• 제목/요약/키워드: non-destructive sampling method

검색결과 19건 처리시간 0.022초

Molecular identification of selected parrot eggs using a non-destructive sampling method

  • Jung-Il Kim;Jong-Won Baek;Chang-Bae Kim
    • 환경생물
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    • 제41권2호
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    • pp.145-166
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    • 2023
  • Parrots have been threatened by global trade to meet their high demand as pets. Controlling parrot trade is essential because parrots play a vital role in the ecosystem. Accurate species identification is crucial for controlling parrot trade. Parrots have been traded as eggs due to their advantages of lower mortality rates and more accessible transport than live parrots. A molecular method is required to identify parrot eggs because it is difficult to perform identification using morphological features. In this study, DNAs were obtained from 43 unidentified parrot eggs using a non-destructive sampling method. Partial cytochrome b (CYTB) gene was then successfully amplified using polymerase chain reaction (PCR) and sequenced. Sequences newly obtained in the present study were compared to those available in the GenBank by database searching. In addition, phylogenetic analysis was conducted to identify species using available sequences in GenBank along with sequences reported in previous studies. Finally, the 43 parrot eggs were successfully identified as seven species belonging to two families and seven genera. This non-destructive sampling method for obtaining DNA and molecular identification might help control the trade of parrot eggs and prevent their illegal trade.

한국의 남해 거문도 암반조하대의 수심별 해조류의 분포: 조사기법에 따른 장·단점 (Vertical Distribution of Seaweeds in the Rocky Shore Ecosystem in Geomundo, South Coast of Korea: Analyzing Strengths and Weaknesses Using a Survey Method)

  • 배종일;황성일;한상조;성봉준;전병현
    • 한국수산과학회지
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    • 제57권3호
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    • pp.278-291
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    • 2024
  • This study performed three research methods (destructive, non-destructive, and panoramic image analysis) to determine the most useful for identifying seaweed habitat characteristics. Panoramic images are suitable for understanding the overall seaweed distribution. The composition of the main dominant seaweed species showed a significant difference between the destructive and non-destructive methods using Quadrat. The destructive method showed Rhodophyta dominance, whereas the non-destructive was showed Phaeophyta as dominant. Destructive methods are disadvantageous because they destroy natural communities in sea areas where bleaching is severe. Non-destructive methods are disadvantageous because of the difficulty in analyzing small species. Performing 10 surveys using a non-destructive method was found to explain up to 80% of the species identified by the destructive method. However, considering the difficulty of field investigation, repeating the investigation with non-destructive methods at least 6-7 times is necessary to ensure that more than 60% of the destructive methods can be interpreted. To understand the distribution of oceanic marine algae, performing both destructive and non-destructive surveys are necessary, as each has its own merits and demerits.

넙치(Paralichthys olivaceus) 근육 채취 방법에 따른 Kudoa septempunctata 진단 효율 비교 (Evaluation of a Non-destructive Diagnostic Test for Kudoa septempunctata in Farmed Olive Flounder Paralichthys olivaceus)

  • 송준영;정승희;최혜승
    • 한국수산과학회지
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    • 제51권1호
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    • pp.23-30
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    • 2018
  • Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.

부순모래 콘크리트의 비파괴 시험에 의한 압축강도 추정 (The Compressive Strength Prediction of Crushed Sand Concrete by Non-Destructive Test Method)

  • 김명식;장희석;백동일;신남균;김강민
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2006년도 춘계 학술발표회 논문집(II)
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    • pp.145-148
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    • 2006
  • Schmidt hammer and ultra-sonic method are commonly used for crushed sand concrete compressive strength test in a construction field. At present, various of equations for prediction of strength are present, which have been used in a construction field. The purpose of this study is to evaluate the correlation between prediction strength by presentation equations and destructive strength to test specimen, and find out which is a suitable equation for the construction site, In this study, a strength test was carried out destructive test by means of core sampling and traditional test. Non-destructive test was conducted Schmidt hammer and ultra-sonic method, the experimental parameter were concrete age, curing condition, test method and strength level. It is demonstrated that the correlation behavior of crushed sand concrete strength in this study good due to the perform analysis of correlation between core, destructive strength and non-destructive strength.

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破壤檢査詩의 最小費용 샘플링 檢査方式 (A minimum cost sampling inspection plan for destructive testing)

  • 趙星九;裵道善
    • Journal of the Korean Statistical Society
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    • 제7권1호
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    • pp.27-43
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    • 1978
  • This paper deals with the problem of obtaining a minimum cost acceptance sampling plan for destructive testing. The cost model is constructed under the assumption that the sampling procedure takes the following form; 1) lots rejected on the first sample are acreened with a non-destructive testing, 2) the screening is assumed to be imperfect, and therefore, after the screening, a second sample is taken to determine whether to accept the lot of to scrap it. The usual sampling procedures for destructive testing can be regarded as special cases of the above one. Utilizing Hald's Bayesian approach, procedures for finding the global optimal sampling plans are given. However, when the lot size is large, the global plan is very different to obtain even with the aid of an electronic computer. Therefore a method of finding suboptimal plan is suggested. An example with uniform prior is also given.

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콘코리트 성분분석을 위한 비파괴분석방법 (Non-destructive Inspection Methods for Componential Analysis of Concrete)

  • 김전상지;안태호;어본건인
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2006년도 추계 학술발표회 논문집
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    • pp.933-936
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    • 2006
  • Many non-destructive inspection methods have recently been developed for concrete structures. However, these methods can obtain only physical information of concrete, such as crack depth, delamination or position of reinforcement etc. near its surface. If chemical information is required, sampling and componential analyses may be earned out. Non-destructive method that can detect deterioration factors such as carbonation, chloride content or sulfate attack would be an outstanding innovation in inspection methodologies. In this research, near-infrared spectroscopy and X-ray fluorescence analysis were applied for componential analysis for concrete. These methods are very effective compared to traditional methods, therefore, working efficiency and maintenance cost will be improved.

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각종 비파괴 검사법에 의한 압축강도 상관연구 (A Study on The Compressive Strength Correlation by Various Nondestructive Test Method)

  • 최원호;신도철;이대우
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 1998년도 가을 학술발표대회 논문집(III)
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    • pp.767-772
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    • 1998
  • schumidt hammer and ultra-sonic method are commonly used for concrete compressive strength test in a construction field. At present, various kinds of equations for estimation of strength are present, which have been used in a construction field. The purpose of this study is to evaluate the correlation between estimation strength by presentation equations and destructive strength to test specimen, and find out which is a suitable equation for this construction site. In this study, a strength test was carried out destructive test by means of core sampling. Non destructive test was conducted Schumidt hammer and ultra-sonic method, the experimental parameter were concrete age, test method and strength level. It is demonstrated that the correlation behavior of concrete strength in this study good due to the performs analysis of correlation between core strength and nondestructive strength.

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부순모래 콘크리트의 비파괴 시험에 의한 압축강도 추정에 관한 연구 (A Study on the Compressive Strength Prediction of Crushed Sand Concrete by Non-Destructive Method)

  • 김명식;백동일;김강민
    • 콘크리트학회논문집
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    • 제19권1호
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    • pp.75-81
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    • 2007
  • 콘크리트는 전체 체적의 $70{\sim}80%$가 골재로 이루어져 있다. 따라서 골재의 품질은 콘크리트 특성에 지대한 영향을 미친다. 현재 건설 현장에서는 부순모래 콘크리트의 비파괴 강도 추정을 일반적으로 슈미트해머를 이용한 반발 경도법이나 초음파를 이용한 초음파속도법 그리고 둘을 조합한 복합법을 이용하고 있으며 기존의 천연골재를 사용한 콘크리트에 의해 얻어진 여러 가지 제안식들에 적용시켜 추정하고 있다. 따라서 본 연구의 목적은 전형적인 파괴 시험과 코어 채취 그리고 반발경도법과 초음파속도법을 이용하여 부순모래 콘크리트의 강도 추정식을 제안하고자 하였다. 실험의 변수는 양생 재령과 양생 조건 그리고 콘크리트의 설계기준강도이다. 그 결과 본 연구에서 얻은 제안식이 기존식에 비해 모든 측면에서 양호한 것으로 나타나 실제 건설 현장의 부순모래 콘크리트 구조물의 평가 기술 향상에 기여할 것으로 사려 된다.

Advanced Indentation Studies on the Effects of Hydrogen Attack on Tensile Property Degradation of Heat-Resistant Steel Heat-Affected Zones

  • Choi, Yeol;Jang, Jae-il;Lee, Yun-Hee;Kwon, Dongil;Kim, Jeong-Tae
    • Corrosion Science and Technology
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    • 제2권6호
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    • pp.266-271
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    • 2003
  • Safety diagnosis of various structural components and facilities is indispensable for preventing catastrophic failure of material by time-dependent and environment accelerating degradation. Also, this diagnosis of operating components should be done periodically for safe maintenance and economical repair. However, conventional standard methods for mechanical properties have the problems of bulky specimen, destructive procedure and complex procedure of specimen sampling. So, a non-destructive and simple mechanical testing method using small specimen is needed. Therefore, an advanced indentation technique was developed as a potential method for non-destructive testing of in-field structures. This technique measures indentation load-depth curve during indentation and analyzes the mechanical properties related to deformation such as yield strength, tensile strength and work-hardening index. In this paper, we characterized the tensile properties including yield and tensile strengths of the V-modified Cr-Mo steels in petro-chemical and thermo-electrical plants. And also, the effects of hydrogen-assisted degradation of the V-modified Cr-Mo steels were analyzed in terms of work-hardening index and yield ratio.

NONDESTRUCTIVE/IN-FIELD CHARACTERIZATION OF TENSILE PROPERTIES AND RESIDUAL STRESS OF WELDED STRUCTURES USING ADVANCED INDENTATION TECHNIQUE

  • Park, Yeol;Dongil Son;Kim, Kwang-Ho;Park, S. Joon;Jang, Jae-il;Dongil Kwon
    • 대한용접접합학회:학술대회논문집
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    • 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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    • pp.668-674
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    • 2002
  • Structural integrity assessment is indispensable for preventing catastrophic failure of industrial structures/components/facilities. This diagnosis of operating components should be done periodically for safe maintenance and economical repair. However, conventional standard methods for mechanical properties have the problems of bulky specimen, destructive and complex procedure of specimen sampling. Especially, the mechanical properties at welded zone including weldment and heat affected zone could not be evaluated individually due to their size requirement problem. So, an advanced indentation technique has been developed as a potential method for non-destructive testing of in-field structures. This technique measures indentation load-depth curve during indentation and analyzes the mechanical properties related to deformation such as yield strength, tensile strength and work-hardening index. Also indentation technique can evaluate a residual stress based on the concept that indentation load-depth curves were shifted with the direction and the magnitude of residual stress applied to materials. In this study, we characterized the tensile properties and welding residual stress of various industrial facilities through the new techniques, and the results are introduced and discussed.

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