• Journal of Computing Science and Engineering
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• v.8 no.2
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• pp.94-106
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• 2014

Schema matching is widely used in many applications, such as data integration, ontology merging, data warehouse and dataspaces. In this paper, we propose a novel matching technique that is based on the order of attributes appearing in the schema structure of query results. The appearance order embodies the extent of the importance of an attribute for the user examining the query results. The core idea of our approach is to collect statistics about the appearance order of attributes from the query logs, to find correspondences between attributes in the schemas to be matched. As a first step, we employ a matrix to structure the statistics around the appearance order of attributes. Then, two scoring functions are considered to measure the similarity of the collected statistics. Finally, a traditional algorithm is employed to find the mapping with the highest score. Furthermore, our approach can be seen as a complementary member to the family of the existing matchers, and can also be combined with them to obtain more accurate results. We validate our approach with an experimental study, the results of which demonstrate that our approach is effective, and has good performance.

• Microbiology and Biotechnology Letters
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• v.41 no.3
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• pp.278-283
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• 2013

A gram-positive, rod-shaped, spore-forming, motile thermophilic bacterium was isolated from a sterilization oven. The microorganism GWE1, formally named Geobacillus wiegelii identified as a member of the genus Geobacillus. GWE1 grew under aerobic conditions of between $60-80^{\circ}C$ (optimum $670^{\circ}C$), in a pH range of 3.0-8.0 (optimum $pH^{70^{\circ}C}$ 5.8), and between 0 and 2 M NaCl (optimum 0.3 M). The membrane polar lipids were dominated by branched saturated fatty acids, which included as the major constituents; iso-15:0 (13.3%), 16:1(${\omega}7$) (12.8%), 16:0 (28.5%), iso-17:0 (13.5%) and anteiso-17:0 (12.3%). The DNA G+C content was 47.2 mol% (determined by HPLC). The 16S rRNA gene sequence of GWE1 showed a high similarity with Geobacillus caldoxylosilyticus (97%). However, the level of DNA-DNA relatedness was only 58%. These data suggest that GWE1 is probably a novel specie of the genus Geobacillus.

• Molecules and Cells
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• v.37 no.6
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• pp.441-448
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• 2014

Inflammasomes are specialized signaling platforms critical for the regulation of innate immune and inflammatory responses. Various NLR family members (i.e., NLRP1, NLRP3, and IPAF) as well as the PYHIN family member AIM2 can form inflammasome complexes. These multiprotein complexes activate inflammatory caspases (i.e., caspase-1) which in turn catalyze the maturation of select pro-inflammatory cytokines, including interleukin (IL)-$1{\beta}$ and IL-18. Activation of the NLRP3 inflammasome typically requires two initiating signals. Toll-like receptor (TLR) and NOD-like receptor (NLR) agonists activate the transcription of pro-inflammatory cytokine genes through an NF-${\kappa}B$-dependent priming signal. Following exposure to extracellular ATP, stimulation of the P2X purinoreceptor-7 ($P2X_7R$), which results in $K^+$ efflux, is required as a second signal for NLRP3 inflammasome formation. Alternative models for NLRP3 activation involve lysosomal destabilization and phagocytic NADPH oxidase and /or mitochondria-dependent reactive oxygen species (ROS) production. In this review we examine regulatory mechanisms that activate the NLRP3 inflammasome pathway. Furthermore, we discuss the potential roles of NLRP3 in metabolic and cognitive diseases, including obesity, type 2 diabetes mellitus, Alzheimer's disease, and major depressive disorder. Novel therapeutics involving inflammasome activation may result in possible clinical applications in the near future.

• Journal of Microbiology and Biotechnology
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• v.16 no.9
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• pp.1429-1433
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• 2006

Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of $^{15}N$ and/or $^{13}C$ labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted ${\alpha}$-helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.

• Bulletin of the Korean Chemical Society
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• v.30 no.8
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• pp.1827-1831
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• 2009

Ligand molecules that can recognize and interact with cancer cell surface marker proteins with high affinity and specificity should greatly aid the development of novel cancer diagnostics and therapeutics. HER-2/ErbB2/Neu (HER-2), a member of the epidermal growth factor receptor family, is specifically overexpressed on the surface of breast cancer cells and serves as both a useful biomarker and a therapeutic target for breast cancer. In this study, we aimed to isolate RNA aptamers that specifically bind to a HER-2-overexpressing human breast cancer cell line, SK-BR-3, using Cell-SELEX strategy. The selected aptamers showed strong affinity to SK-BR-3, but not to MDAMB- 231, a HER-2-underexpressing breast cancer cell line. In addition, we confirmed the specific targeting of HER-2 receptor by aptamers using an unrelated mouse cell line overexpressing human HER-2 receptor. The HER-2-targeting RNA aptamers could become a useful reagent for the development of breast cancer diagnostics and therapeutics.

• Structural Engineering and Mechanics
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• v.15 no.4
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• pp.415-436
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• 2003

The combination of spatial latticed structures (hereafter SLS) and flexible cables, the cable-stayed spatial latticed structures (hereafter CSLS) can cross longer span. According to variation principle, a novel geometric nonlinear formulation for 3-D bar elements considering large displacement and infinitesimal rotation increments with second-order precision is developed. The cable nonlinearity is investigated and it is taken that the secant modulus method can be considered as an exact method for a cable member. The tower column with which the cables link is regarded as a special kind of beam element, and, a new simplified stiffness formulation is presented. The computational strategies for the nonlinear dynamic response of structures are given, and the ultimate load carrying capacities and seismic responses are analyzed numerically. It is noted that, compared with corresponding spatial latticed shells, the cable-stayed spatial latticed shells have more strength and more stiffness, and that the verical seismic responses of both CSLS and CLS are remarkably greater than the horizontal ones. In addition, the computation shows that the stiffness of tower column influences the performance of CSLS to a certain extent and the improvement of structural strength and stiffness of CSLS is relevant not only to cables but also to tower columns.

• Advances in Computational Design
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• v.1 no.3
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• pp.253-264
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• 2016

Based on the variation of strain along the cross section, any region in a structural member can be classified into two regions namely, Bernoulli's region (B-region) and Disturbed region (D-region). Since the variation of strain along the cross section for a B-region is linear, well-developed theories are available for their analysis and design. On the other hand, the design of D-region is carried out based on thumb rules and past experience due to the presence of nonlinear strain distribution. Strut-and-Tie method is a novel approach that can be used for the analysis and design of both B-region as well as D-region with equal importance. The strut efficiency factor (${\beta}_s$) is needed for the design and analysis of concrete members using Strut and Tie method. In this paper, equations for finding ${\beta}_s$ for bottle shaped struts in concrete deep beams (a D-region) with and without steel fibres are developed. The effects of transverse reinforcement on ${\beta}_s$ are also considered. Numerical studies using commercially available finite element software along with limited amount of experimental studies were used to find ${\beta}_s$.

• Journal of Information Processing Systems
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• v.14 no.4
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• pp.926-940
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• 2018

In clustering-based approaches, cluster heads closer to the sink are usually burdened with much more relay traffic and thus, tend to die early. To address this problem, distance-aware clustering approaches, such as energy-efficient unequal clustering (EEUC), that adjust the cluster size according to the distance between the sink and each cluster head have been proposed. However, the network lifetime of such approaches is highly dependent on the distribution of the sensor nodes, because, in randomly distributed sensor networks, the approaches do not guarantee that the cluster energy consumption will be proportional to the cluster size. To address this problem, we propose a novel approach called CACD (Clustering Algorithm Considering node Distribution), which is not only distance-aware but also node density-aware approach. In CACD, clusters are allowed to have limited member nodes, which are determined by the distance between the sink and the cluster head. Simulation results show that CACD is 20%-50% more energy-efficient than previous work under various operational conditions considering the network lifetime.

• Journal of Power Electronics
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• v.13 no.3
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• pp.419-428
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• 2013

The modular multilevel cascade converter (MMCC) is a new family of multilevel power converters with modular realization and a cascaded pattern for submodules. The MMCC family can be classified by basic configurations and submodule types. One member of this family, the Hexverter, is configured as Double-Delta Full-Bridge (DDFB). It is a novel multilevel AC/AC converter with direct power conversion and comparatively fewer required components. It is appropriate for connecting two three-phase systems with different frequencies and driving an AC motor directly from a utility grid. This paper presents the dq model of a Hexverter with both of its AC systems by state-space representation, which then simplifies the continuous time-varying model into a periodic discrete time-invariant one. Then a generalized multivariable optimal control strategy for regulating the Hexverter's independent currents is developed. The resulting control structure can be adapted to other MMCCs and is flexible enough to include other control criterion while guaranteeing the original controller performance. The modeling method and control design are verified by simulation results.

• Development and Reproduction
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• v.22 no.1
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• pp.65-72
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• 2018

Cyclic AMP-response element binding protein zhangfei (CREBZF), a member of ATF/CREB (activating transcription factor/ cAMP response element binding protein) family, regulates numerous cellular functions and development of cells by interacting transcription factors. This study discovered the expression pattern of CREBZF in seminiferous tubule of testes during the postnatal development of mice. In testis, CREBZF mRNA expression was the highest among other organs. Immunofluorescence analyses showed that the CREBZF was specifically expressed on spermatocyte but not in spermatogonia and Sertoli cells in seminiferous epithelium of mouse testis. Semi-quantitative polymerase chain reaction (PCR) analysis showed that CREBZF transcript level was significantly elevated during postnatal development of mouse testis. Confocal imaging analysis indicated that the protein expression of CREBZF in seminiferous tubule remained low until postnatal day (PD) 14, and was dramatically increased in PD 21. Interestingly, only one type of the spermatocyte expressed CREBZF specifically among SCP3-positive spermatocytes. Taken together, these results suggest that CREBZF may be novel putative marker of the spermatocyte and regulate meiosis during postnatal development of mice.