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Quantitative Analysis of Coumarins from Angelica gigas Using $^1H$-NMR

  • Yoo, Jong-Su;Ahn, Eun-Mi;Song, Myoung-Chong;Bang, Myun-Ho;Kim, Dong-Hyun;Han, Min-Woo;Kwak, Ho-Young;Lee, Dae-Young;Lyu, Ha-Na;Baek, Nam-In
    • Food Science and Biotechnology
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    • v.17 no.3
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    • pp.573-577
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    • 2008
  • $^1H$-Nuclear magnetic resonance (NMR) spectrometry was applied to the quantitative analysis of coumarins in the roots of Angelica gigas without any chromatographic purification. The experiment was performed by the analysis of each singlet germinal methyl, which was well separated in the range of 1.0-2.0 ppm in the $^1H$-NMR spectrum. The quantity of the compounds was calculated by the ratio of the intensity of each compound to the known amount of internal standard (dimethyl terephthalate). These results were compared with the conventional gas chromatography (GC) method. The contents of decursin and decursinol angelate in A. gigas were determined $1.98{\pm}0.07$, $1.13{\pm}0.08%$ in quantitative $^1H$-NMR method and $2.06{\pm}0.24$, $1.17{\pm}0.24%$ in GC method, respectively. The advantages of quantitative $^1H$-NMR analysis are that can be analyzed to identify and quantify, and no reference compounds required for calibration curves. Besides, it allows rapid and simple quantification for coumarins with an analysis time for only 10 min without any preprocessing.

Application of Numerical Analysis for Sand Drain by the Multi-purpose Program of Soft Foundation Analysis (연약식반교양공법에 이용될 범용프로그램의 Sand Drain 공법에의 적용)

  • 박병기;정진섭
    • Geotechnical Engineering
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    • v.1 no.2
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    • pp.17-26
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    • 1985
  • This study was carried out for the purpose of comparing in reference to sand drain in the next three different cases. First, The case of drain material (sand pile) has some rigidity during embankment and consolidation. Second, In usual case of no rigidity as a paper drain without permeability during embankment and consolidation Third, Check up clay behavior when above the two cases carried out respectively. This FEM analysis is consisted with Biot's consolidation equation when it is used for Christian Boehmer's numerical technique. The main results are obtained from above the Analysis When sand drain has some rigidity, the lateral and vertical deformation of clay foundation is restrained considerable amount and .exhibited bearing capacity of load as a pile According to the foundation in drained condition and untrained condition, the results are much variable in this analysis method. Also, The behaviors of stress path and pore water pressure met our expectation during , consolidation. This analysis should be considered to put into use of sand drain and design in future.

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Prediction of the alveolar bone level after the extraction of maxillary anterior teeth with severe periodontitis

  • Hong, Chul Eui;Lee, Ju-Youn;Choi, Jeomil;Joo, Ji-Young
    • Journal of Periodontal and Implant Science
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    • v.45 no.6
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    • pp.216-222
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    • 2015
  • Purpose: After extraction, the alveolar bone tends to undergo atrophy in three-dimensions. The amount of alveolar bone loss in the horizontal dimension has been reported to be greater than the amount of bone loss in the vertical dimension, and is most pronounced in the buccal aspect. The aim of this study was to monitor the predictive alveolar bone level following the extraction of anterior teeth seriously involved with advanced chronic periodontitis. Methods: This study included 25 patients with advanced chronic periodontitis, whose maxillary anterior teeth had been extracted due to extensive attachment loss more than one year before the study. Periapical radiographs were analyzed to assess the vertical level of alveolar bone surrounding the edentulous area. An imaginary line connecting the mesial and the distal ends of the alveolar crest facing the adjacent tooth was arbitrarily created. Several representative coordinates were established in the horizontal direction, and the vertical distance from the imaginary line to the alveolar crest was measured at each coordinate for each patient using image analysis software. Regression functions predicting the vertical level of the alveolar bone in the maxillary anterior edentulous area were identified for each patient. Results: The regression functions demonstrated a tendency to converge to parabolic shapes. The predicted maximum distance between the imaginary line and the alveolar bone calculated using the regression function was $1.43{\pm}0.65mm$. No significant differences were found between the expected and actual maximum distances. Likewise, the predicted and actual maximum horizontal distances did not show any significant differences. The distance from the alveolar bone crest to the imaginary lines was not influenced by the mesio-distal spans of the edentulous area. Conclusions: After extraction, the vertical level of the alveolar ridge increased to become closer to the reference line connecting the mesial and distal alveolar crests.

Eating Quality Traits of Hanwoo longissimus dorsi Muscle as a Function of End-Point Cooking Temperature

  • Yang, Jieun;Jeong, Dawoon;Na, Chong-Sam;Hwang, Inho
    • Food Science of Animal Resources
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    • v.36 no.3
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    • pp.291-299
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    • 2016
  • Interaction between carcass quality grade and end-point cooking temperature on eating quality of Hanwoo m. longissimus was investigated. Ten (10) of steers were sampled from a commercial population; carcasses with QG 1++ (n=5) and QG 1 (n=5) were chosen. Samples were cooked by electric oven at 60 or 82℃ and compared with uncooked control samples. The pH was not affected by cooking temperature but decreased the redness after cooking and steaks cooked at 60℃ were more reddish than steaks cooked at 82℃ in both QG groups. Higher cooking temperature greatly (p<0.05) increased the cooking loss, but there was no significant interaction between cooking temperature and QG on the cooking loss. Moisture is negatively correlated with temperature in both QG while the proportionate relationship between crude fat and end-point temperature found in QG 1++. WBSF values were significantly (p<0.05) high for QG 1, while that was significantly (p<0.05) increased when the temperature continues to increase. The increasing quality grade of beef resulted in significant higher (p<0.01) level of TBARS and cooking temperature increased TBARS content. Fatty acid composition was not altered by cooking at both temperatures and also the amount of fat intake was not changed. The current study indicates that eating quality of beef m. longissimus was greatly influenced by end-point temperature being interacted with QG. However, the amount and composition of fat were stable regardless of end-point temperatures. These results will provide a consumer reference to determine cooking conditions and intramuscular fat content.

Evaluation of Conservation Conditions of Nara National Archives Documents (나라기록관 종이기록물의 보존 상태 평가)

  • Lee, Sang Kyu;Shin, Kwang Hyun;Kim, Hojin;Shin, Hyun Chang
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.46 no.2
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    • pp.22-29
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    • 2014
  • Many government agencies have used domestic deacidification equipments to stabilize archives; however, no agency has verified archives' current preservative status. We studied the Nara National Archives' documents that had treated by mass deacidification process from 2001 to 2012, in order to examine the documents' preservative status and how efficient the mass deacidification has been. Approximately 1% of total 112,970 Nara National Archives' documents treated by mass deacidification were randomly selected and evaluated on their current measure of acidity and color change. Regardless of the kinds of document material, a wide range of pH level was found. Especially those documents that were treated by mass deacidification equipment after 2006 showed acidic conditions that were less than pH 6.8 level. Those documents were probably not treated properly by deacidifying chemical because of tightly packing style. The current measures of acidity and color records will be useful as a reference for future evaluations of further decay of the documents. It was the first study to evaluate acidity and color conditions of Nara National Archives' documents treated by mass deacidification equipment. We concluded that when using mass deacidification equipment, archives should not be loaded tightly in order for them to be fully in contact with deacidifying chemical.

Blood Heavy Metal Concentrations of Korean Adults by Seafood Consumption Frequency: Using the Fourth Korea National Health and Nutrition Examination Survey (KNHANES IV), 2008 (국민건강영양조사 제4기 2차년도 (2008)를 이용한 어패류 섭취빈도에 따른 한국 성인의 혈중 중금속 농도 조사)

  • Kim, Young-A;Kim, Young-Nam;Cho, Kyung-Dong;Kim, Mi-Young;Kim, Eun-Jin;Baek, Ok-Hee;Lee, Bog-Hieu
    • Journal of Nutrition and Health
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    • v.44 no.6
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    • pp.518-526
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    • 2011
  • To determine blood heavy metal concentrations by seafood consumption in Korean adults, blood cadmium, mercury, and lead concentrations in a representative sample of 1,709 Koreans participated in the Fourth Korea National Health and Nutrition Examination Survey (KHANES IV-2) in 2008 were analyzed by age and seafood consumption frequency. The mean blood cadmium, mercury, and lead concentrations were $1.14{\pm}0.73{\mu}g/L$, $5.50{\pm}3.83{\mu}g/L$, and $2.56{\pm}1.22{\mu}g/dL$, respectively. The subjects aged ${\geq}$ 50 years had significantly higher blood cadmium concentrations than the subjects aged 20~39 years. Blood mercury concentrations of the 50's were significantly higher than those of the 20's and 30's (p < 0.05). Approximately, 43% of males and 22% of females had blood mercury concentrations > $5.8{\mu}g/L$ which is a blood mercury level equivalent to the current Reference Dose. Only 2 subjects had lead concentrations > $10{\mu}g/dL$, the standard lead level by the Centers for Disease Control and Prevention, USA. The subjects consuming pollack, mackerel, anchovy, corvina, shellfish, and salted seafood at least once a week had significantly higher mercury concentrations than the subjects consuming those seafoods less than once a month. However, there were no clear relationships between blood cadmium and lead concentrations and seafood consumption frequencies.

Effect of ${\beta}$-glucan Originated from Aureobasidium on the Dermal Wound Repair in Vitro Model (생체외 모델에서 아우레오바지디움 유래 베타 글루칸이 피부창상 치유에 미치는 영향)

  • Lee, Jung-Woo;Kwon, Young-Sam;Jang, Kwang-Ho
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.403-408
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    • 2013
  • The objective of the present study is to detect the effect of ${\beta}$-glucan originated from Aureobasidium on the proliferation and collagen production in human dermal fibroblast cells with wound repopulation in vitro. The proliferative effects were assessed using a MTT assay as well as cell counts at 24 and 48 hr after treatment. Hydroxyproline was measured as an index of procollagen production with reverse-phase high pressure liquid chromatography. Oncostatin M was used as a reference agent. In glucagon treated group, dose-dependent and significant increase of optical density or fibroblast cell numbers was demonstrated, when compared with those of control from 0.1 mg/ml concentration. In addition, the numbers of cells which had migrated into the wound defects were more significantly and dose-dependently increased than those of non-treated control. However, no meaningful effects on the procollagen production were observed.

Analysis of Patients Visiting an Oriental Hospital with Idiopathic Chronic Fatigue or Chronic Fatigue Syndrome (한방병원에 내원한 만성피로증후군과 특발성만성피로 환자에 대한 분석연구)

  • Son, Chang-Gue
    • The Journal of Korean Medicine
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    • v.34 no.3
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    • pp.119-125
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    • 2013
  • Objectives: Medically unexplained fatigue includes chronic fatigue syndrome (CFS) and idiopathic chronic fatigue (ICF). These disorders quite impair quality of life while no effective therapies exist. Therefore, patients with CFS or ICF frequently choose treatments based on traditional Korean medicine. The study aimed to analyze characteristic of patients visiting an Oriental clinic with CFS or ICF. Methods: Patients who met criteria for CFS or ICF were enrolled in this study, from March 2011 to Jun 2013. Clinical information focused on symptom differentiation and Sasang constitution was analyzed. Pearson's chi-square test was used to compare the frequency among sub-groups. Results: In total, 77 patients (49 male and 28 female) were diagnosed with CFS (17 male and 13 female) or ICF (32 male and 15 female). The symptom differentiation was 37.7%, 26.0%, 13.0%, and 23.3% for "Qi deficiency of both spleen and lung (脾肺氣虛)", "Yin deficiency of both liver and kidney (肝腎陰虛)", "Blood deficiency of both heart and spleen (心脾血虛)" and "Yang deficiency of both spleen and kidney (脾腎陽虛)", while Sasang constitutional distribution was 59.7%, 22.1% and 18.2% Soeumin, Taeumin and Soyangin, respectively. The fatigue severity was notably different between CFS ($63.7{\pm}12.1$) or ICF ($52.2{\pm}15.3$) according to the Chalder fatigue scale (10-point scale for eleven questions). There was statistical significance regarding symptom differentiation and Sasang constitution (p < 0.05). Conclusions: In the process of traditional Korean medicine-based development of therapeutics for patients with chronic fatigue or related conditions, this report can serve as reference data.

Evaluation of Near/Far Field and Directivity of Ultrasonic Transducer for Turbine Rotor Disc (터빈 로타 디스크의 초음파탐상을 위한 초음파탐촉자의 지향성 및 탐상범위)

  • Won, S.H.;Chang, H.K.;Cho, K.S.;Lee, J.O.;Lee, J.K.
    • Journal of the Korean Society for Nondestructive Testing
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    • v.18 no.3
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    • pp.163-171
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    • 1998
  • Near/far field length and directivity of transducers were investigated for the improvement and evaluation of the detectability of flaws in a disc. The reference block is fabricated for the disc of stage 6 in Yonggwang unit 1. The near/far field and directivity of an ultrasonic transducer with the center frequency of 5MHz were calculated for the inspection of the disc. These values showed good agreements with the experimental results. In the system composed of a wedge and a disc, those are evaluated theoretically and experimentally for the specimen with the artificial flaws of the size 2mm and 4mm and an ultrasonic transducer with the center frequency 5MHz and diameter 0.5inch. The detectability of keyway-flaw and detectable region for inspection were evaluated by using both tangential $45^{\circ}$ and $90^{\circ}$ transducers located at the distance of 53mm and 75mm from the disc hub, respectively.

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Development and Effectiveness of the Critical Pathway for Laparoscopic Cholecystectomy Patients (복강경 담낭 절제술 환자의 표준관리지침 개발 및 적용 효과)

  • Jun, Seong-Sook;Park, Young-Ju
    • Journal of Korean Academy of Nursing Administration
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    • v.8 no.1
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    • pp.123-135
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    • 2002
  • Purpose: In this study, the critical pathway for laparoscopic cholecystectomy patients was developed and applied for clinical study. The effectiveness of the critical pathway was analyzed. Method: The subjects with no critical pathway services of this study were 30 laparoscopic cholecystectomy operation patients, who were hospitalized in B General Hospital in Busan from Nov. 28. 2000 to May 2. 2001. The subjects with critical pathway services of this study were 30 laparoscopic cholecystectomy operation patients, who were hospitalized in B General Hospital in Busan from June. 11. 2001 to Oct. 31. 2001. Results: 1. Development for critical pathway Preliminary critical pathway for the laparoscopic cholecystectomy patients was developed though analysis of the reference and 34 case of medical record. The items on the y-axis were assessment, test, treatment, diet, fluid, consult, medication, activity and education and the items on the x-axis were till 3days after operation. The developed critical pathway was applied in clinical field. 2. Effectiveness of application on developed critical pathwayPost operational complication was not found in neither the critical pathway using Group nor Non-using Group. Hospitalization period mean was 4.63 days for the using Group and 5.93 days for the Non-using Group(P=.001). The mean cost for medical examination and treatment for the using Group was 786,270 won, 117,454 won(12.9%) less than that of the Non-using Group, 903,724 won(P=.000). The degree of satisfaction for the using group was 3.7 points, for the Non-using Group, 3.15 points. The degree of satisfaction for the used Group was 0.55 points higher than that for the Non-using Group and there was a statistically significant difference(P=.000). Concousion: From results of this study, Using the critical pathway in the laparoscopic cholecystectomy operational patients was effective in decreasing the hospitalization period and cost, and increasing the satisfaction about the medical service.

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