• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.187.1-187.1
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• 2003

Type 2 diabetes is characterized by high level of blood glucose and insulin and impaired action. In recent years, the treatment of type 2 diabetes has been revolutionized with the advent of thiazolidinedione (TZD) class of molecules that ameliorate insulin resistance and thereby normalize elevated blood glucose levels. These TZDs are synthetic, high-affinity ligands of peroxisome proliferator activated receptor-gamma (PPAR${\gamma}$); a member of the nuclear receptor family that controls the expression of genes in the target tissues of insulin action. (omitted)

• 한국발생생물학회지:발생과생식
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• 제21권3호
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• pp.237-247
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• 2017

Mammalian spermatogenesis occurs in a precise and coordinated manner in the seminiferous tubules. One of the attempts to understand the detailed biological process during mammalian spermatogenesis at the molecular level has been to identify the testis specific genes followed by study of the testicular expression pattern of the genes. From the subtracted cDNA library of rat testis prepared using representational difference analysis (RDA) method, a complimentary DNA clone encoding type III member of a DnaJ family protein, DnaJC18, was cloned (GenBank Accession No. DQ158861). The full-length DnaJC18 cDNA has the longest open reading frame of 357 amino acids. Tissue and developmental Northern blot analysis revealed that the DnaJC18 gene was expressed specifically in testis and began to express from postnatal week 4 testis, respectively. In situ hybridization studies showed that DnaJC18 mRNA was expressed only during the maturation stages of late pachytene, round and elongated spermatids of adult rat testis. Western blot analysis with DnaJC18 antibody revealed that 41.2 kDa DnaJC18 protein was detected only in adult testis. Immunohistochemistry study further confirmed that DnaJC18 protein, was expressed in developing germ cells and the result was in concert with the in situ hybridization result. Confocal microscopy with GFP tagged DnaJC18 protein revealed that it was localized in the cytoplasm of cells. Taken together, these results suggested that testis specific DnaJC18, a member of the type III DnaJ protein family, might play a role during germ cell maturation in adult rat testis.

• 정보처리학회논문지C
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• 제9C권4호
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• pp.597-604
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• 2002

멀티캐스트 통신에서 그룹에 새로운 멤버가 가입하거나 기존의 멤버가 탈퇴하는 경우 멤버들이 사용중인 그룹 키는 갱신되어야 한다. 이러한 절차는 완전 전방향과 후방향 비밀성을 보장하기 의해 필요하다(8). 불행하게도, 빈번한 멤버쉽이 변경되는 대규모 그룹에서의 키 갱신은 그룹을 확장하는데 커다란 장애가 된다. 본 논문에서는 대규모 동적 그룹에서 그룹 키를 효율적으로 분배할 수 있는 새로운 방식을 제안한다. 기존의 안전한 멀티캐스트 프로토콜과는 달리, 제안된 프로토콜은 키 갱신의 빈도와 계산에 따른 오버헤드가 전체 그룹의 크기가 아닌 서브그룹의 크기에 따라 영향을 받기 때문에 대규모 그룹으로 확장이 가능하며, 그룹 접근 제어를 수행하는 서브그룹 관리자가 송신자에 의해 전송된 멀티캐스트 데이터에 접근할 수 없도록 하는 메카니즘을 제공한다. 또한 무선 컴퓨팅 환경에서 프라이버시를 보장할 수 있는 보안 서비스를 제공한다.

• 한국응용과학기술학회지
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• 제40권4호
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• pp.881-889
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• 2023

Hymenobacter 속(genus)은 Bacteroidota 문(phylum), Hymenobacteraceae 과(family)의 대표 속(type genus)이다. 이 속에 속하는 세균들은 붉은색 색소를 함유하는 그람 음성 간균으로서, 자연계의 다양한 환경에서 분리되고 있다. 본 연구에서 붉은색 색소를 함유하는 그람 음성 간균이 경남 창원시 소재 창원대학교 교내의 연못에서 분리되었고, 이 세균은 균주 B2로 명명되었다. 균주 B2를 계통분석 및 생화학적으로 분석한 결과, Hymenobacter 속에 속하는 것으로 밝혀졌다. 이 세균의 16S rRNA 유전자 염기서열을 genbank의 BLAST로 분석해 본 결과, 다른 어떠한 세균과도 16S rRNA 유전자 염기서열의 상동성이 새로운 미생물로 인정되는 기준인 98.7%보다 낮은 것으로 나타났다. 균주 B2의 지방산을 분석해 본 결과, 주된 지방산은 summed feature 3(C_16:1 ω7c and/or C_16:1 ω6c, 22.8%), iso-C_15:0(16.2%), anteiso-C_15:0(12.9%), C_16:1ω5c(12.4%) 및 summed feature 4 (iso-C_17:1 I/anteiso-C_17:1)(9.5%)인 것으로 밝혀졌는데, 결과적으로 균주 B2의 지방산 함량은 다른 Hymenobacter 종들의 지방산 함량과 뚜렷한 차이가 있는 것을 알 수 있었다. 이 세균의 16S rRNA 유전자 염기서열은 genbank에 accession number OQ318247로 등록되었다.

• 한국구조물진단유지관리공학회 논문집
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• 제23권3호
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• pp.92-95
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• 2019

본 논문에서는 압분광법을 기반으로 강재에 작용하는 응력을 비파괴/비접촉식으로 측정할 수 있는 새로운 방법은 제안하고, 이에 대한 가능성을 실험적으로 검증하였다. 분광법은 일반적으로 대상물의 화학적 성분을 분석하는데 주로 사용되며, 토목분야에서는 강재의 부식을 판단하는데 일부 사용되고 있다. 압분광법은 대상물이 하중을 받을 때 측정된 스펙트럼이 하중이 없는 상태에서 측정된 스펙트럼으로부터 이동(Shift)되는 현상인, 압분광현상을 기반으로 응력을 측정하는 방법이다. 여기서, 응력-스펙트럼 이동 관계를 선형으로 가정하였을 때, 압분광 계수를 도출할 수 있으며, 이를 통하여 현재상태의 응력을 측정할 수 있다. 해당 기술은 레이저를 기반으로한 비접촉식 응력측정 기술로써, 최근에 철도 구조물에 대한 응력 측정을 시작으로 다양한 토목구조물의 응력측정에 대한 적용이 시도되고 있다. 본 연구에서는 이와 같은 압분광법 기술을 이용하여 강구조물의 응력을 측정할 수 있도록, 용사코팅을 이용하여 알루미나를 구조용 강재표면에 도포하고, 일축압축 하중시험을 수행하여 각 하중단계에서의 스펙트럼 이동(Shift)값을 확인하였다. 이를 통하여, 각 하중값과 스펙트럼 이동값이 선형관계임을 확인하였다. 따라서, 이와 같은 선형적인 관계로 부터, 1차 선형식의 상수값인 압분광 계수를 도출하고, 이를 통하여 강재에 작용하는 응력을 측정할 수 있을 것으로 기대된다.

• Animal Bioscience
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• 제35권12호
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• pp.1827-1838
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• 2022

Objective: The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), progressive motility after freezing (FPM), and the difference between PM and FPM. The respective DNA samples from these stallions were genotyped using Axiom Equine Genotyping Array. Results: We performed a GWAS search for single nucleotide polymorphism (SNP) markers and potential genes related to motility properties of frozen-thawed semen in the stallions of various breeds. As a result of the GWAS analysis, two SNP markers, rs1141327473 and rs1149048772, were identified that were associated with preservation of the frozen-thawed stallion sperm motility, the relevant putative candidate genes being NME/NM23 family member 8 (NME8), olfactory receptor family 2 subfamily AP member 1 (OR2AP1), and olfactory receptor family 6 subfamily C member 4 (OR6C4). Potential implications of effects of these genes on sperm motility are herein discussed. Conclusion: The GWAS results enabled us to localize novel SNPs and candidate genes for sperm motility in stallions. Implications of the study for horse breeding and genetics are a better understanding of genomic regions and candidate genes underlying stallion sperm quality, and improvement in horse reproduction and breeding techniques. The identified markers and genes for sperm cryotolerance and the respective genomic regions are promising candidates for further studying the biological processes in the formation and function of the stallion reproductive system.

• BMB Reports
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• 제43권2호
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• pp.97-102
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• 2010

Clenbuterol, a $\beta_2$-adrenoceptor agonist, has been proven to be a powerful repartition agent that can decrease fat deposition. Based on results from our previous cDNA microarray experiment of pig clenbuterol administration, a novel up-regulated EST was full-length cloned (4859 bp encoding 1041 amino acids) and found to be the pig homolog of large tumor suppressor 2 (Lats2). We mapped pig Lats2 to chromosome 11p13-14 by using FISH, and western blotting demonstrated that pig Lats2 protein was most abundant in adipose. In Drosophila, Lats2 ortholog was reported as a key component of the Hippo pathway which regulates cell differentiation and growth. Here, we show that pig Lats2 exhibit inverted expression to YAP1, another member of the Hippo pathway which positively regulates cell growth and proliferation, during the differentiation of 3T3-L1 preadipocytes. Our results suggested that Lats2 may involve in Hippo pathway regulating the fat reduction by inhibiting adipocyte differentiation and growth.

• International Journal of Oral Biology
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• 제33권2호
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• pp.71-76
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• 2008

The neurotrophin plays an important role in the development, differentiation and survival of the nervous system in vertebrates. It exerts its cellular effects through two different receptors, the Trk receptor tyrosine kinase neurotrophin receptor and the p75 neurotrophin receptor, a member of the tumor necrosis factor receptor superfamily. Trk and p75 neurotrophin receptors utilize specific target proteins to transmit signals into the cell. An ankyrin-rich membrane spanning protein (ARMS) was identified as a new p75 interacting protein and serves as a novel downstream target of p75 neurotrophin receptor. We sought to delineate the interaction between p75 and ARMS by deletion constructs of p75 and green fluorescent protein (GFP)-tagged ARMS. We examined the interaction between these two proteins after overexpressing them in HEK-293 cells. Using both Western blot analysis and immunocytochemistry followed by confocal laser scanning microscopy, we found out that the intracellular domain of the p75 neurotrophin receptor was important for the interaction with ARMS. The results from this study suggest that ARMS may play an important role for mediating the signals from p75 neurotrophin receptor into the cell.

• BMB Reports
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• 제39권5호
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• pp.626-635
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• 2006

Lysophosphatidic acid acyltransferase (LPAAT) is an intrinsic membrane protein that catalyzes the synthesis of phosphatidic acid (PA) from lysophosphatidic acid (LPA). It is well known that LPAAT is involved in lipid biosynthesis, while its role in tumour progression has been of emerging interest in the last few years. To date, seven members of the LPAAT gene family have been found in human. Here we report a novel LPAAT member, designated as LPAAT-theta, which was 2728 base pairs in length and contained an open reading frame (ORF) encoding 434 amino acids. The LPAAT-theta gene consisted of 12 exons and 11 introns, and mapped to chromosome 4q21.23. LPAAT-theta was ubiquitously expressed in 18 human tissues by RT-PCR analysis. Subcellular localization of LPAAT-theta-EGFP fusion protein revealed that LPAAT-theta was distributed primarily in the endoplasmic reticulum (ER) of COS-7 cells. Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells.

• BMB Reports
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• 제53권4호
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• pp.206-211
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• 2020

Vascular smooth muscle cells (VSMCs) are a unique cell type that has unusual plasticity controlled by environmental stimuli. As an abnormal increase of VSMC proliferation is associated with various vascular diseases, tight regulation of VSMC phenotypes is essential for maintaining vascular homeostasis. Hypoxia is one environmental stress that stimulates VSMC proliferation. Emerging evidence has indicated that microRNAs (miRNAs) are critical regulators in the hypoxic responses of VSMCs. Therefore, we previously investigated miRNAs modulated by hypoxia in VSMCs and found that miR-1260b is one of the most upregulated miRNAs under hypoxia. However, the mechanism that underlies the regulation of VSMCs via miR-1260b in response to hypoxia has not been explored. Here we demonstrated that hypoxia-induced miR-1260b promotes VSMC proliferation. We also identified growth differentiation factor 11 (GDF11), a member of the TGF-β superfamily, as a novel target of miR-1260b. miR-1260b directly targets the 3'UTR of GDF11. Downregulation of GDF11 inhibited Smad signaling and consequently enhanced the proliferation of VSMCs. Our findings suggest that miR-1260b-mediated GDF11-Smad-dependent signaling is an essential regulatory mechanism in the proliferation of VSMCs, and this axis is modulated by hypoxia to promote abnormal VSMC proliferation. Therefore, our study unveils a novel function of miR-1260b in the pathological proliferation of VSMCs under hypoxia.