• Preventive Nutrition and Food Science
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• 제11권4호
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• pp.273-277
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• 2006

Recently, Rhei Rhizoma extracts (RRE) have begun to receive more attention as potential biological response modifiers. In the present study, we studied the antiproliferative effect of RRE on tumor cells and the effect of RRE on macrophage function. A variety of tumor cells and macrophages were treated with RRE at various concentrations. The effect of RRE on cell proliferation was measured by MTT assay and the effect of RRE on the production of nitric oxide (NO) was determined in the macrophage-like cell lines Raw264.7, C6 and peritoneal macrophages (pMQ). RRE inhibited the growth of tumor cells (e.g., B16, HOS). However, the effects of RRE on the production of NO varied with macrophage types. RRE had no effect on C6 cell growth and slightly increased the growth of Raw264.7 cells. In addition, treatment of normal pMQ with RRE enhanced NO production in a concentration-dependent manner, whereas RRE suppressed NO production at $50\;{\mu}g/mL$ in both Raw264.7 and C6 cells. However, RRE suppressed NO production in LPS/IFN-$\gamma$-stimulated C6 cells. Overall, these results suggest that RRE elicits an antiproliferative property and differentially modulates NO production in various macrophages, and have a potential for therapeutic application.

• The Plant Pathology Journal
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• 제33권1호
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• pp.95-100
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• 2017

Fungicide-resistant Alternaria alternata impede the practical control of the Alternaria diseases in crop fields. This study aimed to investigate cytological fungicide resistance mechanisms of A. alternata against dicarboximide fungicide iprodione. A. alternata isolated from cactus brown spot was cultured on potato-dextrose agar (PDA) with or without iprodione, and the fungal cultures with different growth characteristics from no, initial and full growth were observed by light and electron microscopy. Mycelia began to grow from one day after incubation (DAI) and continued to be in full growth (control-growth, Con-G) on PDA without fungicide, while on PDA with iprodione, no fungal growth (iprodione-no growth, Ipr-N) occurred for the first 3 DAI, but once the initial growth (iprodione-initial growth, Ipr-I) began at 4-5 DAI, the colonies grew and expanded continuously to be in full growth (iprodione-growth, Ipr-G), suggesting Ipr-I may be a turning moment of the morphogenetic changes resisting fungicidal toxicity. Con-G formed multicellular conidia with cell walls and septa and intact dense cytoplasm. In Ipr-N, fungal sporulation was inhibited by forming mostly undeveloped unicellular conidia with degraded and necrotic cytoplasm. However, in Ipr-I, conspicuous cellular changes occurred during sporulation by forming multicellular conidia with double layered (thickened) cell walls and accumulation of proliferated lipid bodies in the conidial cytoplasm, which may inhibit the penetration of the fungicide into conidial cells, reducing fungicide-associated toxicity, and may be utilized as energy and nutritional sources, respectively, for the further fungal growth to form mature colonies as in Ipr-G that formed multicellular conidia with cell walls and intact cytoplasm with lipid bodies as in Con-G.

• 한국유기농업학회지
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• 제15권4호
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• pp.437-452
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• 2007

친환경자재인 키토산과 목초액 처리가 가지 및 잎상추의 생육에 미치는 효과를 검토한 결과 다음과 같은 몇 가지 결론을 얻었다. 1. 키토산과 목초액 처리가 가지의 생육에 미치는 영향 1) 키토산과 목초액 처리 전 후 토양의 성분변화는 큰 차이는 없었으며, 총 미생물 수가 증가한 경향을 보였고, 미생물 종류 중에는 세균수가 많이 증가하였다. 그리고 방선균, 사상균 및 트리코데마수는 처리간 일정한 경향은 없었다. 2) 가지의 생육 및 수량은 대조구에 비해 키토산과 목초액 처리구에서 공히 유의하게 높았으며, 처리간에는 유의적인 차이는 없었다. 가지의 10a당 수량은 키토산과 목초액 처리구 공히 대조구에 비해 수량이 증가하였고, 처리 간에는 유의적인 차이는 없었다. 2. 키토산과 목초액 처리가 잎상추의 생육에 미치는 영향 1) 키토산과 목초액 처리 전 후 토양의 성분변화는 큰 차이는 없었으며, 미생물상에서는 총 미생물수가 증가하였고, 미생물 종류 중에는 세균수가 많이 증가하였다. 그 외 방선균, 사상균 및 트리코데마수는 처리간 일정한 경향은 없었다. 2) 잎상추의 생육은 대조구에 비해 키토산과 목초액 처리 공히 유의적인 차이가 인정되었으며, 처리간에는 유의적인 차이는 인정되지 않았다. 대체적으로 처리농도가 높을수록 생육이 양호한 것으로 나타났다.

• 상하수도학회지
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• 제26권6호
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• pp.833-840
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• 2012

This study aimed to investigate growth rate and nutrient removal efficiency of Chlorella vulgaris according to nitrogen sources and frequency of pH adjustment. Nitrogen and phosphorus removal efficiencies were evaluated in the three different conditions using $NO_3{^-}$, $NH_4{^+}$ as a sole nitrogen source and mixed condition. Initial nutrient concentrations in artificial wastewater were 30 mg-N/L and 3 mg-P/L similar to secondary wastewater effluent. When nitrogen source was $NO_3{^-}$, there was no inhibition on the growth of C. vulgaris with adjusting pH every 24 hr while growth inhibition occurred with $NH_4{^+}$ caused by pH drop. N, P removal efficiencies were no significant depending on the nitrogen sources. As pH was adjusted to 7 by pH-stat, growth rate and nutrient removal efficiencies were increased compared to adjusting pH every 24 hr, however, growth rate and nutrient removal efficiencies were no significant depending on the nitrogen sources.

• Journal of Plant Biology
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• 제24권1호
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• pp.13-19
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• 1981

Ammonium citrate와 ammonium succinate가 고려인삼(Panax ginseng C. A. Meyer) 배양세포의 생육 및 질소성분의 흡수에 미치는 영향을 조사하기 위하여 Murashige and Skoog 배지에서 ammonium nitrate 대신에 농도를 달리하여 ammonium citrate와 ammonium succinate를 첨가한 MS배지에 인삼 callus를 액체 진탕배양하여 다음과 같은 결론을 얻었다. Ammonium citrate 10mM과 ammonium succinate 10mM을 첨가한 배양기에서 생육량, NO$_3$-N 흡수량 및 전질소량이 control보다 많았고, NH$_4$-N 흡수량은 control과 같았다. 또한 각각 5mM씩 첨가한 경우에는 거의 모두 control과 별 차이가 없었다. 결론적으로 ammonium citrate 10mM과 ammonium succinate 10mM첨가한 배양기에서는 NO$_3$-N 등의 흡수를 촉진하여 배양세포의 대사를 촉진시켜 생육량을 증가시키는 것으로 생각된다. 그리하여 ammonium citrate 또는 ammonium succinate의 농도를 조절 공급하면 인삼 배양세포의 증식을 촉진시킬 수 있다고 사료된다.

• The Journal of Asian Finance, Economics and Business
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• 제8권8호
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• pp.325-334
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• 2021

The study investigates the effectiveness of the macroprudential policy on credit growth in Vietnam. The authors use the logic of the transmission mechanism of macroprudential policy on credit growth. Research variables include economic growth, inflation, interest rate, and quarterly bank-level data from 28 commercial banks in Vietnam during 2011-2018. The results reveal that: (i) GDP growth had a positive impact on credit growth of small banks but had no impact on large banks, (ii) Domestic Systemically Important Banks (D-SIBs) and small banks respond differently to macroprudential measures of imposing different credit growth targets for different bank groups, (iii) Restrictions on foreign currency loans are found to be effective in curbing credit growth for the full sample and small banks, (iv) Inflation and economic cycle have significantly impacted credit growth at bank-level in Vietnam and (v) Interestingly, a significant positive relationship between interest rates and credit growth is found for the full sample and D-SIBs in Vietnam. The findings suggest that a stable macroeconomic environment should be good conditions for financial stability, and monetary authority should pay more attention to small banks' behaviors than D-SIBs behavior, toward such "administration" tools since small banks tend to prefer "breaking the rules" to make profits.

• 원예과학기술지
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• 제33권3호
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• pp.356-363
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• 2015

This study examined the effects of different day/night temperature regimes or silver ion on growth and flowering of passion fruit 'Tai-nung No.1'. Low temperature treatment ($20/15^{\circ}C$) caused passion fruit cultivar 'Tai-nung No.1' to fail to flower. Flowering induction occurred within a temperature range of $20-30^{\circ}C$, with no significant difference in the days to first flower bud and the total number of flower buds between plants grown at $30/25^{\circ}C$ and $25/20^{\circ}C$. However, plants grown at $30/25^{\circ}C$ exhibited their first flower buds set on the higher nodes and had higher abortion rates of flower buds than those at $25/20^{\circ}C$. Plants grown at $30/25^{\circ}C$ had the most rapid growth and the shortest plastochron. We also evaluated the effect of the ethylene response inhibitors silver nitrate ($AgNO_3$) and silver thiosulfate (STS) on growth and flowering of potted passion fruit 'Tai-nung No.1', when they were exposed to low temperature conditions ($20/15^{\circ}C$) following chemical treatments ($AgNO_3$ or STS, at 0.5 or 1.0 mM). $AgNO_3$ and STS treatments induced flower formation and initial flower bud formation within approximately two weeks at $20/15^{\circ}C$ whereas non-treated control plants exhibited no flower formation. ACC content and activity of ACC oxidase in the leaves of passion fruit 'Tai-nung No.1'exposed to low temperature conditions ($20/15^{\circ}C$) were significantly inhibited by the ethylene inhibitor treatments. These results indicate that ethylene, which is produced under low temperature conditions, plays an important role in inhibiting flower formation in passion fruit.

• 생명과학회지
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• 제7권2호
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• pp.107-111
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• 1997

양어장에서 어류의 배설물 및 어류에 의해 소비되지 않고 있는 사료에 의한 암모니아성 질소이 제거를 위한 수처리 공법의 하나로써 질산화세균 및 탈질화 세균을 이용한 고정화 방법의 개발을 위해서는 우수한 암모니아 산화능력이 있는 미생물의 분리가 절대적이다. 이를 위하여 여러 곳으로부터 분리한 암모니아 산화 세균 가운데 생유깅 빠르고 $NO_{2}$ 생성이 뛰어난 Nitrosomonas sp. PK1을 선별하였다. 분리된 균의 생육에 따른 $NO_{2}$ 생성은 non-growth asso-ciated pattern을 보여주고 있었다. 또한 생육에 영향을 미치는 탄소원 가운데 glucose가 생유게 가장 좋았으나 농도가 0.1% 이상에서는 오히려 $NO_{2}$생성이 감소되었다. 따라서 고정화 방범에 이용하기 위해서는 생육과 $NO_{2}$생성 능력이 유지를 위한 적절한 생육 배지의 필요성을 보여주었다. Ion 첨가에 의한 영향을 검토한 결과 $ZnCl_{2}$의 첨가가 생육 및 $NO_{2}$ 생성량의 증가를 보여 주었으며 그 농도는 1~10mM로서 제한적임을 나타내었다.

• 한국결정성장학회지
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• 제18권3호
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• pp.122-124
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• 2008

The partial phase diagram of $NaNO_3$-NaCl system was investigated and the eutectic temperature was determined as $294.5^{\circ}C$. A typical rod eutectics of $NaNO_3$-4.56 wt%NaCl was directionally solidified. The results of interrod spacing, ${\lambda}_E$ as a function of growth velocity, V, were. obtained as ${\lambda}_E\;V^{0.39}\;=\;5.26$ (temperature gradient, $G_l\;=\;21.4^{\circ}C/mm$) and ${\lambda}_E\;V^{0.32}\;=\;5.45$ ($G_l\;=\;3.9^{\circ}C/mm$) and the exponent numbers of growth velocity were smaller than the theoretical value, 1/2. The sample rotation applied during directional solidification made the interrod spacing decrease slightly.

• 한국식품위생안전성학회지
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• 제9권1호
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• pp.31-36
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• 1994

The acute toxicity of recombinant human epidermal growth factor, DWP-401 was evaluated in SD rats. Male and female rats aging 6 weeks were administered orally or subcutaneously with 0, 0.125, 0.25, 0.5, 1 and 2 mg/kg of DWP-401. No deaths and no toxic symptoms related to the DWP-401 were observed. The body weights of treated animals were not significantly different from the controls. The results of necropsy revealed no abnormal gross findings of the organs in treated animals. LD50 values of DWP-401 for male and female rats were estimated to be over 2 mg/kg, which is approximately 2, 000 times of expected clinical dose.