• 농업과학연구
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• 제48권4호
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• pp.875-890
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• 2021

In order to confirm the safety of a genetically modified organism (GMO), we assess its potential toxicity on non-target insects and spiders. In this study, the effects of GM soybean, a type of vitamin-A-enhanced transgenic soybean with tolerance to the herbicide glufosinate, were assessed under a field condition. The study compared this vitamin-A-enhanced transgenic soybean and a non-GM soybean (Gwangan) in a living modified organism (LMO) isolated field of Kyungpook National University (Gunwi) and the National Institute Agricultural Sciences (Jeonju) in the Republic of Korea in 2019 - 2020. In total, 207,760 individual insects and arachnids, representing 81 families and 13 orders, were collected during the study. From the two types of soybean fields, corresponding totals of 105,765 and 101,995 individuals from the vitamin-A-enhanced transgenic soybean and Gwangan samples areas were collected. An analysis of variance indicated no significant differences (p < 0.05). A multivariate analysis showed that the dominance and richness outcomes of plant-dwelling insects were similar. The data on insect species population densities were subjected to a principal component analysis (PCA) and an orthogonal partial least squares-discriminant analysis (OPLS-DA), which did not distinguish between the two varieties, i.e., the vitamin-A-enhanced transgenic soybean and the non-GM soybean in any cultivated field. However, the results of the PCA analysis could be divided overall into four groups based on the yearly survey areas. Therefore, there was no evidence for the different impact of vitamin A-enhanced transgenic soybean on the above-ground insects and spiders compared to non-GM soybean.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 추계학술대회
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• pp.132-132
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• 2020

아직까지 국내에서 GM작물이 상업화를 위해 승인된 예는 없지만 생명공학기술의 발전으로 GM작물의 개발은 급속한 증가 추세에 있다. 비의도적인 방출로 인해 미승인 LMO 목화가 전국적으로 재배되어 국립종자원 주관으로 양성 판정된 재배지의 목화를 폐기 처분하였으나(2017), GM작물이 유해하다는 인식과 환경에 방출되어 생태계를 교란시킨다는 인식이 팽배해 있는 현실에서 과학적으로 유전자의 이동성을 검증하는 노력이 중요하다. 자식성 작물의 화분의 이동성 조사를 위해 중앙의 코어 위치에 LM작물을 식재한 후 LM작물 주변에 재배품종을 심어 유전자이동 가능성을 조사하고 재배 환경에 의한 영향을 평가하기 위해 포장 주변 기상상황 데이터-온도, 습도, 풍속, 풍향, 기압, 강수량 등을 분석하고 기상상황이 화분의 전이에 미치는 영향 조사하였다.

• Weed & Turfgrass Science
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• 제3권3호
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• pp.221-224
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• 2014

Genetically modified (GM) crops are not permitted to be cultivated in Korea, but can only be imported as food or feed purposes. The import of GM crops has sharply increased in recent years, thus raising concerns with regard to the unintentional escape of these crops during transport and manufacturing as well as the subsequent contamination of local, non-GM plants. Hence, monitoring of GM crops was studied in or outside of grain receiving ports as well as from feed-processing plants in Korea during July 2008. We observed spilled maize grains and established plants primarily in storage facilities that are exposed around the harbors and near transportation routes of the feed-processing areas. Based on the PCR analyses, a total of 17 GM maize plants and 11 seeds were found among the samples. In most cases, the established maize plants found in this study were at the vegetative stage and thus failed to reach the reproductive stage. This study concludes that, in order to prevent a genetic admixture in the local environment for GM crops or seeds, frequent monitoring work and proper action should be taken.

• Journal of Plant Biotechnology
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• 제5권2호
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• pp.69-77
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• 2003

The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd. of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialization of these products are summarized. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.39-48
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• 2003

The ornamental industry encompasses cut flower, pot plant, turfgrass and nursery stock production and is an important part of the agricultural sector. As internationally traded commodities, cut flowers and plants are an integral part of the economy of a number of developing countries in South America, the Caribbean and Africa. Genetic modification (GM) is a tool with great potential to the ornamental horticulture industry. The rapid progress in our knowledge of plant molecular biology can accelerate the breeding ornamental plants using recombinant DNA technology techniques. Not only is there the possibility of creating new, novel products the driver of the industry but also the potential to develop varieties requiring less chemical and energy inputs. As an important non-food agricultural sector the use of genetically modified (GM) ornamental crops may also be ideal for the intensive farming necessary to generate pharmaceuticals and other useful products in GM plants. To date, there are only a few ornamental GM products in development and only one, a carnation genetically modified for flower colour, in the marketplace. International Flower Developments, a joint venture between Florigene Ltd. in Australia and Suntory Ltd.of Japan, developed the GM carnations. These flowers are currently on sale in USA, Japan and Australia. The research, development and commercialisation of these products are summarised. The long term prospects for ornamental GM products, like food crops, will be determined by the regulatory environment, and the acceptance of GM products in the marketplace. These critical factors will be analysed in the context of the current legislative environment, and likely public and industry opinion towards ornamental genetically modified organisms (GMO's).

• 한국식품저장유통학회:학술대회논문집
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• 한국식품저장유통학회 2007년도 학술발표회
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• pp.219.1-219.1
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• 2007

• 한국작물학회:학술대회논문집
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• 한국작물학회 2020년도 추계학술대회
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• pp.139-139
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• 2020

• 자동차안전학회지
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• 제6권2호
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• pp.67-71
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• 2014

Based on the analysis of the actual passenger vehicle fire cases for recent four years (2010~2013), the passenger vehicle fire is increasing annually. Main root cause was analyzed as an electric problem as a 39%. Vehicle fire case by electric problem was mainly caused by use of Non-genuine part. Vehicle fire case by mechanical problem was mainly caused by various oil system maintenance. Vehicle fire case by smoking material was mainly caused by cigarette and disposal lighter. And external fire transition issue and towing mistake fire cases was also confirmed.

• Pediatric Infection and Vaccine
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• 제5권1호
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• pp.147-151
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• 1998

On the treatment of Kawasaki disease, approximately 10% of children treated with IVIG have persistent or recrudescent fever despite IVIG treatment. We had experienced two children with Kawasaki disease who did not respond after multiple dosages of IVIG. They were treated within the first 10 days of onset of fever and were given oral aspirin (100mg/kg/day) and IVIG(2gm/kg) in a single infusion for 8 to 10 hours. The first child had not resolution of symptoms after three intravenous doses of IVIG(total 4gm/kg). And then treated with high dose methylprednisolone(30mg/kg) for 2 to 3 hours intravenously without symptoms improvement. On fifth hospital days, he was retreated with IVIG (2gm/kg) again with ultimate resolution of symptoms. The second child had resolution of symptoms after three intravenous doses of IVIG(total 4gm/kg). No adverse events were associated with the administration of IVIG or steroid. We reported two cases of IVIG non-responded Kawasaki disease with a brief review of the related literatures.

• KSBB Journal
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• 제18권6호
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• pp.435-439
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• 2003

형질 전환된 담배 seed에서 담배 식물체를 유도하여 White 액체 배지에서 기관 배양하였다. 암조건, sucrose 2%의 조건에서 좋은 growth pattern을 나타내었고, 계대 배양은 외마디법을 이용하여 2주마다 하였다. 기관 배양에서 hGM-CSF production pattern을 보면, intracellular에서는 큰 변화 없이 약 30 ng/g의 일정한 농도를 나타내었다. Extracellular에서 hGM-CSF 농도는 배양 6일 이후부터 급속하게 증가하기 시작하여 배양 12일째에 약 0.2ng/$m\ell$의 농도를 나타낸다. 기관배양은 다른 식물세포 배양 시스템에 비해 생산되어진 단백질의 안정성이 크다는 장점에 비해 세포 내에서 배지 내로의 단백질 분비가 적다는 단점이 있다. 이를 극복하기 위해 다양한 permeabilizing agents를 투여하여 담배 세포의 permeability를 증가시키고자 하였다. 그 결과, Pluronic F-68과 PEG8000을 첨가한 경우 담배 세포에서 배지 내로의 단백질 분비가 원활해졌음을 확인할 수 있었다.