• Title/Summary/Keyword: non-destructive sampling method

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Molecular identification of selected parrot eggs using a non-destructive sampling method

  • Jung-Il Kim;Jong-Won Baek;Chang-Bae Kim
    • Korean Journal of Environmental Biology
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    • v.41 no.2
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    • pp.145-166
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    • 2023
  • Parrots have been threatened by global trade to meet their high demand as pets. Controlling parrot trade is essential because parrots play a vital role in the ecosystem. Accurate species identification is crucial for controlling parrot trade. Parrots have been traded as eggs due to their advantages of lower mortality rates and more accessible transport than live parrots. A molecular method is required to identify parrot eggs because it is difficult to perform identification using morphological features. In this study, DNAs were obtained from 43 unidentified parrot eggs using a non-destructive sampling method. Partial cytochrome b (CYTB) gene was then successfully amplified using polymerase chain reaction (PCR) and sequenced. Sequences newly obtained in the present study were compared to those available in the GenBank by database searching. In addition, phylogenetic analysis was conducted to identify species using available sequences in GenBank along with sequences reported in previous studies. Finally, the 43 parrot eggs were successfully identified as seven species belonging to two families and seven genera. This non-destructive sampling method for obtaining DNA and molecular identification might help control the trade of parrot eggs and prevent their illegal trade.

Vertical Distribution of Seaweeds in the Rocky Shore Ecosystem in Geomundo, South Coast of Korea: Analyzing Strengths and Weaknesses Using a Survey Method (한국의 남해 거문도 암반조하대의 수심별 해조류의 분포: 조사기법에 따른 장·단점)

  • Jong Il Bai;Sung Il hwang;Sang Jo Han;Bong Jun Seong;Byeong Hyeon Jeon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.57 no.3
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    • pp.278-291
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    • 2024
  • This study performed three research methods (destructive, non-destructive, and panoramic image analysis) to determine the most useful for identifying seaweed habitat characteristics. Panoramic images are suitable for understanding the overall seaweed distribution. The composition of the main dominant seaweed species showed a significant difference between the destructive and non-destructive methods using Quadrat. The destructive method showed Rhodophyta dominance, whereas the non-destructive was showed Phaeophyta as dominant. Destructive methods are disadvantageous because they destroy natural communities in sea areas where bleaching is severe. Non-destructive methods are disadvantageous because of the difficulty in analyzing small species. Performing 10 surveys using a non-destructive method was found to explain up to 80% of the species identified by the destructive method. However, considering the difficulty of field investigation, repeating the investigation with non-destructive methods at least 6-7 times is necessary to ensure that more than 60% of the destructive methods can be interpreted. To understand the distribution of oceanic marine algae, performing both destructive and non-destructive surveys are necessary, as each has its own merits and demerits.

Evaluation of a Non-destructive Diagnostic Test for Kudoa septempunctata in Farmed Olive Flounder Paralichthys olivaceus (넙치(Paralichthys olivaceus) 근육 채취 방법에 따른 Kudoa septempunctata 진단 효율 비교)

  • Song, Jun-Young;Jung, Sung Hee;Choi, Hye-Sung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.1
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    • pp.23-30
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    • 2018
  • Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.

The Compressive Strength Prediction of Crushed Sand Concrete by Non-Destructive Test Method (부순모래 콘크리트의 비파괴 시험에 의한 압축강도 추정)

  • Kim, Myung-Sik;Jang, Hei-Suk;Beak, Dong-Il;Sin, Nam-Gyun;Kim, Kang-Min
    • Proceedings of the Korea Concrete Institute Conference
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    • 2006.05b
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    • pp.145-148
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    • 2006
  • Schmidt hammer and ultra-sonic method are commonly used for crushed sand concrete compressive strength test in a construction field. At present, various of equations for prediction of strength are present, which have been used in a construction field. The purpose of this study is to evaluate the correlation between prediction strength by presentation equations and destructive strength to test specimen, and find out which is a suitable equation for the construction site, In this study, a strength test was carried out destructive test by means of core sampling and traditional test. Non-destructive test was conducted Schmidt hammer and ultra-sonic method, the experimental parameter were concrete age, curing condition, test method and strength level. It is demonstrated that the correlation behavior of crushed sand concrete strength in this study good due to the perform analysis of correlation between core, destructive strength and non-destructive strength.

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A minimum cost sampling inspection plan for destructive testing (破壤檢査詩의 最小費용 샘플링 檢査方式)

  • 趙星九;裵道善
    • Journal of the Korean Statistical Society
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    • v.7 no.1
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    • pp.27-43
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    • 1978
  • This paper deals with the problem of obtaining a minimum cost acceptance sampling plan for destructive testing. The cost model is constructed under the assumption that the sampling procedure takes the following form; 1) lots rejected on the first sample are acreened with a non-destructive testing, 2) the screening is assumed to be imperfect, and therefore, after the screening, a second sample is taken to determine whether to accept the lot of to scrap it. The usual sampling procedures for destructive testing can be regarded as special cases of the above one. Utilizing Hald's Bayesian approach, procedures for finding the global optimal sampling plans are given. However, when the lot size is large, the global plan is very different to obtain even with the aid of an electronic computer. Therefore a method of finding suboptimal plan is suggested. An example with uniform prior is also given.

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Non-destructive Inspection Methods for Componential Analysis of Concrete (콘코리트 성분분석을 위한 비파괴분석방법)

  • Kanada, Hisashi;Ahn, Tae-Ho;Uomoto, Taketo
    • Proceedings of the Korea Concrete Institute Conference
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    • 2006.11a
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    • pp.933-936
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    • 2006
  • Many non-destructive inspection methods have recently been developed for concrete structures. However, these methods can obtain only physical information of concrete, such as crack depth, delamination or position of reinforcement etc. near its surface. If chemical information is required, sampling and componential analyses may be earned out. Non-destructive method that can detect deterioration factors such as carbonation, chloride content or sulfate attack would be an outstanding innovation in inspection methodologies. In this research, near-infrared spectroscopy and X-ray fluorescence analysis were applied for componential analysis for concrete. These methods are very effective compared to traditional methods, therefore, working efficiency and maintenance cost will be improved.

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A Study on The Compressive Strength Correlation by Various Nondestructive Test Method (각종 비파괴 검사법에 의한 압축강도 상관연구)

  • 최원호;신도철;이대우
    • Proceedings of the Korea Concrete Institute Conference
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    • 1998.10b
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    • pp.767-772
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    • 1998
  • schumidt hammer and ultra-sonic method are commonly used for concrete compressive strength test in a construction field. At present, various kinds of equations for estimation of strength are present, which have been used in a construction field. The purpose of this study is to evaluate the correlation between estimation strength by presentation equations and destructive strength to test specimen, and find out which is a suitable equation for this construction site. In this study, a strength test was carried out destructive test by means of core sampling. Non destructive test was conducted Schumidt hammer and ultra-sonic method, the experimental parameter were concrete age, test method and strength level. It is demonstrated that the correlation behavior of concrete strength in this study good due to the performs analysis of correlation between core strength and nondestructive strength.

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A Study on the Compressive Strength Prediction of Crushed Sand Concrete by Non-Destructive Method (부순모래 콘크리트의 비파괴 시험에 의한 압축강도 추정에 관한 연구)

  • Kim, Myung-Sik;Baek, Dong-Il;Kim, Kang-Min
    • Journal of the Korea Concrete Institute
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    • v.19 no.1
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    • pp.75-81
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    • 2007
  • Percentage that aggregate of materials that concrete composed about $70{\sim}80%$ of whole volume, therefore influence that quality of aggregate gets in concrete characteristics are very important. Schmidt hammer and ultra-sonic velocity method are commonly used for crushed sand concrete compressive strength test in a construction field. At present, various equations for prediction of strength are present, which have been used in a construction field. The purpose of this study is to evaluate the correlation between prediction strength by present equations and destructive strength to test specimen, and find out which is a suitable equation for the construction site, a strength test was carried out destructive test by means of core sampling and traditional test. The experimental parameters were concrete age, curing condition, and strength level. It is demonstrated that the correlation behavior of crushed sand concrete strength in this study good due to the perform analysis of correlation between core, destructive strength and non-destructive strength.

Advanced Indentation Studies on the Effects of Hydrogen Attack on Tensile Property Degradation of Heat-Resistant Steel Heat-Affected Zones

  • Choi, Yeol;Jang, Jae-il;Lee, Yun-Hee;Kwon, Dongil;Kim, Jeong-Tae
    • Corrosion Science and Technology
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    • v.2 no.6
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    • pp.266-271
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    • 2003
  • Safety diagnosis of various structural components and facilities is indispensable for preventing catastrophic failure of material by time-dependent and environment accelerating degradation. Also, this diagnosis of operating components should be done periodically for safe maintenance and economical repair. However, conventional standard methods for mechanical properties have the problems of bulky specimen, destructive procedure and complex procedure of specimen sampling. So, a non-destructive and simple mechanical testing method using small specimen is needed. Therefore, an advanced indentation technique was developed as a potential method for non-destructive testing of in-field structures. This technique measures indentation load-depth curve during indentation and analyzes the mechanical properties related to deformation such as yield strength, tensile strength and work-hardening index. In this paper, we characterized the tensile properties including yield and tensile strengths of the V-modified Cr-Mo steels in petro-chemical and thermo-electrical plants. And also, the effects of hydrogen-assisted degradation of the V-modified Cr-Mo steels were analyzed in terms of work-hardening index and yield ratio.

NONDESTRUCTIVE/IN-FIELD CHARACTERIZATION OF TENSILE PROPERTIES AND RESIDUAL STRESS OF WELDED STRUCTURES USING ADVANCED INDENTATION TECHNIQUE

  • Park, Yeol;Dongil Son;Kim, Kwang-Ho;Park, S. Joon;Jang, Jae-il;Dongil Kwon
    • Proceedings of the KWS Conference
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    • 2002.10a
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    • pp.668-674
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    • 2002
  • Structural integrity assessment is indispensable for preventing catastrophic failure of industrial structures/components/facilities. This diagnosis of operating components should be done periodically for safe maintenance and economical repair. However, conventional standard methods for mechanical properties have the problems of bulky specimen, destructive and complex procedure of specimen sampling. Especially, the mechanical properties at welded zone including weldment and heat affected zone could not be evaluated individually due to their size requirement problem. So, an advanced indentation technique has been developed as a potential method for non-destructive testing of in-field structures. This technique measures indentation load-depth curve during indentation and analyzes the mechanical properties related to deformation such as yield strength, tensile strength and work-hardening index. Also indentation technique can evaluate a residual stress based on the concept that indentation load-depth curves were shifted with the direction and the magnitude of residual stress applied to materials. In this study, we characterized the tensile properties and welding residual stress of various industrial facilities through the new techniques, and the results are introduced and discussed.

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