• 한국임상수의학회지
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• 제27권5호
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• pp.501-507
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• 2010

본 연구는 국내 타일레리아에서 주요항원단백질(major surface protein) 유전자의 다양성을 분석해 보고자 수행되었다. 나아가 Msp 유전자의 다양성과 타일레리아의 병원성과의 관계도 분석하였다. 제주에 있는 목장으로부터 총 177마리의 한우 혈액을 공시재료로 사용하여 혈액검사와 18S rRNA를 표적으로 하는 PCR을 실행하였다. 그 후, 타일레리아 18S rRNA에 양성인 28마리 (16마리 빈혈군과 12마리의 정상군)를 무작위로 선발하여 Msp유전자의 염기 서열을 반복하여 분석하였다. 총 56개의 염기서열 결과는 다변성 부위(517-571 bp)에 따라 크게 type I에서 type V까지 5가지 형태로 나눌 수 있었는데, 이는 유전자은행(GenBank)에 등록되어 있는 다음의 유전자와 98.9% 이상 일치하였다 (Theileria spp. from China-EU584237; T. sergenti from China-DQ078264; Theileria spp. from Thailand-AB081329; Theileria spp. from Japan-AB218442; T. sergenti from Japan-AB016280). 그 분포는 22, 15, 9, 8, 2개가 각각 type I에서 V까지 분포하였고 빈혈과 관계없이 type I이 가장 많이 나타나는 것으로 밝혀졌다(37.5%의 빈혈군과 41.7%의 정상군). 나머지 type중에서는 type II가 빈혈군에서 가장 많이(37.5%) 나타났으며, 반면 type IV는 정상군에서 많이 (25%) 나타났다. 본 연구는 국내 타일레리아 Msp유전자의 다양성을 밝히는데 좋은 자료로 활용될 수 있을 것이다.

• 한국전통조경학회지
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• 제33권3호
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• pp.72-83
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• 2015

천연기념물 제1호인 도동 측백나무군락지에 대한 식물상 연구를 통하여 측백나무와 함께 지역 고유의 식생을 구성하는 식물종의 다양성을 발굴하고, 생태학적 특성을 분석하여, 국가식생/식물자원의 가치를 고찰하고자 하였다. 본 연구를 통하여 밝혀진 도동 측백나무림의 식물상은 67과 147속 199종 16변종, 4품종, 219분류군으로 확인되었다. 출현종 가운데 멸종위기종은 확인되지 않았으나, IUCN 기준의 취약종인 모감주나무와 약관심종인 측백나무, 가침박달의 분포가 확인되었다. 연구지역은 지질학적 연구에서 비석회암지역으로 분류되고 있으나, 측백나무를 포함한 부싯깃고사리, 금털고사리, 돌좀고사리, 산조팝나무, 분꽃나무, 청가시나무 등의 호석회식물이 다소 많은 수(15종)가 분포하고 있으며, 털개살구, 털조록싸리, 큰구와꼬리풀, 개나리(식재기원) 등 한국 특산식물도 4분류군이 분포하고 있어, 지역 종급원 형성에 중요한 서식처의 하나로 판단된다. 귀화식물은 큰닭의덩굴, 소리쟁이, 물냉이, 미국가막사리, 개망초 등의 분포가 확인되었으며, 귀화율과 도시화지수가 3.6%와 2.2%로 대구지역에 비해 매우 낮은 수치를 보였으며, 귀화식물에 의한 지역 생태계의 교란위협 정도는 높지 않은 것으로 판단된다. 본 연구지역은 측백나무가 고유의 식생경관을 구성하고 있다는 점뿐 아니라 국내에서 자생하는 측백나무림의 최남단분포지라는 것에서 큰 의미를 가지며, 서식처를 반영한 고유한 지역식물 구성종들이 독특한 종조성을 형성하고 있어 생태학적 보존가치가 높이 평가되었다. 나아가 측백나무 자생지의 서식처에 대한 질적 가치를 훼손하지 않는 보존방안의 필요성을 제기하였으며, 지속가능한 보전을 위한 방안들을 고찰하였다.

• 한국작물학회지
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• 제38권3호
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• pp.240-244
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• 1993

야콘의 재배기술의 확립을 위하여 멀칭효과 및 재식밀도 실험을 한 결과는 다음과 같다. 1. 지상부 생육은 7월 중순부터 직선적으로 증가하였으며, 밀칭구나 비멀칭구에서 재식밀도가 높을수록 초장의 생육이 양호하였고, 특히 70 ${\times}$ 55cm에서 가장 좋았다. 2. 측지수는 정식 후 3개월부터 발생하였으며, 멀칭 및 재식밀도에 따라 유의한 차이를 나타내지 않았다. 3. 야콘괴경의 생체수량은 멀칭구가 비멀칭구 보다 높았으며, 멀칭구에서는 70${\times}$40cm구가 3561.7kg으로 가장 높았다. 4. 괴근수는 멀칭구에서 밀식할수록 많았으며, 근경과 근장에서는 유의적인 차이가 없었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.37-37
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• 2019

Cryopreservation is one of the ideal and suitable methods for long-term storage of plant germplasm. The plant contaminated with diseases and pathogens are decreased the multiplication rate, survival rate and high quality of plants after cryopreservation. The aim of this work was to improve a micropropagation method for lily in Korea, which are cultivars and indigenous plant. In the last process of rinsing scales after surface-sterilization, we tried to control the diseases and pathogens lived within the tissue by rinsing in 0.03% sodium hypochlorite (NaClO) instead of sterile distilled water. Bulb scales of Lilium were cultured in vitro on MS medium supplemented with Plant Preservative Mixture (PPM). The newly small bulb formed from bulb-scales was transferred to MS medium. We checked the non-contamination and survival rate after 2 weeks in culture. Non-contamination was shown to be 70 to 90% in formed small bulbs. This study will help to mitigate microbial contamination in Lilium species micropropagation for cryopreservation.

• 한국군사과학기술학회지
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• 제16권3호
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• pp.260-267
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• 2013

A combat system for navy's battleship is a system of systems who supports naval indigenous operations by integrating and inter-operating many different kind of weapon and non-weapon systems, which has characteristics of large-scale complex computing system. This paper considers a characteristics of naval combat system which has been developed by domestic technology and suggests a way to improve future naval combat system in terms of computing architecture by applying commercial real-time operating system technologies. This paper also provides an evaluation criteria for combat system adaptability of real-time operating systems.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1087-1093
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• 2005

The population diversity and seasonal changes of bacterial communities in rice soils were monitored using both culture-dependent approaches and molecular methods. The rice field plot consisted of twelve subplots planted with two genetically-modified (GM) rice and two non-GM rice plants in three replicates. The DGGE analysis revealed that the bacterial community structures of the twelve subplot soils were quite similar to each other in a given month, indicating that there were no significant differences in the structure of the soil microbial populations between GM rice and non-GM rice during the experiment. However, the DGGE profiles of June soil after a sudden flooding were quite different from those of the other months. The June profiles exhibited a few intense DNA bands, compared with the others, indicating that flooding of rice field stimulated selective growth of some indigenous microorganisms. Phylogenetic analysis of l6S rDNA sequences from cultivated isolates showed that, while the isolates obtained from April soil before flooding were relatively evenly distributed among diverse genera such as Arthrobacter, Streptomyces, Terrabacter, and Bacillus/Paenibacillus, those from June soil after flooding mostly belonged to the Arthrobacter species. Phylogenetic analysis of 16S rDNA sequences obtained from the soil by cloning showed that April, August, and October had more diverse microorganisms than June. The results of this study indicated that flooding of rice fields gave a significant impact on the indigenous microbial community structure; however, the initial structure was gradually recovered over time after a sudden flooding.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.481-489
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• 2017

Tick-borne pathogens can cause serious problems in grazing cattle. However, little information is available on tick-mediated diseases in cattle grazing on mountains. Thus, this study aimed to understand the potential problems related to tick-borne diseases in grazing cattle through the investigation of prevalent tick-transmitted infections, and their associated hematological changes, in terms of season and grazing type in Korean indigenous cattle (=Hanwoo). Hanwoo cattle from 3 regions of the Republic of Korea (=Korea) were either maintained indoors or placed on grassy mountains from spring to fall of 2014 and 2015. Cattle that grazed in mountainous areas showed a greater prevalence of tick-borne infections with an increased Theileria orientalis infection rate (54.7%) compared to that in non-grazing cattle (16.3%) (P<0.001). Accordingly, the red blood cell (RBC) count and hematocrit (HCT) values of grazing cattle were significantly lower than those of non-grazing cattle throughout the season (P<0.05). Moreover, RBC, hemoglobin (Hb), and HCT of T. orientalis-positive group were significantly lower than those of T. orientalis-negative group (P<0.05). T. orientalis is a widespread tick-borne pathogen in Korea. Grazing of cattle in mountainous areas is closely associated with an increase in T. orientalis infection (RR=3.4, P<0.001), and with consequent decreases in RBC count and HCT. Thus, these findings suggest that the Hanwoo cattle in mountainous areas of Korea are at a high risk of infection by T. orientalis, which can lead to hematological alterations. This study highlights the necessity of preventive strategies that target T. orientalis infection.

• Asian-Australasian Journal of Animal Sciences
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• 제18권7호
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

• 대한환경공학회지
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• 제22권2호
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• pp.375-383
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• 2000

유일로 오염된 지역의 토양에서 toluene을 탄소원으로 이용하는 혼합미생물을 분리하여 toluene, benzene, ethylbenzene 및 xylene isomers(BTEX)의 분해특성을 관찰하였다. 단일기질 실험에서는 모든 BTEX의 분해가 이루어졌으며 toluene, benzene, ethylbenzene, p-xylene 순서로 분해되었다. BTEX 혼합기질 분해실험에서는 단일기질일 때보다 분해속도가 상대적으로 느려졌으며, ethylbenzene이 benzene보다 먼저 분해되는 것이 관찰되었다. 이중 혼합물질 반응 실험에서는 방해작용(inhibition), 촉진작용(stimulation), 그리고 비반응(non-interaction)과 같은 다양한 기질반응이 관찰되었으며, ethylbenzene은 benzene, toluene, xylene의 분해에 강한 방해영향을 주었다. Xylene 분해특성에서 m- 및 p-xylene은 혼합미생물에 탄소원으로 이용되었으며 benzene이나 toluene이 동시에 존재할 때는 xylene isomer의 분해가 촉진되었다. 그러나 o-xylene의 분해는 benzene에 의해서만 촉진되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권2호
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• pp.206-210
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• 2001

Six Holstein$\times$indigenous multiparous dairy cows, $60{\pm}8$ days in milk, were used in a double $3{\times}3$ Latin square design to investigate the efficiency of milk production. The dairy cows were randomly allocated to total mixed rations (TMR) containing ground corn, ground corn plus cassava chips 50:50 and cassava chips as main sources of total non-fiber carbohydrates. Ether extract and calculated energy intakes for dairy cows fed TMR containing cassava chips were lower (p<0.05) than those fed TMR containing ground corn and ground corn plus cassava chips 50:50. There were no differences (p>0.05) in daily DM intake (3.51, 3.41 and 3.29% BW), in 4% fat corrected milk (19.66, 20.59 and 20.23%), in milk protein (3.37, 3.27 and 3.33%), and in solids-not-fat (9.03, 8.90 and 8.99%) but there were differences in cost of diets per kg of 4% fat corrected milk (40.75, 34.33 and 28.17%; p<0.01) for dairy cows fed TMR containing ground corn, ground corn plus cassava chips 50:50, and cassava chips. It can be concluded that the efficiency of milk production (4% fat corrected milk per dry matter intake) for dairy cows fed TMR containing cassava was greater than for those fed TMR containing corn.