• Journal of Nutrition and Health
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• v.30 no.9
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• pp.1102-1108
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• 1997

The purpose of this study was to find the extent of lipid peroxidation of erythrocytes in cigarette smokers, and to determine the relative effectiveness of $\beta$-carotene, canthaxanthin , and $\alpha$-tocopherol as antioxidants. Thirty smokers and 30 nonsmokers participated in this study . No significant differences according to age, sex, and height were shown. Cigarette smokers in this study had higher hemoglobin concentrations and more oxidation of hemoglobin than non-smokers. In addition, the erythrocytes of cigarette smokers had significantly higher MDA concentrations than crythrocytes of nonsmokers, which suggests that smokers may have tocopherol were studied in vitro by measuring the concentration of malondialdehyde(MDA) and precent hemolysis of erythrocytes. The addition of any antioxidant to erythrocytes significantly decreased MDA concentrations(p<0.05) while antioxidants showed nonsignificant inhibition of hemolysis. Among the antioxidant used in this study, canthaxanthin showed the greatest inhibition of both lipid peroxidationand hemolysis. Meanwhile, $\alpha$-tocopherol showed potent inhibition of lipid peroxidation, but not of hemolysis.

• Journal of Food Hygiene and Safety
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• v.14 no.2
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• pp.129-133
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• 1999

This study was designed to observe the effects of T-2 toxin on total cholesterol and lipid concentration in rat serum. T-2 toxin is a secondary metabolite produced by Fusarium sp. which is often found on agricultural products including cereals, and it is a causal material of liver injuries in cattle and humans. When we fed rats with standard diet treated with T-2 toxin, the body weight and feed consumption of rats treated T-2 toxin were decreased. As the results of lipid analysis, the concentrations of total cholesterol and free cholesterol in serum of treated rats were increased compared to non-fed control group, On the other hand, the levels of triglyceride and phospholipid in the serum of T-2 toxin treated experimental groups were declined. In conclusion, T-2 toxin largely influenced on the total cholesterol and lipid levels in rat serum.

• Journal of Nutrition and Health
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• v.29 no.5
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• pp.489-498
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• 1996

In order to investigate the effect of smoking on serum lipid pattern and nutrient intake, the subjects, 134 smokers and 113 nonsmokers, were selected from college men student in Kwangju area. We examined the serum lipid pattern of 15 smokers and 18 non-smokers who agreed to venipunture among the subjects and also examined nutrients intake. The results obtained were as follows ; Mean concentrations of 새심 cholesterol, HDL-cholesterol and LDL-cholesterol were 187$\pm$12.1mg/dl, 41.5$\pm$1.7mg/dl, and 87.3$\pm$7.4mg/dl in smokers and 182.6$\pm$8.2mg/dl, 45.5$\pm$1.5mg/dl, and 96.3$\pm$4.3mg/dl in nonsmokers, respectively. The total energy intake was 2264kcal/day and mean constitutional rationof protein, fat and carbohydrate was 12.7 : 24.8 : 62.5 in smokes and 2287kcal/day, 14.1 :25.4 : 60.5 in nonsmokers, respctively. Mena daily intakes of calcium, iron, vitamin C and vitamin A were 612.2mg, 15.7mg, 48.2mg 2029.4 IU in smokers and 629.4mg, 15.3mg, 68.4mg 2339.1 IU in nonsmokers, respectively. Vitamin C intake was significantly different at p<0.05 between the smokers and nonsmokers. Intakes of cholesterol, SFA and MUFA were not significantly different between the two groups. Mean daily PUFA intake of smokers and nonsmokers were 5.5g and 6.9g respectively. PUFA intake was significantly different at p<0.05 between the two groups. The results of this study showed that smoking have a tendency to raise coronary heart disease occurrence in the respect of serum lipid levels and vitamin C, PUFA intake were significantly different.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.10
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• pp.1433-1439
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• 2000

An experiment was carried out with 48 IVRI 2CQ rats 6-8 week old, weighing 50-100 g, to study the effect of lead exposure on antioxidant defense, lipid peroxide level, status of thiol groups and concentration of lead in the liver and kidneys at the end of the exposure and also after withdrawal of lead administration. Twenty four rats were given lead at a daily dose rate of 1 mg lead/2 ml of distilled water/kg body weight as lead acetate solution intraperitoneally for a period of 30 days. Another 24 control rats received 2 ml of sterile normal saline solution (0.85% NaCl)/kg body weight in an identical manner. A many-fold increase in concentration of lead was associated with a non-significant (p>0.05) decrease in the activities of superoxide dismutase (SOD) in the liver (27%) and kidneys (12%) and catalase in kidneys (22%). A significant (p<0.05) increase in lipid peroxide level was recorded in the liver (40%) compared with control values. There were significant (p<0.05) decreases in the total thiol and protein bound thiol contents in liver and an increase in non-protein bound thiol groups in the kidneys of lead exposed rats. During the 10 day observation period after withdrawal of lead administration, no significant change was observed with respect to any of the above parameters indicating that a 10 day withdrawal period was not enough for restoration of normality. It is concluded that the magnitude of response and the resultant changes in the lipid peroxide concentration, and the activities of SOD and catalase were not identical in the liver and kidneys of lead-exposed rats.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.3
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• pp.1359-1366
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• 2013

The purpose of this study was to examine the blood pressure, serum lipid levels and obesity indices according to smoking status, and the effects of smoking cessation on blood pressure, serum lipid and obesity indices. The 2,230 male workers aged 30-59 years were observed the age, smoking status, SBP, DBP, TC, TG, HDL-C, LDL-C, AI, BMI, body fat rate and waist circumference, from April to July, 2011. As a results, the mean score of SBP, DBP, TC, TG and BMI were significantly higher in smoking group than non-smoking group, but HDL-C were significantly lower in smoking group than non-smoking group, the distribution of hypertension was significantly higher in smoking group than non-smoking group. The abnormal levels of TC, TG and HDL-C were significantly higher in smoking group than non-smoking group, and the distribution of obesity was significantly higher in smoking group than non-smoking group. Age adjusted odds ratio(ORs) was significantly increase in smoking group than non-smoking group: hypertension(3.59 times), hypercholesteremia (1.49 times), hyperlipidemia(1.81 times), low HDL-cholesteremia(1.58 times), high level of atherogenic index(1.27 times). Above results suggested that the smoking related with blood pressure and serum lipid levels.

• Journal of Nutrition and Health
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• v.36 no.6
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• pp.635-645
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• 2003

This study was conducted to investigate the effects of smoking on nutrient intake and blood mineral status. The subjects were composed of two groups.55 smokers and 52 non-smokers. A 24-hour recall method was used along with questionnaires and serum mineral levels were analyzed by ICP spectrometer. The average ages of the smokers and non-smokers were 55.5 and 59.3 years old, respectively. The height, obesity degree, BMI, and WHR of the smokers were significantly higher than those of the non-smokers (p < 0.05, p < 0.05, p < 0.05, p < 0.01) Approximately 45.5% of the smokers smoked 16-20 cigarettes per day. The average age that the smokers started smoking was 22.0 years old and their smoking history was 33.5 years. About 74.5% of the smokers drank alcoholic beverages, while 44.2% of the non-smokers did. The smokers tended to eat less meals and breakfast meal, but drink coffee more often compared to the non-smokers. The mean daily energy intake and CPF energy intake ratio were 1231.8 ㎉ and 69.8 : 14.8 : 14.7 in the smokers and 1210.2 ㎉ and 72.1 : 14.7 12.7 in the non-smokers, respectively. The results show that the smokers tended to consume more energy, lipid, and cholesterol compared to the non-smokers. The results also show that in both groups, nutrient intake was lower than the RDA. The two groups were not significantly different in terms of the intake frequency of green-yellow vegetables and fresh fruits. There were no significant differences in serum levels of Ca, P, Mg, Cu, Fe, Mn, and Zn. However, serum Se level of the smokers was significantly higher than that of the non-smokers. In conclusion, the subjects of this study showed a serious imbalance in the nutrient intake, and the smokers showed a more undesirable dietary intake in the light of their high intake of energy, lipid, cholesterol, alcoholic beverages, and coffee. The serum Se level of the smokers was higher than that of the non-smokers, showing that Se is involved in smoking, Therefore, it could be suggested that more systematic research be conducted with respect to Se and smoking and that increased nutrition education and guidelines for smokers are required.

• Nutrition Research and Practice
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• v.10 no.4
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• pp.418-423
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• 2016

BACKGROUND/OBJECTIVES: Spirulina, a blue-green alga, is widely produced and commercialized as a dietary supplement with bio- and immune-modulatory functions. We have previously shown that spirulina had favorable effects on lipid profiles, immune functions, and antioxidant capacity in healthy Korean elderly. Despite favorable effect of spirulina supplementation, some sub-populations have shown a poor response to supplementation. Obesity is a factor related to poor-response. Therefore, the purpose of this study was to determine the immuno-modulation, antioxidant capacity, and lipid-lowering effect of spirulina in obese and non-obese Korean elderly. SUBJECTS/METHODS: The subjects were 78 elderly aged 60-87 years. In a randomized double blind, placebo-controlled study, subjects were fed either placebo or spirulina daily, at 8 g for 12 weeks. Subjects were divided into the non-obese group and the obese group based on body mass index (BMI) criteria for Asians suggested by the International Obesity Task Force: $BMI<25kg/m^2$ (non-obese) and $BMI{\geq}25kg/m^2$ (obese). RESULTS: In the non-obese group, spirulina supplementation showed a significant lowering effect on plasma concentration of total cholesterol and LDL-cholesterol, a significant increase in interleukin (IL)-2 concentration (P < 0.01) and a significant increment (P < 0.05) in IL-2/IL-6 ratio, and a significant increase in total antioxidant status level and a significant decrease in thiobarbituric acid reactive substances level. However, these effects were not observed in the obese group. CONCLUSION: These results demonstrated that blood lipid lowering and immune and antioxidant improving response for spirulina supplement was affected by obesity in Korean elderly.

• Journal of Pharmacopuncture
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• v.17 no.2
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• pp.34-40
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• 2014

Objectives: The objective of the present study was to investigate the lipid and the antioxidant regulatory potential of a multigrain diet in laboratory animals with reference to lipid profiles, tissue lipid peroxidation and antioxidant status. Methods: Two types of diets, with or without addition of cholesterol, were used in the study - a commercial diet and a formulated multigrain diet (with Sorghum vulgare, Avena sativa, Pennisetum typhoideum, Oryza sativa, Eleusine coracana and Zea mays grains). After a 10-week period of feeding the diets to albino rats the plasma, liver and fecal lipid profiles and the hepatic and renal antioxidant status of the animals that were fed the commercial and the formulated diets (with and without cholesterol addition) were assessed. Results: The commercial diet supplemented with cholesterol elevated the levels of plasma total lipids, total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein cholesterol (VLDL-C), as well as the atherogenic index (AI). The high-density lipoprotein cholesterol (HDL-C) content and the antioxidant profiles (total ascorbic acid, superoxide dismutase, catalase, glutathione peroxidase reduced glutathione) declined along with increases in lipid peroxidation. The formulated diet (with and without addition of cholesterol) was found to be more efficient than the commercial diet in controlling plasma, hepatic and fecal lipid profiles, as well as hepatic and renal lipid peroxidation and antioxidant status, than of the hypercholesteremic animals. Conclusion: The multigrain diet used in the present study is effective in countering the hyperlipidemia and oxidative stress caused by high cholesterol intake.

• Journal of Marine Bioscience and Biotechnology
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• v.6 no.2
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• pp.110-117
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• 2014

Recently microalgae have been proposed as a promising biodiesel feedstock, owing to their higher lipid productivity and non-arable land based cultivation system. Biomass and lipid productivities of microalgae are largely affected by various environmental and nutritional factors. In this study, the effects of nitrogen (nitrate and ammonium) and organic carbon (glucose and glycerol) sources on the cell growth and lipid production of Chlorella sp. KR-1 were examined in flask cultures. Under autotrophic culture conditions for 15 days, overall cell growth and lipid (fatty acid methyl ester, FAME) production with nitrate were better than those of ammonium, resulting in 1.06 g cell/L and 333 mg FAME/L, respectively. Maximal intracellular lipid contents (348 - 352 mg FAME/g cell) were observed at low concentrations of 1 mM for both nitrate and ammonium. In the supply of light, addition of glucose in the range of 1 - 20 g/L showed higher cell densities than the autotrophic cell growth condition. Higher lipid accumulation of 375 mg FAME/g cell could achieved at 5 g glucose/L albeit of relatively short incubation of 7 days. With glycerol, intracellular lipid contents were ~1.9 times lower than glucose cases although similar cell growths were observed for both carbon sources.

• Nutrition Research and Practice
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• v.1 no.1
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• pp.14-18
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• 2007

This study investigated the effect of physical training and oxidative stress on the anti oxidative activity and on plasma lipid profile. Forty eight rats were given either a physical training or no training for 4 weeks and were then subdivided into 3 groups: before-exercise (BE); during-exercise (DE); after-exercise (AE). The antioxidative activity was evaluated with the activities of catalase in plasma and superoxide dismutase (SOD), the ratio of reduced glutathione/ oxidized glutathione (GSH/GSSG) and the level of malondialdehyde (MDA) in liver. The plasma concentrations of triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C)) were also compared. Compared to those of non-training group. catalase activities of training group were lower before exercise but higher during and after exercise. SOD activities were higher regardless of exercise. GSH/GSSG ratio was higher before exercise but was not significantly different during exercise and even lower after exercise. There were no differences between non-training group and training group in MDA levels regardless of exercise. Compared to those of non-training group, atherosclerotic index of training group was lower after exercise and there were no significant differences before and during exercise. There were no differences between non-training group and training group in HDL-C regardless of exercise. These results suggest that moderate physical training can activate antioxidant defenses and decrease the atherosclerotic index and this beneficial effect is evident under exercise-induced oxidative stress.