This study has been aimed to determine the ecological factors with relationship to the Jongkok production in view of fermentation technology by means of some strains, Asp. kawachii, which is now preserved by the author and the following factors are included during the study; inorganic salts, nitrogen, sugar, water contents and temperature. The results, are as follows: (a) Sugar among other above-mentioned factors is increasingly affecting the number of the short type of conidiophore on culture medium and the conidiophore is increased by direct ratio until glucose concentration of 50%, at which concentration is mostly effective for the short type of conidiophore, while other factors did not affect on it. (b) Until glucose concentration of 50% sugar component of culture medium is favorable for the spore formation of Asp. kawachii by direct ratio. And peptone or asparagine on nitrogen medium, calcium-phosphate among other inorganic salts, wheat bran and rice branare also favorable, but other factors rientioned earlier show no relationship with the spore formation. Sugar, however, also related with the spore color clearness of crimson and light brown, and spore color is mostly clear at the point of glucose concentration until 50%. And asparagine on nitrogen medium, calcium phosphate among other inorganic salts, rice bran did all affect on the color clearness, while other factors did not concern with color clearness. (c) Water, sugar and temperature have related with the acid formation which is promoted, by direct ratio at the point of water-saturated condition and glucose concentration of 50%, while temperature at $25^{\circ}C$favorably affected on the acid formation which is increased by inverse ratio at the temperature$25^{\circ}C$ to $45^{\circ}C$ And pH did not relate with the acid formation. (d) Cylindrical plate method devised by the author is mostly favorable for the preservation and isolation of culture, compared with the traditional slant medium method.
Journal of the Korean Society of Marine Environment & Safety
/
v.29
no.4
/
pp.356-362
/
2023
The risk of climate change has been long acknowledged, and ongoing efforts to overcome this issue, within the shipping sector, with the international maritime organization playing a central role. Conducting research on characteristics of soot formation is crucial to control its occurrence within the combustion process. In this study, the laser extinction method and chemical reaction numerical analysis were employed to examine the alterations in the state of chemical species associated with flame temperature, flame visual, and soot formation by mixing nitrogen, an inert gas, in the counterflow diffusion flame based on ethylene gas. The findings of the study suggest that as the mixing ratio of nitrogen increased, both the flame temperature and soot volume fraction decreased. Additionally, the area in which soot particles were distributed also decreased, and the volume fraction decrease rate declined when the mixing ratio increased by more than 30%. The mole fraction of the chemical species involved in soot growth also decreased. the chemical species associated with the HACA reaction were affected by variations in the hydrocarbon fuel ratio, and the chemical species related to the odd carbon path were confirmed to be affected by the flame temperature as well as the hydrocarbon fuel ratio.
In order to investigate the effect of dietary zinc and phytic acid levels on protein metabolism in rats, male rats of Sprague-Dawley strains weighing approximately $60\~74g$ were fed different diets which contained 0, 0.35 and $1.05\%$ phytic acid each at 3 levels of zinc(0, 30 and 1,500 ppm zinc) for 28 days. Result obtained in this experiment are summarized as follows; 1. Body weight gait food consumption food efficiency ratio and protein efficiency ratio were lower in the rats fed zinc deficient diet(0 ppm zinc) than in those consuming 30 or 1,500 ppm dietary zinc, and the additional effect of phytic acid were not observed in all of then 2. Liver weight was lower in the rats fed 30 ppm zinc diet than in those fed 0 or 1,500 ppm-zinc diet but kidney and spleen weights were lower in the rats fed zinc deficient diet than in those fed 30 or 1,500 ppm-zinc diet Among organs measured only the liver appeared to be influenced by dietary phytic acid: the more the dietary phytic acid, the more the weight of liver, 3. Fecal nitrogen was decreased in the rats fed zinc deficient diet compared with those fed 30 or 1,500 ppm dietary zinc. Urinary nitrogen was increased in the rats fed $1.05\%$ dietary phytic acid compared with those fed 0.35 or $0\%$ dietary phytic acid Nitrogen retention of rat was influenced by neither dietary zinc nor phytic acid. 4. Urea nitrogen was decreased with increasing dietary zinc levels, and creatinine and uric acid levels were increased with increasing dietary zinc concentration or with additional quantity of phytic acid. Uric acid appeared to be influenced by zinc x phytic acid interaction; especially, the presence of phytic acid in the 30 ppm-zinc diet had significant effect on uric acid content. 5. Hemoglobin concentrations and hematocrit ratio were higher in the rats fed 30 ppm dietary zinc than in those fed 0 or 1,500 ppm-zinc diet Serum zinc concentration was increased with increasing dietary zinc levels. The content of total protein albumin and BUN and the ratio of albumin to globulin in serum, and protein content in liver were influenced by neither dietary zinc nor phytic acid.
Han, Sim-Hee;Kim, Du-Hyun;Kim, Gil Nam;Yun, Chung-Weon
Journal of Korean Society of Forest Science
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v.101
no.1
/
pp.62-68
/
2012
We have investigated needle life span, photosynthetic pigment and nitrogen allocation pattern in the needle of Picea jezoensis in the three populations (Gyebangsan, Deogyusan and Jirisan) to find out growth environmental condition which needs the strategy development of conservation and restoration against population decline. Needle survival rate was the highest in Gyebangsan (87.0%) and the lowest in Jirisan (71.6%), and it decreased with the increase of needle age. Needle chlorophyll a and b in Deogyusan and Jirisan showed higher content in older needle, but chlorophyll content in Gyebangsan was the highest in 2-year-old needle. Carotenoid content was the highest in the needle in Gyebangsan, and it increased along with needle age. Chlorophyll a/b ratio showed the highest value in Jirisan and the lowest value in Gyebangsan. Chlorophyll/carotenoid ratio decreased with needle age, Needle nitrogen content was the highest in Deogyusan (1.51%) and the lowest in Gyebangsan (1.40%), and the older needle had the lower content. In contrast, the highest chlorophyll/nitrogen ratio represented the oldest needle.
As a result of the cost of grains, the replacement of grains by co-products (i.e. DDGS) in feedlot diets is a common practice. This change produces diets that contain a lower amount of starch and greater amount of fibre. Hypothetically, combining feed grade urea (U) with slow release urea (Optigen) in this type of diet should elicit a better synchrony between starch (high-rate of digestion) and fibre (low-rate of digestion) promoting a better microbial protein synthesis and ruminal digestion with increasing the digestible energy of the diet. Four cannulated Holstein steers ($213{\pm}4$ kg) were used in a $4{\times}4$ Latin square design to examine the combination of Optigen and U in a finishing diet containing different starch:acid detergent fibre ratios (S:F) on the characteristics of digestive function. Three S:F ratios (3.0, 4.5, and 6.0) were tested using a combination of U (0.80%) and Optigen (1.0%). Additionally, a treatment of 4.5 S:F ratio with urea (0.80% in ration) as the sole source of non-protein nitrogen was used to compare the effect of urea combination at same S:F ratio. The S:F ratio of the diet was manipulated by replacing the corn grain by dried distillers grain with solubles and roughage. Urea combination did not affect ruminal pH. The S:F ratio did not affect ruminal pH at 0 and 2 h post-feeding but, at 4 and 6 h, the ruminal pH decreased as the S:F ratio increased (linear, p<0.05). Ruminal digestion of OM, starch and feed N were not affected by urea combination or S:F ratio. The urea combination did not affect ADF ruminal digestion. ADF ruminal digestion decreased linearly (p = 0.02) as the S:F ratio increased. Compared to the urea treatment (p<0.05) and within the urea combination treatment (quadratic, p<0.01), the flow of microbial nitrogen (MN) to the small intestine and ruminal microbial efficiency were greater for the urea combination at a S:F ratio of 4.5. Irrespective of the S:F ratio, the urea combination improved (2.8%, p = 0.02) postruminal N digestion. As S:F ratio increased, OM digestion increased, but ADF total tract digestion decreased. The combination of urea at 4.5 S:F improved (2%, p = 0.04) the digestible energy (DE) more than expected. Combining urea and Optigen resulted in positive effects on the MN flow and DE of the diet, but apparently these advantages are observed only when there is a certain proportion of starch:ADF in the diet.
Kim, Dami;Kim, Kil-Ja;Kim, Seon-Gon;Park, Hye-Sung
Journal of Mushroom
/
v.18
no.4
/
pp.311-316
/
2020
The effect of the nitrogen content of sawdust medium (1.2~1.8%) and the restriction stage temperature (2, 4, and 6℃) on the growth and storage characteristics of Flammulina velutipes (winter mushroom) were investigated. With increased nitrogen content, the growth days shortened and the yield of the fruiting body increased. The effect of restriction temperature on the growth of the fruiting body differed depending on the nitrogen content. No difference in restriction temperature was evident for T1 with a low nitrogen content of 1.28%. In medium with a nitrogen content ≥1.55%, the yield and length of the pileus and stipe increased as the restriction temperature decreased. The weight loss ratio according to the storage period did not change according to the nitrogen content in the medium. A low weight loss ratio of 1.50 to 1.93% was observed at a restriction temperature <4℃. When T3 with high nitrogen content in the medium was treated at a restriction temperature of 4℃, the fruiting body color values after 31 days of storage were 84.81 (L) and 6.3 (ΔE). This color change was minute compared to that after other treatments. The sensory characteristics score was 5.2 after 31 days of storage, and the quality remained acceptable for consumption.
The purpose of the present study was to investigate weld metallurgical phenomena such as primary solidification mode, microstructural evolution and hot cracking susceptibility in nitrogen-bearing austenitic stainless steel GTA welds. Eight experimental heats varying nitrogen content from 0.007 to 0.23 wt.% were used in this study. Autogenous GTA welding was performed on weld coupons and the primary solidification mode and their microstructural characteristics were investigated from the fusion welds. Varestraint test was employed to evaluate the solidification cracking susceptibility of the heats and TCL(Total Crack Length) was used as cracking susceptibility index. The solidification mode shifted from primary ferrite to primary austenite with an increase in nitrogen content. Retained delta ferrite exhibited a variety of morphology as nitrogen content varied. The weld fusion zone exhibited duplex structure(austenite+ferrite) at nitrogen contents less than 0.10 wt.% but fully austenitic structure at nitrogen contents more than 0.20 wt.%. The weld fusion zone in alloys with about 0.15 wt.% nitrogen experienced primary austenite + primary ferrite solidification (mode AF) and contained delta ferrite less than 1% at room temperature. Regarding to solidification cracking susceptibility, the welds with fully austenitic structure exhibited high cracking susceptibility while those with duplex structure low susceptibility. The cracking susceptibility increased slowly with an increase in nitrogen content up to 0.20 wt.% but sharply as nitrogen content exceeded 0.20 wt.%, which was attributed to solidification mode shift fro primary ferrite to primary austenite single phase solidification.
Transactions on Electrical and Electronic Materials
/
v.14
no.5
/
pp.246-249
/
2013
Graphene was obtained on Cu foil by thermal decomposition method. A gas mixture of $H_2$ and $CH_4$ and an ambient annealing temperature of $1,000^{\circ}C$ were used during the deposition for 30 Min., and for the transfer onto $SiO_2/Si$ and Si substrates. The physical properties of graphene were investigated with regard to the effect ofnitrogen atom doping and the various substrates used. The G/2D ratio decreased when the graphene became monolayer graphene. The graphene grown on $SiO_2/Si$ substrate showed a low intensity of the G/2D ratio, because the polarity of the $SiO_2$ layer improved the quality of graphene. The intensity of the G/2D ratio of graphene doped with nitrogen atoms increased with the doping time. The quality of graphene depended on the concentration of the nitrogen doping and chemical properties of substrates. High-quality monolayer graphene was obtained with a low G/2D ratio. The increase in the intensity of the G/2D ratios corresponded to a blue shift in the 2D peaks.
Kim, Hyun-Jin;Lee, Young-Whan;Choi, Dae-Ki;Lee, Eun-Il
Carbon letters
/
v.3
no.4
/
pp.198-204
/
2002
In this paper, impregnated activated carbon fiber (IACF) was manufactured to pitch-based activated carbon fibers (ACF) with potassium hydroxide (KOH) by using wet impregnation method to raise nitrogen oxides ($NO_x$) adsorptivity. The properties of IACF were observed using EPMA, TGA and DSC and $NO_x$ adsorptivity was observed at high and low temperature. Before and after adsorption was analyzed using ToF-SIMS for examine surface characterization of adsorbed $NO_x$. The results showed that the better adsorptivity appeared for increasing KOH ratio. So, $NO_x$ adsorptivity showed result that is proportional between KOH and the adsorbed amount. On the other hand, adsorbent that manufactured without washing was better $NO_x$ adsorptivity than adsorbent that manufactured with washing. The behavior of adsorption show that crossing time of NO and $NO_2$ delayed for a rising adsorptivity. And NO ratio increased but $NO_2$ ratio decreased according as KOH ratio increases. $NO_x$ was confirmed through surface analysis that remain in $NO_2^-$ and $NO_3^-$ form on IACF surface.
OH, CH and NO radical distributions have been measured and compared with the numerical analysis results in methane/air partially premixed laminar flames using 2-D LIF technique. The pick intensity of OH LIF signal is insensitive to fuel equivalence ratio: however, CH LIF intensity decreases as equivalence ratio increases and the NO concentration increases with equivalence ratio. The contribution of the prompt NO, formed near premixed reaction zone, to the total NO formation is evident from the OH, CH, and NO PLIF images in which the dilution effect of nitrogen is minimal for the highest equivalence ratio. Measured OH and NO LIF signals in counterflow flames agree with the computed concentration distributions. Both numerical and experimental results indicate that the structural change in a flame alters the NO formation characteristics of a partially premixed counterflow flame. The nitrogen dilution also changes flame structure, temperature and OH radical distributions and results in the decreased NO concentrations in a flame. The levels of decrease in NO concentrations, however, depends on the premixedness(${\alpha}$) of a flame. The larger change in the flame structure and NO concentrations have been observed in a premixed flame(${\alpha}=1.0$), which implies that the premixedness is likely to be a factor in the dilution effect on NO formation of a flame.
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