• Korean Journal of Plant Tissue Culture
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• v.28 no.2
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• pp.87-90
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• 2001

BcGRl gene encoding cytosolic glutathione reductase of Chinese cabbage (Brassica campestris var. Pekinensis cv. Seoul) was placed under the control of the CaMV 35S promoter and introduced into tobacco (Nicotiana tabacum L. cv. Samsun) via Agrobacterium-mediated transformation. T$_{0}$ 32 independent plants transformed with BcGRl gene were selected with kanamycin and they were confirmed by polymerase chain reaction (PCR) and Southern blot analysis. Northern blot analysis revealed that the constitutive expression of BcGRl gene and there was no relationship between the copy number of introduced gene and the levels of BcGRl transcripts.

• Korean Journal of Plant Tissue Culture
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• v.21 no.1
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• pp.55-58
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• 1994

Agrobacterium tumefaciens LABA4404 harboring plant binary vector, pBI121, was used for genetic transformation of lettuce (Lactuca sativa t.). Cotyledon segments were infected with A. tumefaciens LBA4404 by cocultivation method and regenerated. Regenerated letture was subject to molecular analyses for integration into plant nuclear genome and expression of ${\beta}$-glucumnidase (GUS) gene. Southern and Northern blot analyses demonstrated that GUS gene was integrated into plant nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by specetrophotometric assay of GUS activity.

• Proceedings of the Botanical Society of Korea Conference
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• 1999.04a
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• pp.10-10
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• 1999

• Journal of Plant Biotechnology
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• v.40 no.4
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• pp.198-202
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• 2013

To produce transgenic cucumber expressing Nit gene coffering abiotic resistance, the cotyledonary-node explants of cucumber (cv. Eunsung) were inoculated with A. tumefaciens transformed with pPZP211 or pCAMBIA2300 carrying Nit gene, that has cis-acting element involved in resistance to various abiotic environmental stresses. After co-cultivation, the procedures of selection, shoot initiation, shoot elongation, and plant regeneration were followed by cotyledonary-node transformation method (CTM, Jang et al. 2011). The putative transgenic plants were selected when shoots were grown to a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 100 mg/L paromomycin as a selectable agent. The confirmation of transgenic cucumber was based on the genomic PCR, Southern blot analysis, RT-PCR, and Northern blot analysis. A 105 shoots (4.12%) selected from the selection mediums were obtained from 2,547 explants inoculated. Of them, putative transgenic plants were only confirmed with 45 plants (1.77%) by genomic PCR analysis. Transgenic plants showed that the Nit genes integrated into each genome of 39 plants (1.53%) by Southern blot analysis, and the expression of gene integrated into cucumber genome was only confirmed at 6 plants (0.24%) by RT-PCR and Northern blot analysis. These results lead us to speculate that the genes were successfully integrated and expressed in each genome of transgenic cucumber.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.100.1-100
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• 2002

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2005.04a
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• pp.91-91
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• 2005

• Journal of Plant Biology
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• v.28 no.4
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• pp.297-304
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• 1985

Two Korean species of Centroceras K tzing, Ceramiaceae was investigated taxonomically. C. clavulatum (Ag.) Montagne collected at several sited along the coast of Korea was characterized by regular dischotomous branches with whorl spines at every node, whereas, C. distichum Okamura collected at Soando in the southern coast was by alternate branches with gland cells around nodes. Biogeographic data show that Korea is almost northern limit in distribution of the former species.

• Plant Biotechnology Reports
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• v.3 no.4
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.7-7
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• 2010

Korea is one of the Northeast Asian countries in the Northern climatic zone. It is wide spread from north to south so that can be affected by various kinds of plants flora of the continent of Asia. Korea has complicated topography, mountains and hills included by an alpine belt. According to the distribution of plants, it can be classified to 5 areas; Northern part, Central part, Southern part, JeJuDo(濟州道) and UlLungDo(鬱陵島). Nakai of Japan, reported Plants in Korean peninsula as 3176 species, 841 varietal species and 174 varieties in "A Synoptical sketch of Korean flora". Lee of Korea, reported 3409 species, 6 sub-species, 756 varietal species and 287 varieties in "Korean Plants Resources". Isidoja(石戶谷) of Japan, simply described crude drug names, scientific names, effects, etc. of 45 species of Korean Medicinal Plants in the book "Journal of Jo-Seon Pharmacy(朝鮮藥學會會報)" third edition (published in 1925) and also explained 250 species of crude drug collected in Manchuria, Mongolia and Korean peninsula in the book "Medicinal plants in Northern Asia(北支那의 藥草)"(1931). Im and Jung organized 227 species of Medicinal Plants in "Wild Medicinal Plants from Jo-Seon(北支那의 藥草)" and it is said that 1000 species of plants can be used for medicinal purposes in Korea.

• Korean Journal of Plant Resources
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• v.10 no.2
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• pp.107-113
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• 1997

Transformants of White Clover(Trifolium repens L.) were efficiently produced from immature seed derived callus cocultivated with Agrobacterium twnefaciens LBA4404 harboring plant binary vector. pBI121, using acetosyringone. The mean frequencies of transformants on the two kanamycin-containing media were 16 to 19% when the immature seed-derived calli were infected with bacteria cultured in the presence of 100$\mu$M acetosyringone compared with 7% in media without acetosyringone. Transgenic white clover was subject to molecular analysis for integration into plant nuclear genome and expression of $\beta$-glucuronidase(GUS) gene. PCR and Northern blot analyses demonstrated that GUS gene was integrated into white clover nuclear genome and expressed into its mRNA. The expression of GUS gene into its protein was confirmed by spectrophotometric assay of GUS activity.