• Oncology Letters
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• 제18권2호
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• pp.1189-1198
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• 2019

Prostate cancer (PC) metastasizes to the bone, and a small number of cancer cells, described as cancer stem cells (CSCs), have the ability to differentiate into tumor cells. CSCs are responsible for tumor recurrence and metastases. In the present study, we examined whether ectopic overexpression of CD133, a key molecule maintaining the stability of CSCs in the human PC cell line, LnCaP, caused bone metastasis in a mouse model. Ectopic overexpression of CD133 was induced in LnCaP cells, and CSC-related protein expression was measured. Furthermore, a colony-forming assay was performed to compare results against the blank green fluorescent protein-expressing cells. Furthermore, epithelial to mesenchymal transition-related protein expression, cell migration and wound healing were investigated. To assess the role of CD133 in bone metastasis, CD133-overexpressing LnCaP cells were inoculated into mice via intracardiac injection, and bone metastasis was assessed via histological and immunohistochemical study. In addition, cytokine arrays were used to determine the cytokines involved in bone metastasis. Ectopic overexpression of CD133 in LnCaP cells increased CSC properties such as Oct-4 and Nanog expression and colony-forming ability. Furthermore, epithelial-to-mesenchymal transition (EMT) properties, including decreased E-cadherin and increased vimentin expression, wound gap distance, and cell migration increased. CD133 overexpression led to formation of bone metastatic tumors in mice, consistent with results of hematoxylin and eosin staining. In addition, an increase in expression of the macrophage-migration inhibitory factor was observed at the tumor margin in mice inoculated with CD133+ LNCaP cells. These findings suggest a regulatory role of CD133 in stem cell and EMT properties, and the sustained acquisition of osteolytic features in PC. Therefore, our results may facilitate development of a novel classification system and therapeutic strategies for bone metastasis of PC.

• 한국산림과학회지
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• 제95권1호
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• pp.12-22
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• 2006

자기안내식 해설판의 정보전달 효과를 높이기 위해서는 해설판 글자의 가독성에 대한 이해가 필요하다. 본 연구에서는 해설판의 글자속성에 따른 가독성을 각각 비교하였다. 2004년도 9-11월중 무등산도립공원 내 너덜겅 지역에서 총 1391명의 응답자를 대상으로 설문조사를 실시하였다. 본 연구에서 선정된 5개 글자속성(글자체, 글자정렬, 글줄간격, 글자간격, 한 줄의 글자수)의 가독성 비교 결과, 글자크기 58포인트의 경우 글자체는 'Hy 견명조', 글자정렬은 '양단정렬', 글줄간격은 190%(한글 2002), 글자간격은 10(한글 2002), 한 줄의 글자수는 25자일 때 각 글자속성의 가독성이 최적으로 나타났다. 최적 가독성으로 나타난 각각의 글자속성을 조합하여 제작한 해설판의 글자를 예시하였으며, 가독성과 관련하여 해설판 글자디자인과 추후 연구 문제에 대하여 토의하였다.

• 한국컴퓨터정보학회논문지
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• 제18권10호
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• pp.163-172
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• 2013

본 연구는 유전자알고리즘을 사용하여 표의 크기에 맞게 가장 최적화된 글자로 표현할 수 있는 방법을 실험하였다. 그 결과 셀의 넓이와 높이, 입력받을 글자의 개수를 계산하여 글자 크기, 줄 간격, 자간 간격의 최적의 값을 찾아 길이가 서로 다른 글자를 최적화된 상태로 표현할 수 있도록 폰트를 제공할 수 있었다. 본 연구는 유전자 알고리즘을 통하여 현재 사용하고 있는 다양한 워드프로세스에서 발생되고 있는 셀 고정 상태에서의 글자 최적화 문제에 대한 해결 방법을 제시하였다는데 그 의의가 있다.

• 대한의생명과학회지
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• 제25권1호
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• pp.32-39
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• 2019

Conventional high-flux hemodialysis (HD) is not as good as normal kidney function. Morbidity and mortality rates of patients receiving HD are still very high. To increase mid-to-large molecule clearance by combining diffusion and convection, on-line hemodiafiltration (HDF) is required. The objective of this study was to compare long-term survival rate of patients treated with on-line HDF to those who received conventional high-flux HD by reviewing data from Chonnam National University Hospital (CNUH). We selected patients who attended the 'CUNH dialysis center' and agreed to participate in the study. Overall, 40 patients with ESRD switched from high flux HD to on-line HDF or started on-line HDF from August 2007 to December 2009. Additionally, a total of 42 patients receiving conventional high-flux HD during the same period were enrolled. We then reviewed long-term survival rate of patients receiving on-line HDF over the next seven years. When we compared survival rates for seven years, the survival rate of the group receiving on-line HDF was 65% (26/40) while that of the group receiving the conventional high-flux HD was 54.8% (23/42). Although the number of patients was small to see survival difference clearly by one specific dialysis modality, there was somewhat difference in survival rate between the two groups. Indicators such as anemia, calcium-phosphate metabolism, nutritional status, treatment adequacy, and hospitalization were also improved in the group receiving HDF. Overall, results of our study showed beneficial effects of on-line HDF on clinical outcomes and survival in chronic HD patients.

• 무역상무연구
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• 제22권
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• pp.107-135
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• 2004

The International Standard Banking Practice for the Examination of Documents under Documentary Letters of Credit (ISBP) is the product by the ICC Banking Commission, October 2002. The ISBP is a practical complement to UCP 500, ICC's universally used rules on documentary credits. It explains, in explicit detail, how the rules are to be applied on a day-to-day basis. It fills a needed gap between the general principles announced in the rules and the daily work of the documentary credit practitioner. By using the ISBP, document checkers can bring their practices in line with those followed by their colleagues worldwide. The result should be a significant reduction in the number of documents refused for discrepancies on first presentation. We are convinced that the benefits of the ISBP will not only be of high importance for users of UCP 500, but also that the practices in the ISBP will survive a UCP revision, or will even be included in the next version of the rule (so call "UCP 600"). Though the above-mentioned benefits of the ISBP, there are several troublesome topics that will probably have to wait for a new UCP revision. It will be a challenge for the drafters of a future UCP to find solutions that will further clarify these points.

• Mass Spectrometry Letters
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• 제7권1호
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• pp.1-11
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• 2016

Various efforts have been developed to improve sample preparation steps, which strongly depend on hands-on processes for accurate and sensitive quantitative proteome analysis. In this study, we carried out heating the sample prior to trypsin digestion using an instrument to improve the tryptic digestion process. The heat shock generated by the system efficiently denatured proteins in the sample and increased the reproducibility in quantitative proteomics based on peptide abundance measurements. To demonstrate the effectiveness of the protocol, three cell lines (A human lung cancer cell line (A549), a human embryonic kidney cell line (HEK293T), and a human colorectal cancer cell line (HCT-116)) were selected and the effect of heat shock was compared to that of normal tryptic digestion processes. The tryptic digests were desalted and analysed by LC-MS/MS, the results showed 57 and 36% increase in the number of identified unique peptides and proteins, respectively, than conventional digestion. Heat shock treated samples showed higher numbers of shorter peptides and peptides with low inter-sample variation among triplicate runs. Quantitative LC-MS/MS analysis of heat shock treated sample yielded peptides with smaller relative error percentage for the triplicate run when the peak areas were compared. Exposure of heat-shock to proteomic samples prior to proteolysis in conventional digestion process can increase the digestion efficiency of trypsin resulting in production of increased number of peptides eventually leading to higher proteome coverage.

• 대한의생명과학회지
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• 제18권1호
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• pp.16-21
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• 2012

Although there are many potential cytotoxic molecules released from bacteria, the role of these molecules on the apoptosis of various cancer cells is not well understood. Pseudomonas aeruginosa (P. aeruginosa) is a Gram-negative, aerobic and rod-shaped bacterium, and has a number of virulence factors. To understand the cytotoxic effect of bacterial extracts on the colorectal cancer cell line, HCT 116 cells, we examined alteration of the cell viability, proliferation, cell cycle and apoptosis of HCT 116 cells after treatment with extract of P. aeruginosa (PaE). These cytotoxicity of PaE occurred in a time- and a dose-dependent manners. In addition, PaE arrested the cell cycle of HCT 116 cell in a time-dependent manner. PaE inhibited the protein levels of Bcl-2 and induced the release of cytochrome c from mitochondria of HCT 116 cells. The decrease of procaspase-3 was induced by the treatment of PaE. These results indicate that PaE has a cytotoxicity in HCT 116 cells via the induction of apoptosis associated with mitochondrial pathway. Therefore, PaE may used as the potential target for the treatment of colorectal cancer.

• 무역상무연구
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• 제20권
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• pp.317-341
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• 2003

The publication International Standard Banking Practice for the Examination of Documents under Documentary Letters of Credit (ISBP) is the product by a task force of the ICC Banking Commission. The ISBP is a practical complement to UCP 500, ICC’s universally used rules on documentary credits. The ISBP does not amend the UCP. It explains, in explicit detail, how the rules are to be applied on a day-to-day basis. It fills a needed gap between the general principles announced in the rules and the daily work of the documentary credit practitioner. By using the ISBP, document checkers can bring their practices in line with those followed by their colleagues worldwide. The result should be a significant reduction in the number of documents refused for discrepancies on first presentation.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제6권5호
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• pp.496-504
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• 2000

웹 게이트웨이는 WWW와 데이터베이스를 연동하는 인터페이스의 핵심을 차지하는 부분이다. KORDIC(Korea Research & Development Information Center)에서 개발된 기존의 KRISTAL-II정보 검색 시스템[1]은 단순 CGI 구조의 웹 게이트웨이를 채택하고 있다. 이러한 구조의 웹 게이트웨이는 구현이 쉬우나 대규모의 검색 요구를 효율적으로 처리하기 에는 부적합한 구조라는 단점이 있다. 웹을 통한 검색 요구가 RJWA점 증가하는 추세임을 감안할 때, 대규모의 검색 요구를 효율적으로 처리할 수 있는 웹 게이트웨이를 개발하는 것은 매우 중요한 일이다. 본 논문에서는 기존 KRISTAL-II 웹 게이트웨이의 단순 CGI 구조를 대규모의 검색 요구의 효율적인 처리에 적합한 3-tier 클라이언트-서버 구조로 개선하였다. 실험을 통해 제안하는 새로운 웹 게이트웨이의 성능을 평가한다.

• 대한의생명과학회지
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• 제10권2호
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• pp.85-92
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• 2004

Immortalization of primary corneal cells has influence on pharmacy, medical and biological fields. Especially, investigation of immortalization mechanism using viral oncoproteins is useful for medical treatments, and these cell lines will be useful materials for toxic test of medical supplies and cell biological experiments. Rabbit corneal fibroblasts in culture undergo a finite number of divisions before they reach a terminally non-proliferating state known as replicative senescence. Therefore, we attempted to induce immortalization of rabbit corneal fibroblasts with SV 40 large T antigen. As a result of experiment, expression of SV 40 large T antigen was confirmed, and expression of proteins related to cell cycle repressor was decreased in the transfection group compared with non-transfection group. According to the results of cell cycle phase distribution test, SV 40 large T antigen-transfected cells had obtained higher proliferation rate than primary cells. It was confirmed that during induction of immortalization, SV 40 large T antigen was not able to increase telomerase activity. In conclusion, we made a rabbit corneal fibroblast cell line with SV40 large T antigen. This cell line will be useful for further studies of mammalian fibroblast biology, particularly with regard to angiogenesis and malignant transformation. In addition, this cell line offers opportunity for testing potential therapeutics and can be used for toxicity tests of materials or cosmetics. In the future, our cell line can potentially be utilized in a wide range of biology related fields.