reusing method of nutrient solution after once cultivation. Five different treatments were allocated to the nutrient solutions - Control(total renewal of solution), NSS(nutrient solution supplement), U control, NSAC(nutrient solution analysis and compensation), NSAC and Humus supply(NSAC with supply of Humus). The pH of solution was kept stable below 7.0 during 4 successive culture in NSAC and NSAC and Humus supply. U was sharply declined in NSS as the number of cultivation was increased. Gmwth and yield of NSAC was similar to those of Control because nutrient elements were kept the balance to the better growth, while the lettuce grown in NSS and EC control was shown lower growth rate. In the nutrient solutions, Content of N $O_3$-N and N $H_4$-N were remarkably decreased after the cultivation in all treatments. Ca and Mg were shown to be accumulated in nutrient solution regardless of culture times and treatments. After the first culture in NSAC and Humus supply, total N and P$_2$ $O_{5}$ content in leaves were lower than any other treatments, but Ca content was higher. Those were not significant as following cultures, and no significant difference of K and Mg content were shown among the treatments.

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Possibility of Cultivation of Pleurotus ostreatus Nutrition by Importing Country (Pleurotus ostreatus 영양원의 수입국별 재배 가능성 검토)

  • Cho, Young-Koo;Park, Youn-Jin;Oh, Tae-Seok;Kim, Chang-Ho;Oh, Min-Kyo;Lee, Young-Soo;Chang, Myung-Jun
    • Korean Journal of Organic Agriculture
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    • v.25 no.4
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    • pp.749-758
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    • 2017
  • This Research was the standardization of nutrient sources by reviewing the possibility of cultivation and analysis of imported nutrient sources used in Pleurotus ostreatus culture. As a first result, the concentration of heavy metals was not detected or trace, and the total nitrogen content of cotton seed pomace was 6.22% in Australia, which was higher than that of Chinese or USA. As a second result of physical properties of nutrient sources, the water holding capacity of beet pulp from Egypt was higher with increasing pressure. The volume water content of US was highest at 400hpa and 500hpa in case of cottonseed meal. Third, the content of total nitrogen was the highest at pH 5.51 and the total nitrogen content was the highest at 2.45, and the C/N ratio was the highest at 28.18. Last result shows that first germination day, yield and biological effciency of all treatments were not significant differnet except T2. These results shows that australian cootton was inappropriate to Pleurotus ostreatus culture. Nevertheless, the safety and standardization of the Pleurotus ostreatus culture medium should be assured by reviewing the media low material importing season, mushroom yield and nutrient sources.

High Cell Density Culture of Anabaena variabilis with Controlled Light Intensity and Nutrient Supply

  • Yoon, Jong-Hyun;Shin, Jong-Hwan;Ahn, Eun-Kyung;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.5
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    • pp.918-925
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    • 2008
  • Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above $10\;{\mu}mol/s/g$ dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.

Selection of Proper Nutrient Solution Existing for the Water Culture of Young Welsh Onion (실파의 수경재배에 적합한 양액종류 선발)

  • Won Jae Hee;Jeon Shin Jae;Kim Sang Soo;Park Kuen Woo
    • Journal of Bio-Environment Control
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    • v.14 no.4
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    • pp.263-268
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    • 2005
  • The purpose of this experiment was to select proper nutrient solution for the water culture of young welsh onion (Allium fistulosum L.). Using two welsh onion varieties, 'Geumjanguedaepa' and 'Tokyokuro', which are good for water culture, four different nutrient solutions which were Chiba Agricultural Experiment Station solution for welsh onion, M's solution for leafy vegetables, Takekawa's solution far welsh onion, and Yamazaki's solution for welsh onion seedling were tested. Among the four nutrient solutions, increments of fresh weight and dry weight per block were the highest in Yamazaki's solution and, in the next, were placed by the order of M's solution, Chiba Agricultural Experiment Station solution and Takekawa's solution. Considering all the other results and fresh weight and dry weight increments, Yamazaki's solution for welsh onion seedling was selected as the best nutrient solution for water culture of young welsh onion.

Analysis of Nutritional Index of Frequently Consumed Fruits in South Korea (한국인 다빈도 섭취 과일의 영양관련 지수 분석)

  • Ryu, Ji-Hyun;Yim, Jung-Eun;Lim, Hyunjung;Choue, Ryowon
    • Journal of the Korean Society of Food Culture
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    • v.29 no.6
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    • pp.659-667
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    • 2014
  • Fruits are good sources of vitamins, minerals, fiber, and phytochemicals, which are known to reduce serum lipids, oxidative damage, and blood pressure as well as improve blood glucose control. The purpose of this study was to estimate nutrient quality indices of fruits by carrying out a critical analysis of pre-existing methods according to their nutritional compositions. Four methods were used to assess the nutrient indices of 26 fruits, which are frequently consumed by Koreans based on the fourth Korean National Health and Nutrition Examination Survey (2009). Naturally nutrient rich score (NNR), nutrient rich food (NRF), nutrient adequacy score (NAS), and nutrient density score (NDS) were used to calculate nutrient quality indices. The Korean Nutrition Society Food Composition database of fruits based on 100 g edible portions was used. The algorithm of each method included the mean percentage of daily values (Dietary Reference Intakes for Koreans, 2010) for particular nutrients based on consumption of 1,900 kcal/day. The relative score indicated that strawberries, kumquat, and lemon had high nutrient quality indices. In addition, mango, lemon, persimmon, strawberry, apricot, and tangerine fruits are rich in antioxidant nutrients such as ${\beta}$-carotene, vitamin C, vitamin E, and selenium. However, scores of nutrient quality indices did not imply that higher scores of particular fruits are superior. We suggest moderate seasonable consumption a variety of fruits. Our results can be used as a reference for consumers when they choose fruits.